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Protein

Sentrin-specific protease 8

Gene

Senp8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53.1 Publication

Catalytic activityi

Hydrolysis of the alpha-linked peptide bond in the sequence Gly-Gly-|-Ala-Thr-Tyr at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptide, Smt3, leading to the mature form of the protein. A second reaction involves the cleavage of an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei102 – 1021By similarity
Active sitei119 – 1191By similarity
Active sitei163 – 1631NucleophileBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC48.011.

Names & Taxonomyi

Protein namesi
Recommended name:
Sentrin-specific protease 8 (EC:3.4.22.68)
Alternative name(s):
Deneddylase-1
NEDD8-specific protease 1
Sentrin/SUMO-specific protease SENP8
Gene namesi
Name:Senp8
Synonyms:Den1, Nedp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1918849. Senp8.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 221221Sentrin-specific protease 8PRO_0000101728Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9D2Z4.
PaxDbiQ9D2Z4.
PRIDEiQ9D2Z4.

PTM databases

PhosphoSiteiQ9D2Z4.

Expressioni

Gene expression databases

BgeeiQ9D2Z4.
CleanExiMM_SENP8.
GenevisibleiQ9D2Z4. MM.

Interactioni

Protein-protein interaction databases

BioGridi214805. 1 interaction.
STRINGi10090.ENSMUSP00000129441.

Structurei

3D structure databases

ProteinModelPortaliQ9D2Z4.
SMRiQ9D2Z4. Positions 1-211.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni11 – 174164ProteaseAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C48 family.Curated

Phylogenomic databases

eggNOGiKOG3246. Eukaryota.
ENOG4111G37. LUCA.
GeneTreeiENSGT00390000014038.
HOGENOMiHOG000005740.
HOVERGENiHBG054214.
InParanoidiQ9D2Z4.
KOiK08597.
OrthoDBiEOG747PM3.
PhylomeDBiQ9D2Z4.
TreeFamiTF351057.

Family and domain databases

InterProiIPR003653. Peptidase_C48_C.
[Graphical view]
PfamiPF02902. Peptidase_C48. 1 hit.
[Graphical view]
PROSITEiPS50600. ULP_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9D2Z4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPVVLSYMD SLLRQSDVSL LDPPSWLNDH IIGFAFEYFA NSQFHDCSDH
60 70 80 90 100
VCFISPEVTQ FIKCTSSPAE IAMFLEPLDL PHKRVVFLAI NDNSNQAAGG
110 120 130 140 150
THWSLLVYLQ DKNSFFHYDS HSRSNSIHAK QVAEKLKAFL GSKGDKLVFV
160 170 180 190 200
EEKAPAQENS YDCGMYVICN TEALCQSLFR RQPESPLQLL TPTYITKKRG
210 220
EWKDLIARLA KKNEVATEEC S
Length:221
Mass (Da):25,057
Last modified:November 28, 2002 - v2
Checksum:iAF9EB91D8A1C6F39
GO

Sequence cautioni

The sequence BAB31304.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK018606 mRNA. Translation: BAB31304.1. Different initiation.
AK049120 mRNA. Translation: BAC33554.1.
BC037443 mRNA. Translation: AAH37443.1.
CCDSiCCDS23254.1.
RefSeqiNP_001165539.1. NM_001172068.1.
NP_001165540.1. NM_001172069.1.
NP_001165541.1. NM_001172070.1.
NP_001165542.1. NM_001172071.1.
NP_082114.1. NM_027838.3.
UniGeneiMm.279070.

Genome annotation databases

EnsembliENSMUST00000051039; ENSMUSP00000054509; ENSMUSG00000051705.
ENSMUST00000177963; ENSMUSP00000137228; ENSMUSG00000051705.
GeneIDi71599.
KEGGimmu:71599.
UCSCiuc009pym.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK018606 mRNA. Translation: BAB31304.1. Different initiation.
AK049120 mRNA. Translation: BAC33554.1.
BC037443 mRNA. Translation: AAH37443.1.
CCDSiCCDS23254.1.
RefSeqiNP_001165539.1. NM_001172068.1.
NP_001165540.1. NM_001172069.1.
NP_001165541.1. NM_001172070.1.
NP_001165542.1. NM_001172071.1.
NP_082114.1. NM_027838.3.
UniGeneiMm.279070.

3D structure databases

ProteinModelPortaliQ9D2Z4.
SMRiQ9D2Z4. Positions 1-211.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214805. 1 interaction.
STRINGi10090.ENSMUSP00000129441.

Protein family/group databases

MEROPSiC48.011.

PTM databases

PhosphoSiteiQ9D2Z4.

Proteomic databases

MaxQBiQ9D2Z4.
PaxDbiQ9D2Z4.
PRIDEiQ9D2Z4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051039; ENSMUSP00000054509; ENSMUSG00000051705.
ENSMUST00000177963; ENSMUSP00000137228; ENSMUSG00000051705.
GeneIDi71599.
KEGGimmu:71599.
UCSCiuc009pym.2. mouse.

Organism-specific databases

CTDi123228.
MGIiMGI:1918849. Senp8.

Phylogenomic databases

eggNOGiKOG3246. Eukaryota.
ENOG4111G37. LUCA.
GeneTreeiENSGT00390000014038.
HOGENOMiHOG000005740.
HOVERGENiHBG054214.
InParanoidiQ9D2Z4.
KOiK08597.
OrthoDBiEOG747PM3.
PhylomeDBiQ9D2Z4.
TreeFamiTF351057.

Miscellaneous databases

PROiQ9D2Z4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D2Z4.
CleanExiMM_SENP8.
GenevisibleiQ9D2Z4. MM.

Family and domain databases

InterProiIPR003653. Peptidase_C48_C.
[Graphical view]
PfamiPF02902. Peptidase_C48. 1 hit.
[Graphical view]
PROSITEiPS50600. ULP_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cecum.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  3. "Identification and characterization of DEN1, a deneddylase of the ULP family."
    Gan-Erdene T., Nagamalleswari K., Yin L., Wu K., Pan Z.-Q., Wilkinson K.D.
    J. Biol. Chem. 278:28892-28900(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION.
  4. "Specific and covalent targeting of conjugating and deconjugating enzymes of ubiquitin-like proteins."
    Hemelaar J., Borodovsky A., Kessler B.M., Reverter D., Cook J., Kolli N., Gan-Erdene T., Wilkinson K.D., Gill G., Lima C.D., Ploegh H.L., Ovaa H.
    Mol. Cell. Biol. 24:84-95(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiSENP8_MOUSE
AccessioniPrimary (citable) accession number: Q9D2Z4
Secondary accession number(s): Q543P0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: November 28, 2002
Last modified: June 8, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.