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Protein

Vacuolar protein sorting-associated protein 33A

Gene

Vps33a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for fusion of endosomes and autophagosomes with lysosomes; the function is dependent on its association with VPS16 but not VIPAS39. The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG.By similarity

GO - Biological processi

  • autophagosome maturation Source: MGI
  • endosome to lysosome transport Source: MGI
  • lysosome localization Source: MGI
  • melanosome localization Source: MGI
  • pigmentation Source: MGI
  • platelet formation Source: MGI
  • protein transport Source: UniProtKB-KW
  • regulation of developmental pigmentation Source: MGI
  • vesicle docking involved in exocytosis Source: InterPro
  • vesicle-mediated transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 33A
Gene namesi
Name:Vps33a
Synonyms:Bf, Buff
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1924823. Vps33a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Lysosome, Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in Vps33a are the cause of the buff mutant which exhibits hypopigmentation in the coat and eyes, due to reduced size and number of melanosomes in their cells. In addition, mice are prone to prolonged bleeding, due to a platelet-storage pool defect.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 598598Vacuolar protein sorting-associated protein 33APRO_0000206303Add
BLAST

Proteomic databases

EPDiQ9D2N9.
MaxQBiQ9D2N9.
PaxDbiQ9D2N9.
PeptideAtlasiQ9D2N9.
PRIDEiQ9D2N9.

PTM databases

iPTMnetiQ9D2N9.
PhosphoSiteiQ9D2N9.

Expressioni

Gene expression databases

BgeeiQ9D2N9.
CleanExiMM_VPS33A.
ExpressionAtlasiQ9D2N9. baseline and differential.
GenevisibleiQ9D2N9. MM.

Interactioni

Subunit structurei

Core component of at least two putative endosomal tethering complexes, the homotypic fusion and vacuole protein sorting (HOPS) complex and the class C core vacuaole/endosome tethering (CORVET) complex. Their common core is composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, which in HOPS further associates with VPS39 and VPS41 and in CORVET with VPS8 and TGFBRAP1 (PubMed:25266290). Interacts with RAB5C (PubMed:25266290). Interacts with UVRAG, STX17, MON1A and MON1B (By similarity). Associates with adaptor protein complex 3 (AP-3) and clathrin (By similarity).By similarity1 Publication1 Publication

Protein-protein interaction databases

BioGridi218770. 1 interaction.
IntActiQ9D2N9. 2 interactions.
STRINGi10090.ENSMUSP00000031388.

Structurei

3D structure databases

ProteinModelPortaliQ9D2N9.
SMRiQ9D2N9. Positions 2-598.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the STXBP/unc-18/SEC1 family.Curated

Phylogenomic databases

eggNOGiKOG1302. Eukaryota.
COG5158. LUCA.
GeneTreeiENSGT00530000063488.
HOGENOMiHOG000166771.
HOVERGENiHBG054244.
InParanoidiQ9D2N9.
KOiK20182.
OMAiRHVANMM.
OrthoDBiEOG722J85.
TreeFamiTF315126.

Family and domain databases

Gene3Di3.40.50.1910. 2 hits.
InterProiIPR027482. Sec-1-like_dom2.
IPR001619. Sec1-like.
[Graphical view]
PANTHERiPTHR11679. PTHR11679. 1 hit.
PfamiPF00995. Sec1. 1 hit.
[Graphical view]
SUPFAMiSSF56815. SSF56815. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9D2N9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAHLSYGRV NLNVLREAVR RELREFLDKC AGSKAIVWDE YLTGPFGLIA
60 70 80 90 100
QYSLLKEHEV EKMFTLKGSR LPAADVKNII FLVRPRLELM DIIAENVLSE
110 120 130 140 150
DRRGPTRDFH ILFVPRRSLL CEQRLKDLGV LGSFIHREEY SLDLIPFDGD
160 170 180 190 200
LLSMESEGAF KECYLEGDQT SLYHAAKGLM TLQALYGTIP QIFGKGECAR
210 220 230 240 250
QVANMMVRMK REFTGSQNSV FPVFDNLLLL DRNVDLLTPL ASQLTYEGLI
260 270 280 290 300
DEIYGIQNSY VKLPPEKFAP KKQGGGSGGK DLPTEAKKLQ LNSAEELYAE
310 320 330 340 350
IRDKNFNAVG SVLSKKAKII SAAFEERHNA KTVGEIKQFV SQLPHMQAAR
360 370 380 390 400
GSLANHTSIA ELIKDVTTSE DFFDKLTVEQ EFMSGIDTDK VNSYIEDCIA
410 420 430 440 450
QKHPLIKVLR LVCLQSVCNS GLKQKVLDYY RREILQTYGY EHILTLNNLE
460 470 480 490 500
KAGLLKAQTG GRNNYPTIRK TLRLWMDDVN EQNPTDISYV YSGYAPLSVR
510 520 530 540 550
LAQLLSRPGW RSIEEVLRIL PGPHFEERQP LPTGLQKKRQ PGENRVTLVF
560 570 580 590
FLGGVTFAEI AALRFLSQLE DGGTEYVIAT TKLMNGNSWI EALMEKPF
Length:598
Mass (Da):67,555
Last modified:July 27, 2011 - v2
Checksum:i7311BEA79D6C90FA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti128 – 1281L → V in BAB31735 (PubMed:16141072).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti251 – 2511D → E Detected in mice with the buff mutation; leads to melanocytes with a reduced number of undermelanized melanosomes. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF439858 mRNA. Translation: AAL33578.1.
AK019463 mRNA. Translation: BAB31735.1.
AK041982 mRNA. Translation: BAC31121.1.
AK140553 mRNA. Translation: BAE24421.1.
AK150282 mRNA. Translation: BAE29438.1.
AK150550 mRNA. Translation: BAE29652.1.
CCDSiCCDS19666.1.
RefSeqiNP_084205.3. NM_029929.3.
UniGeneiMm.41372.

Genome annotation databases

EnsembliENSMUST00000031388; ENSMUSP00000031388; ENSMUSG00000029434.
GeneIDi77573.
KEGGimmu:77573.
UCSCiuc008znz.2. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF439858 mRNA. Translation: AAL33578.1.
AK019463 mRNA. Translation: BAB31735.1.
AK041982 mRNA. Translation: BAC31121.1.
AK140553 mRNA. Translation: BAE24421.1.
AK150282 mRNA. Translation: BAE29438.1.
AK150550 mRNA. Translation: BAE29652.1.
CCDSiCCDS19666.1.
RefSeqiNP_084205.3. NM_029929.3.
UniGeneiMm.41372.

3D structure databases

ProteinModelPortaliQ9D2N9.
SMRiQ9D2N9. Positions 2-598.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218770. 1 interaction.
IntActiQ9D2N9. 2 interactions.
STRINGi10090.ENSMUSP00000031388.

PTM databases

iPTMnetiQ9D2N9.
PhosphoSiteiQ9D2N9.

Proteomic databases

EPDiQ9D2N9.
MaxQBiQ9D2N9.
PaxDbiQ9D2N9.
PeptideAtlasiQ9D2N9.
PRIDEiQ9D2N9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031388; ENSMUSP00000031388; ENSMUSG00000029434.
GeneIDi77573.
KEGGimmu:77573.
UCSCiuc008znz.2. mouse.

Organism-specific databases

CTDi65082.
MGIiMGI:1924823. Vps33a.

Phylogenomic databases

eggNOGiKOG1302. Eukaryota.
COG5158. LUCA.
GeneTreeiENSGT00530000063488.
HOGENOMiHOG000166771.
HOVERGENiHBG054244.
InParanoidiQ9D2N9.
KOiK20182.
OMAiRHVANMM.
OrthoDBiEOG722J85.
TreeFamiTF315126.

Miscellaneous databases

ChiTaRSiVps33a. mouse.
PROiQ9D2N9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D2N9.
CleanExiMM_VPS33A.
ExpressionAtlasiQ9D2N9. baseline and differential.
GenevisibleiQ9D2N9. MM.

Family and domain databases

Gene3Di3.40.50.1910. 2 hits.
InterProiIPR027482. Sec-1-like_dom2.
IPR001619. Sec1-like.
[Graphical view]
PANTHERiPTHR11679. PTHR11679. 1 hit.
PfamiPF00995. Sec1. 1 hit.
[Graphical view]
SUPFAMiSSF56815. SSF56815. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse organellar biogenesis mutant buff results from a mutation in Vps33a, a homologue of yeast vps33 and Drosophila carnation."
    Suzuki T., Oiso N., Gautam R., Novak E.K., Panthier J.J., Suprabha P.G., Vida T., Swank R.T., Spritz R.A.
    Proc. Natl. Acad. Sci. U.S.A. 100:1146-1150(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ROLE IN DISEASE, VARIANT GLU-251, CHARACTERIZATION OF VARIANT GLU-251.
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone marrow, Cerebellum, Embryo and Placenta.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung, Spleen and Testis.
  4. "Mammalian CORVET is required for fusion and conversion of distinct early endosome subpopulations."
    Perini E.D., Schaefer R., Stoeter M., Kalaidzidis Y., Zerial M.
    Traffic 15:1366-1389(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAB5C, SUBUNIT.

Entry informationi

Entry nameiVP33A_MOUSE
AccessioniPrimary (citable) accession number: Q9D2N9
Secondary accession number(s): Q8BGT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.