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Q9D2N4 (DTNA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dystrobrevin alpha

Short name=DTN-A
Alternative name(s):
Alpha-dystrobrevin
Gene names
Name:Dtna
Synonyms:Dtn
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length746 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in synapse maturation and required for normal muscle function.

Subunit structure

Interacts with dystrophin, utrophin and the syntrophins SNTA1, SNTB1, SNTB2, SNTG1 and SNTG2. Isoforms 5 and 6 do not interact with syntrophin. Isoforms 3 and 4 do not interact with utrophin. Binds dystrobrevin binding protein 1. Interacts with MAGEE1. Ref.6 Ref.7 Ref.8 Ref.9

Subcellular location

Cytoplasm. Cell junctionsynapse. Cell membrane. Note: In peripheral nerves, colocalizes with MAGEE1 in the Schwann cell membrane. Ref.9

Tissue specificity

Expressed in skeletal muscle, heart, lung and brain. Sarcolemma and neuromuscular junction in skeletal muscle. Isoform 2 is restricted to the neuromuscular junction. Isoforms 5 and 6 are only expressed in muscle.

Developmental stage

Expression of alpha-dystrobrevin is up-regulated during differentiation, with isoforms 2, 5 and 6 expressed earliest and isoform 3 and 4 expressed later.

Domain

The coiled coil domain mediates the interaction with dystrophin and utrophin.

Post-translational modification

Phosphorylation of isoform 2 on tyrosine kinase substrate domain present in the C-terminus.

Sequence similarities

Belongs to the dystrophin family. Dystrobrevin subfamily.

Contains 1 ZZ-type zinc finger.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Dtnbp1Q91WZ83EBI-296019,EBI-643186

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q9D2N4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9D2N4-2)

Also known as: Alpha-dystrobrevin-1; Alpha-DB1;

The sequence of this isoform differs from the canonical sequence as follows:
     363-419: Missing.
     560-561: QG → R
Isoform 3 (identifier: Q9D2N4-3)

Also known as: Alpha-dystrobrevin-2B; Alpha-DB2B;

The sequence of this isoform differs from the canonical sequence as follows:
     559-570: TQGASSPRSSPS → VMHEIIPLEERT
     571-746: Missing.
Isoform 4 (identifier: Q9D2N4-4)

Also known as: Alpha-dystrobrevin-2A; Alpha-DB2A;

The sequence of this isoform differs from the canonical sequence as follows:
     559-567: TQGASSPRS → VSYVPYCRS
     568-746: Missing.
Isoform 5 (identifier: Q9D2N4-5)

Also known as: Alpha-dystrobrevin-3; Alpha-DB3;

The sequence of this isoform differs from the canonical sequence as follows:
     334-334: V → VDTW
     364-371: PPKDSEVE → DGAHGGCV
     372-746: Missing.
Isoform 6 (identifier: Q9D2N4-6)

Also known as: Alpha-dystrobrevin-3; Alpha-DB3;

The sequence of this isoform differs from the canonical sequence as follows:
     364-371: PPKDSEVE → DGAHGGCV
     372-746: Missing.
Isoform 7 (identifier: Q9D2N4-7)

The sequence of this isoform differs from the canonical sequence as follows:
     334-334: V → VDTW
     363-419: Missing.
     559-567: TQGASSPRS → VSYVPYCRS
     568-746: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 746746Dystrobrevin alpha
PRO_0000080037

Regions

Zinc finger237 – 28448ZZ-type
Region1 – 288288Interaction with MAGEE1
Region397 – 44751Syntrophin-binding region
Coiled coil458 – 557100 Potential

Natural variations

Alternative sequence3341V → VDTW in isoform 5 and isoform 7.
VSP_004214
Alternative sequence363 – 41957Missing in isoform 2 and isoform 7.
VSP_004215
Alternative sequence364 – 3718PPKDSEVE → DGAHGGCV in isoform 5 and isoform 6.
VSP_004216
Alternative sequence372 – 746375Missing in isoform 5 and isoform 6.
VSP_004217
Alternative sequence559 – 57012TQGAS…RSSPS → VMHEIIPLEERT in isoform 3.
VSP_004221
Alternative sequence559 – 5679TQGASSPRS → VSYVPYCRS in isoform 4 and isoform 7.
VSP_004218
Alternative sequence560 – 5612QG → R in isoform 2.
VSP_004220
Alternative sequence568 – 746179Missing in isoform 4 and isoform 7.
VSP_004219
Alternative sequence571 – 746176Missing in isoform 3.
VSP_004222

Experimental info

Sequence conflict2411V → L in CAA64519. Ref.1
Sequence conflict2511M → I in BAB31746. Ref.5
Sequence conflict2591Q → P in AAD33915. Ref.4
Sequence conflict4161D → N in AAD33915. Ref.4
Sequence conflict4161D → N in AAD33914. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 8, 2011. Version 2.
Checksum: 6AB7EF3B69250A09

FASTA74684,067
        10         20         30         40         50         60 
MIEDSGKRGN TMAERRQLFA EMRAQDLDRI RLSTYRTACK LRFVQKKCNL HLVDIWNVIE 

        70         80         90        100        110        120 
ALRENALNNL DPNIELNVAR LEAVLSTIFY QLNKRMPTTH QIHVEQSISL LLNFLLAAFD 

       130        140        150        160        170        180 
PEGHGKISVF AVKMALATLC GGKIMDKLRY IFSMISDSSG VMVYGRYDQF LREVLKLPTA 

       190        200        210        220        230        240 
VFEGPSFGYT EQSARSCFSQ QKKVTLNGFL DTLMSDPPPQ CLVWLPLLHR LANVENVFHP 

       250        260        270        280        290        300 
VECSYCHSES MMGFRYRCQQ CHNYQLCQDC FWRGHAGGSH SNQHQMKEYT SWKSPAKKLT 

       310        320        330        340        350        360 
NALSKSLSCA SSREPLHPMF PDQPEKPLNL AHIVPPRPVT SMNDTLFSHS VPSSGSPFIT 

       370        380        390        400        410        420 
RSSPPKDSEV EQNKMLARAA PAFLKGRGIQ YSLNVADRLA DEHVLIGLYV NMLRNDPPCM 

       430        440        450        460        470        480 
LESSNRLDEE HRLIARYAAR LAAESSSSQP TQQRSAPDIS FTIDANKQQR QLIAELENKN 

       490        500        510        520        530        540 
REILQEIQRL RVEHEQASQP TPEKAQQNPT LLAELRLLRQ RKDELEQRMS ALQESRRELM 

       550        560        570        580        590        600 
VQLEGLMKLL KEEELKQGTQ GASSPRSSPS HTISRPIPMP IRSASACPTP THTPQDSLTG 

       610        620        630        640        650        660 
VGGDVQEAFA QSSRRNLRSD LLVAADSITN TMSSLVKELN SEVASETEST VDSEFSRPQF 

       670        680        690        700        710        720 
EDLAPSPTSE KAFLAQIHSR KPGYIHGGAA STTHGDMVPE DGDPYTQPED GNYENESVRQ 

       730        740 
LENELQLEEY LKQKLQDEAY QVSLQG 

« Hide

Isoform 2 (Alpha-dystrobrevin-1) (Alpha-DB1) [UniParc].

Checksum: 82AC1104D48313EA
Show »

FASTA68877,733
Isoform 3 (Alpha-dystrobrevin-2B) (Alpha-DB2B) [UniParc].

Checksum: CA135BFB7B7A3DDE
Show »

FASTA57065,157
Isoform 4 (Alpha-dystrobrevin-2A) (Alpha-DB2A) [UniParc].

Checksum: 73C843F6C9E27940
Show »

FASTA56764,763
Isoform 5 (Alpha-dystrobrevin-3) (Alpha-DB3) [UniParc].

Checksum: 286DE068A207D235
Show »

FASTA37442,380
Isoform 6 (Alpha-dystrobrevin-3) (Alpha-DB3) [UniParc].

Checksum: 444BB5947F3D5DD3
Show »

FASTA37141,977
Isoform 7 [UniParc].

Checksum: EA3913DA5334D7E1
Show »

FASTA51358,860

References

« Hide 'large scale' references
[1]"Isoform diversity of dystrobrevin, the murine 87-kDa postsynaptic protein."
Blake D.J., Nawrotzki R., Peters M.F., Froehner S.C., Davies K.E.
J. Biol. Chem. 271:7802-7810(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 7).
Tissue: Brain.
[2]"Genomic organization of the mouse dystrobrevin gene: comparative analysis with the dystrophin gene."
Ambrose H.J., Blake D.J., Nawrotzki R.A., Davies K.E.
Genomics 39:359-369(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: 129/Sv.
Tissue: Brain.
[3]"Characterisation of alpha-dystrobrevin in muscle."
Nawrotzki R., Loh N.Y., Ruegg M.A., Davies K.E., Blake D.J.
J. Cell Sci. 111:2595-2605(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5).
Tissue: Muscle.
[4]"Differential expression and developmental regulation of a novel alpha-dystrobrevin isoform in muscle."
Enigk R.E., Maimone M.M.
Gene 238:479-488(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3; 4 AND 6).
Strain: C3H.
Tissue: Muscle.
[5]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Skin.
[6]"Interactions between dystrophin glycoprotein complex proteins."
Madhavan R., Jarrett H.W.
Biochemistry 34:12204-12209(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SNTA1.
[7]"Differential association of syntrophin pairs with the dystrophin complex."
Peters M.F., Adams M.E., Froehner S.C.
J. Cell Biol. 138:81-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SNTB1.
[8]"Dysbindin, a novel coiled-coil-containing protein that interacts with the dystrobrevins in muscle and brain."
Benson M.A., Newey S.E., Martin-Rendon E., Hawkes R., Blake D.J.
J. Biol. Chem. 276:24232-24241(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DYSTROBREVIN BINDING PROTEIN 1.
[9]"DAMAGE, a novel alpha-dystrobrevin-associated MAGE protein in dystrophin complexes."
Albrecht D.E., Froehner S.C.
J. Biol. Chem. 279:7014-7023(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH MAGEE1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X95226 mRNA. Translation: CAA64518.1.
X95227 mRNA. Translation: CAA64519.1.
Z79787 expand/collapse EMBL AC list , Z79788, Z79789, Z79790, Z79791, Z79792, Z79793, Z79794, Z79795, Z79796, Z79797 Genomic DNA. Translation: CAB02145.1.
AJ009669 mRNA. Translation: CAA08770.1.
AF143544 mRNA. Translation: AAD33915.1.
AF143543 mRNA. Translation: AAD33914.1.
AF143542 mRNA. Translation: AAD33913.1.
AK019477 mRNA. Translation: BAB31746.1.
RefSeqNP_001272736.1. NM_001285807.1.
NP_001272739.1. NM_001285810.1.
NP_001272746.1. NM_001285817.1.
NP_034217.2. NM_010087.4.
XP_006525682.1. XM_006525619.1.
XP_006525685.1. XM_006525622.1.
UniGeneMm.484047.
Mm.94371.

3D structure databases

ProteinModelPortalQ9D2N4.
SMRQ9D2N4. Positions 26-235, 237-292.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9D2N4. 6 interactions.
MINTMINT-197169.

PTM databases

PhosphoSiteQ9D2N4.

Proteomic databases

PaxDbQ9D2N4.
PRIDEQ9D2N4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000047954; ENSMUSP00000037475; ENSMUSG00000024302. [Q9D2N4-6]
GeneID13527.
KEGGmmu:13527.
UCSCuc008efs.1. mouse. [Q9D2N4-7]

Organism-specific databases

CTD1837.
MGIMGI:106039. Dtna.

Phylogenomic databases

eggNOGNOG251970.
GeneTreeENSGT00740000115370.
HOVERGENHBG005539.
InParanoidQ9D2N4.
PhylomeDBQ9D2N4.

Gene expression databases

ArrayExpressQ9D2N4.
BgeeQ9D2N4.
CleanExMM_DTNA.
GenevestigatorQ9D2N4.

Family and domain databases

Gene3D1.10.238.10. 2 hits.
InterProIPR017432. Distrobrevin.
IPR011992. EF-hand-dom_pair.
IPR015153. EF-hand_dom_typ1.
IPR015154. EF-hand_dom_typ2.
IPR000433. Znf_ZZ.
[Graphical view]
PfamPF09068. EF-hand_2. 1 hit.
PF09069. EF-hand_3. 1 hit.
PF00569. ZZ. 1 hit.
[Graphical view]
PIRSFPIRSF038204. Distrobrevin. 1 hit.
SMARTSM00291. ZnF_ZZ. 1 hit.
[Graphical view]
PROSITEPS01357. ZF_ZZ_1. 1 hit.
PS50135. ZF_ZZ_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio284112.
PROQ9D2N4.
SOURCESearch...

Entry information

Entry nameDTNA_MOUSE
AccessionPrimary (citable) accession number: Q9D2N4
Secondary accession number(s): P97319 expand/collapse secondary AC list , Q61498, Q61499, Q9QZZ5, Q9WUL9, Q9WUM0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 8, 2011
Last modified: April 16, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot