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Protein

Ubiquitin-conjugating enzyme E2 variant 2

Gene

Ube2v2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has no ubiquitin ligase activity on its own. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-MMU-5693571. Nonhomologous End-Joining (NHEJ).
R-MMU-5693607. Processing of DNA double-strand break ends.
R-MMU-5696395. Formation of Incision Complex in GG-NER.
R-MMU-69473. G2/M DNA damage checkpoint.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 variant 2
Alternative name(s):
Ubc-like protein MMS2
Gene namesi
Name:Ube2v2
Synonyms:Mms2, Uev2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1917870. Ube2v2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 145144Ubiquitin-conjugating enzyme E2 variant 2PRO_0000082603Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9D2M8.
MaxQBiQ9D2M8.
PaxDbiQ9D2M8.
PeptideAtlasiQ9D2M8.
PRIDEiQ9D2M8.

PTM databases

iPTMnetiQ9D2M8.
PhosphoSiteiQ9D2M8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022674.
CleanExiMM_UBE2V2.
ExpressionAtlasiQ9D2M8. baseline and differential.
GenevisibleiQ9D2M8. MM.

Interactioni

Subunit structurei

Heterodimer with UBE2N. Binds CHFR (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi214171. 3 interactions.
IntActiQ9D2M8. 4 interactions.
STRINGi10090.ENSMUSP00000111443.

Structurei

3D structure databases

ProteinModelPortaliQ9D2M8.
SMRiQ9D2M8. Positions 6-145.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0896. Eukaryota.
ENOG4111MDW. LUCA.
GeneTreeiENSGT00740000115534.
HOVERGENiHBG054552.
InParanoidiQ9D2M8.
KOiK10704.
OMAiISMNGIN.
OrthoDBiEOG091G0SMT.
PhylomeDBiQ9D2M8.
TreeFamiTF316971.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D2M8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVSTGVKVP RNFRLLEELE EGQKGVGDGT VSWGLEDDED MTLTRWTGMI
60 70 80 90 100
IGPPRTNYEN RIYSLKVECG SKYPEAPPSV RFVTKINMNG INNSSGMVDA
110 120 130 140
RSIPVLAKWQ NSYSIKVILQ ELRRLMMSKE NMKLPQPPEG QTYNN
Length:145
Mass (Da):16,367
Last modified:January 23, 2007 - v4
Checksum:i98D63173902C6611
GO
Isoform 2 (identifier: Q9D2M8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-97: Missing.

Show »
Length:103
Mass (Da):11,674
Checksum:i8EF809082980A3DE
GO

Sequence cautioni

The sequence AAH29742 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161L → W in BAC26094 (PubMed:16141072).Curated
Sequence conflicti38 – 381D → N in BAC25968 (PubMed:16141072).Curated
Sequence conflicti38 – 381D → N in BAC27311 (PubMed:16141072).Curated
Sequence conflicti38 – 381D → N in BAC28128 (PubMed:16141072).Curated
Sequence conflicti38 – 381D → N in BAB31753 (PubMed:19468303).Curated
Sequence conflicti76 – 761A → D in AAG22084 (PubMed:11406273).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei56 – 9742Missing in isoform 2. 1 PublicationVSP_011529Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF303828 mRNA. Translation: AAG22084.1.
AK017786 mRNA. Translation: BAB30932.1.
AK019486 mRNA. Translation: BAB31753.1.
AK028472 mRNA. Translation: BAC25968.1.
AK028742 mRNA. Translation: BAC26094.1.
AK031233 mRNA. Translation: BAC27311.1.
AK033016 mRNA. Translation: BAC28128.1.
CT010522 Genomic DNA. Translation: CAO77942.1.
BC029742 mRNA. Translation: AAH29742.1. Different initiation.
BC058374 mRNA. Translation: AAH58374.1.
BC083098 mRNA. Translation: AAH83098.1.
CCDSiCCDS37265.1. [Q9D2M8-1]
CCDS49771.1. [Q9D2M8-2]
RefSeqiNP_001152823.1. NM_001159351.1. [Q9D2M8-2]
NP_076074.2. NM_023585.4. [Q9D2M8-1]
UniGeneiMm.235407.
Mm.390775.

Genome annotation databases

EnsembliENSMUST00000115776; ENSMUSP00000111442; ENSMUSG00000022674. [Q9D2M8-2]
ENSMUST00000115777; ENSMUSP00000111443; ENSMUSG00000022674. [Q9D2M8-1]
GeneIDi70620.
KEGGimmu:70620.
UCSCiuc007yhp.2. mouse. [Q9D2M8-1]
uc007yhq.2. mouse. [Q9D2M8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF303828 mRNA. Translation: AAG22084.1.
AK017786 mRNA. Translation: BAB30932.1.
AK019486 mRNA. Translation: BAB31753.1.
AK028472 mRNA. Translation: BAC25968.1.
AK028742 mRNA. Translation: BAC26094.1.
AK031233 mRNA. Translation: BAC27311.1.
AK033016 mRNA. Translation: BAC28128.1.
CT010522 Genomic DNA. Translation: CAO77942.1.
BC029742 mRNA. Translation: AAH29742.1. Different initiation.
BC058374 mRNA. Translation: AAH58374.1.
BC083098 mRNA. Translation: AAH83098.1.
CCDSiCCDS37265.1. [Q9D2M8-1]
CCDS49771.1. [Q9D2M8-2]
RefSeqiNP_001152823.1. NM_001159351.1. [Q9D2M8-2]
NP_076074.2. NM_023585.4. [Q9D2M8-1]
UniGeneiMm.235407.
Mm.390775.

3D structure databases

ProteinModelPortaliQ9D2M8.
SMRiQ9D2M8. Positions 6-145.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214171. 3 interactions.
IntActiQ9D2M8. 4 interactions.
STRINGi10090.ENSMUSP00000111443.

PTM databases

iPTMnetiQ9D2M8.
PhosphoSiteiQ9D2M8.

Proteomic databases

EPDiQ9D2M8.
MaxQBiQ9D2M8.
PaxDbiQ9D2M8.
PeptideAtlasiQ9D2M8.
PRIDEiQ9D2M8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000115776; ENSMUSP00000111442; ENSMUSG00000022674. [Q9D2M8-2]
ENSMUST00000115777; ENSMUSP00000111443; ENSMUSG00000022674. [Q9D2M8-1]
GeneIDi70620.
KEGGimmu:70620.
UCSCiuc007yhp.2. mouse. [Q9D2M8-1]
uc007yhq.2. mouse. [Q9D2M8-2]

Organism-specific databases

CTDi7336.
MGIiMGI:1917870. Ube2v2.

Phylogenomic databases

eggNOGiKOG0896. Eukaryota.
ENOG4111MDW. LUCA.
GeneTreeiENSGT00740000115534.
HOVERGENiHBG054552.
InParanoidiQ9D2M8.
KOiK10704.
OMAiISMNGIN.
OrthoDBiEOG091G0SMT.
PhylomeDBiQ9D2M8.
TreeFamiTF316971.

Enzyme and pathway databases

ReactomeiR-MMU-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-MMU-5693571. Nonhomologous End-Joining (NHEJ).
R-MMU-5693607. Processing of DNA double-strand break ends.
R-MMU-5696395. Formation of Incision Complex in GG-NER.
R-MMU-69473. G2/M DNA damage checkpoint.

Miscellaneous databases

PROiQ9D2M8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022674.
CleanExiMM_UBE2V2.
ExpressionAtlasiQ9D2M8. baseline and differential.
GenevisibleiQ9D2M8. MM.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUB2V2_MOUSE
AccessioniPrimary (citable) accession number: Q9D2M8
Secondary accession number(s): A6X924
, Q8BGH6, Q8CE99, Q8K2V7, Q9CYD7, Q9ERI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 131 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.