Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dynein assembly factor 1, axonemal

Gene

Dnaaf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cilium-specific protein required for the stability of the ciliary architecture. Plays a role in cytoplasmic preassembly of dynein arms (By similarity). Involved in regulation of microtubule-based cilia and actin-based brush border microvilli (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Dynein assembly factor 1, axonemal
Alternative name(s):
Leucine-rich repeat-containing protein 50
Gene namesi
Name:Dnaaf1
Synonyms:Lrrc50
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1915520. Dnaaf1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 634634Dynein assembly factor 1, axonemalPRO_0000232890Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei349 – 3491PhosphoserineBy similarity
Modified residuei462 – 4621PhosphothreonineCombined sources
Modified residuei465 – 4651PhosphoserineCombined sources
Modified residuei488 – 4881PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9D2H9.
PaxDbiQ9D2H9.
PRIDEiQ9D2H9.

PTM databases

PhosphoSiteiQ9D2H9.

Expressioni

Gene expression databases

BgeeiQ9D2H9.
CleanExiMM_LRRC50.
GenevisibleiQ9D2H9. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000090790.

Structurei

3D structure databases

ProteinModelPortaliQ9D2H9.
SMRiQ9D2H9. Positions 71-296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati101 – 12323LRR 1Add
BLAST
Repeati124 – 14522LRR 2Add
BLAST
Repeati146 – 16722LRR 3Add
BLAST
Repeati168 – 18922LRR 4Add
BLAST
Repeati190 – 21122LRR 5Add
BLAST
Repeati215 – 23622LRR 6Add
BLAST
Domaini249 – 28840LRRCTAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi392 – 3954Poly-Val

Sequence similaritiesi

Belongs to the DNAAF1 family.Curated
Contains 6 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiENOG410IG8N. Eukaryota.
ENOG410XQAN. LUCA.
GeneTreeiENSGT00730000110223.
HOVERGENiHBG066969.
InParanoidiQ9D2H9.
KOiK19750.
OMAiCLFLQVN.
OrthoDBiEOG7HQN7P.
PhylomeDBiQ9D2H9.
TreeFamiTF315818.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR027734. DNAAF1.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
[Graphical view]
PANTHERiPTHR24365:SF315. PTHR24365:SF315. 2 hits.
PROSITEiPS51450. LRR. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9D2H9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPEVSEPPV DSVAEPSLEE SAGDHGDAGP GIRKEEISET KETCAGPCTT
60 70 80 90 100
SCPSQQQPSG DNGSEGFCTH SRDDREDRGP RMTKQFLQKL CKQHKLYVTP
110 120 130 140 150
ALNDTLYLHF KGFDRIENLE EYTGLRCLWL ECNGIQRIEN LQAQSELRCL
160 170 180 190 200
FLQVNLLHKI ENLEPLQKLD ALNLSNNYIK TIENLSCLPV LNTLQMAHNR
210 220 230 240 250
LETVADIEHL RECLRLCVLD LSHNALSDPE ILSVLESMPC LRVLNLMGNP
260 270 280 290 300
VTKHIPNYRR TVTVRLKHLT YLDDRPVFPK DRACAEAWAR GGYAAEKEER
310 320 330 340 350
RQWESREHKK ITDSLEALAM IKRRAEERKK ARDRGETPLP DSEGSIPTSP
360 370 380 390 400
EAEEKQPMGE IQKKMELFVE ESFEAKDELF PETPGGEKEL HVVVVNGAVE
410 420 430 440 450
NPDLSGSLAH NQTPVVVTPE ESTSPVAATD GARTEDIEAV AVEIKERLFI
460 470 480 490 500
DDLPDLEDAE GTDVSVEDQI KETDIPRIQA ISSLSDDSDP ELDELSLSTS
510 520 530 540 550
EATPTGATGA LSHIFAISKG PSTAATVPFT DICKPIATTD LESQGQDCGA
560 570 580 590 600
AASRPLIQEL NDEPAEEAAN QPLPPQTCAS DPALAHPSED GDSDSQLPAA
610 620 630
TLLGDGAEDE AESSVHPKEP STRVGLEDIE FGLD
Length:634
Mass (Da):69,704
Last modified:June 1, 2001 - v1
Checksum:i4B174F328D433754
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006656 mRNA. Translation: BAB24692.1.
AK019633 mRNA. Translation: BAB31818.1.
BC050751 mRNA. Translation: AAH50751.1.
CCDSiCCDS22709.1.
RefSeqiNP_080924.1. NM_026648.4.
UniGeneiMm.41977.
Mm.475128.

Genome annotation databases

EnsembliENSMUST00000093100; ENSMUSP00000090790; ENSMUSG00000031831.
GeneIDi68270.
KEGGimmu:68270.
UCSCiuc009npw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006656 mRNA. Translation: BAB24692.1.
AK019633 mRNA. Translation: BAB31818.1.
BC050751 mRNA. Translation: AAH50751.1.
CCDSiCCDS22709.1.
RefSeqiNP_080924.1. NM_026648.4.
UniGeneiMm.41977.
Mm.475128.

3D structure databases

ProteinModelPortaliQ9D2H9.
SMRiQ9D2H9. Positions 71-296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000090790.

PTM databases

PhosphoSiteiQ9D2H9.

Proteomic databases

MaxQBiQ9D2H9.
PaxDbiQ9D2H9.
PRIDEiQ9D2H9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000093100; ENSMUSP00000090790; ENSMUSG00000031831.
GeneIDi68270.
KEGGimmu:68270.
UCSCiuc009npw.1. mouse.

Organism-specific databases

CTDi123872.
MGIiMGI:1915520. Dnaaf1.

Phylogenomic databases

eggNOGiENOG410IG8N. Eukaryota.
ENOG410XQAN. LUCA.
GeneTreeiENSGT00730000110223.
HOVERGENiHBG066969.
InParanoidiQ9D2H9.
KOiK19750.
OMAiCLFLQVN.
OrthoDBiEOG7HQN7P.
PhylomeDBiQ9D2H9.
TreeFamiTF315818.

Miscellaneous databases

ChiTaRSiDnaaf1. mouse.
NextBioi326874.
PROiQ9D2H9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D2H9.
CleanExiMM_LRRC50.
GenevisibleiQ9D2H9. MM.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR027734. DNAAF1.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
[Graphical view]
PANTHERiPTHR24365:SF315. PTHR24365:SF315. 2 hits.
PROSITEiPS51450. LRR. 6 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-462; SER-465 AND SER-488, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiDAAF1_MOUSE
AccessioniPrimary (citable) accession number: Q9D2H9
Secondary accession number(s): Q9CVS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: June 1, 2001
Last modified: March 16, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.