Q9D2H6 (SP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor Sp2

Gene names

Name:	Sp2
Synonyms:	Kiaa0048

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the Sp1 C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers.
Sequence caution	The sequence AAH21759.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH86457.1 differs from that shown. Reason: Erroneous initiation. The sequence BAD32155.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cardiovascular system development Inferred from mutant phenotype PubMed 20221402. Source: MGI embryonic organ development Inferred from mutant phenotype PubMed 20221402. Source: MGI fibroblast proliferation Inferred from mutant phenotype PubMed 20221402. Source: MGI in utero embryonic development Inferred from mutant phenotype PubMed 20221402. Source: MGI multicellular organism growth Inferred from mutant phenotype PubMed 20221402. Source: MGI regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from sequence or structural similarity PubMed 15820306. Source: MGI zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9D2H6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9D2H6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-7: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 612	612	Transcription factor Sp2		PRO_0000269192
Regions
	Zinc finger	524 – 548	25	C2H2-type 1
	Zinc finger	554 – 578	25	C2H2-type 2
	Zinc finger	584 – 606	23	C2H2-type 3
Amino acid modifications
	Modified residue	78	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 7	7	Missing in isoform 2.		VSP_022022
Experimental info
	Sequence conflict	6	1	M → T in AAH86457. Ref.3
	Sequence conflict	97	1	Missing in BAD32155. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: FF2778DC55B4044F	FASTA	612	64,908
	10 20 30 40 50 60 MSDPQMSMAA TAAVSPSDYL QPAAATTQDS QPSPLALLAA TCSKIGPPAV EAAVTPPAPP 70 80 90 100 110 120 QPTPRKLVPI KPAPLPLSPC KNSFSILSSK GNILQIQGSQ LSTSYPGGQF VFAIQNPTLI 130 140 150 160 170 180 NKGSRSNASI QYQVPQIQGN SSQTIQVQPS LTNQIQVIPG TNQAITTPST SGHKPVPIKP 190 200 210 220 230 240 APVQKSSTTT TPVQSGANVV KLTGGGSNMT LTLPLNNLVN TSDIGGPAQL LTESPPTPLS 250 260 270 280 290 300 KTNKKARKKS LPVSQPSVAV AEQVETVLIE TTADNIIQAG NNLLIVQSPG GGQPAVVQQV 310 320 330 340 350 360 QVVPPKAEQQ QVVQIPQQAL RVVQAASATL PTVPQKPSQN FQIQTTEPTP TQVYIRTPSG 370 380 390 400 410 420 EVQTVLVQDS PPATAATTST VTCNSPALRA PHLSGTSKKH SAAILRKERP LPKIAPAGSI 430 440 450 460 470 480 ISLNAAQLAA AAQAMQTINI NGVQVQGVPV TITNTGGQQQ LTVQNVSGNN LTISGLSPTQ 490 500 510 520 530 540 IQLQMEQALA GEAQPGEKRR RMACTCPNCK DGEKRSGEQG KKKHVCHIPD CGKTFRKTSL 550 560 570 580 590 600 LRAHVRLHTG ERPFVCNWFF CGKRFTRSDE LQRHARTHTG DKRFECAQCQ KRFMRSDHLT 610 KHYKTHLGTK GL
	Isoform 2 [UniParc]. Checksum: 2E48ACE7A8EF1B70	FASTA	605	64,131
	10 20 30 40 50 60 MAATAAVSPS DYLQPAAATT QDSQPSPLAL LAATCSKIGP PAVEAAVTPP APPQPTPRKL 70 80 90 100 110 120 VPIKPAPLPL SPCKNSFSIL SSKGNILQIQ GSQLSTSYPG GQFVFAIQNP TLINKGSRSN 130 140 150 160 170 180 ASIQYQVPQI QGNSSQTIQV QPSLTNQIQV IPGTNQAITT PSTSGHKPVP IKPAPVQKSS 190 200 210 220 230 240 TTTTPVQSGA NVVKLTGGGS NMTLTLPLNN LVNTSDIGGP AQLLTESPPT PLSKTNKKAR 250 260 270 280 290 300 KKSLPVSQPS VAVAEQVETV LIETTADNII QAGNNLLIVQ SPGGGQPAVV QQVQVVPPKA 310 320 330 340 350 360 EQQQVVQIPQ QALRVVQAAS ATLPTVPQKP SQNFQIQTTE PTPTQVYIRT PSGEVQTVLV 370 380 390 400 410 420 QDSPPATAAT TSTVTCNSPA LRAPHLSGTS KKHSAAILRK ERPLPKIAPA GSIISLNAAQ 430 440 450 460 470 480 LAAAAQAMQT ININGVQVQG VPVTITNTGG QQQLTVQNVS GNNLTISGLS PTQIQLQMEQ 490 500 510 520 530 540 ALAGEAQPGE KRRRMACTCP NCKDGEKRSG EQGKKKHVCH IPDCGKTFRK TSLLRAHVRL 550 560 570 580 590 600 HTGERPFVCN WFFCGKRFTR SDELQRHART HTGDKRFECA QCQKRFMRSD HLTKHYKTHL GTKGL

References

[1]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Pancreatic islet.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Eye and Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK172877 Transcribed RNA. Translation: BAD32155.1. Different initiation. AK019649 mRNA. Translation: BAB31823.1. BC021759 mRNA. Translation: AAH21759.1. Different initiation. BC086457 mRNA. Translation: AAH86457.1. Different initiation.
IPI	IPI00816909. IPI00987370.
RefSeq	NP_001074433.1. NM_001080964.1. NP_084496.2. NM_030220.3.
UniGene	Mm.155547.
3D structure databases
HSSP	HSSP built from PDB template 1SP2 based on UniProtKB P08047.
ProteinModelPortal	Q9D2H6.
SMR	Q9D2H6. Positions 490-607.
ModBase	Search...
PTM databases
PhosphoSite	Q9D2H6.
Proteomic databases
PaxDb	Q9D2H6.
PRIDE	Q9D2H6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	78912.
KEGG	mmu:78912.
UCSC	uc007ldg.1. mouse.
Organism-specific databases
CTD	6668.
MGI	MGI:1926162. Sp2.
Rouge	Search...
Phylogenomic databases
eggNOG	COG5048.
HOVERGEN	HBG008933.
InParanoid	Q9D2H6.
KO	K09192.
Gene expression databases
CleanEx	MM_SP2.
Genevestigator	Q9D2H6.
Family and domain databases
Gene3D	3.30.160.60. 3 hits.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
SMART	SM00355. ZnF_C2H2. 3 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 3 hits. PS50157. ZINC_FINGER_C2H2_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	349734.
SOURCE	Search...

Entry information

Entry name

SP2_MOUSE

Accession

Primary (citable) accession number: Q9D2H6
Secondary accession number(s): Q6A0E1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program