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Protein

Testis-specific serine/threonine-protein kinase 3

Gene

Tssk3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May be involved in a signaling pathway during male germ cell development or mature sperm function.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by phosphorylation on Thr-168, potentially by autophosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391ATPPROSITE-ProRule annotation
Active sitei134 – 1341Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 249ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • intracellular signal transduction Source: UniProtKB
  • multicellular organism development Source: UniProtKB-KW
  • protein phosphorylation Source: UniProtKB
  • spermatogenesis Source: UniProtKB
  • sperm capacitation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific serine/threonine-protein kinase 3 (EC:2.7.11.1)
Short name:
TSK-3
Short name:
TSSK-3
Short name:
Testis-specific kinase 3
Alternative name(s):
Serine/threonine-protein kinase 22C
Gene namesi
Name:Tssk3
Synonyms:Stk22c, Stk22d
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1929914. Tssk3.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 268268Testis-specific serine/threonine-protein kinase 3PRO_0000086771Add
BLAST

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9D2E1.
PaxDbiQ9D2E1.
PRIDEiQ9D2E1.

PTM databases

iPTMnetiQ9D2E1.
PhosphoSiteiQ9D2E1.

Expressioni

Tissue specificityi

Developmentally expressed in testicular germ cells. In adult testis, expression was detected in round and condensing spermatids, but not in meiotic pachytene spermatocytes. Not expressed in brain, ovary, kidney, liver or early embryonic cells.1 Publication

Developmental stagei

Expression begins 20-24 days after birth and is maximal in the adult. The pattern of expression suggests that STK22D is expressed postmeiotically.

Gene expression databases

BgeeiQ9D2E1.
CleanExiMM_TSSK3.
GenevisibleiQ9D2E1. MM.

Interactioni

Protein-protein interaction databases

BioGridi208450. 2 interactions.
STRINGi10090.ENSMUSP00000000421.

Structurei

3D structure databases

ProteinModelPortaliQ9D2E1.
SMRiQ9D2E1. Positions 10-265.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 265256Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00770000120521.
HOGENOMiHOG000233016.
HOVERGENiHBG101110.
InParanoidiQ9D2E1.
KOiK08811.
OMAiHDSRKGD.
OrthoDBiEOG7P8P8F.
PhylomeDBiQ9D2E1.
TreeFamiTF352374.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9D2E1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDFLLSNGY QLGKTIGEGT YSKVKEAFSK KHQRKVAIKI IDKMGGPEEF
60 70 80 90 100
IQRFLPRELQ IVRTLDHKNI IQVYEMLESA DGKIYLVMEL AEGGDVFDCV
110 120 130 140 150
LNGGPLPESR AKALFRQMVE AIRYCHGCGV AHRDLKCENA LLQGFNLKLT
160 170 180 190 200
DFGFAKVLPK SRRELSQTFC GSTAYAAPEV LQGIPHDSKK GDVWSMGVVL
210 220 230 240 250
YVMLCASLPF DDTDIPKMLW QQQKGVSFPT HLGISTECQD LLKRLLEPDM
260
ILRPSIEEVS WHPWLAST
Length:268
Mass (Da):30,209
Last modified:June 1, 2001 - v1
Checksum:i4D4B8CE69BCA6705
GO

Sequence cautioni

The sequence AAF72581.1 differs from that shown. Reason: Frameshift at positions 110 and 131. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF298901 mRNA. Translation: AAK97209.1.
AF201734 mRNA. Translation: AAF72581.1. Frameshift.
AK019840 mRNA. Translation: BAB31876.1.
BC048470 mRNA. Translation: AAH48470.1.
CCDSiCCDS18693.1.
RefSeqiNP_536690.1. NM_080442.2.
UniGeneiMm.143802.

Genome annotation databases

EnsembliENSMUST00000000421; ENSMUSP00000000421; ENSMUSG00000000411.
GeneIDi58864.
KEGGimmu:58864.
UCSCiuc008uxc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF298901 mRNA. Translation: AAK97209.1.
AF201734 mRNA. Translation: AAF72581.1. Frameshift.
AK019840 mRNA. Translation: BAB31876.1.
BC048470 mRNA. Translation: AAH48470.1.
CCDSiCCDS18693.1.
RefSeqiNP_536690.1. NM_080442.2.
UniGeneiMm.143802.

3D structure databases

ProteinModelPortaliQ9D2E1.
SMRiQ9D2E1. Positions 10-265.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208450. 2 interactions.
STRINGi10090.ENSMUSP00000000421.

PTM databases

iPTMnetiQ9D2E1.
PhosphoSiteiQ9D2E1.

Proteomic databases

EPDiQ9D2E1.
PaxDbiQ9D2E1.
PRIDEiQ9D2E1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000421; ENSMUSP00000000421; ENSMUSG00000000411.
GeneIDi58864.
KEGGimmu:58864.
UCSCiuc008uxc.1. mouse.

Organism-specific databases

CTDi81629.
MGIiMGI:1929914. Tssk3.

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00770000120521.
HOGENOMiHOG000233016.
HOVERGENiHBG101110.
InParanoidiQ9D2E1.
KOiK08811.
OMAiHDSRKGD.
OrthoDBiEOG7P8P8F.
PhylomeDBiQ9D2E1.
TreeFamiTF352374.

Miscellaneous databases

PROiQ9D2E1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D2E1.
CleanExiMM_TSSK3.
GenevisibleiQ9D2E1. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and chromosomal localization of a gene encoding a novel serine/threonine kinase belonging to the subfamily of testis-specific kinases."
    Visconti P.E., Hao Z., Purdon M.A., Stein P., Balsara B.R., Testa J.R., Herr J.C., Moss S.B., Kopf G.S.
    Genomics 77:163-170(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Germ cell.
  2. "A novel member of the testis specific serine kinase family, tssk-3, expressed in the Leydig cells of sexually mature mice."
    Zuercher G., Rohrbach V., Andres A.-C., Ziemiecki A.
    Mech. Dev. 93:175-177(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Testis.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiTSSK3_MOUSE
AccessioniPrimary (citable) accession number: Q9D2E1
Secondary accession number(s): Q9JL98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: June 1, 2001
Last modified: June 8, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

PubMed:10781952 has termed the gene 'STK22D' as it was then thought that there were two different closely related genes.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.