Q9D287 (SPF27_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor SPF27

Alternative name(s):
Breast carcinoma-amplified sequence 2 homolog
DNA amplified in mammary carcinoma 1 protein

Gene names

Name:	Bcas2
Synonyms:	Dam1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in mRNA splicing By similarity.
Subunit structure	Associated with the spliceosome By similarity.
Subcellular location	Nucleus › nucleolus By similarity.
Sequence similarities	Belongs to the SPF27 family.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Domain	Coiled coil
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell spliceosomal complex Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 225	224	Pre-mRNA-splicing factor SPF27		PRO_0000064862
Regions
	Coiled coil	138 – 222	85	Potential
	Compositional bias	31 – 34	4	Poly-Ala
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
Experimental info
	Sequence conflict	28	1	V → A in BAB24258. Ref.2
	Sequence conflict	106	1	C → W in BAB24258. Ref.2
	Sequence conflict	137	1	V → G in BAB31409. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9D287 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 9112718EEFD96890	FASTA	225	26,131
	10 20 30 40 50 60 MAGTGLVAGE VVVDALPYFD QGYEAPGVRE AAAALVEEET RRYRPTKNYL SYLTAPDYSA 70 80 90 100 110 120 FETDIMRNEF ERLAARQPIE LLSMKRYELP APSSGQKNDI TAWQECVNNS MAQLEHQAVR 130 140 150 160 170 180 IENLELMSQH GCNAWKVYNE NLVHMIEHAQ KELQKLRKHI QDLNWQRKNM QLTAGSKLRE 190 200 210 220 MESNWVSLVS KNYEIERTIV QLENEIYQIK QQHGEANKEN IRQDF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse DAM1 regulates pro-apoptotic activity of BLK in mammary epithelial cells." Lee S., Ha S., Chung M., Kim Y., Choi Y. Cancer Lett. 188:121-126(2002) [PubMed: 12406557] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Cerebellum, Olfactory bulb and Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Colon.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY043239 mRNA. Translation: AAK85306.1. AK005825 mRNA. Translation: BAB24258.1. AK018786 mRNA. Translation: BAB31409.1. AK020098 mRNA. Translation: BAB31992.1. AK166967 mRNA. Translation: BAE39150.1. BC037062 mRNA. Translation: AAH37062.1.
IPI	IPI00316184.
RefSeq	NP_080878.2. NM_026602.3.
UniGene	Mm.104919.
3D structure databases
ProteinModelPortal	Q9D287.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9D287.
PTM databases
PhosphoSite	Q9D287.
Proteomic databases
PRIDE	Q9D287.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000005830; ENSMUSP00000005830; ENSMUSG00000005687.
GeneID	68183.
KEGG	mmu:68183.
UCSC	uc008qsu.1. mouse.
Organism-specific databases
CTD	10286.
MGI	MGI:1915433. Bcas2.
Phylogenomic databases
GeneTree	ENSGT00390000014494.
HOGENOM	HBG590081.
HOVERGEN	HBG050675.
InParanoid	Q9D287.
OMA	TEIMRNE.
OrthoDB	EOG4BK54T.
Gene expression databases
ArrayExpress	Q9D287.
Bgee	Q9D287.
CleanEx	MM_BCAS2.
Genevestigator	Q9D287.
GermOnline	ENSMUSG00000005687. Mus musculus.
Family and domain databases
InterPro	IPR008409. BCAS2. [Graphical view]
KO	K12861.
Pfam	PF05700. BCAS2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	326630.
SOURCE	Search...

Entry information

Entry name

SPF27_MOUSE

Accession

Primary (citable) accession number: Q9D287
Secondary accession number(s): Q3TKJ5 , Q91YX8, Q9D2U4, Q9DAI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	June 1, 2001
Last modified:	November 16, 2011
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program