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Protein

Pre-mRNA-splicing factor SPF27

Gene

Bcas2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor SPF27
Alternative name(s):
Breast carcinoma-amplified sequence 2 homolog
DNA amplified in mammary carcinoma 1 protein
Gene namesi
Name:Bcas2
Synonyms:Dam1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1915433. Bcas2.

Subcellular locationi

  • Nucleusnucleolus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 225224Pre-mRNA-splicing factor SPF27PRO_0000064862Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9D287.
MaxQBiQ9D287.
PaxDbiQ9D287.
PRIDEiQ9D287.

PTM databases

PhosphoSiteiQ9D287.

Expressioni

Gene expression databases

BgeeiQ9D287.
CleanExiMM_BCAS2.
ExpressionAtlasiQ9D287. baseline and differential.
GenevisibleiQ9D287. MM.

Interactioni

Subunit structurei

Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts directly in the complex with PRPF19, CDC5L and PLRG1.By similarity

Protein-protein interaction databases

BioGridi212708. 34 interactions.
IntActiQ9D287. 35 interactions.
MINTiMINT-4126699.
STRINGi10090.ENSMUSP00000005830.

Structurei

3D structure databases

ProteinModelPortaliQ9D287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili138 – 22285Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi31 – 344Poly-Ala

Sequence similaritiesi

Belongs to the SPF27 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3096. Eukaryota.
ENOG41101IB. LUCA.
GeneTreeiENSGT00390000014494.
HOGENOMiHOG000007540.
HOVERGENiHBG050675.
InParanoidiQ9D287.
KOiK12861.
OMAiQNRLPME.
OrthoDBiEOG7RBZ99.
PhylomeDBiQ9D287.
TreeFamiTF105818.

Family and domain databases

InterProiIPR008409. SPF27.
[Graphical view]
PANTHERiPTHR13296:SF0. PTHR13296:SF0. 1 hit.
PfamiPF05700. BCAS2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D287-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGTGLVAGE VVVDALPYFD QGYEAPGVRE AAAALVEEET RRYRPTKNYL
60 70 80 90 100
SYLTAPDYSA FETDIMRNEF ERLAARQPIE LLSMKRYELP APSSGQKNDI
110 120 130 140 150
TAWQECVNNS MAQLEHQAVR IENLELMSQH GCNAWKVYNE NLVHMIEHAQ
160 170 180 190 200
KELQKLRKHI QDLNWQRKNM QLTAGSKLRE MESNWVSLVS KNYEIERTIV
210 220
QLENEIYQIK QQHGEANKEN IRQDF
Length:225
Mass (Da):26,131
Last modified:June 1, 2001 - v1
Checksum:i9112718EEFD96890
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281V → A in BAB24258 (PubMed:16141072).Curated
Sequence conflicti106 – 1061C → W in BAB24258 (PubMed:16141072).Curated
Sequence conflicti137 – 1371V → G in BAB31409 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY043239 mRNA. Translation: AAK85306.1.
AK005825 mRNA. Translation: BAB24258.1.
AK018786 mRNA. Translation: BAB31409.1.
AK020098 mRNA. Translation: BAB31992.1.
AK166967 mRNA. Translation: BAE39150.1.
BC037062 mRNA. Translation: AAH37062.1.
CCDSiCCDS17691.1.
RefSeqiNP_080878.2. NM_026602.3.
UniGeneiMm.104919.

Genome annotation databases

EnsembliENSMUST00000005830; ENSMUSP00000005830; ENSMUSG00000005687.
GeneIDi68183.
KEGGimmu:68183.
UCSCiuc008qsu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY043239 mRNA. Translation: AAK85306.1.
AK005825 mRNA. Translation: BAB24258.1.
AK018786 mRNA. Translation: BAB31409.1.
AK020098 mRNA. Translation: BAB31992.1.
AK166967 mRNA. Translation: BAE39150.1.
BC037062 mRNA. Translation: AAH37062.1.
CCDSiCCDS17691.1.
RefSeqiNP_080878.2. NM_026602.3.
UniGeneiMm.104919.

3D structure databases

ProteinModelPortaliQ9D287.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212708. 34 interactions.
IntActiQ9D287. 35 interactions.
MINTiMINT-4126699.
STRINGi10090.ENSMUSP00000005830.

PTM databases

PhosphoSiteiQ9D287.

Proteomic databases

EPDiQ9D287.
MaxQBiQ9D287.
PaxDbiQ9D287.
PRIDEiQ9D287.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005830; ENSMUSP00000005830; ENSMUSG00000005687.
GeneIDi68183.
KEGGimmu:68183.
UCSCiuc008qsu.1. mouse.

Organism-specific databases

CTDi10286.
MGIiMGI:1915433. Bcas2.

Phylogenomic databases

eggNOGiKOG3096. Eukaryota.
ENOG41101IB. LUCA.
GeneTreeiENSGT00390000014494.
HOGENOMiHOG000007540.
HOVERGENiHBG050675.
InParanoidiQ9D287.
KOiK12861.
OMAiQNRLPME.
OrthoDBiEOG7RBZ99.
PhylomeDBiQ9D287.
TreeFamiTF105818.

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSiBcas2. mouse.
PROiQ9D287.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D287.
CleanExiMM_BCAS2.
ExpressionAtlasiQ9D287. baseline and differential.
GenevisibleiQ9D287. MM.

Family and domain databases

InterProiIPR008409. SPF27.
[Graphical view]
PANTHERiPTHR13296:SF0. PTHR13296:SF0. 1 hit.
PfamiPF05700. BCAS2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse DAM1 regulates pro-apoptotic activity of BLK in mammary epithelial cells."
    Lee S., Ha S., Chung M., Kim Y., Choi Y.
    Cancer Lett. 188:121-126(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum, Olfactory bulb and Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Kidney, Lung, Spleen and Testis.

Entry informationi

Entry nameiSPF27_MOUSE
AccessioniPrimary (citable) accession number: Q9D287
Secondary accession number(s): Q3TKJ5
, Q91YX8, Q9D2U4, Q9DAI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2001
Last modified: June 8, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.