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Protein

Ribonuclease K6

Gene

Rnase6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Pyrimidine specific nuclease with a preference for U.By similarity

Kineticsi

  1. KM=2.1 µM for tRNA1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei41 – 411Proton acceptorBy similarity
Binding sitei89 – 891SubstrateBy similarity
Active sitei148 – 1481Proton donorBy similarity

GO - Molecular functioni

  1. endonuclease activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease K6 (EC:3.1.27.-)
Short name:
RNase K6
Gene namesi
Name:Rnase6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1925666. Rnase6.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Chaini28 – 153126Ribonuclease K6PRO_0000030900Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi49 ↔ 107By similarity
Glycosylationi58 – 581N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi63 ↔ 117By similarity
Disulfide bondi81 ↔ 132By similarity
Glycosylationi85 – 851N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi88 ↔ 95By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ9D244.

Expressioni

Tissue specificityi

Expression detected in kidney, skeletal muscle, heart, bone marrow and spleen.1 Publication

Gene expression databases

CleanExiMM_RNASE6.
GenevestigatoriQ9D244.

Structurei

3D structure databases

ProteinModelPortaliQ9D244.
SMRiQ9D244. Positions 27-153.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni64 – 685Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG45773.
HOGENOMiHOG000276882.
HOVERGENiHBG008396.
InParanoidiQ9D244.
KOiK01172.
PhylomeDBiQ9D244.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D244-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVVDLPRYLP LLLLLELWEP MYLLCSQPKG LSRAHWFEIQ HIQTSRQPCN
60 70 80 90 100
TAMRGVNNYT QHCKQINTFL HESFQNVAAT CSLHNITCKN GRKNCHESAE
110 120 130 140 150
PVKMTDCSHT GGAYPNCRYS SDKQYKFFIV ACEHPKKEDP PYQLVPVHLD

KIV
Length:153
Mass (Da):17,675
Last modified:July 5, 2005 - v2
Checksum:i84A6C788AEE7CA05
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti42 – 421I → V in BAB32143. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY545655 mRNA. Translation: AAS48640.1.
AK020595 mRNA. Translation: BAB32143.1.
BC094892 mRNA. Translation: AAH94892.1.
BC132632 mRNA. Translation: AAI32633.1.
CCDSiCCDS27037.1.
RefSeqiNP_084374.2. NM_030098.2.
XP_006519761.1. XM_006519698.1.
UniGeneiMm.103835.

Genome annotation databases

GeneIDi78416.
KEGGimmu:78416.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY545655 mRNA. Translation: AAS48640.1.
AK020595 mRNA. Translation: BAB32143.1.
BC094892 mRNA. Translation: AAH94892.1.
BC132632 mRNA. Translation: AAI32633.1.
CCDSiCCDS27037.1.
RefSeqiNP_084374.2. NM_030098.2.
XP_006519761.1. XM_006519698.1.
UniGeneiMm.103835.

3D structure databases

ProteinModelPortaliQ9D244.
SMRiQ9D244. Positions 27-153.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ9D244.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi78416.
KEGGimmu:78416.

Organism-specific databases

CTDi6039.
MGIiMGI:1925666. Rnase6.

Phylogenomic databases

eggNOGiNOG45773.
HOGENOMiHOG000276882.
HOVERGENiHBG008396.
InParanoidiQ9D244.
KOiK01172.
PhylomeDBiQ9D244.

Miscellaneous databases

NextBioi348836.
PROiQ9D244.
SOURCEiSearch...

Gene expression databases

CleanExiMM_RNASE6.
GenevestigatoriQ9D244.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, characterization, and evolutionary divergence of mouse RNase 6: evidence for unusual evolution in rodents."
    Dyer K.D., Rosenberg H.F., Zhang J.
    J. Mol. Evol. 59:657-665(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Urinary bladder.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Pancreas.

Entry informationi

Entry nameiRNAS6_MOUSE
AccessioniPrimary (citable) accession number: Q9D244
Secondary accession number(s): A2RTT6, Q6QDX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 19, 2001
Last sequence update: July 5, 2005
Last modified: February 4, 2015
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.