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Protein

Ribonuclease K6

Gene

Rnase6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pyrimidine specific nuclease with a preference for U.By similarity

Kineticsi

  1. KM=2.1 µM for tRNA1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei41Proton acceptorBy similarity1
    Binding sitei89SubstrateBy similarity1
    Active sitei148Proton donorBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    • antibacterial humoral response Source: UniProtKB
    • antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
    • defense response to Gram-negative bacterium Source: UniProtKB
    • defense response to Gram-positive bacterium Source: UniProtKB
    • innate immune response Source: UniProtKB
    • RNA phosphodiester bond hydrolysis Source: MGI

    Keywordsi

    Molecular functionEndonuclease, Hydrolase, Nuclease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease K6 (EC:3.1.27.-)
    Short name:
    RNase K6
    Gene namesi
    Name:Rnase6
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Unplaced

    Organism-specific databases

    MGIiMGI:1925666. Rnase6.

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 27Sequence analysisAdd BLAST27
    ChainiPRO_000003090028 – 153Ribonuclease K6Add BLAST126

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi49 ↔ 107By similarity
    Glycosylationi58N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi63 ↔ 117By similarity
    Disulfide bondi81 ↔ 132By similarity
    Glycosylationi85N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi88 ↔ 95By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ9D244.
    PRIDEiQ9D244.

    Expressioni

    Tissue specificityi

    Expression detected in kidney, skeletal muscle, heart, bone marrow and spleen.1 Publication

    Gene expression databases

    CleanExiMM_RNASE6.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000093613.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9D244.
    SMRiQ9D244.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni64 – 68Substrate bindingBy similarity5

    Sequence similaritiesi

    Belongs to the pancreatic ribonuclease family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG410IYEW. Eukaryota.
    ENOG4111D8Q. LUCA.
    HOGENOMiHOG000276882.
    HOVERGENiHBG008396.
    InParanoidiQ9D244.
    KOiK01172.
    PhylomeDBiQ9D244.

    Family and domain databases

    Gene3Di3.10.130.10. 1 hit.
    InterProiView protein in InterPro
    IPR001427. RNaseA.
    IPR023411. RNaseA_AS.
    IPR023412. RNaseA_domain.
    PANTHERiPTHR11437. PTHR11437. 1 hit.
    PfamiView protein in Pfam
    PF00074. RnaseA. 1 hit.
    PRINTSiPR00794. RIBONUCLEASE.
    ProDomiView protein in ProDom or Entries sharing at least one domain
    PD000535. RNaseA. 1 hit.
    SMARTiView protein in SMART
    SM00092. RNAse_Pc. 1 hit.
    SUPFAMiSSF54076. SSF54076. 1 hit.
    PROSITEiView protein in PROSITE
    PS00127. RNASE_PANCREATIC. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9D244-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVVDLPRYLP LLLLLELWEP MYLLCSQPKG LSRAHWFEIQ HIQTSRQPCN
    60 70 80 90 100
    TAMRGVNNYT QHCKQINTFL HESFQNVAAT CSLHNITCKN GRKNCHESAE
    110 120 130 140 150
    PVKMTDCSHT GGAYPNCRYS SDKQYKFFIV ACEHPKKEDP PYQLVPVHLD

    KIV
    Length:153
    Mass (Da):17,675
    Last modified:July 5, 2005 - v2
    Checksum:i84A6C788AEE7CA05
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti42I → V in BAB32143 (PubMed:16141072).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY545655 mRNA. Translation: AAS48640.1.
    AK020595 mRNA. Translation: BAB32143.1.
    BC094892 mRNA. Translation: AAH94892.1.
    BC132632 mRNA. Translation: AAI32633.1.
    CCDSiCCDS27037.1.
    RefSeqiNP_084374.2. NM_030098.2.
    XP_006519761.1. XM_006519698.2.
    UniGeneiMm.103835.

    Genome annotation databases

    GeneIDi78416.
    KEGGimmu:78416.

    Similar proteinsi

    Entry informationi

    Entry nameiRNAS6_MOUSE
    AccessioniPrimary (citable) accession number: Q9D244
    Secondary accession number(s): A2RTT6, Q6QDX5
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 19, 2001
    Last sequence update: July 5, 2005
    Last modified: September 27, 2017
    This is version 107 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families