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Protein

Endoplasmic reticulum resident protein 44

Gene

Erp44

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif. Inhibits the calcium channel activity of ITPR1. May have a role in the control of oxidative protein folding in the endoplasmic reticulum. Required to retain ERO1A and ERO1B in the endoplasmic reticulum (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum resident protein 44
Short name:
ER protein 44
Short name:
ERp44
Alternative name(s):
Thioredoxin domain-containing protein 4
Gene namesi
Name:Erp44
Synonyms:Kiaa0573, Txndc4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1923549 Erp44

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi58C → S: No effect on interaction with ITPR1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29By similarityAdd BLAST29
ChainiPRO_000003418130 – 406Endoplasmic reticulum resident protein 44Add BLAST377

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi189 ↔ 241By similarity
Disulfide bondi301 ↔ 318By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiQ9D1Q6
MaxQBiQ9D1Q6
PaxDbiQ9D1Q6
PeptideAtlasiQ9D1Q6
PRIDEiQ9D1Q6

2D gel databases

REPRODUCTION-2DPAGEiIPI00134058
Q9D1Q6

PTM databases

iPTMnetiQ9D1Q6
PhosphoSitePlusiQ9D1Q6
SwissPalmiQ9D1Q6

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000028343
GenevisibleiQ9D1Q6 MM

Interactioni

Subunit structurei

Forms mixed disulfides with both ERO1A and ERO1B and cargo folding intermediates (By similarity). Directly interacts with ITPR1 in a pH-, redox state- and calcium-dependent manner, but not with ITPR2 or ITPR3. The strength of this interaction inversely correlates with calcium concentration.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Itpr1P118815EBI-541567,EBI-541478

Protein-protein interaction databases

BioGridi218067, 12 interactors
IntActiQ9D1Q6, 21 interactors
MINTiQ9D1Q6
STRINGi10090.ENSMUSP00000030028

Structurei

3D structure databases

ProteinModelPortaliQ9D1Q6
SMRiQ9D1Q6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 138ThioredoxinPROSITE-ProRule annotationAdd BLAST109

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni236 – 285Interaction with ITPR11 PublicationAdd BLAST50

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi403 – 406Prevents secretion from ERPROSITE-ProRule annotation4

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0912 Eukaryota
COG0526 LUCA
GeneTreeiENSGT00860000133691
HOGENOMiHOG000007707
InParanoidiQ9D1Q6
KOiK17264
OMAiKLGPCVA
OrthoDBiEOG091G08L8
PhylomeDBiQ9D1Q6
TreeFamiTF106378

Family and domain databases

InterProiView protein in InterPro
IPR036249 Thioredoxin-like_sf
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF00085 Thioredoxin, 1 hit
SUPFAMiSSF52833 SSF52833, 3 hits
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS51352 THIOREDOXIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D1Q6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPAVFLSLA DLRCSLLLLV TSIFTPITAE IASLDSENID EILNNADVAL
60 70 80 90 100
VNFYADWCRF SQMLHPIFEE ASDVIKEEYP DKNQVVFARV DCDQHSDIAQ
110 120 130 140 150
RYRISKYPTL KLFRNGMMMK REYRGQRSVK ALADYIRQQK SNPVHEIQSL
160 170 180 190 200
DEVTNLDRSK RNIIGYFEQK DSENYRVFER VASILHDDCA FLSAFGDLSK
210 220 230 240 250
PERYNGDNVI YKPPGRSAPD MVYLGSMTNF DVTYNWIQDK CVPLVREITF
260 270 280 290 300
ENGEELTEEG LPFLILFHMK DDTESLEIFQ NEVARQLISE KGTINFLHAD
310 320 330 340 350
CDKFRHPLLH IQKTPADCPV IAIDSFRHMY VFGDFKDVLI PGKLKQFVFD
360 370 380 390 400
LHSGKLHREF HHGPDPTDTA PGEQDQDVAS SPPESSFQKL APSEYRYTLL

RDRDEL
Length:406
Mass (Da):46,853
Last modified:June 1, 2001 - v1
Checksum:iF87935A5EB932927
GO

Sequence cautioni

The sequence BAD32251 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003217 mRNA Translation: BAB22648.1
AK151497 mRNA Translation: BAE30449.1
AK160113 mRNA Translation: BAE35637.1
AK172973 mRNA Translation: BAD32251.1 Different initiation.
BC019558 mRNA Translation: AAH19558.1
CCDSiCCDS18165.1
RefSeqiNP_083848.1, NM_029572.2
UniGeneiMm.317701

Genome annotation databases

EnsembliENSMUST00000030028; ENSMUSP00000030028; ENSMUSG00000028343
GeneIDi76299
KEGGimmu:76299
UCSCiuc008suy.1 mouse

Similar proteinsi

Entry informationi

Entry nameiERP44_MOUSE
AccessioniPrimary (citable) accession number: Q9D1Q6
Secondary accession number(s): Q3TVI1, Q6A045
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health