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Protein

Histone acetyltransferase KAT8

Gene

Kat8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Histone acetyltransferase which may be involved in transcriptional activation. May influence the function of ATM. As part of the MSL complex it is involved in acetylation of nucleosomal histone H4 producing specifically H4K16ac. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. That activity is less specific than the one of the MSL complex. Can also acetylate TP53/p53 at 'Lys-120'.By similarity

Catalytic activityi

Acetyl-CoA + [histone] = CoA + acetyl-[histone].By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei350Proton donor/acceptorBy similarity1
Binding sitei354Acetyl-CoABy similarity1
Binding sitei363Acetyl-CoABy similarity1
Binding sitei432Acetyl-CoABy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri207 – 232C2HC MYST-typePROSITE-ProRule annotationAdd BLAST26

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Acyltransferase, Chromatin regulator, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-3214847. HATs acetylate histones.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone acetyltransferase KAT8 (EC:2.3.1.48By similarity)
Alternative name(s):
Lysine acetyltransferase 8
MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1
Short name:
MYST-1
Gene namesi
Name:Kat8
Synonyms:Mof, Myst1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1915023. Kat8.

Subcellular locationi

GO - Cellular componenti

  • histone acetyltransferase complex Source: UniProtKB
  • kinetochore Source: MGI
  • MLL1 complex Source: UniProtKB
  • MSL complex Source: UniProtKB
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000515672 – 458Histone acetyltransferase KAT8Add BLAST457

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei37PhosphoserineBy similarity1
Modified residuei42PhosphoserineBy similarity1
Modified residuei113N6-acetyllysineBy similarity1
Modified residuei274N6-acetyllysine; by autocatalysisBy similarity1
Modified residuei348PhosphoserineBy similarity1

Post-translational modificationi

Autoacetylation at Lys-274 is required for binding histone H4 with high affinity and for proper function.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9D1P2.
PaxDbiQ9D1P2.
PeptideAtlasiQ9D1P2.
PRIDEiQ9D1P2.

PTM databases

iPTMnetiQ9D1P2.
PhosphoSitePlusiQ9D1P2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030801.
CleanExiMM_MYST1.
ExpressionAtlasiQ9D1P2. baseline and differential.
GenevisibleiQ9D1P2. MM.

Interactioni

Subunit structurei

Component of a multisubunit histone acetyltransferase complex (MSL) at least composed of the MOF/KAT8, MSL1/hampin, MSL2L1 and MSL3L1. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Interacts with KANSL1; the interaction is direct. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MOF/KAT8, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with the chromodomain of MORF4L1/MRG15. Interacts with ATM through the chromodomain (By similarity). Interacts with MSL1; the interaction is direct.By similarity1 Publication

GO - Molecular functioni

  • enzyme binding Source: UniProtKB
  • methylated histone binding Source: MGI
  • transcription factor binding Source: MGI

Protein-protein interaction databases

BioGridi212433. 35 interactors.
IntActiQ9D1P2. 32 interactors.
STRINGi10090.ENSMUSP00000033070.

Structurei

Secondary structure

1458
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi59 – 65Combined sources7
Turni66 – 68Combined sources3
Beta strandi69 – 81Combined sources13
Turni82 – 85Combined sources4
Beta strandi86 – 92Combined sources7
Turni94 – 96Combined sources3
Beta strandi102 – 104Combined sources3
Turni106 – 108Combined sources3
Turni112 – 114Combined sources3
Beta strandi115 – 117Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WGSNMR-A50-169[»]
ProteinModelPortaliQ9D1P2.
SMRiQ9D1P2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9D1P2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini69 – 123ChromoAdd BLAST55
Domaini174 – 447MYST-type HATPROSITE-ProRule annotationAdd BLAST274

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni174 – 458Sufficient for interaction with KANSL1By similarityAdd BLAST285
Regioni317 – 319Acetyl-CoA bindingBy similarity3
Regioni324 – 330Acetyl-CoA bindingBy similarity7

Sequence similaritiesi

Belongs to the MYST (SAS/MOZ) family.Curated
Contains 1 C2HC MYST-type zinc finger.PROSITE-ProRule annotation
Contains 1 chromo domain.Curated
Contains 1 MYST-type HAT (histone acetyltransferase) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri207 – 232C2HC MYST-typePROSITE-ProRule annotationAdd BLAST26

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2747. Eukaryota.
COG5027. LUCA.
GeneTreeiENSGT00550000074503.
HOGENOMiHOG000182457.
HOVERGENiHBG053268.
InParanoidiQ9D1P2.
KOiK11308.
OMAiMCLKWAP.
OrthoDBiEOG091G0B73.
PhylomeDBiQ9D1P2.
TreeFamiTF317619.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000953. Chromo/shadow_dom.
IPR016197. Chromodomain-like.
IPR002717. HAT_MYST-type.
IPR025995. Tudor-knot.
[Graphical view]
PfamiPF01853. MOZ_SAS. 1 hit.
PF11717. Tudor-knot. 1 hit.
[Graphical view]
SMARTiSM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMiSSF54160. SSF54160. 1 hit.
SSF55729. SSF55729. 1 hit.
PROSITEiPS51726. MYST_HAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D1P2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAQGATAAV AATTSGTVGE GEPGPGENAA VEGPARSPGR VSPPTPARGE
60 70 80 90 100
PEVTVEIGET YLCRRPDSTW HSAEVIQSRV NDQEGREEFY VHYVGFNRRL
110 120 130 140 150
DEWVDKNRLA LTKTVKDAVQ KNSEKYLSEL AEQPERKITR NQKRKHDEIN
160 170 180 190 200
HVQKTYAEMD PTTAALEKEH EAITKVKYVD KIHIGNYEID AWYFSPFPED
210 220 230 240 250
YGKQPKLWLC EYCLKYMKFE KSYRFHLGQC QWRQPPGKEI YRKSNISVYE
260 270 280 290 300
VDGKDHKIYC QNLCLLAKLF LDHKTLYFDV EPFVFYILTE VDRQGAHIVG
310 320 330 340 350
YFSKEKESPD GNNVACILTL PPYQRRGYGK FLIAFSYELS KLESTVGSPE
360 370 380 390 400
KPLSDLGKLS YRSYWSWVLL EILRDFRGTL SIKDLSQMTS ITQNDIISTL
410 420 430 440 450
QSLNMVKYWK GQHVICVTPK LVEEHLKSAQ YKKPPITVDS VCLKWAPPKH

KQVKLSKK
Length:458
Mass (Da):52,574
Last modified:June 1, 2001 - v1
Checksum:iFA25E0C8D7E46CEC
GO
Isoform 2 (identifier: Q9D1P2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     155-194: TYAEMDPTTA...GNYEIDAWYF → VLAPPFSGPS...LFTYEKILSL
     195-458: Missing.

Show »
Length:194
Mass (Da):21,398
Checksum:i36334369729A61C2
GO

Sequence cautioni

The sequence BAC25539 differs from that shown. Reason: Frameshift at position 236.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti385L → F in AAH36284 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014580155 – 194TYAEM…DAWYF → VLAPPFSGPSQQSHSNSHVK AGSMPGFFSRLFTYEKILSL in isoform 2. 1 PublicationAdd BLAST40
Alternative sequenceiVSP_014581195 – 458Missing in isoform 2. 1 PublicationAdd BLAST264

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003264 mRNA. Translation: BAB22680.1.
AK018002 mRNA. Translation: BAC25539.1. Frameshift.
AK029350 mRNA. Translation: BAC26411.1.
AK146710 mRNA. Translation: BAE27376.1.
BC036284 mRNA. Translation: AAH36284.1.
CCDSiCCDS21885.1. [Q9D1P2-1]
RefSeqiNP_080646.1. NM_026370.1. [Q9D1P2-1]
UniGeneiMm.425492.

Genome annotation databases

EnsembliENSMUST00000033070; ENSMUSP00000033070; ENSMUSG00000030801. [Q9D1P2-1]
GeneIDi67773.
KEGGimmu:67773.
UCSCiuc009jxg.1. mouse. [Q9D1P2-2]
uc009jxh.1. mouse. [Q9D1P2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003264 mRNA. Translation: BAB22680.1.
AK018002 mRNA. Translation: BAC25539.1. Frameshift.
AK029350 mRNA. Translation: BAC26411.1.
AK146710 mRNA. Translation: BAE27376.1.
BC036284 mRNA. Translation: AAH36284.1.
CCDSiCCDS21885.1. [Q9D1P2-1]
RefSeqiNP_080646.1. NM_026370.1. [Q9D1P2-1]
UniGeneiMm.425492.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WGSNMR-A50-169[»]
ProteinModelPortaliQ9D1P2.
SMRiQ9D1P2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212433. 35 interactors.
IntActiQ9D1P2. 32 interactors.
STRINGi10090.ENSMUSP00000033070.

PTM databases

iPTMnetiQ9D1P2.
PhosphoSitePlusiQ9D1P2.

Proteomic databases

EPDiQ9D1P2.
PaxDbiQ9D1P2.
PeptideAtlasiQ9D1P2.
PRIDEiQ9D1P2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033070; ENSMUSP00000033070; ENSMUSG00000030801. [Q9D1P2-1]
GeneIDi67773.
KEGGimmu:67773.
UCSCiuc009jxg.1. mouse. [Q9D1P2-2]
uc009jxh.1. mouse. [Q9D1P2-1]

Organism-specific databases

CTDi84148.
MGIiMGI:1915023. Kat8.

Phylogenomic databases

eggNOGiKOG2747. Eukaryota.
COG5027. LUCA.
GeneTreeiENSGT00550000074503.
HOGENOMiHOG000182457.
HOVERGENiHBG053268.
InParanoidiQ9D1P2.
KOiK11308.
OMAiMCLKWAP.
OrthoDBiEOG091G0B73.
PhylomeDBiQ9D1P2.
TreeFamiTF317619.

Enzyme and pathway databases

ReactomeiR-MMU-3214847. HATs acetylate histones.

Miscellaneous databases

EvolutionaryTraceiQ9D1P2.
PROiQ9D1P2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030801.
CleanExiMM_MYST1.
ExpressionAtlasiQ9D1P2. baseline and differential.
GenevisibleiQ9D1P2. MM.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000953. Chromo/shadow_dom.
IPR016197. Chromodomain-like.
IPR002717. HAT_MYST-type.
IPR025995. Tudor-knot.
[Graphical view]
PfamiPF01853. MOZ_SAS. 1 hit.
PF11717. Tudor-knot. 1 hit.
[Graphical view]
SMARTiSM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMiSSF54160. SSF54160. 1 hit.
SSF55729. SSF55729. 1 hit.
PROSITEiPS51726. MYST_HAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAT8_MOUSE
AccessioniPrimary (citable) accession number: Q9D1P2
Secondary accession number(s): Q3UIY0
, Q8BJ69, Q8BJ76, Q8CI73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.