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Protein

Adaptin ear-binding coat-associated protein 2

Gene

Necap2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in endocytosis.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Adaptin ear-binding coat-associated protein 2
Alternative name(s):
NECAP endocytosis-associated protein 2
Short name:
NECAP-2
Gene namesi
Name:Necap2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1913397. Necap2.

Subcellular locationi

GO - Cellular componenti

  • clathrin vesicle coat Source: UniProtKB
  • intracellular Source: MGI
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 266266Adaptin ear-binding coat-associated protein 2PRO_0000213072Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei181 – 1811PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9D1J1.
MaxQBiQ9D1J1.
PaxDbiQ9D1J1.
PRIDEiQ9D1J1.

PTM databases

iPTMnetiQ9D1J1.
PhosphoSiteiQ9D1J1.

Expressioni

Tissue specificityi

Expressed in brain, heart, kidney, liver, lung, skeletal muscles and testis (at protein level).

Gene expression databases

BgeeiQ9D1J1.
CleanExiMM_NECAP2.
ExpressionAtlasiQ9D1J1. baseline and differential.
GenevisibleiQ9D1J1. MM.

Interactioni

Subunit structurei

Interacts with AP1G1 and AP2A1 components of the adapter protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins GGA1, GGA2 and GGA3.1 Publication

Protein-protein interaction databases

IntActiQ9D1J1. 7 interactions.
MINTiMINT-4126596.
STRINGi10090.ENSMUSP00000030760.

Structurei

3D structure databases

ProteinModelPortaliQ9D1J1.
SMRiQ9D1J1. Positions 3-132.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi243 – 2464WXXF motif 1
Motifi263 – 2664WXXF motif 2

Domaini

The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself (By similarity).By similarity

Sequence similaritiesi

Belongs to the NECAP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2500. Eukaryota.
ENOG41113PS. LUCA.
GeneTreeiENSGT00390000009359.
HOGENOMiHOG000231188.
HOVERGENiHBG060621.
InParanoidiQ9D1J1.
OMAiIQFEDAT.
OrthoDBiEOG76X61J.
PhylomeDBiQ9D1J1.
TreeFamiTF314482.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR012466. NECAP-1_N.
IPR011993. PH_dom-like.
[Graphical view]
PfamiPF07933. DUF1681. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9D1J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEESEYESVL CVKPEVHVYR IPPRATNRGY RASEWQLDQP SWSGRLRITA
60 70 80 90 100
KGKVAYIKLE DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG
110 120 130 140 150
RRAFIGLGFG DRGDAFDFNV ALQDHFKWVK QQCEFAKQAQ NPDEGPKLDL
160 170 180 190 200
GFKDGQTIKI NIANMRKKEG AAGTPRARPT SAGGLSLLPP PPGGKSSTVI
210 220 230 240 250
PPSGEQLSVG GSLVQPAVVS GSGGATELWP QSKPAAAATA DIWGDFTKST
260
GSPSSQSQPG TGWVQF
Length:266
Mass (Da):28,598
Last modified:June 1, 2001 - v1
Checksum:i8687FFFF9DD901BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BK000657 mRNA. Translation: DAA01434.1.
AK003463 mRNA. Translation: BAB22803.1.
AK009714 mRNA. Translation: BAB26458.1.
AK155871 mRNA. Translation: BAE33474.1.
BC037069 mRNA. Translation: AAH37069.1.
CCDSiCCDS18862.1.
RefSeqiNP_079659.1. NM_025383.3.
UniGeneiMm.21187.

Genome annotation databases

EnsembliENSMUST00000030760; ENSMUSP00000030760; ENSMUSG00000028923.
GeneIDi66147.
KEGGimmu:66147.
UCSCiuc008vnu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BK000657 mRNA. Translation: DAA01434.1.
AK003463 mRNA. Translation: BAB22803.1.
AK009714 mRNA. Translation: BAB26458.1.
AK155871 mRNA. Translation: BAE33474.1.
BC037069 mRNA. Translation: AAH37069.1.
CCDSiCCDS18862.1.
RefSeqiNP_079659.1. NM_025383.3.
UniGeneiMm.21187.

3D structure databases

ProteinModelPortaliQ9D1J1.
SMRiQ9D1J1. Positions 3-132.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9D1J1. 7 interactions.
MINTiMINT-4126596.
STRINGi10090.ENSMUSP00000030760.

PTM databases

iPTMnetiQ9D1J1.
PhosphoSiteiQ9D1J1.

Proteomic databases

EPDiQ9D1J1.
MaxQBiQ9D1J1.
PaxDbiQ9D1J1.
PRIDEiQ9D1J1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030760; ENSMUSP00000030760; ENSMUSG00000028923.
GeneIDi66147.
KEGGimmu:66147.
UCSCiuc008vnu.2. mouse.

Organism-specific databases

CTDi55707.
MGIiMGI:1913397. Necap2.

Phylogenomic databases

eggNOGiKOG2500. Eukaryota.
ENOG41113PS. LUCA.
GeneTreeiENSGT00390000009359.
HOGENOMiHOG000231188.
HOVERGENiHBG060621.
InParanoidiQ9D1J1.
OMAiIQFEDAT.
OrthoDBiEOG76X61J.
PhylomeDBiQ9D1J1.
TreeFamiTF314482.

Miscellaneous databases

ChiTaRSiNecap2. mouse.
NextBioi320772.
PROiQ9D1J1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D1J1.
CleanExiMM_NECAP2.
ExpressionAtlasiQ9D1J1. baseline and differential.
GenevisibleiQ9D1J1. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR012466. NECAP-1_N.
IPR011993. PH_dom-like.
[Graphical view]
PfamiPF07933. DUF1681. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a family of endocytic proteins that define a new alpha-adaptin ear-binding motif."
    Ritter B., Philie J., Girard M., Tung E.C., Blondeau F., McPherson P.S.
    EMBO Rep. 4:1089-1095(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
    Strain: C57BL/6J.
    Tissue: Embryo.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo and Tongue.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Mammary tumor.
  4. "Definition of the consensus motif recognized by gamma-adaptin ear domains."
    Mattera R., Ritter B., Sidhu S.S., McPherson P.S., Bonifacino J.S.
    J. Biol. Chem. 279:8018-8028(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GGA1; GGA2; GGA3 AND AP1G1.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-181, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiNECP2_MOUSE
AccessioniPrimary (citable) accession number: Q9D1J1
Secondary accession number(s): Q3U1M1, Q9CV41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: June 1, 2001
Last modified: March 16, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.