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Protein

Microfibril-associated glycoprotein 4

Gene

Mfap4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Could be involved in calcium-dependent cell adhesion or intercellular interactions. May contribute to the elastic fiber assembly and/or maintenance.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-MMU-2129379. Molecules associated with elastic fibres.

Names & Taxonomyi

Protein namesi
Recommended name:
Microfibril-associated glycoprotein 4
Gene namesi
Name:Mfap4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1342276. Mfap4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000000913523 – 257Microfibril-associated glycoprotein 4Add BLAST235

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi89N-linked (GlcNAc...)Sequence analysis1
Glycosylationi139N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9D1H9.
PeptideAtlasiQ9D1H9.
PRIDEiQ9D1H9.

PTM databases

PhosphoSitePlusiQ9D1H9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000042436.
CleanExiMM_MFAP4.
GenevisibleiQ9D1H9. MM.

Interactioni

Subunit structurei

Homodimer. Can also form higher oligomers. Interacts with FBN1, FBN2 and LOX. Interacts with COL1A1 in a Ca (2+)-dependent manner. Interacts with ELN in a Ca (2+)-dependent manner; this interaction promotes ELN self-assembly.By similarity

Protein-protein interaction databases

IntActiQ9D1H9. 1 interactor.
MINTiMINT-4126576.
STRINGi10090.ENSMUSP00000070848.

Structurei

3D structure databases

ProteinModelPortaliQ9D1H9.
SMRiQ9D1H9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 257Fibrinogen C-terminalPROSITE-ProRule annotationAdd BLAST224

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi28 – 30Cell attachment siteSequence analysis3

Sequence similaritiesi

Contains 1 fibrinogen C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2579. Eukaryota.
ENOG410ZYS4. LUCA.
GeneTreeiENSGT00830000128240.
HOGENOMiHOG000037127.
HOVERGENiHBG001644.
InParanoidiQ9D1H9.
OMAiGINWAQW.
OrthoDBiEOG091G03M1.
PhylomeDBiQ9D1H9.
TreeFamiTF336658.

Family and domain databases

CDDicd00087. FReD. 1 hit.
Gene3Di3.90.215.10. 1 hit.
4.10.530.10. 1 hit.
InterProiIPR014716. Fibrinogen_a/b/g_C_1.
IPR014715. Fibrinogen_a/b/g_C_2.
IPR002181. Fibrinogen_a/b/g_C_dom.
[Graphical view]
PfamiPF00147. Fibrinogen_C. 1 hit.
[Graphical view]
SMARTiSM00186. FBG. 1 hit.
[Graphical view]
SUPFAMiSSF56496. SSF56496. 1 hit.
PROSITEiPS51406. FIBRINOGEN_C_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D1H9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKALPALPLM LMLLSMPPPC APQASGIRGD ALEKSCLQQP LDCDDIYAQG
60 70 80 90 100
YQEDGVYLIY PYGPSVPVPV FCDMTTEGGK WTVFQKRFNG SVSFFRGWSD
110 120 130 140 150
YKLGFGRADG EYWLGLQNLH LLTLKQKYEL RVDLEDFENN TAYAKYIDFS
160 170 180 190 200
ISPNAISAEE DGYTLYVAGF EDGGAGDSLS YHSGQKFSTF DRDQDLFVQN
210 220 230 240 250
CAALSSGAFW FRSCHFANLN GFYLGGSHLS YANGINWAQW KGFYYSLKRT

EMKIRRA
Length:257
Mass (Da):28,938
Last modified:June 1, 2001 - v1
Checksum:iA1E80B9671399766
GO
Isoform 2 (identifier: Q9D1H9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     115-115: G → GKVGPWGGGCPSAKSLLTGCHPSLG

Note: Gene prediction confirmed by EST data.
Show »
Length:281
Mass (Da):31,229
Checksum:i2347D6F27839181F
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013482115G → GKVGPWGGGCPSAKSLLTGC HPSLG in isoform 2. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003537 mRNA. Translation: BAB22844.1.
AL604029 Genomic DNA. Translation: CAI24180.1.
AL604029 Genomic DNA. Translation: CAI24181.1.
BC022666 mRNA. Translation: AAH22666.1.
CCDSiCCDS24813.1. [Q9D1H9-1]
RefSeqiNP_083844.1. NM_029568.2. [Q9D1H9-1]
XP_006534485.1. XM_006534422.3. [Q9D1H9-2]
UniGeneiMm.272278.

Genome annotation databases

EnsembliENSMUST00000040522; ENSMUSP00000038971; ENSMUSG00000042436. [Q9D1H9-2]
ENSMUST00000064783; ENSMUSP00000070848; ENSMUSG00000042436. [Q9D1H9-1]
GeneIDi76293.
KEGGimmu:76293.
UCSCiuc007jhm.1. mouse. [Q9D1H9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003537 mRNA. Translation: BAB22844.1.
AL604029 Genomic DNA. Translation: CAI24180.1.
AL604029 Genomic DNA. Translation: CAI24181.1.
BC022666 mRNA. Translation: AAH22666.1.
CCDSiCCDS24813.1. [Q9D1H9-1]
RefSeqiNP_083844.1. NM_029568.2. [Q9D1H9-1]
XP_006534485.1. XM_006534422.3. [Q9D1H9-2]
UniGeneiMm.272278.

3D structure databases

ProteinModelPortaliQ9D1H9.
SMRiQ9D1H9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9D1H9. 1 interactor.
MINTiMINT-4126576.
STRINGi10090.ENSMUSP00000070848.

PTM databases

PhosphoSitePlusiQ9D1H9.

Proteomic databases

PaxDbiQ9D1H9.
PeptideAtlasiQ9D1H9.
PRIDEiQ9D1H9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040522; ENSMUSP00000038971; ENSMUSG00000042436. [Q9D1H9-2]
ENSMUST00000064783; ENSMUSP00000070848; ENSMUSG00000042436. [Q9D1H9-1]
GeneIDi76293.
KEGGimmu:76293.
UCSCiuc007jhm.1. mouse. [Q9D1H9-1]

Organism-specific databases

CTDi4239.
MGIiMGI:1342276. Mfap4.

Phylogenomic databases

eggNOGiKOG2579. Eukaryota.
ENOG410ZYS4. LUCA.
GeneTreeiENSGT00830000128240.
HOGENOMiHOG000037127.
HOVERGENiHBG001644.
InParanoidiQ9D1H9.
OMAiGINWAQW.
OrthoDBiEOG091G03M1.
PhylomeDBiQ9D1H9.
TreeFamiTF336658.

Enzyme and pathway databases

ReactomeiR-MMU-2129379. Molecules associated with elastic fibres.

Miscellaneous databases

ChiTaRSiMfap4. mouse.
PROiQ9D1H9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042436.
CleanExiMM_MFAP4.
GenevisibleiQ9D1H9. MM.

Family and domain databases

CDDicd00087. FReD. 1 hit.
Gene3Di3.90.215.10. 1 hit.
4.10.530.10. 1 hit.
InterProiIPR014716. Fibrinogen_a/b/g_C_1.
IPR014715. Fibrinogen_a/b/g_C_2.
IPR002181. Fibrinogen_a/b/g_C_dom.
[Graphical view]
PfamiPF00147. Fibrinogen_C. 1 hit.
[Graphical view]
SMARTiSM00186. FBG. 1 hit.
[Graphical view]
SUPFAMiSSF56496. SSF56496. 1 hit.
PROSITEiPS51406. FIBRINOGEN_C_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMFAP4_MOUSE
AccessioniPrimary (citable) accession number: Q9D1H9
Secondary accession number(s): Q5NCN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.