Q9D1H7 (GET4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Golgi to ER traffic protein 4 homolog

Gene names

Name:

Get4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting By similarity.
Subunit structure	Component of the BAT3 complex, at least composed of BAG6/BAT3, UBL4A and GET3/TRC35 By similarity.
Subcellular location	Cytoplasm › cytosol By similarity.
Sequence similarities	Belongs to the GET4 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tail-anchored membrane protein insertion into ER membrane Inferred from sequence or structural similarity. Source: UniProtKB transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	BAT3 complex Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9D1H7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q9D1H7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-53: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 327	326	Golgi to ER traffic protein 4 homolog		PRO_0000228105
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
Natural variations
	Alternative sequence	1 – 53	53	Missing in isoform 2.		VSP_017653

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 2. Checksum: 04B8DCC8E75FB022	FASTA	327	36,525
	10 20 30 40 50 60 MAAAAAMAEQ EGARNGARNR GGVQRVEGKL RASVEKGDYY EAHQMYRTLF FRYMSQSKHA 70 80 90 100 110 120 EARELMYSGA LLFFSHGQQN SAADLSMLVL ESLEKAEVDV ADELLENLAK VFSLMDPNSP 130 140 150 160 170 180 ERVAFVSRAL KWSSGGSGKL GHPRLHQLLA LTLWKEQNYC ESRYHFLHSS DGEGCANMLV 190 200 210 220 230 240 EYSTARGFRS EVDMFVAQAV LQFLCLKNKN SALVVFTTYT QKHPSIEDGP PFVQPLLNFI 250 260 270 280 290 300 WFLLLAVDGG KLAVFTVLCE QYQPSLRRDP MYNEYLDRIG QLFFGVPPKQ TSSYGGLLGN 310 320 LLSSLMGSSE QEEGEESQDD SSPIELD
	Isoform 2 [UniParc]. Checksum: 185B7840B12D0698	FASTA	274	30,560
	10 20 30 40 50 60 MSQSKHAEAR ELMYSGALLF FSHGQQNSAA DLSMLVLESL EKAEVDVADE LLENLAKVFS 70 80 90 100 110 120 LMDPNSPERV AFVSRALKWS SGGSGKLGHP RLHQLLALTL WKEQNYCESR YHFLHSSDGE 130 140 150 160 170 180 GCANMLVEYS TARGFRSEVD MFVAQAVLQF LCLKNKNSAL VVFTTYTQKH PSIEDGPPFV 190 200 210 220 230 240 QPLLNFIWFL LLAVDGGKLA VFTVLCEQYQ PSLRRDPMYN EYLDRIGQLF FGVPPKQTSS 250 260 270 YGGLLGNLLS SLMGSSEQEE GEESQDDSSP IELD

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Embryo.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: FVB/N. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK003551 mRNA. Translation: BAB22851.2. BC019557 mRNA. Translation: AAH19557.1.
IPI	IPI00272521. IPI00742325.
RefSeq	NP_001156788.1. NM_001163316.1. NP_080545.2. NM_026269.2.
UniGene	Mm.270137.
3D structure databases
ProteinModelPortal	Q9D1H7.
SMR	Q9D1H7. Positions 21-291.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9D1H7.
Proteomic databases
PRIDE	Q9D1H7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000026976; ENSMUSP00000026976; ENSMUSG00000025858. ENSMUST00000110878; ENSMUSP00000106502; ENSMUSG00000025858.
GeneID	67604.
KEGG	mmu:67604.
NMPDR	fig|10090.3.peg.12689.
UCSC	uc009agg.2. mouse. uc009agh.2. mouse.
Organism-specific databases
CTD	51608.
MGI	MGI:1914854. Get4.
Phylogenomic databases
eggNOG	roNOG08212.
GeneTree	ENSGT00390000015750.
HOGENOM	HBG314325.
HOVERGEN	HBG059877.
InParanoid	Q9D1H7.
OMA	KRDPMYN.
OrthoDB	EOG4SXND1.
PhylomeDB	Q9D1H7.
Gene expression databases
ArrayExpress	Q9D1H7.
Bgee	Q9D1H7.
CleanEx	MM_1110007L15RIK.
Genevestigator	Q9D1H7.
GermOnline	ENSMUSG00000025858. Mus musculus.
Family and domain databases
InterPro	IPR007317. UPF0363. [Graphical view]
PANTHER	PTHR12875. DUF410. 1 hit.
Pfam	PF04190. DUF410. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	325021.
SOURCE	Search...

Entry information

Entry name

GET4_MOUSE

Accession

Primary (citable) accession number: Q9D1H7
Secondary accession number(s): Q78J86

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	July 5, 2004
Last modified:	September 21, 2011
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program