Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ras-related protein Rab-1B

Gene

Rab1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1B regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum.By similarity

Miscellaneous

Rab-1B binds GTP and GDP and possesses intrinsic GTPase activity.By similarity

Enzyme regulationi

Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP).Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 23GTPBy similarity9
Nucleotide bindingi33 – 40GTPBy similarity8
Nucleotide bindingi63 – 67GTPBy similarity5
Nucleotide bindingi121 – 124GTPBy similarity4
Nucleotide bindingi151 – 153GTPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAutophagy, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-6807878. COPI-mediated anterograde transport.
R-MMU-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-MMU-8873719. RAB geranylgeranylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-1B
Gene namesi
Name:Rab1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1923558. Rab1b.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001210621 – 201Ras-related protein Rab-1BAdd BLAST201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei76O-(2-cholinephosphoryl)serineBy similarity1
Lipidationi200S-geranylgeranyl cysteineBy similarity1
Modified residuei201Cysteine methyl esterSequence analysis1
Lipidationi201S-geranylgeranyl cysteineBy similarity1

Post-translational modificationi

Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1).By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiQ9D1G1.
MaxQBiQ9D1G1.
PaxDbiQ9D1G1.
PRIDEiQ9D1G1.

PTM databases

iPTMnetiQ9D1G1.
PhosphoSitePlusiQ9D1G1.
SwissPalmiQ9D1G1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024870.
CleanExiMM_RAB1B.
ExpressionAtlasiQ9D1G1. baseline and differential.
GenevisibleiQ9D1G1. MM.

Interactioni

Subunit structurei

Interacts with MICAL1 and MICAL2. Interacts (GTP-bound form) with MICALCL, MICAL1 and MILCAL3. Interacts with GDI1; the interaction requires the GDP-bound state. Interacts with CHM/REP1; the interaction requires the GDP-bound form and is necessary for prenylation by GGTase II.By similarity

Protein-protein interaction databases

BioGridi218071. 1 interactor.
IntActiQ9D1G1. 17 interactors.
MINTiMINT-4998063.
STRINGi10090.ENSMUSP00000025804.

Structurei

3D structure databases

ProteinModelPortaliQ9D1G1.
SMRiQ9D1G1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni64 – 83Switch 2 region; required for interaction with REP1/CHMBy similarityAdd BLAST20

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi37 – 45Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0084. Eukaryota.
ENOG410XQN5. LUCA.
GeneTreeiENSGT00890000139330.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ9D1G1.
KOiK07875.
OMAiGQPIQQN.
OrthoDBiEOG091G0LA6.
PhylomeDBiQ9D1G1.
TreeFamiTF300097.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
PfamiView protein in Pfam
PF00071. Ras. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51419. RAB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9D1G1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPEYDYLFK LLLIGDSGVG KSCLLLRFAD DTYTESYIST IGVDFKIRTI
60 70 80 90 100
ELDGKTIKLQ IWDTAGQERF RTITSSYYRG AHGIIVVYDV TDQESYANVK
110 120 130 140 150
QWLQEIDRYA SENVNKLLVG NKSDLTTKKV VDNTTAKEFA DSLGVPFLET
160 170 180 190 200
SAKNATNVEQ AFMTMAAEIK KRMGPGAASG GERPNLKIDS TPVKPASGGC

C
Length:201
Mass (Da):22,187
Last modified:June 1, 2001 - v1
Checksum:i870DFF52AEF4B2BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003609 mRNA. Translation: BAB22888.1.
AK156726 mRNA. Translation: BAE33821.1.
BC016408 mRNA. Translation: AAH16408.1.
CCDSiCCDS29452.1.
RefSeqiNP_083852.1. NM_029576.3.
UniGeneiMm.182563.
Mm.297430.

Genome annotation databases

EnsembliENSMUST00000025804; ENSMUSP00000025804; ENSMUSG00000024870.
GeneIDi76308.
KEGGimmu:76308.
UCSCiuc008gcf.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiRAB1B_MOUSE
AccessioniPrimary (citable) accession number: Q9D1G1
Secondary accession number(s): Q3U0N1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2001
Last modified: August 30, 2017
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families