SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9D1F4

- AKTS1_MOUSE

UniProt

Q9D1F4 - AKTS1_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Proline-rich AKT1 substrate 1
Gene
Akt1s1, Pras
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.2 Publications

GO - Biological processi

  1. Notch signaling pathway Source: MGI
  2. negative regulation of TOR signaling Source: InterPro
  3. negative regulation of cell size Source: Ensembl
  4. negative regulation of protein kinase activity Source: Ensembl
  5. neurotrophin TRK receptor signaling pathway Source: InterPro
  6. regulation of neuron apoptotic process Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
REACT_213550. HSF1-dependent transactivation.
REACT_220918. AKT phosphorylates targets in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline-rich AKT1 substrate 1
Short name:
Proline-rich AKT substrate
Gene namesi
Name:Akt1s1
Synonyms:Pras
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1914855. Akt1s1.

Subcellular locationi

Cytoplasmcytosol
Note: Found in the cytosolic fraction of the brain. Colocalizes with cortical neurons following ischemic/reperfusion injury.1 Publication

GO - Cellular componenti

  1. TORC1 complex Source: Ensembl
  2. cytosol Source: UniProtKB
  3. nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 257257Proline-rich AKT1 substrate 1
PRO_0000253447Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei88 – 881Phosphoserine By similarity
Modified residuei92 – 921Phosphoserine By similarity
Modified residuei184 – 1841Phosphoserine By similarity
Modified residuei203 – 2031Phosphoserine1 Publication
Modified residuei204 – 2041Phosphoserine1 Publication
Modified residuei212 – 2121Phosphoserine1 Publication
Modified residuei213 – 2131Phosphoserine1 Publication
Modified residuei247 – 2471Phosphothreonine; by PKB/AKT1 By similarityBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9D1F4.
PaxDbiQ9D1F4.
PRIDEiQ9D1F4.

PTM databases

PhosphoSiteiQ9D1F4.

Expressioni

Gene expression databases

ArrayExpressiQ9D1F4.
BgeeiQ9D1F4.
CleanExiMM_AKT1S1.
GenevestigatoriQ9D1F4.

Interactioni

Subunit structurei

Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Interacts directly with RPTOR. The phosphorylated form interacts with 14-3-3 proteins and with phosphorylated AKT1 following transient focal cerebral ischemia in vivo.1 Publication

Protein-protein interaction databases

IntActiQ9D1F4. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9D1F4.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi35 – 439Poly-Pro
Compositional biasi77 – 9721Pro-rich
Add
BLAST

Phylogenomic databases

eggNOGiNOG40718.
GeneTreeiENSGT00390000017397.
HOGENOMiHOG000236322.
HOVERGENiHBG059465.
InParanoidiA2RT52.
KOiK16184.
PhylomeDBiQ9D1F4.

Family and domain databases

InterProiIPR026682. AKT1S1.
[Graphical view]
PANTHERiPTHR21844. PTHR21844. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9D1F4-1 [UniParc]FASTAAdd to Basket

« Hide

MASGRPEELW EAVVGAAERF QARTGTELVL LTAAPPPPPR PGPCAYAAHG    50
RGALAEAARR CLHDIAQAHR AATATRPPGP PPAPQPPSPA PSPPPRPALA 100
REDEEEDEDE PTETETSGER LGGSDNGGLF MMDEDATLQD LPPFCESDPE 150
STDDGSLSEE TPAGPTACPQ PPATALPTQQ YAKSLPVSVP VWAFKEKRTE 200
ARSSDEENGP PSSPDLDRIA ASMRALVLRE AEDTQVFGDL PRPRLNTSDF 250
QKLKRKY 257
Length:257
Mass (Da):27,483
Last modified:June 1, 2001 - v1
Checksum:i4BFFC8630DBBA5EC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK003638 mRNA. Translation: BAB22905.1.
AK141771 mRNA. Translation: BAE24829.1.
AK154110 mRNA. Translation: BAE32383.1.
BC132372 mRNA. Translation: AAI32373.1.
BC132374 mRNA. Translation: AAI32375.1.
CCDSiCCDS21218.1.
RefSeqiNP_001240849.1. NM_001253920.1.
NP_001277623.1. NM_001290694.1.
NP_001277624.1. NM_001290695.1.
NP_080546.1. NM_026270.4.
XP_006541182.1. XM_006541119.1.
XP_006541183.1. XM_006541120.1.
XP_006541184.1. XM_006541121.1.
XP_006541185.1. XM_006541122.1.
UniGeneiMm.148007.
Mm.491121.

Genome annotation databases

EnsembliENSMUST00000054343; ENSMUSP00000049764; ENSMUSG00000011096.
ENSMUST00000107880; ENSMUSP00000103512; ENSMUSG00000011096.
GeneIDi67605.
KEGGimmu:67605.
UCSCiuc009grd.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK003638 mRNA. Translation: BAB22905.1 .
AK141771 mRNA. Translation: BAE24829.1 .
AK154110 mRNA. Translation: BAE32383.1 .
BC132372 mRNA. Translation: AAI32373.1 .
BC132374 mRNA. Translation: AAI32375.1 .
CCDSi CCDS21218.1.
RefSeqi NP_001240849.1. NM_001253920.1.
NP_001277623.1. NM_001290694.1.
NP_001277624.1. NM_001290695.1.
NP_080546.1. NM_026270.4.
XP_006541182.1. XM_006541119.1.
XP_006541183.1. XM_006541120.1.
XP_006541184.1. XM_006541121.1.
XP_006541185.1. XM_006541122.1.
UniGenei Mm.148007.
Mm.491121.

3D structure databases

ProteinModelPortali Q9D1F4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9D1F4. 1 interaction.

PTM databases

PhosphoSitei Q9D1F4.

Proteomic databases

MaxQBi Q9D1F4.
PaxDbi Q9D1F4.
PRIDEi Q9D1F4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000054343 ; ENSMUSP00000049764 ; ENSMUSG00000011096 .
ENSMUST00000107880 ; ENSMUSP00000103512 ; ENSMUSG00000011096 .
GeneIDi 67605.
KEGGi mmu:67605.
UCSCi uc009grd.2. mouse.

Organism-specific databases

CTDi 84335.
MGIi MGI:1914855. Akt1s1.

Phylogenomic databases

eggNOGi NOG40718.
GeneTreei ENSGT00390000017397.
HOGENOMi HOG000236322.
HOVERGENi HBG059465.
InParanoidi A2RT52.
KOi K16184.
PhylomeDBi Q9D1F4.

Enzyme and pathway databases

Reactomei REACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
REACT_213550. HSF1-dependent transactivation.
REACT_220918. AKT phosphorylates targets in the cytosol.

Miscellaneous databases

ChiTaRSi AKT1S1. mouse.
NextBioi 325025.
PROi Q9D1F4.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9D1F4.
Bgeei Q9D1F4.
CleanExi MM_AKT1S1.
Genevestigatori Q9D1F4.

Family and domain databases

InterProi IPR026682. AKT1S1.
[Graphical view ]
PANTHERi PTHR21844. PTHR21844. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Embryo.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  3. "Neuroprotective role of a proline-rich Akt substrate in apoptotic neuronal cell death after stroke: relationships with nerve growth factor."
    Saito A., Narasimhan P., Hayashi T., Okuno S., Ferrand-Drake M., Chan P.H.
    J. Neurosci. 24:1584-1593(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  5. "Modulation of proline-rich akt substrate survival signaling pathways by oxidative stress in mouse brains after transient focal cerebral ischemia."
    Saito A., Hayashi T., Okuno S., Nishi T., Chan P.H.
    Stroke 37:513-517(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203; SER-204; SER-212 AND SER-213, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiAKTS1_MOUSE
AccessioniPrimary (citable) accession number: Q9D1F4
Secondary accession number(s): A2RT52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi