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Q9D1E8

- PLCE_MOUSE

UniProt

Q9D1E8 - PLCE_MOUSE

Protein

1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon

Gene

Agpat5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (16 May 2003)
      Previous versions | rss
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    Functioni

    Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Acts on LPA containing saturated or unsaturated fatty acids C15:0-C20:4 at the sn-1 position using C18:1-CoA as the acyl donor. Also acts on lysophosphatidylethanolamine using oleoyl-CoA, but not arachidonoyl-CoA, and lysophosphatidylinositol using arachidonoyl-CoA, but not oleoyl-CoA. Activity toward lysophosphatidylglycerol not detectable.1 Publication

    Catalytic activityi

    Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.

    Pathwayi

    GO - Molecular functioni

    1. 1-acylglycerol-3-phosphate O-acyltransferase activity Source: MGI

    GO - Biological processi

    1. acylglycerol metabolic process Source: MGI
    2. CDP-diacylglycerol biosynthetic process Source: UniProtKB-UniPathway
    3. hematopoietic progenitor cell differentiation Source: MGI

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Enzyme and pathway databases

    ReactomeiREACT_188640. Synthesis of PA.
    UniPathwayiUPA00557; UER00613.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon (EC:2.3.1.51)
    Alternative name(s):
    1-acylglycerol-3-phosphate O-acyltransferase 5
    Short name:
    1-AGP acyltransferase 5
    Short name:
    1-AGPAT 5
    Lysophosphatidic acid acyltransferase epsilon
    Short name:
    LPAAT-epsilon
    Gene namesi
    Name:Agpat5
    Synonyms:D8Ertd319e
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1196345. Agpat5.

    Subcellular locationi

    Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity. Nucleus envelope By similarity. Mitochondrion By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. mitochondrion Source: MGI
    4. nuclear envelope Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Mitochondrion, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3653651-acyl-sn-glycerol-3-phosphate acyltransferase epsilonPRO_0000208201Add
    BLAST

    Proteomic databases

    MaxQBiQ9D1E8.
    PaxDbiQ9D1E8.
    PRIDEiQ9D1E8.

    PTM databases

    PhosphoSiteiQ9D1E8.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Gene expression databases

    ArrayExpressiQ9D1E8.
    BgeeiQ9D1E8.
    GenevestigatoriQ9D1E8.

    Interactioni

    Protein-protein interaction databases

    BioGridi206392. 1 interaction.
    IntActiQ9D1E8. 1 interaction.
    STRINGi10090.ENSMUSP00000117025.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9D1E8.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei15 – 3521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei345 – 36521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi93 – 986HXXXXD motif

    Domaini

    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0204.
    GeneTreeiENSGT00530000062943.
    HOGENOMiHOG000007801.
    HOVERGENiHBG039632.
    InParanoidiQ9D1E8.
    OMAiDWIIADM.
    OrthoDBiEOG7QK0C7.
    PhylomeDBiQ9D1E8.
    TreeFamiTF314346.

    Family and domain databases

    InterProiIPR002123. Plipid/glycerol_acylTrfase.
    [Graphical view]
    PfamiPF01553. Acyltransferase. 1 hit.
    [Graphical view]
    SMARTiSM00563. PlsC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9D1E8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLSLVLHTY SMRYLLPSVL LLGSAPTYLL AWTLWRVLSA LMPARLYQRV    50
    DDRLYCVYQN MVLFFFENYT GVQILLYGDL PKNKENVIYL ANHQSTVDWI 100
    VADMLAARQD ALGHVRYVLK DKLKWLPLYG FYFAQHGGIY VKRSAKFNDK 150
    EMRSKLQSYV NAGTPMYLVI FPEGTRYNAT YTKLLSASQA FAAQRGLAVL 200
    KHVLTPRIKA THVAFDSMKS HLDAIYDVTV VYEGNEKGSG KYSNPPSMTE 250
    FLCKQCPKLH IHFDRIDRNE VPEEQEHMKK WLHERFEIKD RLLIEFYDSP 300
    DPERRNKFPG KSVHSRLSVK KTLPSVLILG SLTAVMLMTE SGRKLYMGTW 350
    LYGTLLGCLW FVIKA 365
    Length:365
    Mass (Da):42,202
    Last modified:May 16, 2003 - v2
    Checksum:iC13E14759610E19B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti80 – 801L → W in AAN75571. 1 PublicationCurated
    Sequence conflicti128 – 1281L → M in AAN75571. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY161042 mRNA. Translation: AAN75571.1.
    AK003649 mRNA. Translation: BAB22915.2.
    AK082137 mRNA. Translation: BAC38421.1.
    AK089885 mRNA. Translation: BAC40983.1.
    AK152709 mRNA. Translation: BAE31436.1.
    AK152889 mRNA. Translation: BAE31572.1.
    AK155378 mRNA. Translation: BAE33229.1.
    BC031987 mRNA. Translation: AAH31987.2.
    CCDSiCCDS22126.1.
    RefSeqiNP_081068.1. NM_026792.3.
    UniGeneiMm.24117.

    Genome annotation databases

    EnsembliENSMUST00000149565; ENSMUSP00000117025; ENSMUSG00000031467.
    GeneIDi52123.
    KEGGimmu:52123.
    UCSCiuc009kzv.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY161042 mRNA. Translation: AAN75571.1 .
    AK003649 mRNA. Translation: BAB22915.2 .
    AK082137 mRNA. Translation: BAC38421.1 .
    AK089885 mRNA. Translation: BAC40983.1 .
    AK152709 mRNA. Translation: BAE31436.1 .
    AK152889 mRNA. Translation: BAE31572.1 .
    AK155378 mRNA. Translation: BAE33229.1 .
    BC031987 mRNA. Translation: AAH31987.2 .
    CCDSi CCDS22126.1.
    RefSeqi NP_081068.1. NM_026792.3.
    UniGenei Mm.24117.

    3D structure databases

    ProteinModelPortali Q9D1E8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 206392. 1 interaction.
    IntActi Q9D1E8. 1 interaction.
    STRINGi 10090.ENSMUSP00000117025.

    PTM databases

    PhosphoSitei Q9D1E8.

    Proteomic databases

    MaxQBi Q9D1E8.
    PaxDbi Q9D1E8.
    PRIDEi Q9D1E8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000149565 ; ENSMUSP00000117025 ; ENSMUSG00000031467 .
    GeneIDi 52123.
    KEGGi mmu:52123.
    UCSCi uc009kzv.1. mouse.

    Organism-specific databases

    CTDi 55326.
    MGIi MGI:1196345. Agpat5.

    Phylogenomic databases

    eggNOGi COG0204.
    GeneTreei ENSGT00530000062943.
    HOGENOMi HOG000007801.
    HOVERGENi HBG039632.
    InParanoidi Q9D1E8.
    OMAi DWIIADM.
    OrthoDBi EOG7QK0C7.
    PhylomeDBi Q9D1E8.
    TreeFami TF314346.

    Enzyme and pathway databases

    UniPathwayi UPA00557 ; UER00613 .
    Reactomei REACT_188640. Synthesis of PA.

    Miscellaneous databases

    NextBioi 308524.
    PROi Q9D1E8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9D1E8.
    Bgeei Q9D1E8.
    Genevestigatori Q9D1E8.

    Family and domain databases

    InterProi IPR002123. Plipid/glycerol_acylTrfase.
    [Graphical view ]
    Pfami PF01553. Acyltransferase. 1 hit.
    [Graphical view ]
    SMARTi SM00563. PlsC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of 1-acylglycerolphosphate acyltransferase-epsilon."
      Lu B., Jiang Y.J., Chan M., Choy P.C.
      Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: NMRI.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Bone marrow, Cerebellum, Dendritic cell, Embryo and Spleen.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary gland.
    4. "Cloning and characterization of murine 1-acyl-sn-glycerol 3-phosphate acyltransferases and their regulation by PPARalpha in murine heart."
      Lu B., Jiang Y.J., Zhou Y., Xu F.Y., Hatch G.M., Choy P.C.
      Biochem. J. 385:469-477(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
      Strain: C57BL/6J.

    Entry informationi

    Entry nameiPLCE_MOUSE
    AccessioniPrimary (citable) accession number: Q9D1E8
    Secondary accession number(s): Q3U702, Q8BG61, Q8CGN6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2002
    Last sequence update: May 16, 2003
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    It is uncertain whether Met-1 or Met-12 is the initiator.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3