Q9D1C2 (CBY1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein chibby homolog 1

Alternative name(s):
Cytosolic leucine-rich protein
PIGEA-14
PKD2 interactor, Golgi and endoplasmic reticulum-associated 1

Gene names

Name:	Cby1
Synonyms:	Cby, Pgea1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	127 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Inhibits the Wnt/Wingless pathway by binding to beta-catenin and inhibiting beta-catenin-mediated transcriptional activation through competition with TCF/LEF transcription factors. Has also been shown to play a role in regulating the intracellular trafficking of polycystin-2/PKD2 and possibly of other intracellular proteins By similarity. Promotes adipocyte and cardiomyocyte differentiation. Ref.4 Ref.5
Subunit structure	Homodimer. Interacts with polycystin-2/PKD2 and GM130. Interacts with the C-terminal region of beta-catenin By similarity.
Subcellular location	Nucleus speckle By similarity. Golgi apparatus › trans-Golgi network By similarity. Note: Nuclear, in a punctate manner. Also found in the trans-Golgi By similarity.
Tissue specificity	Found in heart, brain, lung, liver, muscle, kidney and testis. Levels are approximately 3-fold higher in embryonic and adult heart than in lung or liver. Ref.4
Developmental stage	Ubiquitously expressed in early stages of embryonic stem cell differentiation but decreases at later stages when high expression is restricted to cardiomyocytes. Ref.4
Miscellaneous	'Chibby' is Japanese for 'small'; the gene was so named for the RNAi phenotype seen in flies.
Sequence similarities	Belongs to the chibby family.

Ontologies

Keywords
Biological process	Differentiation
Cellular component	Golgi apparatus Nucleus
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cardiac muscle cell differentiation Inferred from mutant phenotype Ref.4. Source: UniProtKB cilium assembly Inferred from mutant phenotype PubMed 19364920. Source: MGI fat cell differentiation Inferred from mutant phenotype Ref.5. Source: UniProtKB negative regulation of canonical Wnt receptor signaling pathway Inferred from mutant phenotype PubMed 19364920. Source: MGI negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara protein localization Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule basal body Inferred from direct assay PubMed 19364920. Source: MGI nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell trans-Golgi network Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 127	127	Protein chibby homolog 1		PRO_0000058355
Regions
	Region	60 – 112	53	Minimal region for the interaction with PKD2 By similarity
	Coiled coil	68 – 110	43	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9D1C2 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 7CE38A48D045CCC7	FASTA	127	14,535
	10 20 30 40 50 60 MPLFGSIFSP KKTPPRKSAS LSNLHSLDRS TRELELGLDY GTPTMNLAGQ SLKFENGQWV 70 80 90 100 110 120 ADSVISGGVD RRETQRLRKR NQQLEEENNL LRLKVDILLD MLSETTAESH LKDKELDELK VTNRRRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel cytosolic leucine-rich protein." Huang C.-H. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[4]	"Chibby, an antagonist of the Wnt/beta-catenin pathway, facilitates cardiomyocyte differentiation of murine embryonic stem cells." Singh A.M., Li F.-Q., Hamazaki T., Kasahara H., Takemaru K., Terada N. Circulation 115:617-626(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[5]	"Chibby promotes adipocyte differentiation through inhibition of beta-catenin signaling." Li F.-Q., Singh A.M., Mofunanya A., Love D., Terada N., Moon R.T., Takemaru K. Mol. Cell. Biol. 27:4347-4354(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF331040 mRNA. Translation: AAL56061.1. AK003719 mRNA. Translation: BAB22956.1. BC005733 mRNA. Translation: AAH05733.1.
IPI	IPI00133582.
RefSeq	NP_082910.1. NM_028634.3.
UniGene	Mm.294441.
3D structure databases
ProteinModelPortal	Q9D1C2.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000023064.
PTM databases
PhosphoSite	Q9D1C2.
Proteomic databases
PaxDb	Q9D1C2.
PRIDE	Q9D1C2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000023064; ENSMUSP00000023064; ENSMUSG00000022428.
GeneID	73739.
KEGG	mmu:73739.
UCSC	uc007wuc.1. mouse.
Organism-specific databases
CTD	25776.
MGI	MGI:1920989. Cby1.
Phylogenomic databases
eggNOG	NOG39404.
GeneTree	ENSGT00510000047043.
HOGENOM	HOG000006694.
HOVERGEN	HBG050816.
InParanoid	Q9D1C2.
OMA	LRKRNQQ.
OrthoDB	EOG4JT072.
Gene expression databases
Bgee	Q9D1C2.
CleanEx	MM_CBY1.
Genevestigator	Q9D1C2.
GermOnline	ENSMUSG00000022428. Mus musculus.
Family and domain databases
ProtoNet	Search...
Other
NextBio	338953.
SOURCE	Search...

Entry information

Entry name

CBY1_MOUSE

Accession

Primary (citable) accession number: Q9D1C2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program