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Protein

Cytosolic non-specific dipeptidase

Gene

Cndp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly. Catalyzes the production of N-lactoyl-amino acids from lactate and amino acids by reverse proteolysis.By similarity

Catalytic activityi

Hydrolysis of dipeptides, preferentially hydrophobic dipeptides including prolyl amino acids.1 Publication

Cofactori

Mn2+2 PublicationsNote: Binds 2 manganese ions per subunit.2 Publications

Enzyme regulationi

Inhibited by bestatin.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99Manganese 21 Publication1
Active sitei101By similarity1
Metal bindingi132Manganese 11 Publication1
Metal bindingi132Manganese 21 Publication1
Active sitei166Proton acceptorBy similarity1
Metal bindingi167Manganese 11 Publication1
Metal bindingi195Manganese 21 Publication1
Binding sitei195Substrate; via carbonyl oxygen1
Binding sitei228Substrate; shared with homodimeric partner1
Sitei228Important for catalytic activity1
Binding sitei330Substrate; shared with homodimeric partner1
Binding sitei343Substrate1
Binding sitei417Substrate; via amide nitrogen and carbonyl oxygen1
Metal bindingi445Manganese 11 Publication1
Binding sitei445Substrate1

GO - Molecular functioni

GO - Biological processi

  • proteolysis Source: MGI

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Metalloprotease, Protease
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-174403 Glutathione synthesis and recycling

Protein family/group databases

MEROPSiM20.005

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic non-specific dipeptidase (EC:3.4.13.18)
Alternative name(s):
CNDP dipeptidase 2
Glutamate carboxypeptidase-like protein 1
Gene namesi
Name:Cndp2
Synonyms:Cn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1913304 Cndp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi228H → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001852731 – 475Cytosolic non-specific dipeptidaseAdd BLAST475

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei58PhosphoserineBy similarity1
Modified residuei299PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9D1A2
MaxQBiQ9D1A2
PaxDbiQ9D1A2
PeptideAtlasiQ9D1A2
PRIDEiQ9D1A2

2D gel databases

REPRODUCTION-2DPAGEiQ9D1A2

PTM databases

iPTMnetiQ9D1A2
PhosphoSitePlusiQ9D1A2
SwissPalmiQ9D1A2

Expressioni

Tissue specificityi

Highly expressed in the parafascicular nucleus of the thalamus, tuberomammillary nucleus of the hypothalamus and the mitral cell layer of the olfactory bulb.1 Publication

Gene expression databases

BgeeiENSMUSG00000024644
CleanExiMM_CNDP2
GenevisibleiQ9D1A2 MM

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi211181, 2 interactors
IntActiQ9D1A2, 3 interactors
MINTiQ9D1A2
STRINGi10090.ENSMUSP00000025546

Structurei

Secondary structure

1475
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 13Combined sources13
Helixi15 – 27Combined sources13
Helixi35 – 37Combined sources3
Helixi38 – 54Combined sources17
Beta strandi58 – 62Combined sources5
Beta strandi66 – 68Combined sources3
Beta strandi74 – 76Combined sources3
Beta strandi80 – 85Combined sources6
Beta strandi93 – 99Combined sources7
Helixi107 – 109Combined sources3
Beta strandi119 – 121Combined sources3
Beta strandi124 – 127Combined sources4
Turni128 – 133Combined sources6
Helixi134 – 149Combined sources16
Beta strandi155 – 164Combined sources10
Helixi166 – 168Combined sources3
Helixi173 – 179Combined sources7
Turni180 – 187Combined sources8
Beta strandi190 – 193Combined sources4
Beta strandi199 – 203Combined sources5
Beta strandi205 – 210Combined sources6
Beta strandi212 – 221Combined sources10
Helixi229 – 232Combined sources4
Turni233 – 235Combined sources3
Helixi239 – 247Combined sources9
Turni260 – 265Combined sources6
Helixi271 – 276Combined sources6
Helixi284 – 290Combined sources7
Helixi301 – 309Combined sources9
Beta strandi313 – 322Combined sources10
Beta strandi325 – 327Combined sources3
Beta strandi334 – 344Combined sources11
Helixi350 – 366Combined sources17
Beta strandi372 – 382Combined sources11
Helixi392 – 405Combined sources14
Beta strandi410 – 416Combined sources7
Helixi420 – 428Combined sources9
Beta strandi430 – 434Combined sources5
Beta strandi450 – 452Combined sources3
Helixi453 – 472Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZOFX-ray2.30A/B1-475[»]
2ZOGX-ray1.70A/B1-475[»]
ProteinModelPortaliQ9D1A2
SMRiQ9D1A2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9D1A2

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 167Substrate binding2

Sequence similaritiesi

Belongs to the peptidase M20A family.Curated

Phylogenomic databases

eggNOGiKOG2276 Eukaryota
COG0624 LUCA
GeneTreeiENSGT00390000009682
HOGENOMiHOG000216709
HOVERGENiHBG051103
InParanoidiQ9D1A2
KOiK08660
OMAiHITIPGF
OrthoDBiEOG091G05RU
PhylomeDBiQ9D1A2
TreeFamiTF300633

Family and domain databases

CDDicd05676 M20_dipept_like_CNDP, 1 hit
InterProiView protein in InterPro
IPR001261 ArgE/DapE_CS
IPR017153 CNDP/DUG1
IPR002933 Peptidase_M20
IPR011650 Peptidase_M20_dimer
PfamiView protein in Pfam
PF07687 M20_dimer, 1 hit
PF01546 Peptidase_M20, 1 hit
PIRSFiPIRSF037242 CNDP_dipeptidase, 1 hit
PROSITEiView protein in PROSITE
PS00759 ARGE_DAPE_CPG2_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9D1A2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALKAVFQY IDENQDRYVK KLAEWVAIQS VSAWPEKRGE IRRMMEVAAA
60 70 80 90 100
DVQRLGGSVE LVDIGKQKLP DGSEIPLPPI LLGKLGSDPQ KKTVCIYGHL
110 120 130 140 150
DVQPAALEDG WDSEPFTLVE REGKLYGRGS TDDKGPVAGW MNALEAYQKT
160 170 180 190 200
GQEIPVNLRF CLEGMEESGS EGLDELIFAQ KDKFFKDVDY VCISDNYWLG
210 220 230 240 250
KNKPCITYGL RGICYFFIEV ECSDKDLHSG VYGGSVHEAM TDLISLMGCL
260 270 280 290 300
VDKKGKILIP GINDAVAPVT DEEHALYDHI DFDMEEFAKD VGAETLLHSC
310 320 330 340 350
KKDILMHRWR YPSLSLHGIE GAFSGSGAKT VIPRKVVGKF SIRLVPDMIP
360 370 380 390 400
EVVSEQVSSY LSKKFAELQS PNKFKVYMGH GGKPWVSDFN HPHYQAGRRA
410 420 430 440 450
LKTVFGVEPD LTREGGSIPV TLTFQEATGK NVMLLPVGSA DDGAHSQNEK
460 470
LNRLNYIEGT KMLAAYLYEV SQLKN
Length:475
Mass (Da):52,767
Last modified:June 1, 2001 - v1
Checksum:i086950275A698500
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45M → I in BAE31451 (PubMed:16141072).Curated1
Sequence conflicti105A → T in BAE42789 (PubMed:16141072).Curated1
Sequence conflicti357V → A in BAB21596 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046738 mRNA Translation: BAB21596.1
AK003779 mRNA Translation: BAB22991.1
AK151013 mRNA Translation: BAE30033.1
AK152729 mRNA Translation: BAE31451.1
AK168029 mRNA Translation: BAE40014.1
AK168652 mRNA Translation: BAE40509.1
AK172034 mRNA Translation: BAE42789.1
BC005532 mRNA Translation: AAH05532.1
CCDSiCCDS29383.1
RefSeqiNP_001276460.1, NM_001289531.1
NP_075638.2, NM_023149.3
UniGeneiMm.29646

Genome annotation databases

EnsembliENSMUST00000025546; ENSMUSP00000025546; ENSMUSG00000024644
ENSMUST00000168419; ENSMUSP00000128696; ENSMUSG00000024644
GeneIDi66054
KEGGimmu:66054
UCSCiuc008fut.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCNDP2_MOUSE
AccessioniPrimary (citable) accession number: Q9D1A2
Secondary accession number(s): Q3TA80
, Q3TI32, Q3U7B9, Q99PV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: June 1, 2001
Last modified: February 28, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health