Q9D168 (INT12_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Integrator complex subunit 12
Short name=Int12

Alternative name(s):
PHD finger protein 22

Gene names

Name:	Ints12
Synonyms:	Phf22

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes By similarity.
Subunit structure	Belongs to the multiprotein complex Integrator, at least composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8, INTS9/RC74, INTS10, CPSF3L/INTS11 and INTS12 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Contains 1 PHD-type zinc finger.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	snRNA processing Inferred from sequence or structural similarity. Source: HGNC
Cellular component	integrator complex Inferred from sequence or structural similarity. Source: HGNC
Molecular function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 461

461

Integrator complex subunit 12

PRO_0000059314

Regions

Zinc finger

158 – 214

57

PHD-type

Compositional bias

269 – 439

171

Ser-rich

Amino acid modifications

Modified residue

126

1

Phosphoserine By similarity

Modified residue

127

1

Phosphoserine Ref.3

Modified residue

426

1

Phosphoserine By similarity

Secondary structure

1

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461

Helix Strand Turn

Details...

Helix

154 – 157

4

Beta strand

162 – 164

3

Beta strand

175 – 177

3

Beta strand

179 – 181

3

Beta strand

184 – 186

3

Turn

187 – 189

3

Beta strand

190 – 192

3

Helix

196 – 200

5

Helix

209 – 215

7

Sequences

	Sequence		Length	Mass (Da)
	Q9D168 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 9230385FBD4A9425	FASTA	461	48,568
	10 20 30 40 50 60 MAATVNLELD PIFLKALGFL HSKSKDSAEK LKALLDESLA RGIDSSYRPT QKDVEPPKIS 70 80 90 100 110 120 STKSLSIKQE PKTSSSLPSG SSNGKVLTAE KIKKEAEKRP ADKMKDVTEG IDVPKKPRLE 130 140 150 160 170 180 KPETRSSPIT VQTSKDLSMA DLSSFEETSA DDFAMEMGLA CVVCRQMTVA SGNQLVECQE 190 200 210 220 230 240 CHNLYHQDCH KPQVTDKEVN DPRLVWYCAR CTRQMKRMAQ KTQKPPQKPA PTVVSVTPTV 250 260 270 280 290 300 KDPLVKKPET KLKQETTFLA FKRTEVKPST VISGNSSSNN VSSSVTSGLT GWAAFAAKTS 310 320 330 340 350 360 SAGPSTAKLN SAAQNSSGKP AASSSNQKPV GLTGLATSSK GGIGSKIGSG NSTSPSVPLK 370 380 390 400 410 420 PLPPLTLGKT GLSRSVSCDN VSKVGLPSPS SLVPGGSSQL SGNGNSATTG PSGSTTSKAT 430 440 450 460 SETSSSTSAS LKGPTSQESQ LNAMKRLQMV KKKAAQKKLK K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Embryo.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Eye, Mammary gland and Mammary tumor.
[3]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-127, MASS SPECTROMETRY. Tissue: Liver.
[4]	"Solution structure of PHD domain in protein NP_082203." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 150-224.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK003874 mRNA. Translation: BAB23052.1.
AK155162 mRNA. Translation: BAE33085.1.
BC010657 mRNA. Translation: AAH10657.1.
BC023801 mRNA. Translation: AAH23801.1.
BC046996 mRNA. Translation: AAH46996.1.

IPI

IPI00133241.

RefSeq

NP_082203.1. NM_027927.3.

UniGene

Mm.246726.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WEV	NMR	-	A	150-224	[»]

ProteinModelPortal

Q9D168.

SMR

Q9D168. Positions 148-224.

ModBase

Search...

PTM databases

PhosphoSite

Q9D168.

Proteomic databases

PRIDE

Q9D168.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000029650; ENSMUSP00000029650; ENSMUSG00000028016.

GeneID

71793.

KEGG

mmu:71793.

UCSC

uc008rkj.1. mouse.

Organism-specific databases

CTD

57117.

MGI

MGI:1919043. Ints12.

Phylogenomic databases

eggNOG

roNOG15130.

GeneTree

ENSGT00390000005218.

HOGENOM

HBG714412.

HOVERGEN

HBG081798.

InParanoid

Q9D168.

OMA

CDNVSKV.

OrthoDB

EOG4Q58Q7.

PhylomeDB

Q9D168.

Gene expression databases

ArrayExpress

Q9D168.

Bgee

Q9D168.

CleanEx

MM_INTS12.

Genevestigator

Q9D168.

GermOnline

ENSMUSG00000028016. Mus musculus.

Family and domain databases

InterPro

IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]

Gene3D

G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.

KO

K13149.

Pfam

PF00628. PHD. 1 hit.
[Graphical view]

SMART

SM00249. PHD. 1 hit.
[Graphical view]

SUPFAM

SSF57903. FYVE_PHD_ZnF. 1 hit.

PROSITE

PS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

334534.

SOURCE

Search...

Entry information

Entry name

INT12_MOUSE

Accession

Primary (citable) accession number: Q9D168
Secondary accession number(s): Q3U2Q5, Q921U2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	June 1, 2001
Last modified:	November 16, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program