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Q9D110

- MTHFS_MOUSE

UniProt

Q9D110 - MTHFS_MOUSE

Protein

5-formyltetrahydrofolate cyclo-ligase

Gene

Mthfs

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-CHO-H4PteGlu) to yield 5,10-methenyltetrahydrofolate By similarity.By similarity

    Catalytic activityi

    ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate.

    Cofactori

    Magnesium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei14 – 141ATPBy similarity
    Binding sitei56 – 561Substrate; via carbonyl oxygenBy similarity
    Binding sitei61 – 611SubstrateBy similarity
    Metal bindingi154 – 1541MagnesiumBy similarity
    Metal bindingi189 – 1891MagnesiumBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 145ATPBy similarity
    Nucleotide bindingi145 – 1539ATPBy similarity

    GO - Molecular functioni

    1. 5-formyltetrahydrofolate cyclo-ligase activity Source: UniProtKB
    2. ATP binding Source: UniProtKB-KW
    3. folic acid binding Source: UniProtKB-KW

    GO - Biological processi

    1. folic acid-containing compound biosynthetic process Source: InterPro
    2. tetrahydrofolate metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Folate-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-formyltetrahydrofolate cyclo-ligase (EC:6.3.3.2)
    Alternative name(s):
    5,10-methenyl-tetrahydrofolate synthetase
    Short name:
    MTHFS
    Short name:
    Methenyl-THF synthetase
    Gene namesi
    Name:Mthfs
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1340032. Mthfs.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 2032025-formyltetrahydrofolate cyclo-ligasePRO_0000200276Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ9D110.
    PaxDbiQ9D110.
    PRIDEiQ9D110.

    PTM databases

    PhosphoSiteiQ9D110.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9D110.
    BgeeiQ9D110.
    GenevestigatoriQ9D110.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    BioGridi223657. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9D110.
    SMRiQ9D110. Positions 1-198.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni148 – 1525Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0212.
    GeneTreeiENSGT00390000017791.
    HOGENOMiHOG000007299.
    HOVERGENiHBG052525.
    InParanoidiB2KF82.
    KOiK01934.
    OMAiVLEAPWF.
    PhylomeDBiQ9D110.
    TreeFamiTF313668.

    Family and domain databases

    Gene3Di3.40.50.10420. 1 hit.
    InterProiIPR002698. FTHF_cligase.
    IPR024185. FTHF_cligase-like.
    [Graphical view]
    PANTHERiPTHR23407:SF1. PTHR23407:SF1. 1 hit.
    PfamiPF01812. 5-FTHF_cyc-lig. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006806. FTHF_cligase. 1 hit.
    TIGRFAMsiTIGR02727. MTHFS_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9D110-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAVTVNSAK RGLRAELKQR LRALSAEERL RQSLLLTQKV IAHNQYQNSK    50
    RISIFLSMQD EVETEVIIKD IFKQGKICFI PRYQFQSNHM DMVRLTSSEE 100
    IALLPKTSWN IHQPGEGDVR EEALSTGGLD LIFLPGLGFD KDGNRLGRGK 150
    GYYDTYLKRC VQHQEVKPYT MALAFKEQIC PQIPVDEHDM KVDEVLYEDS 200
    PAS 203
    Length:203
    Mass (Da):23,202
    Last modified:January 23, 2007 - v2
    Checksum:i4C20DC7416CA5304
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK004092 mRNA. Translation: BAB23165.1.
    CT030686, AC135634 Genomic DNA. Translation: CAQ52322.1.
    CCDSiCCDS23394.1.
    RefSeqiNP_081105.1. NM_026829.2.
    UniGeneiMm.486274.

    Genome annotation databases

    EnsembliENSMUST00000085256; ENSMUSP00000082354; ENSMUSG00000066442.
    GeneIDi107885.
    KEGGimmu:107885.
    UCSCiuc009qzk.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK004092 mRNA. Translation: BAB23165.1 .
    CT030686 , AC135634 Genomic DNA. Translation: CAQ52322.1 .
    CCDSi CCDS23394.1.
    RefSeqi NP_081105.1. NM_026829.2.
    UniGenei Mm.486274.

    3D structure databases

    ProteinModelPortali Q9D110.
    SMRi Q9D110. Positions 1-198.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 223657. 1 interaction.

    PTM databases

    PhosphoSitei Q9D110.

    Proteomic databases

    MaxQBi Q9D110.
    PaxDbi Q9D110.
    PRIDEi Q9D110.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000085256 ; ENSMUSP00000082354 ; ENSMUSG00000066442 .
    GeneIDi 107885.
    KEGGi mmu:107885.
    UCSCi uc009qzk.1. mouse.

    Organism-specific databases

    CTDi 10588.
    MGIi MGI:1340032. Mthfs.

    Phylogenomic databases

    eggNOGi COG0212.
    GeneTreei ENSGT00390000017791.
    HOGENOMi HOG000007299.
    HOVERGENi HBG052525.
    InParanoidi B2KF82.
    KOi K01934.
    OMAi VLEAPWF.
    PhylomeDBi Q9D110.
    TreeFami TF313668.

    Miscellaneous databases

    NextBioi 359643.
    PROi Q9D110.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9D110.
    Bgeei Q9D110.
    Genevestigatori Q9D110.

    Family and domain databases

    Gene3Di 3.40.50.10420. 1 hit.
    InterProi IPR002698. FTHF_cligase.
    IPR024185. FTHF_cligase-like.
    [Graphical view ]
    PANTHERi PTHR23407:SF1. PTHR23407:SF1. 1 hit.
    Pfami PF01812. 5-FTHF_cyc-lig. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006806. FTHF_cligase. 1 hit.
    TIGRFAMsi TIGR02727. MTHFS_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.

    Entry informationi

    Entry nameiMTHFS_MOUSE
    AccessioniPrimary (citable) accession number: Q9D110
    Secondary accession number(s): B2KF82
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 100 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3