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Protein

5-formyltetrahydrofolate cyclo-ligase

Gene

Mthfs

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-CHO-H4PteGlu) to yield 5,10-methenyltetrahydrofolate (By similarity).By similarity

Catalytic activityi

ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14ATPBy similarity1
Binding sitei56Substrate; via carbonyl oxygenBy similarity1
Binding sitei61SubstrateBy similarity1
Metal bindingi154MagnesiumBy similarity1
Metal bindingi189MagnesiumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 14ATPBy similarity5
Nucleotide bindingi145 – 153ATPBy similarity9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Folate-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.3.2 3474
ReactomeiR-MMU-196757 Metabolism of folate and pterines

Names & Taxonomyi

Protein namesi
Recommended name:
5-formyltetrahydrofolate cyclo-ligase (EC:6.3.3.2)
Alternative name(s):
5,10-methenyl-tetrahydrofolate synthetase
Short name:
MTHFS
Short name:
Methenyl-THF synthetase
Gene namesi
Name:Mthfs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1340032 Mthfs

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002002762 – 2035-formyltetrahydrofolate cyclo-ligaseAdd BLAST202

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9D110
PaxDbiQ9D110
PeptideAtlasiQ9D110
PRIDEiQ9D110

PTM databases

iPTMnetiQ9D110
PhosphoSitePlusiQ9D110

Expressioni

Gene expression databases

BgeeiENSMUSG00000066442
ExpressionAtlasiQ9D110 baseline and differential
GenevisibleiQ9D110 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi223657, 2 interactors
STRINGi10090.ENSMUSP00000082354

Structurei

3D structure databases

ProteinModelPortaliQ9D110
SMRiQ9D110
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni148 – 152Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3093 Eukaryota
COG0212 LUCA
GeneTreeiENSGT00390000017791
HOGENOMiHOG000007299
HOVERGENiHBG052525
InParanoidiQ9D110
KOiK01934
OMAiSTIYPCQ
OrthoDBiEOG091G0Q5W
PhylomeDBiQ9D110
TreeFamiTF313668

Family and domain databases

Gene3Di3.40.50.10420, 1 hit
InterProiView protein in InterPro
IPR002698 FTHF_cligase
IPR024185 FTHF_cligase-like_sf
IPR037171 NagB/RpiA_transferase-like
PANTHERiPTHR23407:SF1 PTHR23407:SF1, 1 hit
PfamiView protein in Pfam
PF01812 5-FTHF_cyc-lig, 1 hit
PIRSFiPIRSF006806 FTHF_cligase, 1 hit
SUPFAMiSSF100950 SSF100950, 1 hit
TIGRFAMsiTIGR02727 MTHFS_bact, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D110-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVTVNSAK RGLRAELKQR LRALSAEERL RQSLLLTQKV IAHNQYQNSK
60 70 80 90 100
RISIFLSMQD EVETEVIIKD IFKQGKICFI PRYQFQSNHM DMVRLTSSEE
110 120 130 140 150
IALLPKTSWN IHQPGEGDVR EEALSTGGLD LIFLPGLGFD KDGNRLGRGK
160 170 180 190 200
GYYDTYLKRC VQHQEVKPYT MALAFKEQIC PQIPVDEHDM KVDEVLYEDS

PAS
Length:203
Mass (Da):23,202
Last modified:January 23, 2007 - v2
Checksum:i4C20DC7416CA5304
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004092 mRNA Translation: BAB23165.1
CT030686, AC135634 Genomic DNA Translation: CAQ52322.1
CCDSiCCDS23394.1
RefSeqiNP_081105.1, NM_026829.2
UniGeneiMm.486274

Genome annotation databases

EnsembliENSMUST00000085256; ENSMUSP00000082354; ENSMUSG00000066442
GeneIDi107885
KEGGimmu:107885
UCSCiuc009qzk.1 mouse

Similar proteinsi

Entry informationi

Entry nameiMTHFS_MOUSE
AccessioniPrimary (citable) accession number: Q9D110
Secondary accession number(s): B2KF82
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health