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Protein

Pepsin A-5

Gene

Pga5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent (By similarity). May play a role as a specialized neonatal digestive enzyme (Probable).By similarity1 Publication

Catalytic activityi

Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.1 Publication

Enzyme regulationi

Inhibited by pepstatin A.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei92 – 921PROSITE-ProRule annotation
Active sitei275 – 2751PROSITE-ProRule annotation

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: MGI

GO - Biological processi

  • protein catabolic process Source: GO_Central
  • protein metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

ReactomeiR-MMU-5683826. Surfactant metabolism.

Protein family/group databases

MEROPSiA01.051.

Names & Taxonomyi

Protein namesi
Recommended name:
Pepsin A-5Imported (EC:3.4.23.11 Publication)
Alternative name(s):
Pepsin F1 Publication
Gene namesi
Name:Pga5Imported
Synonyms:Pepf1 Publication
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1915935. Pga5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515Sequence analysisAdd
BLAST
Propeptidei16 – 6247Activation peptideBy similarityPRO_0000436776Add
BLAST
Chaini63 – 387325Pepsin A-5CuratedPRO_0000436777Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi105 ↔ 110By similarity
Disulfide bondi266 ↔ 270By similarity
Disulfide bondi309 ↔ 343PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiQ9JKE6.

Expressioni

Tissue specificityi

Expressed in glandular chief cells of the neonatal stomach. Expressed in yolk sacs of the placenta (at protein level).1 Publication

Developmental stagei

In neonatal stomach, highly expressed for the first two weeks after birth, with rapidly decreasing expression after 17.5 days. In placenta, detected from 11.5 dpc until term.1 Publication

Gene expression databases

BgeeiENSMUSG00000024738.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025647.

Structurei

3D structure databases

SMRiQ9D106. Positions 16-387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini74 – 384311Peptidase A1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase A1 family.PROSITE-ProRule annotation
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
KOiK06002.
OMAiKCDTINT.
OrthoDBiEOG091G0JP7.
PhylomeDBiQ9D106.
TreeFamiTF314990.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 1 hit.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D106-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKWLWVLGLV ALSECLVKIP LMKIKSMREN LRESQVLKDY LEKYPRSRAH
60 70 80 90 100
VLLEQRRNPA VTYEPMRNYL DLVYIGIISI GTPPQEFRVV LDTGSSVLWV
110 120 130 140 150
PSIYCSSPAC AHHKAFNPLR SSTFLVSGRP VNVAYGSGEM SGFLAYDTVR
160 170 180 190 200
IGDLTVVAQA FGLSLEEPGI FMEYAVFDGI LGLGYPNLGL QGITPVFDNL
210 220 230 240 250
WLQGLIPQNL FAFYLSSKDE KGSMLMLGGV DPSYYHGELH WVPVSKPSYW
260 270 280 290 300
QLAVDSISMN GEVIACDGGC QGIMDTGTSL LTGPRSSIVN IQNLIGAKAS
310 320 330 340 350
GDGEYFLKCD TINTLPDIVF TIGSVTYPVP ASAYIRKDRS HNCRSNFEEG
360 370 380
MDDPSDPEMW VLGDVFLRLY FTVFDRANNR IGLAPAA
Length:387
Mass (Da):42,824
Last modified:June 1, 2001 - v1
Checksum:i177E12140ED5A0F7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti176 – 1761V → G in AAF61241 (PubMed:11566730).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF240776 mRNA. Translation: AAF61241.1.
AK004109 mRNA. Translation: BAB23174.1.
AK053965 mRNA. Translation: BAC35604.1.
AK168924 mRNA. Translation: BAE40737.1.
AC132247 Genomic DNA. No translation available.
BC119523 mRNA. Translation: AAI19524.1.
RefSeqiNP_067428.2. NM_021453.4.
UniGeneiMm.34037.

Genome annotation databases

EnsembliENSMUST00000025647; ENSMUSP00000025647; ENSMUSG00000024738.
GeneIDi58803.
KEGGimmu:58803.
UCSCiuc008gqp.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF240776 mRNA. Translation: AAF61241.1.
AK004109 mRNA. Translation: BAB23174.1.
AK053965 mRNA. Translation: BAC35604.1.
AK168924 mRNA. Translation: BAE40737.1.
AC132247 Genomic DNA. No translation available.
BC119523 mRNA. Translation: AAI19524.1.
RefSeqiNP_067428.2. NM_021453.4.
UniGeneiMm.34037.

3D structure databases

SMRiQ9D106. Positions 16-387.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025647.

Protein family/group databases

MEROPSiA01.051.

Proteomic databases

PaxDbiQ9JKE6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025647; ENSMUSP00000025647; ENSMUSG00000024738.
GeneIDi58803.
KEGGimmu:58803.
UCSCiuc008gqp.2. mouse.

Organism-specific databases

CTDi5222.
MGIiMGI:1915935. Pga5.

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
KOiK06002.
OMAiKCDTINT.
OrthoDBiEOG091G0JP7.
PhylomeDBiQ9D106.
TreeFamiTF314990.

Enzyme and pathway databases

ReactomeiR-MMU-5683826. Surfactant metabolism.

Miscellaneous databases

SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024738.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 1 hit.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEPA5_MOUSE
AccessioniPrimary (citable) accession number: Q9D106
Secondary accession number(s): Q9JKE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 6, 2016
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.