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Protein

Pepsin A-5

Gene

Pga5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent (By similarity). May play a role as a specialized neonatal digestive enzyme (Probable).By similarity1 Publication

Catalytic activityi

Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.1 Publication

Enzyme regulationi

Inhibited by pepstatin A.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei92PROSITE-ProRule annotation1
Active sitei275PROSITE-ProRule annotation1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: MGI

GO - Biological processi

  • autophagy Source: GO_Central
  • protein catabolic process Source: GO_Central
  • protein metabolic process Source: MGI

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

ReactomeiR-MMU-5683826 Surfactant metabolism

Protein family/group databases

MEROPSiA01.051

Names & Taxonomyi

Protein namesi
Recommended name:
Pepsin A-5Imported (EC:3.4.23.11 Publication)
Alternative name(s):
Pepsin F1 Publication
Gene namesi
Name:Pga5Imported
Synonyms:Pepf1 Publication
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1915935 Pga5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Sequence analysisAdd BLAST15
PropeptideiPRO_000043677616 – 62Activation peptideBy similarityAdd BLAST47
ChainiPRO_000043677763 – 387Pepsin A-5CuratedAdd BLAST325

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi105 ↔ 110By similarity
Disulfide bondi266 ↔ 270By similarity
Disulfide bondi309 ↔ 343PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiQ9D106
PRIDEiQ9D106

PTM databases

PhosphoSitePlusiQ9D106

Expressioni

Tissue specificityi

Expressed in glandular chief cells of the neonatal stomach. Expressed in yolk sacs of the placenta (at protein level).1 Publication

Developmental stagei

In neonatal stomach, highly expressed for the first two weeks after birth, with rapidly decreasing expression after 17.5 days. In placenta, detected from 11.5 dpc until term.1 Publication

Gene expression databases

BgeeiENSMUSG00000024738

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025647

Structurei

3D structure databases

ProteinModelPortaliQ9D106
SMRiQ9D106
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 384Peptidase A1PROSITE-ProRule annotationAdd BLAST311

Sequence similaritiesi

Belongs to the peptidase A1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339 Eukaryota
ENOG410XNV7 LUCA
GeneTreeiENSGT00760000118929
HOGENOMiHOG000197681
HOVERGENiHBG000482
KOiK06002
OMAiKCDTINT
OrthoDBiEOG091G0JP7
PhylomeDBiQ9D106
TreeFamiTF314990

Family and domain databases

Gene3Di2.40.70.10, 2 hits
InterProiView protein in InterPro
IPR001461 Aspartic_peptidase_A1
IPR001969 Aspartic_peptidase_AS
IPR012848 Aspartic_peptidase_N
IPR033121 PEPTIDASE_A1
IPR021109 Peptidase_aspartic_dom_sf
PANTHERiPTHR13683 PTHR13683, 1 hit
PfamiView protein in Pfam
PF07966 A1_Propeptide, 1 hit
PF00026 Asp, 1 hit
PRINTSiPR00792 PEPSIN
SUPFAMiSSF50630 SSF50630, 1 hit
PROSITEiView protein in PROSITE
PS00141 ASP_PROTEASE, 1 hit
PS51767 PEPTIDASE_A1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D106-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKWLWVLGLV ALSECLVKIP LMKIKSMREN LRESQVLKDY LEKYPRSRAH
60 70 80 90 100
VLLEQRRNPA VTYEPMRNYL DLVYIGIISI GTPPQEFRVV LDTGSSVLWV
110 120 130 140 150
PSIYCSSPAC AHHKAFNPLR SSTFLVSGRP VNVAYGSGEM SGFLAYDTVR
160 170 180 190 200
IGDLTVVAQA FGLSLEEPGI FMEYAVFDGI LGLGYPNLGL QGITPVFDNL
210 220 230 240 250
WLQGLIPQNL FAFYLSSKDE KGSMLMLGGV DPSYYHGELH WVPVSKPSYW
260 270 280 290 300
QLAVDSISMN GEVIACDGGC QGIMDTGTSL LTGPRSSIVN IQNLIGAKAS
310 320 330 340 350
GDGEYFLKCD TINTLPDIVF TIGSVTYPVP ASAYIRKDRS HNCRSNFEEG
360 370 380
MDDPSDPEMW VLGDVFLRLY FTVFDRANNR IGLAPAA
Length:387
Mass (Da):42,824
Last modified:June 1, 2001 - v1
Checksum:i177E12140ED5A0F7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti176V → G in AAF61241 (PubMed:11566730).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF240776 mRNA Translation: AAF61241.1
AK004109 mRNA Translation: BAB23174.1
AK053965 mRNA Translation: BAC35604.1
AK168924 mRNA Translation: BAE40737.1
AC132247 Genomic DNA No translation available.
BC119523 mRNA Translation: AAI19524.1
CCDSiCCDS29584.1
RefSeqiNP_067428.2, NM_021453.4
UniGeneiMm.34037

Genome annotation databases

EnsembliENSMUST00000025647; ENSMUSP00000025647; ENSMUSG00000024738
GeneIDi58803
KEGGimmu:58803
UCSCiuc008gqp.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPEPA5_MOUSE
AccessioniPrimary (citable) accession number: Q9D106
Secondary accession number(s): Q9JKE6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 6, 2016
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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