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Q9D103 (IFM1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interferon-induced transmembrane protein 1
Alternative name(s):
Dispanin subfamily A member 2a
Short name=DSPA2a
Fragilis protein 2
Mouse ifitm-like protein 2
Short name=Mil-2
Gene names
Name:Ifitm1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length106 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV), Ebola virus (EBOV), Dengue virus (DNV) and West Nile virus (WNV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry and SARS-CoV S protein-mediated viral entry. Also implicated in cell adhesion and control of cell growth and migration. Plays a key role in the antiproliferative action of IFN-gamma either by inhibiting the ERK activation or by arresting cell growth in G1 phase in a p53-dependent manner. Acts as a positive regulator of osteoblast differentiation. Ref.7 Ref.9

Subunit structure

Interacts with CAV1; this interaction enhances the ability of CAV1 in inhibiting ERK activation By similarity. Interacts with CD81 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.6.

Tissue specificity

Predominantly expressed in nascent primordial germ cells, as well as in gonadal germ cells. Ref.5

Developmental stage

In migrating PGCs, expression is first detected during germ cell differentiation. Ref.1 Ref.6

Sequence similarities

Belongs to the CD225/Dispanin family.

Caution

It has been previously shown that mediates migration of early primordial germ cells (PGCs) (Ref.6). But according to Ref.6, have no detectable effects on development of the germ line or on the generation of live young, hence, is not essential for PGC migration.

Ontologies

Keywords
   Biological processAntiviral defense
Immunity
Innate immunity
Osteogenesis
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   PTMLipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanterior/posterior pattern specification

Inferred from mutant phenotype PubMed 15857914. Source: MGI

defense response to virus

Inferred from direct assay PubMed 20064371. Source: MGI

negative regulation of cell migration

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of viral entry into host cell

Inferred from direct assay Ref.9. Source: UniProtKB

negative regulation of viral genome replication

Inferred from sequence or structural similarity. Source: UniProtKB

ossification

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of osteoblast differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

response to interferon-alpha

Inferred from sequence or structural similarity. Source: UniProtKB

response to interferon-beta

Inferred from sequence or structural similarity. Source: UniProtKB

response to interferon-gamma

Inferred from sequence or structural similarity. Source: UniProtKB

response to virus

Inferred from direct assay Ref.9. Source: UniProtKB

somitogenesis

Inferred from mutant phenotype PubMed 15857914. Source: MGI

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 106106Interferon-induced transmembrane protein 1
PRO_0000398565

Regions

Topological domain1 – 3535Extracellular Potential
Transmembrane36 – 5621Helical; Potential
Topological domain57 – 8428Cytoplasmic Potential
Transmembrane85 – 10521Helical; Potential
Topological domain1061Extracellular Potential

Amino acid modifications

Lipidation491S-palmitoyl cysteine By similarity
Lipidation501S-palmitoyl cysteine By similarity
Lipidation831S-palmitoyl cysteine By similarity

Experimental info

Sequence conflict371V → I in AAH27285. Ref.3
Sequence conflict541V → I in AAH27285. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9D103 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 7B0E7D8200D36631

FASTA10611,524
        10         20         30         40         50         60 
MPKEQQEVVV LGSPHISTSA TATTINMPEI STPDHVVWSL FNTLFMNFCC LGFVAYAYSV 

        70         80         90        100 
KSRDRKMVGD TTGAQAFAST AKCLNISSLF FTILTAIVVI VVCAIR 

« Hide

References

« Hide 'large scale' references
[1]"Developmentally regulated expression of mil-1 and mil-2, mouse interferon-induced transmembrane protein like genes, during formation and differentiation of primordial germ cells."
Tanaka S.S., Matsui Y.
Mech. Dev. 119:S261-S267(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Embryo.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II and FVB/N.
Tissue: Colon, Heart, Lung and Mammary tumor.
[5]"The fragilis interferon-inducible gene family of transmembrane proteins is associated with germ cell specification in mice."
Lange U.C., Saitou M., Western P.S., Barton S.C., Surani M.A.
BMC Dev. Biol. 3:1-1(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[6]"IFITM/Mil/fragilis family proteins IFITM1 and IFITM3 play distinct roles in mouse primordial germ cell homing and repulsion."
Tanaka S.S., Yamaguchi Y.L., Tsoi B., Lickert H., Tam P.P.
Dev. Cell 9:745-756(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[7]"Normal germ line establishment in mice carrying a deletion of the Ifitm/Fragilis gene family cluster."
Lange U.C., Adams D.J., Lee C., Barton S., Schneider R., Bradley A., Surani M.A.
Mol. Cell. Biol. 28:4688-4696(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"The small interferon-induced transmembrane genes and proteins."
Siegrist F., Ebeling M., Certa U.
J. Interferon Cytokine Res. 31:183-197(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[9]"Distinct patterns of IFITM-mediated restriction of filoviruses, SARS coronavirus, and influenza A virus."
Huang I.C., Bailey C.C., Weyer J.L., Radoshitzky S.R., Becker M.M., Chiang J.J., Brass A.L., Ahmed A.A., Chi X., Dong L., Longobardi L.E., Boltz D., Kuhn J.H., Elledge S.J., Bavari S., Denison M.R., Choe H., Farzan M.
PLoS Pathog. 7:E1001258-E1001258(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"The dispanins: a novel gene family of ancient origin that contains 14 human members."
Sallman Almen M., Bringeland N., Fredriksson R., Schioth H.B.
PLoS ONE 7:E31961-E31961(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK004121 mRNA. Translation: BAB23181.1.
AK169960 mRNA. Translation: BAE41483.1.
CH466531 Genomic DNA. Translation: EDL17976.1.
BC027285 mRNA. Translation: AAH27285.1.
BC090258 mRNA. Translation: AAH90258.1.
BC090972 mRNA. Translation: AAH90972.1.
BK001123 mRNA. Translation: DAA01238.1.
CCDSCCDS21995.1.
RefSeqNP_001106186.1. NM_001112715.1.
NP_081096.3. NM_026820.3.
XP_006536301.1. XM_006536238.1.
UniGeneMm.175661.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000101657.

Proteomic databases

PRIDEQ9D103.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026564; ENSMUSP00000026564; ENSMUSG00000025491.
ENSMUST00000106040; ENSMUSP00000101655; ENSMUSG00000025491.
ENSMUST00000106042; ENSMUSP00000101657; ENSMUSG00000025491.
GeneID68713.
KEGGmmu:68713.
UCSCuc009kja.2. mouse.

Organism-specific databases

CTD8519.
MGIMGI:1915963. Ifitm1.

Phylogenomic databases

eggNOGspriNOG13868.
GeneTreeENSGT00390000003476.
HOGENOMHOG000115781.
HOVERGENHBG001182.
InParanoidQ9D103.
KOK06566.
OMATITEITI.
OrthoDBEOG7288T5.
PhylomeDBQ9D103.
TreeFamTF334894.

Gene expression databases

BgeeQ9D103.
GenevestigatorQ9D103.

Family and domain databases

InterProIPR007593. CD225/Dispanin_fam.
[Graphical view]
PfamPF04505. Dispanin. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio327746.
PROQ9D103.
SOURCESearch...

Entry information

Entry nameIFM1_MOUSE
AccessionPrimary (citable) accession number: Q9D103
Secondary accession number(s): Q8R2S7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot