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Protein

Dual specificity protein phosphatase 12

Gene

Dusp12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Dual specificity phosphatase; can dephosphorylate both phosphotyrosine and phosphoserine or phosphothreonine residues. Can dephosphorylate glucokinase (in vitro). Has phosphatase activity with the synthetic substrate 6,8-difluoro-4-methylumbelliferyl phosphate and other in vitro substrates.By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.By similarity
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.By similarity

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei114Phosphocysteine intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 12 (EC:3.1.3.16By similarity, EC:3.1.3.48By similarity)
Alternative name(s):
Dual specificity phosphatase T-DSP4
Dual specificity phosphatase VH1
Gene namesi
Name:Dusp12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1890614 Dusp12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000948191 – 339Dual specificity protein phosphatase 12Add BLAST339

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei334PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9D0T2
MaxQBiQ9D0T2
PaxDbiQ9D0T2
PRIDEiQ9D0T2

PTM databases

PhosphoSitePlusiQ9D0T2

Expressioni

Gene expression databases

BgeeiENSMUSG00000026659
CleanExiMM_DUSP12
ExpressionAtlasiQ9D0T2 baseline and differential
GenevisibleiQ9D0T2 MM

Interactioni

Subunit structurei

Monomer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027970

Structurei

3D structure databases

ProteinModelPortaliQ9D0T2
SMRiQ9D0T2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini87 – 161Tyrosine-protein phosphataseAdd BLAST75

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni115 – 120Substrate bindingBy similarity6

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1716 Eukaryota
COG2453 LUCA
GeneTreeiENSGT00760000118853
HOGENOMiHOG000243638
HOVERGENiHBG051421
InParanoidiQ9D0T2
KOiK14819
OMAiQGRLYCP
OrthoDBiEOG091G0QP2
PhylomeDBiQ9D0T2
TreeFamiTF105123

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR016278 DUSP12
IPR029021 Prot-tyrosine_phosphatase-like
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
PIRSFiPIRSF000941 DUSP12, 1 hit
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit

Sequencei

Sequence statusi: Complete.

Q9D0T2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEAQGSNHG CERQAPTASP ASSAGHAVEV RPGLYLGGAA AVAEPGHLRE
60 70 80 90 100
AGITAVLTVD SEPAFPAGAG FEGLRSLFVP ALDKPETDLL SHLDRCVAFI
110 120 130 140 150
GQARSEGRAV LVHCHAGVSR SVAVVMAFIM KTDQLTFEKA YDILRTVKPE
160 170 180 190 200
AKVNEGFEWQ LKLYEAMGYE VDTSSAFYKQ YRLQKVTEKC PKLWNLPQEL
210 220 230 240 250
FAVDPTTISQ GLKDDILYKC RKCRRSLFRH SSILGHSEGS GPIAFAHKRT
260 270 280 290 300
APSSVLTTGS QAQCTSYFIE PVQWMESTLL GVMDGQLLCP KCSAKLGSFN
310 320 330
WYGEQCSCGR WITPAFQIHK NRVDEMKMLP VLGSQTKKL
Length:339
Mass (Da):37,159
Last modified:June 1, 2001 - v1
Checksum:iB6A254D319BEC98F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7S → T in AAG44739 (PubMed:11130991).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF280810 mRNA Translation: AAK69508.1
AF268196 mRNA Translation: AAG44739.1
AK004488 mRNA Translation: BAB23328.1
CCDSiCCDS15474.1
RefSeqiNP_075662.2, NM_023173.2
UniGeneiMm.34365

Genome annotation databases

EnsembliENSMUST00000027970; ENSMUSP00000027970; ENSMUSG00000026659
GeneIDi80915
KEGGimmu:80915
UCSCiuc007dml.1 mouse

Similar proteinsi

Entry informationi

Entry nameiDUS12_MOUSE
AccessioniPrimary (citable) accession number: Q9D0T2
Secondary accession number(s): Q9EQD3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: June 1, 2001
Last modified: February 28, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health