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Protein

Dual specificity protein phosphatase 12

Gene

Dusp12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Dual specificity phosphatase; can dephosphorylate both phosphotyrosine and phosphoserine or phosphothreonine residues. Can dephosphorylate glucokinase (in vitro). Has phosphatase activity with the synthetic substrate 6,8-difluoro-4-methylumbelliferyl phosphate and other in vitro substrates.By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.By similarity
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.By similarity

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei114Phosphocysteine intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 12 (EC:3.1.3.16By similarity, EC:3.1.3.48By similarity)
Alternative name(s):
Dual specificity phosphatase T-DSP4
Dual specificity phosphatase VH1
Gene namesi
Name:Dusp12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1890614. Dusp12.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000948191 – 339Dual specificity protein phosphatase 12Add BLAST339

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei334PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9D0T2.
MaxQBiQ9D0T2.
PaxDbiQ9D0T2.
PRIDEiQ9D0T2.

PTM databases

PhosphoSitePlusiQ9D0T2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026659.
CleanExiMM_DUSP12.
ExpressionAtlasiQ9D0T2. baseline and differential.
GenevisibleiQ9D0T2. MM.

Interactioni

Subunit structurei

Monomer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027970.

Structurei

3D structure databases

ProteinModelPortaliQ9D0T2.
SMRiQ9D0T2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini87 – 161Tyrosine-protein phosphataseAdd BLAST75

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni115 – 120Substrate bindingBy similarity6

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1716. Eukaryota.
COG2453. LUCA.
GeneTreeiENSGT00890000139517.
HOGENOMiHOG000243638.
HOVERGENiHBG051421.
InParanoidiQ9D0T2.
KOiK14819.
OMAiQGRLYCP.
OrthoDBiEOG091G0QP2.
PhylomeDBiQ9D0T2.
TreeFamiTF105123.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di3.90.190.10. 1 hit.
InterProiView protein in InterPro
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR016278. DUSP12.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiView protein in Pfam
PF00782. DSPc. 1 hit.
PIRSFiPIRSF000941. DUSP12. 1 hit.
SMARTiView protein in SMART
SM00195. DSPc. 1 hit.
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiView protein in PROSITE
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9D0T2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEAQGSNHG CERQAPTASP ASSAGHAVEV RPGLYLGGAA AVAEPGHLRE
60 70 80 90 100
AGITAVLTVD SEPAFPAGAG FEGLRSLFVP ALDKPETDLL SHLDRCVAFI
110 120 130 140 150
GQARSEGRAV LVHCHAGVSR SVAVVMAFIM KTDQLTFEKA YDILRTVKPE
160 170 180 190 200
AKVNEGFEWQ LKLYEAMGYE VDTSSAFYKQ YRLQKVTEKC PKLWNLPQEL
210 220 230 240 250
FAVDPTTISQ GLKDDILYKC RKCRRSLFRH SSILGHSEGS GPIAFAHKRT
260 270 280 290 300
APSSVLTTGS QAQCTSYFIE PVQWMESTLL GVMDGQLLCP KCSAKLGSFN
310 320 330
WYGEQCSCGR WITPAFQIHK NRVDEMKMLP VLGSQTKKL
Length:339
Mass (Da):37,159
Last modified:June 1, 2001 - v1
Checksum:iB6A254D319BEC98F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7S → T in AAG44739 (PubMed:11130991).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF280810 mRNA. Translation: AAK69508.1.
AF268196 mRNA. Translation: AAG44739.1.
AK004488 mRNA. Translation: BAB23328.1.
CCDSiCCDS15474.1.
RefSeqiNP_075662.2. NM_023173.2.
UniGeneiMm.34365.

Genome annotation databases

EnsembliENSMUST00000027970; ENSMUSP00000027970; ENSMUSG00000026659.
GeneIDi80915.
KEGGimmu:80915.
UCSCiuc007dml.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiDUS12_MOUSE
AccessioniPrimary (citable) accession number: Q9D0T2
Secondary accession number(s): Q9EQD3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: June 1, 2001
Last modified: August 30, 2017
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families