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Protein

NHP2-like protein 1

Gene

Snu13

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing as component of the spliceosome. Binds to the 5'-stem-loop of U4 snRNA and thereby contributes to spliceosome assembly. The protein undergoes a conformational change upon RNA-binding.By similarity

GO - Molecular functioni

  • ATPase binding Source: MGI
  • box C/D snoRNA binding Source: CAFA
  • U3 snoRNA binding Source: MGI
  • U4atac snRNA binding Source: MGI
  • U4 snRNA binding Source: MGI

GO - Biological processi

  • box C/D snoRNP assembly Source: CAFA
  • maturation of LSU-rRNA Source: GO_Central
  • maturation of SSU-rRNA Source: GO_Central
  • mRNA splicing, via spliceosome Source: GO_Central
  • single fertilization Source: MGI

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72165. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
NHP2-like protein 1
Alternative name(s):
Fertilization antigen 11 Publication
Short name:
FA-1
High mobility group-like nuclear protein 2 homolog 1
Sperm-specific antigen 1
U4/U6.U5 small nuclear ribonucleoprotein SNU13By similarity
U4/U6.U5 tri-snRNP 15.5 kDa protein
Cleaved into the following chain:
Gene namesi
Name:Snu13By similarity
Synonyms:Nhp2l1Imported, Ssfa1
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:893586. Snu13.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001367791 – 128NHP2-like protein 1Add BLAST128
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00004232622 – 128NHP2-like protein 1, N-terminally processedAdd BLAST127

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylthreonine; in NHP2-like protein 1, N-terminally processedBy similarity1
Modified residuei21N6-acetyllysineCombined sources1
Modified residuei122PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9D0T1.
MaxQBiQ9D0T1.
PaxDbiQ9D0T1.
PeptideAtlasiQ9D0T1.
PRIDEiQ9D0T1.
TopDownProteomicsiQ9D0T1.

PTM databases

iPTMnetiQ9D0T1.
PhosphoSitePlusiQ9D0T1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000063480.
ExpressionAtlasiQ9D0T1. baseline and differential.
GenevisibleiQ9D0T1. MM.

Interactioni

Subunit structurei

Identified in the spliceosome B complex. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, WDR57, SNRNP40, DDX23, CD2BP2, PPIH, NHP2L1, EFTUD2, SART1 and USP39. Interacts with RAD17 and PRPF31. The complex formed by SNU13 and PRPF31 binds U4 snRNA. The complex formed by SNU13 and PRPF31 binds also U4atac snRNA, a characteristic component of specific, less abundant spliceosomal complexes.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei61Interaction with U4 snRNA and U4atac snRNABy similarity1
Sitei86Interaction with U4 snRNA and U4atac snRNABy similarity1

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203509. 48 interactors.
IntActiQ9D0T1. 52 interactors.
MINTiQ9D0T1.
STRINGi10090.ENSMUSP00000130867.

Structurei

3D structure databases

ProteinModelPortaliQ9D0T1.
SMRiQ9D0T1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni36 – 48Interaction with U4 snRNA and U4atac snRNABy similarityAdd BLAST13
Regioni96 – 128Important for U4 snRNA-bindingBy similarityAdd BLAST33

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3387. Eukaryota.
COG1358. LUCA.
GeneTreeiENSGT00550000074840.
HOGENOMiHOG000055226.
HOVERGENiHBG000328.
InParanoidiQ9D0T1.
KOiK12845.
OMAiKPFYVRF.
OrthoDBiEOG091G0YVI.
PhylomeDBiQ9D0T1.
TreeFamiTF300184.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
InterProiView protein in InterPro
IPR002415. H/ACA_rnp_Nhp2_euk.
IPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR004037. Ribosomal_L7Ae_CS.
PfamiView protein in Pfam
PF01248. Ribosomal_L7Ae. 1 hit.
PRINTSiPR00881. L7ARS6FAMILY.
PR00883. NUCLEARHMG.
SUPFAMiSSF55315. SSF55315. 1 hit.
PROSITEiView protein in PROSITE
PS01082. RIBOSOMAL_L7AE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D0T1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEADVNPKA YPLADAHLTK KLLDLVQQSC NYKQLRKGAN EATKTLNRGI
60 70 80 90 100
SEFIVMAADA EPLEIILHLP LLCEDKNVPY VFVRSKQALG RACGVSRPVI
110 120
ACSVTIKEGS QLKQQIQSIQ QSIERLLV
Length:128
Mass (Da):14,174
Last modified:January 23, 2007 - v4
Checksum:i78849EBB497089ED
GO

Sequence cautioni

The sequence AAC53177 differs from that shown. Reason: Frameshift at positions 11, 54, 70, 93 and 128.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9K → R in BAB23329 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95114 mRNA. Translation: AAC53177.1. Frameshift.
AK004489 mRNA. Translation: BAB23329.1.
AK134943 mRNA. Translation: BAE22349.1.
AK145608 mRNA. Translation: BAE26537.1.
AK168930 mRNA. Translation: BAE40743.1.
AK169103 mRNA. Translation: BAE40885.1.
BC026755 mRNA. Translation: AAH26755.1.
BC054450 mRNA. Translation: AAH54450.1.
BC083315 mRNA. Translation: AAH83315.1.
CCDSiCCDS37154.1.
PIRiT10105.
RefSeqiNP_035612.2. NM_011482.4.
XP_003689233.1. XM_003689185.5.
UniGeneiMm.299312.

Genome annotation databases

EnsembliENSMUST00000080622; ENSMUSP00000091840; ENSMUSG00000063480.
GeneIDi100862468.
20826.
KEGGimmu:100862468.
mmu:20826.
UCSCiuc007wya.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiNH2L1_MOUSE
AccessioniPrimary (citable) accession number: Q9D0T1
Secondary accession number(s): O08754, Q3UY64
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 140 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome