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Protein

Intraflagellar transport protein 27 homolog

Gene

Ift27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Small GTPase-like component of the intraflagellar transport (IFT) complex B that promotes the exit of the BBSome complex from cilia via its interaction with ARL6 (PubMed:25446516). Not involved in entry of the BBSome complex into cilium. Prevents aggregation of GTP-free ARL6. Required for hedgehog signaling (PubMed:25446516). Forms a subcomplex within the IFT complex B with IFT25 (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 198GTPBy similarity
Nucleotide bindingi64 – 685GTPBy similarity
Nucleotide bindingi123 – 1264GTPBy similarity

GO - Molecular functioni

GO - Biological processi

  • cochlea development Source: MGI
  • inner ear receptor stereocilium organization Source: MGI
  • intracellular protein transport Source: UniProtKB
  • intraciliary transport Source: UniProtKB
  • small GTPase mediated signal transduction Source: InterPro
  • smoothened signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5620924. Intraflagellar transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Intraflagellar transport protein 27 homolog
Alternative name(s):
Putative GTP-binding protein RAY-like
Rab-like protein 4
Gene namesi
Name:Ift27
Synonyms:Rabl4, Rayl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1914292. Ift27.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: MGI
  • cilium Source: MGI
  • intraciliary transport particle B Source: UniProtKB
  • primary cilium Source: Reactome
  • sperm midpiece Source: MGI
  • sperm principal piece Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium

Pathology & Biotechi

Disruption phenotypei

Null mutants retain the ability to ciliate and survive through gestation. They die shortly after birth due to different phenotypes reminiscent of Shh signaling defects: polydactyly, lung isomerisms, and structural heart defects.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 186186Intraflagellar transport protein 27 homologPRO_0000082716Add
BLAST

Proteomic databases

EPDiQ9D0P8.
MaxQBiQ9D0P8.
PaxDbiQ9D0P8.
PeptideAtlasiQ9D0P8.
PRIDEiQ9D0P8.

PTM databases

iPTMnetiQ9D0P8.
PhosphoSiteiQ9D0P8.

Expressioni

Tissue specificityi

Co-localizes with RABL2/RABL2A in the midpiece of elongated spermatids within the testis (at protein level).1 Publication

Gene expression databases

BgeeiQ9D0P8.
CleanExiMM_RABL4.
GenevisibleiQ9D0P8. MM.

Interactioni

Subunit structurei

Component of the IFT complex B, at least composed of IFT20, IFT22, HSPB11/IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74, IFT80, IFT81, and IFT88. Interacts with IFT25; the interaction is direct. Interacts with RABL2/RABL2A; binding is equal in the presence of GTP or GDP. Interacts with IFT88. Interacts with ARL6; recognizes and binds with the GTP-free form of ARL6 (By similarity).By similarity3 Publications

Protein-protein interaction databases

BioGridi211896. 3 interactions.
IntActiQ9D0P8. 7 interactions.
STRINGi10090.ENSMUSP00000016781.

Structurei

3D structure databases

ProteinModelPortaliQ9D0P8.
SMRiQ9D0P8. Positions 7-164.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0079. Eukaryota.
ENOG4111IWZ. LUCA.
GeneTreeiENSGT00830000128415.
HOVERGENiHBG105393.
InParanoidiQ9D0P8.
KOiK07934.
OMAiFHCLAKQ.
OrthoDBiEOG75J0PC.
PhylomeDBiQ9D0P8.
TreeFamiTF329292.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9D0P8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKLAAKCIL AGDPAVGKTA LVQMFRSDGT HFQKNYTLTT GVDLVVKTVP
60 70 80 90 100
VLDTNDSVEL FIFDSAGKEL FSEMLDKLWE NPNVLCLVYD VTNEQSFISC
110 120 130 140 150
TKWLEKVRSQ TSGISLPGVL VGTKTDLAGR QTVDSAQAQV WALSQGLEFF
160 170 180
ETSVKEMDNY EAPFHCLAKQ FYQLYREKVD IFHTLV
Length:186
Mass (Da):20,814
Last modified:June 1, 2001 - v1
Checksum:i1CFC788EF5592C51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011196 mRNA. No translation available.
BC017514 mRNA. Translation: AAH17514.1.
CCDSiCCDS37131.1.
RefSeqiNP_080207.1. NM_025931.2.
UniGeneiMm.30191.

Genome annotation databases

EnsembliENSMUST00000016781; ENSMUSP00000016781; ENSMUSG00000016637.
GeneIDi67042.
KEGGimmu:67042.
UCSCiuc007wor.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011196 mRNA. No translation available.
BC017514 mRNA. Translation: AAH17514.1.
CCDSiCCDS37131.1.
RefSeqiNP_080207.1. NM_025931.2.
UniGeneiMm.30191.

3D structure databases

ProteinModelPortaliQ9D0P8.
SMRiQ9D0P8. Positions 7-164.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211896. 3 interactions.
IntActiQ9D0P8. 7 interactions.
STRINGi10090.ENSMUSP00000016781.

PTM databases

iPTMnetiQ9D0P8.
PhosphoSiteiQ9D0P8.

Proteomic databases

EPDiQ9D0P8.
MaxQBiQ9D0P8.
PaxDbiQ9D0P8.
PeptideAtlasiQ9D0P8.
PRIDEiQ9D0P8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000016781; ENSMUSP00000016781; ENSMUSG00000016637.
GeneIDi67042.
KEGGimmu:67042.
UCSCiuc007wor.1. mouse.

Organism-specific databases

CTDi11020.
MGIiMGI:1914292. Ift27.

Phylogenomic databases

eggNOGiKOG0079. Eukaryota.
ENOG4111IWZ. LUCA.
GeneTreeiENSGT00830000128415.
HOVERGENiHBG105393.
InParanoidiQ9D0P8.
KOiK07934.
OMAiFHCLAKQ.
OrthoDBiEOG75J0PC.
PhylomeDBiQ9D0P8.
TreeFamiTF329292.

Enzyme and pathway databases

ReactomeiR-MMU-5620924. Intraflagellar transport.

Miscellaneous databases

PROiQ9D0P8.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D0P8.
CleanExiMM_RABL4.
GenevisibleiQ9D0P8. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Retina.
  3. Cited for: IDENTIFICATION IN THE IFT COMPLEX B, SUBCELLULAR LOCATION, INTERACTION WITH ITF88.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.
  5. "IFT25 links the signal-dependent movement of Hedgehog components to intraflagellar transport."
    Keady B.T., Samtani R., Tobita K., Tsuchya M., San Agustin J.T., Follit J.A., Jonassen J.A., Subramanian R., Lo C.W., Pazour G.J.
    Dev. Cell 22:940-951(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH IFT25.
  6. "RAB-like 2 has an essential role in male fertility, sperm intra-flagellar transport, and tail assembly."
    Lo J.C., Jamsai D., O'Connor A.E., Borg C., Clark B.J., Whisstock J.C., Field M.C., Adams V., Ishikawa T., Aitken R.J., Whittle B., Goodnow C.C., Ormandy C.J., O'Bryan M.K.
    PLoS Genet. 8:E1002969-E1002969(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RABL2, TISSUE SPECIFICITY.
  7. Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiIFT27_MOUSE
AccessioniPrimary (citable) accession number: Q9D0P8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.