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Protein

Chromatin assembly factor 1 subunit B

Gene

Chaf1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, DNA damage, DNA repair, DNA replication, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Chromatin assembly factor 1 subunit B
Short name:
CAF-1 subunit B
Alternative name(s):
Chromatin assembly factor I p60 subunit
Short name:
CAF-I 60 kDa subunit
Short name:
CAF-I p60
Gene namesi
Name:Chaf1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1314881. Chaf1b.

Subcellular locationi

  • Nucleus By similarity

  • Note: DNA replication foci.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508971 – 572Chromatin assembly factor 1 subunit BAdd BLAST572

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei401PhosphothreonineBy similarity1
Modified residuei416PhosphoserineBy similarity1
Modified residuei426PhosphothreonineBy similarity1
Modified residuei436PhosphoserineCombined sources1
Modified residuei440PhosphothreonineBy similarity1
Modified residuei456PhosphoserineCombined sources1
Modified residuei465PhosphoserineBy similarity1
Modified residuei501N6-acetyllysineBy similarity1
Modified residuei502PhosphothreonineSequence analysis1
Modified residuei510PhosphothreonineSequence analysis1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9D0N7.
MaxQBiQ9D0N7.
PaxDbiQ9D0N7.
PeptideAtlasiQ9D0N7.
PRIDEiQ9D0N7.

PTM databases

iPTMnetiQ9D0N7.
PhosphoSitePlusiQ9D0N7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022945.
CleanExiMM_CHAF1B.
ExpressionAtlasiQ9D0N7. baseline and differential.
GenevisibleiQ9D0N7. MM.

Interactioni

Subunit structurei

Subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi225873. 2 interactors.
IntActiQ9D0N7. 2 interactors.
MINTiMINT-4614962.
STRINGi10090.ENSMUSP00000023666.

Structurei

3D structure databases

ProteinModelPortaliQ9D0N7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati11 – 54WD 1Add BLAST44
Repeati64 – 103WD 2Add BLAST40
Repeati127 – 166WD 3Add BLAST40
Repeati169 – 208WD 4Add BLAST40
Repeati228 – 279WD 5Add BLAST52
Repeati301 – 347WD 6Add BLAST47
Repeati351 – 392WD 7Add BLAST42

Sequence similaritiesi

Belongs to the WD repeat HIR1 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1009. Eukaryota.
ENOG410XPU4. LUCA.
GeneTreeiENSGT00550000074968.
HOGENOMiHOG000183312.
HOVERGENiHBG050780.
InParanoidiQ9D0N7.
KOiK10751.
OMAiQDGYCTL.
OrthoDBiEOG091G0FIG.
PhylomeDBiQ9D0N7.
TreeFamiTF313062.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR029129. CAF1_p60_C.
IPR001632. Gprotein_B.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF15512. CAF-1_p60_C. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9D0N7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVITCEIAW HNKEPVYSLD FQHGATWKIH RLASAGVDTA VRIWKLERGP
60 70 80 90 100
DGKAIVEFLS NLARHTKAVN VVRFSPTGEI LASGGDDAVI LLWKMNDSKE
110 120 130 140 150
PEQIAFQDEE EAQLNKENWT VVKTLRGHLE DVYDICWATD GNLMTSASVD
160 170 180 190 200
NTVIIWDVSK GQKISIFNEH KSYVQGVTWD PLGQYIATLS CDRVLRIYNT
210 220 230 240 250
QKKRVAFNIS KMLSGQGPEG EARSFRMFHD DSMKSFFRRL SFTPDGSLLL
260 270 280 290 300
TPAGCMESGE NVTNTTYVFS RKHLKRPIAH LPCPGKATLA VRCCPVYFEL
310 320 330 340 350
RPVAETEKAS EEPSPELVNL PYRMVFAVAS EDSVLLYDTQ QSFPFGYVSN
360 370 380 390 400
IHYHTLSDIS WSSDGAFLAI SSTDGYCTFV TFEKGELGIP LKEKPVLSIR
410 420 430 440 450
TPDTAKKAKN QTHQGSSPGS RSVEGTPSNR TQDPSSPCTT PSPTTQSPAP
460 470 480 490 500
SAIKDSPSAI PAGKSPLPQP SEEKTLQPAG QNMKAPQPRR VTLNTLQTWG
510 520 530 540 550
KTAPRRINLT PLKTDTVPNP QPNSGTAPST EEVQPEAPGE PPEEPPELKR
560 570
PRLEEREGDA QNLAPDDSSK TV
Length:572
Mass (Da):63,132
Last modified:June 1, 2001 - v1
Checksum:i03C2BC263591FF72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011243 mRNA. Translation: BAB27490.1.
BC013532 mRNA. Translation: AAH13532.1.
CCDSiCCDS28344.1.
RefSeqiNP_082359.1. NM_028083.4.
XP_006522910.1. XM_006522847.3.
UniGeneiMm.274222.

Genome annotation databases

EnsembliENSMUST00000023666; ENSMUSP00000023666; ENSMUSG00000022945.
ENSMUST00000117099; ENSMUSP00000113684; ENSMUSG00000022945.
GeneIDi110749.
KEGGimmu:110749.
UCSCiuc008aac.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011243 mRNA. Translation: BAB27490.1.
BC013532 mRNA. Translation: AAH13532.1.
CCDSiCCDS28344.1.
RefSeqiNP_082359.1. NM_028083.4.
XP_006522910.1. XM_006522847.3.
UniGeneiMm.274222.

3D structure databases

ProteinModelPortaliQ9D0N7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi225873. 2 interactors.
IntActiQ9D0N7. 2 interactors.
MINTiMINT-4614962.
STRINGi10090.ENSMUSP00000023666.

PTM databases

iPTMnetiQ9D0N7.
PhosphoSitePlusiQ9D0N7.

Proteomic databases

EPDiQ9D0N7.
MaxQBiQ9D0N7.
PaxDbiQ9D0N7.
PeptideAtlasiQ9D0N7.
PRIDEiQ9D0N7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023666; ENSMUSP00000023666; ENSMUSG00000022945.
ENSMUST00000117099; ENSMUSP00000113684; ENSMUSG00000022945.
GeneIDi110749.
KEGGimmu:110749.
UCSCiuc008aac.1. mouse.

Organism-specific databases

CTDi8208.
MGIiMGI:1314881. Chaf1b.

Phylogenomic databases

eggNOGiKOG1009. Eukaryota.
ENOG410XPU4. LUCA.
GeneTreeiENSGT00550000074968.
HOGENOMiHOG000183312.
HOVERGENiHBG050780.
InParanoidiQ9D0N7.
KOiK10751.
OMAiQDGYCTL.
OrthoDBiEOG091G0FIG.
PhylomeDBiQ9D0N7.
TreeFamiTF313062.

Miscellaneous databases

PROiQ9D0N7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022945.
CleanExiMM_CHAF1B.
ExpressionAtlasiQ9D0N7. baseline and differential.
GenevisibleiQ9D0N7. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR029129. CAF1_p60_C.
IPR001632. Gprotein_B.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF15512. CAF-1_p60_C. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAF1B_MOUSE
AccessioniPrimary (citable) accession number: Q9D0N7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.