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Protein

Chromatin assembly factor 1 subunit B

Gene

Chaf1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, DNA damage, DNA repair, DNA replication, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Chromatin assembly factor 1 subunit B
Short name:
CAF-1 subunit B
Alternative name(s):
Chromatin assembly factor I p60 subunit
Short name:
CAF-I 60 kDa subunit
Short name:
CAF-I p60
Gene namesi
Name:Chaf1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1314881 Chaf1b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508971 – 572Chromatin assembly factor 1 subunit BAdd BLAST572

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei401PhosphothreonineBy similarity1
Modified residuei416PhosphoserineBy similarity1
Modified residuei426PhosphothreonineBy similarity1
Modified residuei436PhosphoserineCombined sources1
Modified residuei440PhosphothreonineBy similarity1
Modified residuei456PhosphoserineCombined sources1
Modified residuei465PhosphoserineBy similarity1
Modified residuei501N6-acetyllysineBy similarity1
Modified residuei502PhosphothreonineSequence analysis1
Modified residuei510PhosphothreonineSequence analysis1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9D0N7
MaxQBiQ9D0N7
PaxDbiQ9D0N7
PeptideAtlasiQ9D0N7
PRIDEiQ9D0N7

PTM databases

iPTMnetiQ9D0N7
PhosphoSitePlusiQ9D0N7

Expressioni

Gene expression databases

BgeeiENSMUSG00000022945
CleanExiMM_CHAF1B
ExpressionAtlasiQ9D0N7 baseline and differential
GenevisibleiQ9D0N7 MM

Interactioni

Subunit structurei

Subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi225873, 2 interactors
IntActiQ9D0N7, 2 interactors
MINTiQ9D0N7
STRINGi10090.ENSMUSP00000023666

Structurei

3D structure databases

ProteinModelPortaliQ9D0N7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati11 – 54WD 1Add BLAST44
Repeati64 – 103WD 2Add BLAST40
Repeati127 – 166WD 3Add BLAST40
Repeati169 – 208WD 4Add BLAST40
Repeati228 – 279WD 5Add BLAST52
Repeati301 – 347WD 6Add BLAST47
Repeati351 – 392WD 7Add BLAST42

Sequence similaritiesi

Belongs to the WD repeat HIR1 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1009 Eukaryota
ENOG410XPU4 LUCA
GeneTreeiENSGT00550000074968
HOGENOMiHOG000183312
HOVERGENiHBG050780
InParanoidiQ9D0N7
KOiK10751
OMAiVYDICWT
OrthoDBiEOG091G0FIG
PhylomeDBiQ9D0N7
TreeFamiTF313062

Family and domain databases

Gene3Di2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR029129 CAF1_p60_C
IPR001632 Gprotein_B
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF15512 CAF-1_p60_C, 1 hit
PF00400 WD40, 4 hits
PRINTSiPR00319 GPROTEINB
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 2 hits
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 3 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Q9D0N7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVITCEIAW HNKEPVYSLD FQHGATWKIH RLASAGVDTA VRIWKLERGP
60 70 80 90 100
DGKAIVEFLS NLARHTKAVN VVRFSPTGEI LASGGDDAVI LLWKMNDSKE
110 120 130 140 150
PEQIAFQDEE EAQLNKENWT VVKTLRGHLE DVYDICWATD GNLMTSASVD
160 170 180 190 200
NTVIIWDVSK GQKISIFNEH KSYVQGVTWD PLGQYIATLS CDRVLRIYNT
210 220 230 240 250
QKKRVAFNIS KMLSGQGPEG EARSFRMFHD DSMKSFFRRL SFTPDGSLLL
260 270 280 290 300
TPAGCMESGE NVTNTTYVFS RKHLKRPIAH LPCPGKATLA VRCCPVYFEL
310 320 330 340 350
RPVAETEKAS EEPSPELVNL PYRMVFAVAS EDSVLLYDTQ QSFPFGYVSN
360 370 380 390 400
IHYHTLSDIS WSSDGAFLAI SSTDGYCTFV TFEKGELGIP LKEKPVLSIR
410 420 430 440 450
TPDTAKKAKN QTHQGSSPGS RSVEGTPSNR TQDPSSPCTT PSPTTQSPAP
460 470 480 490 500
SAIKDSPSAI PAGKSPLPQP SEEKTLQPAG QNMKAPQPRR VTLNTLQTWG
510 520 530 540 550
KTAPRRINLT PLKTDTVPNP QPNSGTAPST EEVQPEAPGE PPEEPPELKR
560 570
PRLEEREGDA QNLAPDDSSK TV
Length:572
Mass (Da):63,132
Last modified:June 1, 2001 - v1
Checksum:i03C2BC263591FF72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011243 mRNA Translation: BAB27490.1
BC013532 mRNA Translation: AAH13532.1
CCDSiCCDS28344.1
RefSeqiNP_082359.1, NM_028083.4
XP_006522910.1, XM_006522847.3
UniGeneiMm.274222

Genome annotation databases

EnsembliENSMUST00000023666; ENSMUSP00000023666; ENSMUSG00000022945
ENSMUST00000117099; ENSMUSP00000113684; ENSMUSG00000022945
GeneIDi110749
KEGGimmu:110749
UCSCiuc008aac.1 mouse

Similar proteinsi

Entry informationi

Entry nameiCAF1B_MOUSE
AccessioniPrimary (citable) accession number: Q9D0N7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health