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Protein

Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial

Gene

Oxct1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate (By similarity).By similarity

Catalytic activityi

Succinyl-CoA + a 3-oxo acid = succinate + a 3-oxoacyl-CoA.PROSITE-ProRule annotation

Pathwayi: succinyl-CoA degradation

This protein is involved in step 1 of the subpathway that synthesizes acetoacetyl-CoA from succinyl-CoA.
Proteins known to be involved in this subpathway in this organism are:
  1. Succinyl-CoA:3-ketoacid coenzyme A transferase 2A, mitochondrial (Oxct2a), Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial (Oxct1), Succinyl-CoA:3-ketoacid-coenzyme A transferase (Oxct1), Succinyl-CoA:3-ketoacid-coenzyme A transferase (Oxct1), Succinyl-CoA:3-ketoacid coenzyme A transferase 2B, mitochondrial (Oxct2b), Succinyl-CoA:3-ketoacid-coenzyme A transferase (Oxct2b), Succinyl-CoA:3-ketoacid-coenzyme A transferase (Oxct1)
This subpathway is part of the pathway succinyl-CoA degradation, which is itself part of Ketone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetoacetyl-CoA from succinyl-CoA, the pathway succinyl-CoA degradation and in Ketone metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3445-glutamyl coenzyme A thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • 3-oxoacid CoA-transferase activity Source: MGI
  • protein homodimerization activity Source: Ensembl

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

ReactomeiR-MMU-77108 Utilization of Ketone Bodies
UniPathwayiUPA00929; UER00894

Names & Taxonomyi

Protein namesi
Recommended name:
Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial (EC:2.8.3.5)
Alternative name(s):
3-oxoacid CoA-transferase 1
Somatic-type succinyl-CoA:3-oxoacid CoA-transferase
Short name:
SCOT-s
Gene namesi
Name:Oxct1
Synonyms:Oxct, Scot
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1914291 Oxct1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 39MitochondrionBy similarityAdd BLAST39
ChainiPRO_000000241440 – 520Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrialAdd BLAST481

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei170PhosphoserineBy similarity1
Modified residuei185N6-succinyllysineCombined sources1
Modified residuei418N6-succinyllysineCombined sources1
Modified residuei421N6-succinyllysineCombined sources1
Modified residuei455N6-succinyllysineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9D0K2
MaxQBiQ9D0K2
PaxDbiQ9D0K2
PeptideAtlasiQ9D0K2
PRIDEiQ9D0K2

2D gel databases

REPRODUCTION-2DPAGEiIPI00132653

PTM databases

iPTMnetiQ9D0K2
PhosphoSitePlusiQ9D0K2
SwissPalmiQ9D0K2

Expressioni

Gene expression databases

BgeeiENSMUSG00000022186
CleanExiMM_OXCT1
ExpressionAtlasiQ9D0K2 baseline and differential
GenevisibleiQ9D0K2 MM

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211895, 1 interactor
IntActiQ9D0K2, 6 interactors
MINTiQ9D0K2
STRINGi10090.ENSMUSP00000106318

Structurei

3D structure databases

ProteinModelPortaliQ9D0K2
SMRiQ9D0K2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-oxoacid CoA-transferase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3822 Eukaryota
COG1788 LUCA
COG2057 LUCA
GeneTreeiENSGT00390000009130
HOGENOMiHOG000221244
HOVERGENiHBG002310
InParanoidiQ9D0K2
KOiK01027
OMAiNPAYSGE
OrthoDBiEOG091G0D3X
PhylomeDBiQ9D0K2
TreeFamiTF313991

Family and domain databases

InterProiView protein in InterPro
IPR012792 3-oxoacid_CoA-transf_A
IPR012791 3-oxoacid_CoA-transf_B
IPR014388 3-oxoacid_CoA-transferase
IPR004165 CoA_trans_fam_I
IPR004164 CoA_transf_AS
IPR004163 CoA_transf_BS
IPR037171 NagB/RpiA_transferase-like
PANTHERiPTHR13707 PTHR13707, 1 hit
PfamiView protein in Pfam
PF01144 CoA_trans, 2 hits
PIRSFiPIRSF000858 SCOT-t, 1 hit
SMARTiView protein in SMART
SM00882 CoA_trans, 2 hits
SUPFAMiSSF100950 SSF100950, 2 hits
TIGRFAMsiTIGR02429 pcaI_scoA_fam, 1 hit
TIGR02428 pcaJ_scoB_fam, 1 hit
PROSITEiView protein in PROSITE
PS01273 COA_TRANSF_1, 1 hit
PS01274 COA_TRANSF_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D0K2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALKLLSSG LRLGASARSS RGALHKGCVC YFSVSTRHHT KFYTDPVEAV
60 70 80 90 100
KDIPNGATLL VGGFGLCGIP ENLIGALLKT GVKDLTAVSN NAGVDNFGLG
110 120 130 140 150
LLLRSKQIKR MISSYVGENA EFERQFLSGE LEVELTPQGT LAERIRAGGA
160 170 180 190 200
GVPAFYTSTG YGTLVQEGGS PIKYNKDGSV AIASKPREVR EFNGQHFILE
210 220 230 240 250
EAITGDFALV KAWKADRAGN VIFRKSARNF NLPMCKAAGT TVVEVEEIVD
260 270 280 290 300
IGSFAPEDIH IPKIYVHRLI KGEKYEKRIE RLSLRKEGDG KGKSGKPGGD
310 320 330 340 350
VRERIIKRAA LEFEDGMYAN LGIGIPLLAS NFISPNMTVH LQSENGVLGL
360 370 380 390 400
GPYPLKDEAD ADLINAGKET VTVLPGASFF SSDESFAMIR GGHVNLTMLG
410 420 430 440 450
AMQVSKYGDL ANWMIPGKMV KGMGGAMDLV SSSKTKVVVT MEHSAKGNAH
460 470 480 490 500
KIMEKCTLPL TGKQCVNRII TEKGVFDVDK KNGLTLIELW EGLTVDDIKK
510 520
STGCDFAVSP NLMPMQQIST
Length:520
Mass (Da):55,989
Last modified:June 1, 2001 - v1
Checksum:i6C8CB60C756FA6A9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti478V → G in BAB27562 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB085609 mRNA Translation: BAC05524.1
AK009027 mRNA Translation: BAB26035.1
AK011354 mRNA Translation: BAB27562.1
AK151662 mRNA Translation: BAE30590.1
AK167681 mRNA Translation: BAE39730.1
BC003422 mRNA Translation: AAH03422.1
CCDSiCCDS27361.1
PIRiPD0443
RefSeqiNP_077150.1, NM_024188.6
UniGeneiMm.13445

Genome annotation databases

EnsembliENSMUST00000110690; ENSMUSP00000106318; ENSMUSG00000022186
GeneIDi67041
KEGGimmu:67041
UCSCiuc007vck.2 mouse

Similar proteinsi

Entry informationi

Entry nameiSCOT1_MOUSE
AccessioniPrimary (citable) accession number: Q9D0K2
Secondary accession number(s): Q3TIW6, Q9CV92
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health