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Protein

Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial

Gene

Oxct1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate (By similarity).By similarity

Catalytic activityi

Succinyl-CoA + a 3-oxo acid = succinate + a 3-oxoacyl-CoA.PROSITE-ProRule annotation

Pathwayi: succinyl-CoA degradation

This protein is involved in step 1 of the subpathway that synthesizes acetoacetyl-CoA from succinyl-CoA.
Proteins known to be involved in this subpathway in this organism are:
  1. Succinyl-CoA:3-ketoacid coenzyme A transferase 2A, mitochondrial (Oxct2a), Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial (Oxct1), Succinyl-CoA:3-ketoacid-coenzyme A transferase (Oxct1), Succinyl-CoA:3-ketoacid-coenzyme A transferase (Oxct1), Succinyl-CoA:3-ketoacid coenzyme A transferase 2B, mitochondrial (Oxct2b), Succinyl-CoA:3-ketoacid-coenzyme A transferase (Oxct2b), Succinyl-CoA:3-ketoacid-coenzyme A transferase (Oxct1)
This subpathway is part of the pathway succinyl-CoA degradation, which is itself part of Ketone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetoacetyl-CoA from succinyl-CoA, the pathway succinyl-CoA degradation and in Ketone metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei344 – 34415-glutamyl coenzyme A thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  • 3-oxoacid CoA-transferase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiR-MMU-77108. Utilization of Ketone Bodies.
UniPathwayiUPA00929; UER00894.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial (EC:2.8.3.5)
Alternative name(s):
3-oxoacid CoA-transferase 1
Somatic-type succinyl-CoA:3-oxoacid CoA-transferase
Short name:
SCOT-s
Gene namesi
Name:Oxct1
Synonyms:Oxct, Scot
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1914291. Oxct1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3939MitochondrionBy similarityAdd
BLAST
Chaini40 – 520481Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrialPRO_0000002414Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei170 – 1701PhosphoserineBy similarity
Modified residuei185 – 1851N6-succinyllysineCombined sources
Modified residuei418 – 4181N6-succinyllysineCombined sources
Modified residuei421 – 4211N6-succinyllysineCombined sources
Modified residuei455 – 4551N6-succinyllysineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9D0K2.
MaxQBiQ9D0K2.
PaxDbiQ9D0K2.
PeptideAtlasiQ9D0K2.
PRIDEiQ9D0K2.

2D gel databases

REPRODUCTION-2DPAGEIPI00132653.

PTM databases

iPTMnetiQ9D0K2.
PhosphoSiteiQ9D0K2.
SwissPalmiQ9D0K2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022186.
CleanExiMM_OXCT1.
ExpressionAtlasiQ9D0K2. baseline and differential.
GenevisibleiQ9D0K2. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ9D0K2. 6 interactions.
MINTiMINT-1596659.
STRINGi10090.ENSMUSP00000106318.

Structurei

3D structure databases

ProteinModelPortaliQ9D0K2.
SMRiQ9D0K2. Positions 40-519.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-oxoacid CoA-transferase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3822. Eukaryota.
COG1788. LUCA.
COG2057. LUCA.
GeneTreeiENSGT00390000009130.
HOGENOMiHOG000221244.
HOVERGENiHBG002310.
InParanoidiQ9D0K2.
KOiK01027.
OMAiNIQFRHA.
OrthoDBiEOG091G0D3X.
PhylomeDBiQ9D0K2.
TreeFamiTF313991.

Family and domain databases

InterProiIPR012792. 3-oxoacid_CoA-transf_A.
IPR012791. 3-oxoacid_CoA-transf_B.
IPR014388. 3-oxoacid_CoA-transferase.
IPR004165. CoA_trans_fam_I.
IPR004164. CoA_transf_AS.
IPR004163. CoA_transf_BS.
[Graphical view]
PANTHERiPTHR13707. PTHR13707. 1 hit.
PfamiPF01144. CoA_trans. 2 hits.
[Graphical view]
PIRSFiPIRSF000858. SCOT-t. 1 hit.
SMARTiSM00882. CoA_trans. 2 hits.
[Graphical view]
TIGRFAMsiTIGR02429. pcaI_scoA_fam. 1 hit.
TIGR02428. pcaJ_scoB_fam. 1 hit.
PROSITEiPS01273. COA_TRANSF_1. 1 hit.
PS01274. COA_TRANSF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D0K2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALKLLSSG LRLGASARSS RGALHKGCVC YFSVSTRHHT KFYTDPVEAV
60 70 80 90 100
KDIPNGATLL VGGFGLCGIP ENLIGALLKT GVKDLTAVSN NAGVDNFGLG
110 120 130 140 150
LLLRSKQIKR MISSYVGENA EFERQFLSGE LEVELTPQGT LAERIRAGGA
160 170 180 190 200
GVPAFYTSTG YGTLVQEGGS PIKYNKDGSV AIASKPREVR EFNGQHFILE
210 220 230 240 250
EAITGDFALV KAWKADRAGN VIFRKSARNF NLPMCKAAGT TVVEVEEIVD
260 270 280 290 300
IGSFAPEDIH IPKIYVHRLI KGEKYEKRIE RLSLRKEGDG KGKSGKPGGD
310 320 330 340 350
VRERIIKRAA LEFEDGMYAN LGIGIPLLAS NFISPNMTVH LQSENGVLGL
360 370 380 390 400
GPYPLKDEAD ADLINAGKET VTVLPGASFF SSDESFAMIR GGHVNLTMLG
410 420 430 440 450
AMQVSKYGDL ANWMIPGKMV KGMGGAMDLV SSSKTKVVVT MEHSAKGNAH
460 470 480 490 500
KIMEKCTLPL TGKQCVNRII TEKGVFDVDK KNGLTLIELW EGLTVDDIKK
510 520
STGCDFAVSP NLMPMQQIST
Length:520
Mass (Da):55,989
Last modified:June 1, 2001 - v1
Checksum:i6C8CB60C756FA6A9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti478 – 4781V → G in BAB27562 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB085609 mRNA. Translation: BAC05524.1.
AK009027 mRNA. Translation: BAB26035.1.
AK011354 mRNA. Translation: BAB27562.1.
AK151662 mRNA. Translation: BAE30590.1.
AK167681 mRNA. Translation: BAE39730.1.
BC003422 mRNA. Translation: AAH03422.1.
CCDSiCCDS27361.1.
PIRiPD0443.
RefSeqiNP_077150.1. NM_024188.6.
UniGeneiMm.13445.

Genome annotation databases

EnsembliENSMUST00000110690; ENSMUSP00000106318; ENSMUSG00000022186.
GeneIDi67041.
KEGGimmu:67041.
UCSCiuc007vck.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB085609 mRNA. Translation: BAC05524.1.
AK009027 mRNA. Translation: BAB26035.1.
AK011354 mRNA. Translation: BAB27562.1.
AK151662 mRNA. Translation: BAE30590.1.
AK167681 mRNA. Translation: BAE39730.1.
BC003422 mRNA. Translation: AAH03422.1.
CCDSiCCDS27361.1.
PIRiPD0443.
RefSeqiNP_077150.1. NM_024188.6.
UniGeneiMm.13445.

3D structure databases

ProteinModelPortaliQ9D0K2.
SMRiQ9D0K2. Positions 40-519.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9D0K2. 6 interactions.
MINTiMINT-1596659.
STRINGi10090.ENSMUSP00000106318.

PTM databases

iPTMnetiQ9D0K2.
PhosphoSiteiQ9D0K2.
SwissPalmiQ9D0K2.

2D gel databases

REPRODUCTION-2DPAGEIPI00132653.

Proteomic databases

EPDiQ9D0K2.
MaxQBiQ9D0K2.
PaxDbiQ9D0K2.
PeptideAtlasiQ9D0K2.
PRIDEiQ9D0K2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000110690; ENSMUSP00000106318; ENSMUSG00000022186.
GeneIDi67041.
KEGGimmu:67041.
UCSCiuc007vck.2. mouse.

Organism-specific databases

CTDi5019.
MGIiMGI:1914291. Oxct1.

Phylogenomic databases

eggNOGiKOG3822. Eukaryota.
COG1788. LUCA.
COG2057. LUCA.
GeneTreeiENSGT00390000009130.
HOGENOMiHOG000221244.
HOVERGENiHBG002310.
InParanoidiQ9D0K2.
KOiK01027.
OMAiNIQFRHA.
OrthoDBiEOG091G0D3X.
PhylomeDBiQ9D0K2.
TreeFamiTF313991.

Enzyme and pathway databases

UniPathwayiUPA00929; UER00894.
ReactomeiR-MMU-77108. Utilization of Ketone Bodies.

Miscellaneous databases

ChiTaRSiOxct1. mouse.
PROiQ9D0K2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022186.
CleanExiMM_OXCT1.
ExpressionAtlasiQ9D0K2. baseline and differential.
GenevisibleiQ9D0K2. MM.

Family and domain databases

InterProiIPR012792. 3-oxoacid_CoA-transf_A.
IPR012791. 3-oxoacid_CoA-transf_B.
IPR014388. 3-oxoacid_CoA-transferase.
IPR004165. CoA_trans_fam_I.
IPR004164. CoA_transf_AS.
IPR004163. CoA_transf_BS.
[Graphical view]
PANTHERiPTHR13707. PTHR13707. 1 hit.
PfamiPF01144. CoA_trans. 2 hits.
[Graphical view]
PIRSFiPIRSF000858. SCOT-t. 1 hit.
SMARTiSM00882. CoA_trans. 2 hits.
[Graphical view]
TIGRFAMsiTIGR02429. pcaI_scoA_fam. 1 hit.
TIGR02428. pcaJ_scoB_fam. 1 hit.
PROSITEiPS01273. COA_TRANSF_1. 1 hit.
PS01274. COA_TRANSF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSCOT1_MOUSE
AccessioniPrimary (citable) accession number: Q9D0K2
Secondary accession number(s): Q3TIW6, Q9CV92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.