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Protein

ADP-ribosylation factor-like protein 2

Gene

Arl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Regulates formation of new microtubules and centrosome integrity. Prevents the TBCD-induced microtubule destruction. Participates in association with TBCD, in the disassembly of the apical junction complexes. Antagonizes the effect of TBCD on epithelial cell detachment and tight and adherens junctions disassembly. Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. Component of a regulated secretory pathway involved in Ca2+-dependent release of acetylcholine. Required for normal progress through the cell cycle.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei68GTP; via amide nitrogen1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi23 – 30GTP1 Publication8
Nucleotide bindingi66 – 70GTP1 Publication5
Nucleotide bindingi125 – 128GTP1 Publication4

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-422356. Regulation of insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-like protein 2
Gene namesi
Name:Arl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1928393. Arl2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi30T → L: Reduces affinity to GTP and GDP. Inhibits interaction with PDE6D. 1 Publication1
Mutagenesisi70Q → L: Does not reduce affinity fo GTP and GDP. Enhances interaction with PDE6D. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00002074542 – 184ADP-ribosylation factor-like protein 2Add BLAST183

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1
Modified residuei45PhosphoserineCombined sources1
Cross-linki71Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Not N-myristoylated.

Keywords - PTMi

Isopeptide bond, Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9D0J4.
MaxQBiQ9D0J4.
PaxDbiQ9D0J4.
PeptideAtlasiQ9D0J4.
PRIDEiQ9D0J4.

PTM databases

iPTMnetiQ9D0J4.
PhosphoSitePlusiQ9D0J4.

Expressioni

Tissue specificityi

Expressed in brain, lung, cerebellum, liver, kidney, hippocampus, spleen, cortex and heart (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000024944.
CleanExiMM_ARL2.
GenevisibleiQ9D0J4. MM.

Interactioni

Subunit structurei

Found in a complex with ARL2, ARL2BP and SLC25A6. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. Interacts with ELMOD2. The GTP-bound form interacts with ARL2BP. Interacts with TBCD; the GDP-bound form interacts preferentially with TBCD. Interacts with UNC119 (By similarity). Found in a complex with ARL2, ARL2BP and SLC25A4. The GTP-bound form interacts with PDE6D.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PDE6DO439246EBI-1033319,EBI-712685From a different organism.
UNC119Q134322EBI-1033319,EBI-711260From a different organism.

Protein-protein interaction databases

BioGridi207906. 2 interactors.
DIPiDIP-36659N.
IntActiQ9D0J4. 6 interactors.
MINTiMINT-236357.
STRINGi10090.ENSMUSP00000025893.

Structurei

Secondary structure

1184
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 12Combined sources11
Beta strandi17 – 22Combined sources6
Helixi29 – 36Combined sources8
Beta strandi48 – 57Combined sources10
Beta strandi60 – 67Combined sources8
Helixi71 – 74Combined sources4
Helixi75 – 80Combined sources6
Beta strandi85 – 92Combined sources8
Helixi96 – 98Combined sources3
Helixi99 – 110Combined sources12
Helixi113 – 115Combined sources3
Beta strandi119 – 125Combined sources7
Helixi135 – 141Combined sources7
Helixi144 – 146Combined sources3
Beta strandi152 – 156Combined sources5
Turni159 – 161Combined sources3
Helixi165 – 177Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KSGX-ray2.30A1-184[»]
1KSHX-ray1.80A1-184[»]
1KSJX-ray2.60A1-184[»]
4GOKX-ray2.60A/B17-184[»]
ProteinModelPortaliQ9D0J4.
SMRiQ9D0J4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9D0J4.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0073. Eukaryota.
ENOG410XRG9. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiQ9D0J4.
KOiK07943.
OMAiFEQTDAL.
OrthoDBiEOG091G0O6V.
PhylomeDBiQ9D0J4.
TreeFamiTF105462.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D0J4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLTILKKM KQKERELRLL MLGLDNAGKT TILKKFNGED VDTISPTLGF
60 70 80 90 100
NIKTLEHRGF KLNIWDVGGQ KSLRSYWRNY FESTDGLIWV VDSADRQRMQ
110 120 130 140 150
DCQRELQSLL VEERLAGATL LIFANKQDLP GALSCNAIQE ALELDSIRSH
160 170 180
HWRIQGCSAV TGEDLLPGID WLLDDISSRV FTAD
Length:184
Mass (Da):20,864
Last modified:June 1, 2001 - v1
Checksum:i8B3741700BFED3F8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33L → S in AAD33908 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143680 mRNA. Translation: AAD33908.1.
AK011366 mRNA. Translation: BAB27572.1.
BC060259 mRNA. Translation: AAH60259.1.
CCDSiCCDS29496.1.
RefSeqiNP_062696.2. NM_019722.3.
UniGeneiMm.21071.

Genome annotation databases

EnsembliENSMUST00000025893; ENSMUSP00000025893; ENSMUSG00000024944.
GeneIDi56327.
KEGGimmu:56327.
UCSCiuc008gho.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143680 mRNA. Translation: AAD33908.1.
AK011366 mRNA. Translation: BAB27572.1.
BC060259 mRNA. Translation: AAH60259.1.
CCDSiCCDS29496.1.
RefSeqiNP_062696.2. NM_019722.3.
UniGeneiMm.21071.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KSGX-ray2.30A1-184[»]
1KSHX-ray1.80A1-184[»]
1KSJX-ray2.60A1-184[»]
4GOKX-ray2.60A/B17-184[»]
ProteinModelPortaliQ9D0J4.
SMRiQ9D0J4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207906. 2 interactors.
DIPiDIP-36659N.
IntActiQ9D0J4. 6 interactors.
MINTiMINT-236357.
STRINGi10090.ENSMUSP00000025893.

PTM databases

iPTMnetiQ9D0J4.
PhosphoSitePlusiQ9D0J4.

Proteomic databases

EPDiQ9D0J4.
MaxQBiQ9D0J4.
PaxDbiQ9D0J4.
PeptideAtlasiQ9D0J4.
PRIDEiQ9D0J4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025893; ENSMUSP00000025893; ENSMUSG00000024944.
GeneIDi56327.
KEGGimmu:56327.
UCSCiuc008gho.1. mouse.

Organism-specific databases

CTDi402.
MGIiMGI:1928393. Arl2.

Phylogenomic databases

eggNOGiKOG0073. Eukaryota.
ENOG410XRG9. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiQ9D0J4.
KOiK07943.
OMAiFEQTDAL.
OrthoDBiEOG091G0O6V.
PhylomeDBiQ9D0J4.
TreeFamiTF105462.

Enzyme and pathway databases

ReactomeiR-MMU-422356. Regulation of insulin secretion.

Miscellaneous databases

ChiTaRSiArl2. mouse.
EvolutionaryTraceiQ9D0J4.
PROiQ9D0J4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024944.
CleanExiMM_ARL2.
GenevisibleiQ9D0J4. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARL2_MOUSE
AccessioniPrimary (citable) accession number: Q9D0J4
Secondary accession number(s): Q9WUM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.