Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Arginine--tRNA ligase, cytoplasmic

Gene

Rars

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis (PubMed:12060739). Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.By similarity1 Publication

Catalytic activityi

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei211L-arginineBy similarity1
Binding sitei384L-arginineBy similarity1
Binding sitei388L-arginineBy similarity1
Binding sitei412L-arginineBy similarity1

GO - Molecular functioni

  • arginine binding Source: Ensembl
  • arginine-tRNA ligase activity Source: CAFA
  • ATP binding Source: UniProtKB-KW
  • cadherin binding Source: MGI
  • tRNA binding Source: Ensembl

GO - Biological processi

  • arginyl-tRNA aminoacylation Source: CAFA

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine--tRNA ligase, cytoplasmic (EC:6.1.1.191 Publication)
Alternative name(s):
Arginyl-tRNA synthetase
Short name:
ArgRS
Gene namesi
Name:Rars
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1914297. Rars.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001516591 – 660Arginine--tRNA ligase, cytoplasmicAdd BLAST660

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9D0I9.
MaxQBiQ9D0I9.
PaxDbiQ9D0I9.
PeptideAtlasiQ9D0I9.
PRIDEiQ9D0I9.

PTM databases

iPTMnetiQ9D0I9.
PhosphoSitePlusiQ9D0I9.
SwissPalmiQ9D0I9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000018848.
CleanExiMM_RARS.
GenevisibleiQ9D0I9. MM.

Interactioni

Subunit structurei

Interacts (via N-terminus) with AIMP1 (via N-terminus); this stimulates its catalytic activity. Interacts (via N-terminus) with LARS2 (via C-terminus). Monomer (By similarity). Part of a multisubunit complex that groups tRNA ligases for Arg (RARS), Asp (DARS), Gln (QARS), Ile (IARS), Leu (LARS), Lys (KARS), Met (MARS) the bifunctional ligase for Glu and Pro (EPRS) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18 (PubMed:12060739). Interacts with QARS. Part of a complex composed of RARS, QARS and AIMP1 (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi222643. 3 interactors.
IntActiQ9D0I9. 7 interactors.
MINTiMINT-1869825.
STRINGi10090.ENSMUSP00000018992.

Structurei

3D structure databases

ProteinModelPortaliQ9D0I9.
SMRiQ9D0I9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 72Could be involved in the assembly of the multisynthetase complexAdd BLAST72
Regioni200 – 202L-arginine bindingBy similarity3
Regioni529 – 543Interaction with tRNABy similarityAdd BLAST15

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi201 – 212"HIGH" regionAdd BLAST12

Domaini

The alpha-helical N-terminus (residues 1-72) mediates interaction with AIMP1 and thereby contributes to the assembly of the multisynthetase complex.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1195. Eukaryota.
COG0018. LUCA.
GeneTreeiENSGT00530000063407.
HOVERGENiHBG029238.
InParanoidiQ9D0I9.
KOiK01887.
OMAiNKPLHLG.
OrthoDBiEOG091G03A6.
PhylomeDBiQ9D0I9.
TreeFamiTF106111.

Family and domain databases

CDDicd00671. ArgRS_core. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00123. Arg_tRNA_synth. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR036695. Arg-tRNA-synth_N_sf.
IPR035684. ArgRS_core.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
PANTHERiPTHR11956. PTHR11956. 1 hit.
PfamiView protein in Pfam
PF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
PRINTSiPR01038. TRNASYNTHARG.
SMARTiView protein in SMART
SM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsiTIGR00456. argS. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9D0I9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGLVAQCSA RLLQQEREIK ALTAEIDRLK NCGCLEASPS LEQLREENLK
60 70 80 90 100
LKYRLNILRR SLQEERRKPT KNMININSRL QEVFGCAIRA AYPDLENPPL
110 120 130 140 150
IVTPSQQPKF GDYQCNSAMG ISQMLKAKEQ KVSPREIAEN ITKHLPNNKY
160 170 180 190 200
IDKVEIAGPG FINVHLRKDF VSEQLTSLLV NGVQLPVLGD KEKVIVDFSS
210 220 230 240 250
PNIAKEMHVG HLRSTIIGES MSRLFEFAGY DVLRLNHVGD WGTQFGMLIA
260 270 280 290 300
HLQDKFPDYL TVSPPIGDLQ AFYKESKKRF DADEEFKKRA YQCVVLLQSK
310 320 330 340 350
NPDIMKAWNL ICDVSREEFK KIYDALDITL IERGESFYQD RMKDIVKEFE
360 370 380 390 400
DKGFVQVDDG RKIVFVPGCS VPLTIVKSDG GYTYDTSDLA AIKQRLFEEK
410 420 430 440 450
ANKIIYVVDN GQAIHFQTIF AAAQMIGWYD PKVTLVTHVG FGVVLGEDKK
460 470 480 490 500
KFKTRSGETV RLMDLLEEGL KRSMDKLKEK ERDKVLTEEE LKAAQTSVAY
510 520 530 540 550
GCIKYADLSH NRLNDYIFSF DKMLDDRGNT AAYLLYAFTR IRSIARLANI
560 570 580 590 600
DEAMLQRAAR ETKIILDHEK EWKLGRCILR FPEILQKILD DLFLHTLCDY
610 620 630 640 650
IYELATTFTE FYDSCYCVEK DRQTGKVLKV NMWRMLLCEA VAAVMAKGFD
660
ILGIKPVQRM
Length:660
Mass (Da):75,674
Last modified:April 16, 2002 - v2
Checksum:i6E8EBCC590FAB81D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5V → M in BAE30955 (PubMed:16141072).Curated1
Sequence conflicti178L → H in BAB27583 (PubMed:16141072).Curated1
Sequence conflicti184Q → E in BAE40154 (PubMed:16141072).Curated1
Sequence conflicti210G → V in BAB27583 (PubMed:16141072).Curated1
Sequence conflicti252L → M in BAE38569 (PubMed:16141072).Curated1
Sequence conflicti281D → G in BAE40154 (PubMed:16141072).Curated1
Sequence conflicti288K → E in BAE40154 (PubMed:16141072).Curated1
Sequence conflicti418T → I in BAE40154 (PubMed:16141072).Curated1
Sequence conflicti474M → L in BAB27583 (PubMed:16141072).Curated1
Sequence conflicti591D → N in BAE38569 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011383 mRNA. Translation: BAB27583.1.
AK076160 mRNA. Translation: BAC36226.1.
AK149856 mRNA. Translation: BAE29127.1.
AK151966 mRNA. Translation: BAE30837.1.
AK152108 mRNA. Translation: BAE30955.1.
AK166097 mRNA. Translation: BAE38569.1.
AK168194 mRNA. Translation: BAE40154.1.
AL596084 Genomic DNA. Translation: CAI24388.1.
CH466658 Genomic DNA. Translation: EDL16275.1.
BC020132 mRNA. Translation: AAH20132.1.
CCDSiCCDS24544.1.
RefSeqiNP_080212.2. NM_025936.3.
UniGeneiMm.284906.

Genome annotation databases

EnsembliENSMUST00000018992; ENSMUSP00000018992; ENSMUSG00000018848.
GeneIDi104458.
KEGGimmu:104458.
UCSCiuc007ilh.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiSYRC_MOUSE
AccessioniPrimary (citable) accession number: Q9D0I9
Secondary accession number(s): Q3THP2
, Q3TM73, Q3U8R2, Q3U930, Q5SXA8, Q8VDW1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: April 16, 2002
Last modified: October 25, 2017
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families