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Protein

Arginine--tRNA ligase, cytoplasmic

Gene

Rars

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis (PubMed:12060739). Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.By similarity1 Publication

Catalytic activityi

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei211L-arginineBy similarity1
Binding sitei384L-arginineBy similarity1
Binding sitei388L-arginineBy similarity1
Binding sitei412L-arginineBy similarity1

GO - Molecular functioni

  • arginine binding Source: MGI
  • arginine-tRNA ligase activity Source: CAFA
  • ATP binding Source: MGI
  • cadherin binding Source: MGI
  • tRNA binding Source: MGI

GO - Biological processi

  • arginyl-tRNA aminoacylation Source: CAFA

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine--tRNA ligase, cytoplasmic (EC:6.1.1.191 Publication)
Alternative name(s):
Arginyl-tRNA synthetase
Short name:
ArgRS
Gene namesi
Name:Rars
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1914297 Rars

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001516591 – 660Arginine--tRNA ligase, cytoplasmicAdd BLAST660

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9D0I9
MaxQBiQ9D0I9
PaxDbiQ9D0I9
PeptideAtlasiQ9D0I9
PRIDEiQ9D0I9

PTM databases

iPTMnetiQ9D0I9
PhosphoSitePlusiQ9D0I9
SwissPalmiQ9D0I9

Expressioni

Gene expression databases

BgeeiENSMUSG00000018848
CleanExiMM_RARS
GenevisibleiQ9D0I9 MM

Interactioni

Subunit structurei

Interacts (via N-terminus) with AIMP1 (via N-terminus); this stimulates its catalytic activity. Interacts (via N-terminus) with LARS2 (via C-terminus). Monomer (By similarity). Part of a multisubunit complex that groups tRNA ligases for Arg (RARS), Asp (DARS), Gln (QARS), Ile (IARS), Leu (LARS), Lys (KARS), Met (MARS) the bifunctional ligase for Glu and Pro (EPRS) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18 (PubMed:12060739). Interacts with QARS. Part of a complex composed of RARS, QARS and AIMP1 (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi222643, 2 interactors
IntActiQ9D0I9, 7 interactors
MINTiQ9D0I9
STRINGi10090.ENSMUSP00000018992

Structurei

3D structure databases

ProteinModelPortaliQ9D0I9
SMRiQ9D0I9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 72Could be involved in the assembly of the multisynthetase complexAdd BLAST72
Regioni200 – 202L-arginine bindingBy similarity3
Regioni529 – 543Interaction with tRNABy similarityAdd BLAST15

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi201 – 212"HIGH" regionAdd BLAST12

Domaini

The alpha-helical N-terminus (residues 1-72) mediates interaction with AIMP1 and thereby contributes to the assembly of the multisynthetase complex.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1195 Eukaryota
COG0018 LUCA
GeneTreeiENSGT00530000063407
HOVERGENiHBG029238
InParanoidiQ9D0I9
KOiK01887
OMAiNKPLHLG
OrthoDBiEOG091G03A6
PhylomeDBiQ9D0I9
TreeFamiTF106111

Family and domain databases

CDDicd00671 ArgRS_core, 1 hit
Gene3Di3.30.1360.70, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00123 Arg_tRNA_synth, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR001278 Arg-tRNA-ligase
IPR005148 Arg-tRNA-synth_N
IPR036695 Arg-tRNA-synth_N_sf
IPR035684 ArgRS_core
IPR008909 DALR_anticod-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
PANTHERiPTHR11956 PTHR11956, 1 hit
PfamiView protein in Pfam
PF03485 Arg_tRNA_synt_N, 1 hit
PF05746 DALR_1, 1 hit
PF00750 tRNA-synt_1d, 1 hit
PRINTSiPR01038 TRNASYNTHARG
SMARTiView protein in SMART
SM01016 Arg_tRNA_synt_N, 1 hit
SM00836 DALR_1, 1 hit
SUPFAMiSSF47323 SSF47323, 1 hit
SSF55190 SSF55190, 1 hit
TIGRFAMsiTIGR00456 argS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q9D0I9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGLVAQCSA RLLQQEREIK ALTAEIDRLK NCGCLEASPS LEQLREENLK
60 70 80 90 100
LKYRLNILRR SLQEERRKPT KNMININSRL QEVFGCAIRA AYPDLENPPL
110 120 130 140 150
IVTPSQQPKF GDYQCNSAMG ISQMLKAKEQ KVSPREIAEN ITKHLPNNKY
160 170 180 190 200
IDKVEIAGPG FINVHLRKDF VSEQLTSLLV NGVQLPVLGD KEKVIVDFSS
210 220 230 240 250
PNIAKEMHVG HLRSTIIGES MSRLFEFAGY DVLRLNHVGD WGTQFGMLIA
260 270 280 290 300
HLQDKFPDYL TVSPPIGDLQ AFYKESKKRF DADEEFKKRA YQCVVLLQSK
310 320 330 340 350
NPDIMKAWNL ICDVSREEFK KIYDALDITL IERGESFYQD RMKDIVKEFE
360 370 380 390 400
DKGFVQVDDG RKIVFVPGCS VPLTIVKSDG GYTYDTSDLA AIKQRLFEEK
410 420 430 440 450
ANKIIYVVDN GQAIHFQTIF AAAQMIGWYD PKVTLVTHVG FGVVLGEDKK
460 470 480 490 500
KFKTRSGETV RLMDLLEEGL KRSMDKLKEK ERDKVLTEEE LKAAQTSVAY
510 520 530 540 550
GCIKYADLSH NRLNDYIFSF DKMLDDRGNT AAYLLYAFTR IRSIARLANI
560 570 580 590 600
DEAMLQRAAR ETKIILDHEK EWKLGRCILR FPEILQKILD DLFLHTLCDY
610 620 630 640 650
IYELATTFTE FYDSCYCVEK DRQTGKVLKV NMWRMLLCEA VAAVMAKGFD
660
ILGIKPVQRM
Length:660
Mass (Da):75,674
Last modified:April 16, 2002 - v2
Checksum:i6E8EBCC590FAB81D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5V → M in BAE30955 (PubMed:16141072).Curated1
Sequence conflicti178L → H in BAB27583 (PubMed:16141072).Curated1
Sequence conflicti184Q → E in BAE40154 (PubMed:16141072).Curated1
Sequence conflicti210G → V in BAB27583 (PubMed:16141072).Curated1
Sequence conflicti252L → M in BAE38569 (PubMed:16141072).Curated1
Sequence conflicti281D → G in BAE40154 (PubMed:16141072).Curated1
Sequence conflicti288K → E in BAE40154 (PubMed:16141072).Curated1
Sequence conflicti418T → I in BAE40154 (PubMed:16141072).Curated1
Sequence conflicti474M → L in BAB27583 (PubMed:16141072).Curated1
Sequence conflicti591D → N in BAE38569 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011383 mRNA Translation: BAB27583.1
AK076160 mRNA Translation: BAC36226.1
AK149856 mRNA Translation: BAE29127.1
AK151966 mRNA Translation: BAE30837.1
AK152108 mRNA Translation: BAE30955.1
AK166097 mRNA Translation: BAE38569.1
AK168194 mRNA Translation: BAE40154.1
AL596084 Genomic DNA Translation: CAI24388.1
CH466658 Genomic DNA Translation: EDL16275.1
BC020132 mRNA Translation: AAH20132.1
CCDSiCCDS24544.1
RefSeqiNP_080212.2, NM_025936.3
UniGeneiMm.284906

Genome annotation databases

EnsembliENSMUST00000018992; ENSMUSP00000018992; ENSMUSG00000018848
GeneIDi104458
KEGGimmu:104458
UCSCiuc007ilh.2 mouse

Similar proteinsi

Entry informationi

Entry nameiSYRC_MOUSE
AccessioniPrimary (citable) accession number: Q9D0I9
Secondary accession number(s): Q3THP2
, Q3TM73, Q3U8R2, Q3U930, Q5SXA8, Q8VDW1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: April 16, 2002
Last modified: June 20, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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