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Q9D0F3 (LMAN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein ERGIC-53
Alternative name(s):
ER-Golgi intermediate compartment 53 kDa protein
Lectin mannose-binding 1
p58
Gene names
Name:Lman1
Synonyms:Ergic53
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length517 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins By similarity.

Subunit structure

Exists both as a covalent disulfide-linked homohexamer, and a complex of three disulfide-linked dimers non-covalently kept together. Interacts with MCFD2 By similarity.

Subcellular location

Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein By similarity. Golgi apparatus membrane; Single-pass membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass membrane protein By similarity.

Domain

The FF ER export motif at the C-terminus is not sufficient to support endoplasmic reticulum exit, and needs assistance of Gln-508 for proper recognition of COPII coat components By similarity.

Sequence similarities

Contains 1 L-type lectin-like domain.

Ontologies

Keywords
   Biological processER-Golgi transport
Protein transport
Transport
   Cellular componentEndoplasmic reticulum
Golgi apparatus
Membrane
   DomainSignal
Transmembrane
Transmembrane helix
   LigandLectin
Metal-binding
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processER to Golgi vesicle-mediated transport

Traceable author statement PubMed 11850423. Source: MGI

Golgi organization

Inferred from electronic annotation. Source: Ensembl

endoplasmic reticulum organization

Inferred from mutant phenotype PubMed 21795745. Source: MGI

positive regulation of organelle organization

Inferred from electronic annotation. Source: Ensembl

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentER to Golgi transport vesicle

Inferred from direct assay PubMed 19966784. Source: MGI

Golgi apparatus

Inferred from direct assay PubMed 10787428PubMed 11784862. Source: MGI

Golgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

endoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

endoplasmic reticulum-Golgi intermediate compartment

Inferred from direct assay PubMed 14645249PubMed 14728599PubMed 15452145PubMed 21187406. Source: MGI

endoplasmic reticulum-Golgi intermediate compartment membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

sarcomere

Inferred from direct assay PubMed 14728599. Source: MGI

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 Potential
Chain31 – 517487Protein ERGIC-53
PRO_0000017661

Regions

Topological domain31 – 484454Lumenal Potential
Transmembrane485 – 50521Helical; Potential
Topological domain506 – 51712Cytoplasmic Potential
Domain52 – 275224L-type lectin-like
Region259 – 2613Carbohydrate binding By similarity
Motif516 – 5172ER export motif By similarity

Sites

Metal binding1601Calcium By similarity
Metal binding1621Calcium; via carbonyl oxygen By similarity
Metal binding1641Calcium By similarity
Metal binding1891Calcium By similarity
Binding site961Carbohydrate By similarity
Binding site1291Carbohydrate By similarity
Binding site1641Carbohydrate By similarity
Binding site1861Carbohydrate By similarity
Site5081Required for ER export By similarity

Amino acid modifications

Disulfide bond198 ↔ 238 By similarity
Disulfide bond473Interchain By similarity
Disulfide bond482Interchain By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9D0F3 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: BA9FCFDEBCC5656D

FASTA51757,789
        10         20         30         40         50         60 
MAVSRRRVPQ AGARSFFCAL LLSFSQFTGS DGTGGDAAAP GAAGTQAELP HRRFEYKYSF 

        70         80         90        100        110        120 
KGPHLVQSDG TVPFWAHAGN AIPSADQIRI APSLKSQRGS VWTKAKAAFE NWEVEVTFRV 

       130        140        150        160        170        180 
TGRGRIGADG LAIWYTENQG LDGPVFGSAD TWNGVGIFFD SFDNDGKKNN PAIVVIGNNG 

       190        200        210        220        230        240 
QINYDHQNDG ATQALASCQR DFRNKPYPVR AKITYYQKTL TVMINNGFTP DKNDYEFCAK 

       250        260        270        280        290        300 
VENMVIPTQG HFGISAATGG LADDHDVLSF LTFQLTEPGK EPPTAEKDIS EKEKEKYQEE 

       310        320        330        340        350        360 
FEHFQQELDK KKEEFQKGHP DLQGQPADDI FESIGDRELR QVFEGQNRIH LEIKQLNRQL 

       370        380        390        400        410        420 
DMILDEQRRY VSSLTEEISR RGAGTPGQPG QVSQQELDTV VKSQQEILRQ VNEVKNSMSE 

       430        440        450        460        470        480 
TVRLVSGIQH PGSAGVYETT QHFMDIKEHL HVVKRDIDSL AQRSMPSNEK PKCPDLPPFP 

       490        500        510 
SCLSTIHFVI FVVVQTVLFV GYIMYRTQQE AAAKKFF 

« Hide

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Embryo.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK011495 mRNA. Translation: BAB27655.1.
BC057165 mRNA. Translation: AAH57165.1.
RefSeqNP_001165533.1. NM_001172062.1.
NP_081676.1. NM_027400.3.
UniGeneMm.290857.
Mm.449042.

3D structure databases

ProteinModelPortalQ9D0F3.
SMRQ9D0F3. Positions 43-277.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9D0F3. 6 interactions.
MINTMINT-1858436.

PTM databases

PhosphoSiteQ9D0F3.

Proteomic databases

PaxDbQ9D0F3.
PRIDEQ9D0F3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000048260; ENSMUSP00000040140; ENSMUSG00000041891.
ENSMUST00000120461; ENSMUSP00000113326; ENSMUSG00000041891.
GeneID70361.
KEGGmmu:70361.
UCSCuc008ffn.2. mouse.

Organism-specific databases

CTD3998.
MGIMGI:1917611. Lman1.

Phylogenomic databases

eggNOGNOG292314.
GeneTreeENSGT00530000062977.
HOVERGENHBG052332.
InParanoidQ9D0F3.
KOK10080.
OMAGTVPFWA.
OrthoDBEOG7QC7VR.
PhylomeDBQ9D0F3.
TreeFamTF313311.

Gene expression databases

ArrayExpressQ9D0F3.
BgeeQ9D0F3.
CleanExMM_LMAN1.
GenevestigatorQ9D0F3.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR005052. Lectin_leg.
[Graphical view]
PANTHERPTHR12223. PTHR12223. 1 hit.
PfamPF03388. Lectin_leg-like. 1 hit.
[Graphical view]
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS51328. L_LECTIN_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio331450.
PROQ9D0F3.
SOURCESearch...

Entry information

Entry nameLMAN1_MOUSE
AccessionPrimary (citable) accession number: Q9D0F3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot