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Protein

Kinetochore protein NDC80 homolog

Gene

Ndc80

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2500257. Resolution of Sister Chromatid Cohesion.
R-MMU-5663220. RHO GTPases Activate Formins.
R-MMU-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein NDC80 homolog
Alternative name(s):
Kinetochore protein Hec1
Kinetochore-associated protein 2
Gene namesi
Name:Ndc80
Synonyms:Hec1, Kntc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1914302. Ndc80.

Subcellular locationi

  • Nucleus By similarity
  • Chromosomecentromerekinetochore By similarity

  • Note: Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 642642Kinetochore protein NDC80 homologPRO_0000249552Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei44 – 441PhosphoserineBy similarity
Modified residuei69 – 691PhosphoserineBy similarity
Modified residuei165 – 1651Phosphoserine; by NEK2By similarity
Modified residuei242 – 2421PhosphoserineCombined sources

Post-translational modificationi

Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9D0F1.
MaxQBiQ9D0F1.
PaxDbiQ9D0F1.
PeptideAtlasiQ9D0F1.
PRIDEiQ9D0F1.

PTM databases

iPTMnetiQ9D0F1.
PhosphoSiteiQ9D0F1.

Expressioni

Tissue specificityi

Expressed in spleen, testis and thymus.1 Publication

Gene expression databases

BgeeiENSMUSG00000024056.
GenevisibleiQ9D0F1. MM.

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of KNTC2/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of KNTC2/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the KNTC2/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. Interacts with NEK2 and ZWINT specifically during mitosis. Interacts with CENPH and MIS12. May interact with AURKB, PSMC2, PSMC5 and SMC1A. May interact with RB1 during G2 phase and mitosis (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211904. 51 interactions.
IntActiQ9D0F1. 55 interactions.
MINTiMINT-218440.
STRINGi10090.ENSMUSP00000024851.

Structurei

3D structure databases

ProteinModelPortaliQ9D0F1.
SMRiQ9D0F1. Positions 80-286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 445445Interaction with the N-terminus of CDCA1By similarityAdd
BLAST
Regioni1 – 250250Nuclear localizationBy similarityAdd
BLAST
Regioni128 – 251124Interaction with RB1By similarityAdd
BLAST
Regioni251 – 618368Interaction with NEK2 and ZWINTBy similarityAdd
BLAST
Regioni251 – 431181Interaction with SMC1ABy similarityAdd
BLAST
Regioni361 – 547187Interaction with PSMC2 and SMC1ABy similarityAdd
BLAST
Regioni446 – 642197Interaction with the C-terminus of CDCA1 and the SPBC24-SPBC25 subcomplexBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili240 – 403164Sequence analysisAdd
BLAST
Coiled coili458 – 639182Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the NDC80/HEC1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0995. Eukaryota.
COG5185. LUCA.
GeneTreeiENSGT00390000018386.
HOGENOMiHOG000012981.
HOVERGENiHBG081861.
InParanoidiQ9D0F1.
KOiK11547.
OMAiEKESICA.
OrthoDBiEOG091G046M.
PhylomeDBiQ9D0F1.
TreeFamiTF101177.

Family and domain databases

InterProiIPR005550. Kinetochore_Ndc80.
[Graphical view]
PANTHERiPTHR10643. PTHR10643. 1 hit.
PfamiPF03801. Ndc80_HEC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9D0F1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRSSVSTCG AGRLSMQELR TLDLNKPGLY TPQTKERSTF GKLSTHKPTS
60 70 80 90 100
ERKVSIFGKR TSGHGSRNSQ LGIFSSSEKI KDPRPLNDKA FIQQCIRQLY
110 120 130 140 150
EFLTENGYVY SVSMKSLQAP STKEFLKIFA FLYGFLCPSY ELPGTKCEEE
160 170 180 190 200
VPRIFKALGY PFTLSKSSMY TVGAPHTWPH IVAALVWLID CIKIDTAMKE
210 220 230 240 250
SSPLFDDGQL WGEETEDGIK HNKLFLEYTK KCYEKFMTGA DSFEEEDAEL
260 270 280 290 300
QAKLKDLYKV DASKLESLEA ENKALNEQIA RLEEEREREP NRLMSLKKLK
310 320 330 340 350
ASLQADVQNY KAYMSNLESH LAVLKQKSNS LDEEIGRVEQ ECETVKQENT
360 370 380 390 400
RLQSIVDNQK YSVADIERIN HEKNELQQTI NKLTKDLEAE QQQMWNEELK
410 420 430 440 450
YARGKEAIEA QLAEYHKLAR KLKLIPKGAE NSKGYDFEIK FNPEAGANCL
460 470 480 490 500
VKYRTQVYAP LKELLNESEE EINKALNKKR HLEDTLEQLN TMKTESKNTV
510 520 530 540 550
RMLKEEIQKL DDLHQQAVKE AEEKDKKSAS ELESLEKHKH LLESGVNDGL
560 570 580 590 600
SEAMDELDAV QREYQLTVKT TTEERRKVEN NLQRLLEMVA THVGSLEKHL
610 620 630 640
EEENAKADRE YEEFMSEDLL ENIREMAEKY KRNAAQLKAP DK
Length:642
Mass (Da):73,962
Last modified:June 1, 2001 - v1
Checksum:i2BFF61F09B6E2CF8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131R → S in BAE37296 (PubMed:16141072).Curated
Sequence conflicti274 – 2741A → E in AAK01425 (PubMed:11266451).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF326730 mRNA. Translation: AAK01425.1.
AK011497 mRNA. Translation: BAB27657.1.
AK136236 mRNA. Translation: BAE22889.1.
AK163317 mRNA. Translation: BAE37296.1.
AK169427 mRNA. Translation: BAE41169.1.
BC020131 mRNA. Translation: AAH20131.1.
CCDSiCCDS28959.1.
RefSeqiNP_075783.2. NM_023294.2.
UniGeneiMm.225956.

Genome annotation databases

EnsembliENSMUST00000024851; ENSMUSP00000024851; ENSMUSG00000024056.
GeneIDi67052.
KEGGimmu:67052.
UCSCiuc008dmk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF326730 mRNA. Translation: AAK01425.1.
AK011497 mRNA. Translation: BAB27657.1.
AK136236 mRNA. Translation: BAE22889.1.
AK163317 mRNA. Translation: BAE37296.1.
AK169427 mRNA. Translation: BAE41169.1.
BC020131 mRNA. Translation: AAH20131.1.
CCDSiCCDS28959.1.
RefSeqiNP_075783.2. NM_023294.2.
UniGeneiMm.225956.

3D structure databases

ProteinModelPortaliQ9D0F1.
SMRiQ9D0F1. Positions 80-286.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211904. 51 interactions.
IntActiQ9D0F1. 55 interactions.
MINTiMINT-218440.
STRINGi10090.ENSMUSP00000024851.

PTM databases

iPTMnetiQ9D0F1.
PhosphoSiteiQ9D0F1.

Proteomic databases

EPDiQ9D0F1.
MaxQBiQ9D0F1.
PaxDbiQ9D0F1.
PeptideAtlasiQ9D0F1.
PRIDEiQ9D0F1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024851; ENSMUSP00000024851; ENSMUSG00000024056.
GeneIDi67052.
KEGGimmu:67052.
UCSCiuc008dmk.2. mouse.

Organism-specific databases

CTDi10403.
MGIiMGI:1914302. Ndc80.

Phylogenomic databases

eggNOGiKOG0995. Eukaryota.
COG5185. LUCA.
GeneTreeiENSGT00390000018386.
HOGENOMiHOG000012981.
HOVERGENiHBG081861.
InParanoidiQ9D0F1.
KOiK11547.
OMAiEKESICA.
OrthoDBiEOG091G046M.
PhylomeDBiQ9D0F1.
TreeFamiTF101177.

Enzyme and pathway databases

ReactomeiR-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2500257. Resolution of Sister Chromatid Cohesion.
R-MMU-5663220. RHO GTPases Activate Formins.
R-MMU-68877. Mitotic Prometaphase.

Miscellaneous databases

PROiQ9D0F1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024056.
GenevisibleiQ9D0F1. MM.

Family and domain databases

InterProiIPR005550. Kinetochore_Ndc80.
[Graphical view]
PANTHERiPTHR10643. PTHR10643. 1 hit.
PfamiPF03801. Ndc80_HEC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDC80_MOUSE
AccessioniPrimary (citable) accession number: Q9D0F1
Secondary accession number(s): Q3TQT6, Q3UWM5, Q99P70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.