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Protein

General transcription factor IIE subunit 1

Gene

Gtf2e1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri129 – 15729C4-typeSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. sequence-specific DNA binding Source: InterPro

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription initiation from RNA polymerase II promoter Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_282911. RNA Polymerase II Pre-transcription Events.
REACT_283211. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_329615. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_342690. RNA Polymerase II Transcription Initiation.
REACT_349121. RNA Polymerase II Promoter Escape.

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIE subunit 1
Alternative name(s):
General transcription factor IIE 56 kDa subunit
Transcription initiation factor IIE subunit alpha
Short name:
TFIIE-alpha
Gene namesi
Name:Gtf2e1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1921447. Gtf2e1.

Subcellular locationi

GO - Cellular componenti

  1. intermediate filament cytoskeleton Source: MGI
  2. nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 440439General transcription factor IIE subunit 1PRO_0000211223Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei67 – 671N6-acetyllysineBy similarity
Modified residuei268 – 2681PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9D0D5.
PaxDbiQ9D0D5.
PRIDEiQ9D0D5.

PTM databases

PhosphoSiteiQ9D0D5.

Expressioni

Gene expression databases

BgeeiQ9D0D5.
GenevestigatoriQ9D0D5.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains. Interacts with TAF6/TAFII80 (By similarity). Interacts with ATF7IP (By similarity).By similarity

Protein-protein interaction databases

BioGridi216568. 12 interactions.
IntActiQ9D0D5. 1 interaction.
MINTiMINT-4136705.

Structurei

3D structure databases

ProteinModelPortaliQ9D0D5.
SMRiQ9D0D5. Positions 113-174, 395-440.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 10491HTH TFE/IIEalpha-typePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi380 – 39415Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Belongs to the TFIIE alpha subunit family.Curated
Contains 1 HTH TFE/IIEalpha-type domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri129 – 15729C4-typeSequence AnalysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG1675.
GeneTreeiENSGT00390000016696.
HOGENOMiHOG000007666.
HOVERGENiHBG008614.
InParanoidiQ9D0D5.
KOiK03136.
OMAiQEDHRAS.
OrthoDBiEOG7NGQBK.
PhylomeDBiQ9D0D5.
TreeFamiTF313429.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR017919. TFIIE/TFIIEa_HTH.
IPR002853. TFIIE_asu.
IPR021600. TFIIE_asu_C.
IPR024550. TFIIEa/SarR/Rpc3_HTH_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR013137. Znf_TFIIB.
[Graphical view]
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
PF11521. TFIIE-A_C-term. 1 hit.
PF02002. TFIIE_alpha. 1 hit.
[Graphical view]
SMARTiSM00531. TFIIE. 1 hit.
[Graphical view]
PROSITEiPS51344. HTH_TFE_IIE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D0D5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADPDVLTEV PAALKRLAKY VIRGFYGIEH VLALDILIRN PCVKEEDMLE
60 70 80 90 100
LLKFDRKQLR SVLNNLKGDK FIKCRMRVET AADGKTTRHN YYFINYRTLV
110 120 130 140 150
NVVKYKLDHM RRRIETDERN STNRASFKCP VCCSTFTDLE ANQLFDPMTG
160 170 180 190 200
TFRCTFCHTE VEEDESAMPK KDARTLLARF NEQIEPIYAL LRETEDVNLA
210 220 230 240 250
YEILEPEPTE IPALKQSKDR AATAAGAAGL AGGHHREAWT NKGPSYEDLY
260 270 280 290 300
TQNVVINMDD QDDVHRPSLE GKAAKERPIW LRESTVQGAY SSEEMKEGGI
310 320 330 340 350
DVDTFQEREE ARAGPDDNEE VMRALLIHEK KTSSVTAGSV GAAAPVTAAN
360 370 380 390 400
GSDSESETSE SDDDSPPRPA AAAPPHHHRD EDEEDEEFEE VADDPIVMVA
410 420 430 440
GCPFSYSEVS QRPELVAQMT PEEKEAYIAM GQRMFEDLFE
Length:440
Mass (Da):49,593
Last modified:May 31, 2001 - v1
Checksum:i0587E4DFE6FA0442
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011543 mRNA. Translation: BAB27686.1.
AK031528 mRNA. Translation: BAC27436.1.
AK154283 mRNA. Translation: BAE32487.1.
BC056966 mRNA. Translation: AAH56966.1.
CCDSiCCDS28160.1.
RefSeqiNP_083088.1. NM_028812.3.
XP_006522722.1. XM_006522659.2.
UniGeneiMm.287795.

Genome annotation databases

EnsembliENSMUST00000023525; ENSMUSP00000023525; ENSMUSG00000022828.
GeneIDi74197.
KEGGimmu:74197.
UCSCiuc007zeb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011543 mRNA. Translation: BAB27686.1.
AK031528 mRNA. Translation: BAC27436.1.
AK154283 mRNA. Translation: BAE32487.1.
BC056966 mRNA. Translation: AAH56966.1.
CCDSiCCDS28160.1.
RefSeqiNP_083088.1. NM_028812.3.
XP_006522722.1. XM_006522659.2.
UniGeneiMm.287795.

3D structure databases

ProteinModelPortaliQ9D0D5.
SMRiQ9D0D5. Positions 113-174, 395-440.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216568. 12 interactions.
IntActiQ9D0D5. 1 interaction.
MINTiMINT-4136705.

PTM databases

PhosphoSiteiQ9D0D5.

Proteomic databases

MaxQBiQ9D0D5.
PaxDbiQ9D0D5.
PRIDEiQ9D0D5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023525; ENSMUSP00000023525; ENSMUSG00000022828.
GeneIDi74197.
KEGGimmu:74197.
UCSCiuc007zeb.1. mouse.

Organism-specific databases

CTDi2960.
MGIiMGI:1921447. Gtf2e1.

Phylogenomic databases

eggNOGiCOG1675.
GeneTreeiENSGT00390000016696.
HOGENOMiHOG000007666.
HOVERGENiHBG008614.
InParanoidiQ9D0D5.
KOiK03136.
OMAiQEDHRAS.
OrthoDBiEOG7NGQBK.
PhylomeDBiQ9D0D5.
TreeFamiTF313429.

Enzyme and pathway databases

ReactomeiREACT_282911. RNA Polymerase II Pre-transcription Events.
REACT_283211. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_329615. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_342690. RNA Polymerase II Transcription Initiation.
REACT_349121. RNA Polymerase II Promoter Escape.

Miscellaneous databases

NextBioi340074.
PROiQ9D0D5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9D0D5.
GenevestigatoriQ9D0D5.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR017919. TFIIE/TFIIEa_HTH.
IPR002853. TFIIE_asu.
IPR021600. TFIIE_asu_C.
IPR024550. TFIIEa/SarR/Rpc3_HTH_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR013137. Znf_TFIIB.
[Graphical view]
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
PF11521. TFIIE-A_C-term. 1 hit.
PF02002. TFIIE_alpha. 1 hit.
[Graphical view]
SMARTiSM00531. TFIIE. 1 hit.
[Graphical view]
PROSITEiPS51344. HTH_TFE_IIE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Embryo.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiT2EA_MOUSE
AccessioniPrimary (citable) accession number: Q9D0D5
Secondary accession number(s): Q544T0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 26, 2002
Last sequence update: May 31, 2001
Last modified: March 31, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.