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Q9D0D3

- PAPD1_MOUSE

UniProt

Q9D0D3 - PAPD1_MOUSE

Protein

Poly(A) RNA polymerase, mitochondrial

Gene

Mtpap

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA By similarity.By similarity

    Catalytic activityi

    ATP + RNA(n) = diphosphate + RNA(n+1).

    Cofactori

    Magnesium or manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi246 – 2461Magnesium or manganese; catalyticSequence Analysis
    Metal bindingi248 – 2481Magnesium or manganese; catalyticSequence Analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi107 – 1093ATPSequence Analysis
    Nucleotide bindingi244 – 2452ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. manganese ion binding Source: UniProtKB
    4. polynucleotide adenylyltransferase activity Source: UniProtKB
    5. RNA binding Source: UniProtKB-KW
    6. UTP binding Source: UniProtKB

    GO - Biological processi

    1. histone mRNA catabolic process Source: UniProtKB
    2. mRNA polyadenylation Source: UniProtKB
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    mRNA processing, Transcription

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Poly(A) RNA polymerase, mitochondrial (EC:2.7.7.19)
    Short name:
    PAP
    Alternative name(s):
    PAP-associated domain-containing protein 1
    Polynucleotide adenylyltransferase
    Gene namesi
    Name:Mtpap
    Synonyms:Papd1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:1914690. Mtpap.

    Subcellular locationi

    Cytoplasm By similarity. Mitochondrion By similarity

    GO - Cellular componenti

    1. Golgi apparatus Source: Ensembl
    2. mitochondrion Source: MGI
    3. nucleus Source: Ensembl
    4. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3737MitochondrionSequence AnalysisAdd
    BLAST
    Chaini38 – 585548Poly(A) RNA polymerase, mitochondrialPRO_0000250690Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei90 – 901N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ9D0D3.
    PaxDbiQ9D0D3.
    PRIDEiQ9D0D3.

    PTM databases

    PhosphoSiteiQ9D0D3.

    Expressioni

    Gene expression databases

    BgeeiQ9D0D3.
    CleanExiMM_PAPD1.
    GenevestigatoriQ9D0D3.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000025077.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9D0D3.
    SMRiQ9D0D3. Positions 62-535.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini441 – 48646PAP-associatedAdd
    BLAST

    Sequence similaritiesi

    Belongs to the DNA polymerase type-B-like family.Curated
    Contains 1 PAP-associated domain.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG5260.
    GeneTreeiENSGT00550000074490.
    HOGENOMiHOG000115438.
    HOVERGENiHBG082104.
    InParanoidiQ9D0D3.
    KOiK18060.
    OMAiDAEDKCI.
    OrthoDBiEOG7353WD.
    PhylomeDBiQ9D0D3.
    TreeFamiTF354308.

    Family and domain databases

    InterProiIPR002058. PAP_assoc.
    [Graphical view]
    PfamiPF03828. PAP_assoc. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9D0D3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAARGVGLLT RLPVCSQRRN RIPRSISRLL SCPGTIAASI GSEEQSSVVA    50
    ETGIEDKTLQ KKFSEVQKER REQAQRTVLI HCPNNINEKK FLKYLSQHGP 100
    VNNHFFYESF GLFAVVEFCQ KDSIKSLQNG THTPTQSTEA AIPFKSRFLN 150
    LRLKNPSSQV SGQPFVQTTN QSPPSSKKLF ELLSYAESIE EQLNTLLKAF 200
    QLTEENIRLR HLTCSLIEDI AAAYFPSCVI RPFGSSVNTF GKLGCDLDMF 250
    LDLDETGKLD VHKNTGNFFM EFQVKNVPSE RIATQKILSV IGECLDNFGP 300
    GCVGVQKILN ARCPLVRFSH QGSGFQCDLT ANNSIALKSS ELLYIYGSLD 350
    SRVRALVFSV RCWARAHSLT SSIPGAWITN FSLTVMVIFF LQRRSPPILP 400
    TLDSLKSIAD AEDRCILEGN NCTFVQDVNK IQPSGNTETL ELLIKEFFEY 450
    FGNFAFNKNS INIRQGREQN KPDSSPLYIQ NPFETSLNIS KNVSQSQLQK 500
    FVELARDSAW ILEQEDKNQP FSSSRQPWGL AALLLPPGSG HTSLSRKKKK 550
    KPMSEKVKGL LASIKSNSPD SSTDTSGKRT ISTQA 585
    Length:585
    Mass (Da):65,229
    Last modified:June 1, 2001 - v1
    Checksum:i33DB6AC61A080B82
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti74 – 741A → S in BAE22572. (PubMed:15489334)Curated
    Sequence conflicti190 – 1901E → G in BAC36396. (PubMed:15489334)Curated
    Sequence conflicti249 – 2491M → T in BAE22572. (PubMed:15489334)Curated
    Sequence conflicti578 – 5781K → E in BAC36396. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK011546 mRNA. Translation: BAB27689.1.
    AK076565 mRNA. Translation: BAC36396.1.
    AK135537 mRNA. Translation: BAE22572.1.
    BC057643 mRNA. Translation: AAH57643.1.
    CCDSiCCDS37718.1.
    RefSeqiNP_080433.1. NM_026157.2.
    UniGeneiMm.49826.

    Genome annotation databases

    EnsembliENSMUST00000025077; ENSMUSP00000025077; ENSMUSG00000024234.
    GeneIDi67440.
    KEGGimmu:67440.
    UCSCiuc008dyi.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK011546 mRNA. Translation: BAB27689.1 .
    AK076565 mRNA. Translation: BAC36396.1 .
    AK135537 mRNA. Translation: BAE22572.1 .
    BC057643 mRNA. Translation: AAH57643.1 .
    CCDSi CCDS37718.1.
    RefSeqi NP_080433.1. NM_026157.2.
    UniGenei Mm.49826.

    3D structure databases

    ProteinModelPortali Q9D0D3.
    SMRi Q9D0D3. Positions 62-535.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000025077.

    PTM databases

    PhosphoSitei Q9D0D3.

    Proteomic databases

    MaxQBi Q9D0D3.
    PaxDbi Q9D0D3.
    PRIDEi Q9D0D3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025077 ; ENSMUSP00000025077 ; ENSMUSG00000024234 .
    GeneIDi 67440.
    KEGGi mmu:67440.
    UCSCi uc008dyi.1. mouse.

    Organism-specific databases

    CTDi 55149.
    MGIi MGI:1914690. Mtpap.

    Phylogenomic databases

    eggNOGi COG5260.
    GeneTreei ENSGT00550000074490.
    HOGENOMi HOG000115438.
    HOVERGENi HBG082104.
    InParanoidi Q9D0D3.
    KOi K18060.
    OMAi DAEDKCI.
    OrthoDBi EOG7353WD.
    PhylomeDBi Q9D0D3.
    TreeFami TF354308.

    Miscellaneous databases

    NextBioi 324570.
    PROi Q9D0D3.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9D0D3.
    CleanExi MM_PAPD1.
    Genevestigatori Q9D0D3.

    Family and domain databases

    InterProi IPR002058. PAP_assoc.
    [Graphical view ]
    Pfami PF03828. PAP_assoc. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo, Muellerian duct and Testis.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiPAPD1_MOUSE
    AccessioniPrimary (citable) accession number: Q9D0D3
    Secondary accession number(s): Q3UXJ1, Q8C651
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2006
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3