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Protein

tRNA (guanine(37)-N1)-methyltransferase

Gene

Trmt5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in mitochondrial tRNA methylation (By similarity). Specifically methylates the N1 position of guanosine-37 in various tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.UniRule annotationBy similarity

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N1-methylguanine(37) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei282S-adenosyl-L-methionineUniRule annotation1
Binding sitei380S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine(37)-N1)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
Alternative name(s):
M1G-methyltransferaseUniRule annotation
tRNA [GM37] methyltransferaseUniRule annotation
tRNA methyltransferase 5 homologUniRule annotation
Gene namesi
Name:Trmt5
Synonyms:Kiaa1393, Trm5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1923607 Trmt5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002565151 – 501tRNA (guanine(37)-N1)-methyltransferaseAdd BLAST501

Proteomic databases

EPDiQ9D0C4
PaxDbiQ9D0C4
PeptideAtlasiQ9D0C4
PRIDEiQ9D0C4

PTM databases

iPTMnetiQ9D0C4
PhosphoSitePlusiQ9D0C4

Expressioni

Gene expression databases

BgeeiENSMUSG00000034442
ExpressionAtlasiQ9D0C4 baseline and differential
GenevisibleiQ9D0C4 MM

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

IntActiQ9D0C4, 1 interactor
MINTiQ9D0C4
STRINGi10090.ENSMUSP00000112121

Structurei

3D structure databases

ProteinModelPortaliQ9D0C4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni320 – 321S-adenosyl-L-methionine bindingUniRule annotation2
Regioni348 – 349S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2078 Eukaryota
COG2520 LUCA
GeneTreeiENSGT00390000008208
HOGENOMiHOG000195270
HOVERGENiHBG094093
InParanoidiQ9D0C4
KOiK15429
OMAiKHLIGQV
OrthoDBiEOG091G08O8
PhylomeDBiQ9D0C4
TreeFamiTF315073

Family and domain databases

HAMAPiMF_03152 TRM5, 1 hit
InterProiView protein in InterPro
IPR030382 MeTrfase_TRM5/TYW2
IPR029063 SAM-dependent_MTases
IPR025792 tRNA_Gua_MeTrfase_euk
PfamiView protein in Pfam
PF02475 Met_10, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51684 SAM_MT_TRM5_TYW2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D0C4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRIVWKLFGF SRRLLQVEWC HPSESILLFT LVPRLRKAPS VFLLGQRQGL
60 70 80 90 100
STMPEIEASV RDSELFSPPS DVRGMRELDR TAFKKTVSIP VLKARKEVVN
110 120 130 140 150
RLMRALRRVA LQRPGIKRVI EDPKDEDSRL IMLDPYRMLT ADSFDKAELG
160 170 180 190 200
VLKELDVSPQ LSQYNLELTY ENFKSEEILK AVLPEGQDVT SGFSRVGHIA
210 220 230 240 250
HLNLRDHQLP FKHLIGQVMV DKNPGITSAV NKTSNIDNTY RNFQMEVLCG
260 270 280 290 300
EENMLTKVRE NNYTYEFDFS KVYWNPRLST EHGRITELLN PGDVLFDVFA
310 320 330 340 350
GVGPFAIPAA RKNCTVFAND LNPESHKWLL HNCKLNKVDQ KVKVFNMDGK
360 370 380 390 400
DFIQGPVREE LMLRLGLSAE AKPSVHIVMN LPAKAIEFLS VFRSLLDGQP
410 420 430 440 450
CSTELLPTVH CYCFSKDSDP AKDVRQQAEA VLGVSLETSS SVHLVRNVAP
460 470 480 490 500
NKEMLCITFQ IPTATLYRNQ SLSLQNDQEP PLKRQKTGDP FSGEPQIASD

S
Length:501
Mass (Da):56,794
Last modified:June 1, 2001 - v1
Checksum:i55816182B08766C0
GO
Isoform 2 (identifier: Q9D0C4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     475-501: QNDQEPPLKRQKTGDPFSGEPQIASDS → REFFTMKCGVYYP

Show »
Length:487
Mass (Da):55,465
Checksum:i1165769DA919820F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti334K → Q in BAD90443 (Ref. 3) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021355475 – 501QNDQE…IASDS → REFFTMKCGVYYP in isoform 2. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011576 mRNA Translation: BAB27710.1
BC012521 mRNA Translation: AAH12521.1
AK220389 mRNA Translation: BAD90443.1
CCDSiCCDS36474.1 [Q9D0C4-1]
RefSeqiNP_083856.1, NM_029580.3 [Q9D0C4-1]
UniGeneiMm.311344

Genome annotation databases

EnsembliENSMUST00000116420; ENSMUSP00000112121; ENSMUSG00000034442 [Q9D0C4-1]
GeneIDi76357
KEGGimmu:76357
UCSCiuc007nwf.1 mouse [Q9D0C4-2]
uc007nwg.1 mouse [Q9D0C4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTRM5_MOUSE
AccessioniPrimary (citable) accession number: Q9D0C4
Secondary accession number(s): Q5DTY1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
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