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Protein

E3 ubiquitin-protein ligase RNF115

Gene

Rnf115

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates E2-dependent, 'Lys-48'- and/or 'Lys-63'-linked polyubiquitination of substrates and may play a role in diverse biological processes. Through their polyubiquitination, may play a role in the endosomal trafficking and degradation of membrane receptors including EGFR, FLT3, MET and CXCR4.1 Publication1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.2 Publications
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri229 – 270RING-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF115Curated (EC:2.3.2.272 Publications)
Alternative name(s):
RING finger protein 115Imported
RING-type E3 ubiquitin transferase RNF115Curated
Rabring 71 Publication
Zinc finger protein 364By similarity
Gene namesi
Name:Rnf115Imported
Synonyms:Zfp364Imported, Znf364By similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1915095. Rnf115.

Subcellular locationi

  • Cytoplasmcytosol 1 Publication

  • Note: The GTP-bound form of RAB7A recruits RNF115 from the cytosol onto late endosomes/lysosomes.1 Publication

GO - Cellular componenti

  • cytosol Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi22C → A: Loss of interaction with EGFR and FLT3. No effect on E3 ubiquitin protein ligase activity; when associated with A-25. 1 Publication1
Mutagenesisi25C → A: Loss of interaction with EGFR and FLT3. No effect on E3 ubiquitin protein ligase activity; when associated with A-22. 1 Publication1
Mutagenesisi229C → S: Loss of E3 ubiquitin protein ligase activity. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000563152 – 305E3 ubiquitin-protein ligase RNF115Add BLAST304

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Post-translational modificationi

RING-type zinc finger-dependent and E2-dependent autoubiquitination.1 Publication

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

MaxQBiQ9D0C1.
PaxDbiQ9D0C1.
PRIDEiQ9D0C1.

PTM databases

iPTMnetiQ9D0C1.
PhosphoSitePlusiQ9D0C1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028098.
ExpressionAtlasiQ9D0C1. baseline and differential.
GenevisibleiQ9D0C1. MM.

Interactioni

Subunit structurei

Interacts with RAB7A. Interacts with EGFR and FLT3.2 Publications

Protein-protein interaction databases

BioGridi212475. 7 interactors.
IntActiQ9D0C1. 2 interactors.
MINTiMINT-217430.
STRINGi10090.ENSMUSP00000029740.

Structurei

3D structure databases

ProteinModelPortaliQ9D0C1.
SMRiQ9D0C1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri229 – 270RING-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0800. Eukaryota.
ENOG41121N2. LUCA.
GeneTreeiENSGT00530000062967.
HOGENOMiHOG000116417.
HOVERGENiHBG059832.
InParanoidiQ9D0C1.
KOiK11982.
OMAiCKGEVNP.
OrthoDBiEOG091G0SOO.
PhylomeDBiQ9D0C1.
TreeFamiTF317985.

Family and domain databases

CDDicd00162. RING. 1 hit.
Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
PfamiView protein in Pfam
PF13639. zf-RING_2. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
PROSITEiView protein in PROSITE
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D0C1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEASAAGAD AGSAVAAHRF FCHFCKGEVN PKLPEYICPR CDSGFIEEVT
60 70 80 90 100
DDSSFLGGGG SRTDNSTATH FAELWDHLDH TMFLQDFRPF LSSNPLDQDN
110 120 130 140 150
RANERGHQTH TDFWGPSRPP RLPMTRRYRS RGSTRPDRSP AIEGIIQQIF
160 170 180 190 200
AGFFANSAIP GSPHPFSWSG MLHSNPGDYA WGQTGLDAIV TQLLGQLENT
210 220 230 240 250
GPPPADKEKI TSLPTVTVTQ EQVNTGLECP VCKEDYTVEE KVRQLPCNHF
260 270 280 290 300
FHSSCIVPWL ELHDTCPVCR KSLNGEDSTR QTQSSEASAS NRFSNDSQLH

DRWTF
Length:305
Mass (Da):33,859
Last modified:June 1, 2001 - v1
Checksum:iDF89F268AFE91625
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40R → G in BAB25607 (PubMed:16141072).Curated1
Sequence conflicti240E → G in AAH23113 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008329 mRNA. Translation: BAB25607.1.
AK011584 mRNA. Translation: BAB27716.1.
BC023113 mRNA. Translation: AAH23113.1.
CCDSiCCDS17647.1.
RefSeqiNP_080682.3. NM_026406.3.
UniGeneiMm.386792.

Genome annotation databases

EnsembliENSMUST00000029740; ENSMUSP00000029740; ENSMUSG00000028098.
GeneIDi67845.
KEGGimmu:67845.
UCSCiuc008qoe.2. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRN115_MOUSE
AccessioniPrimary (citable) accession number: Q9D0C1
Secondary accession number(s): Q8R5A1, Q9D885
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: June 1, 2001
Last modified: July 5, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways