Q9D0B0 (SRSF9_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/arginine-rich splicing factor 9

Alternative name(s):
Splicing factor, arginine/serine-rich 9

Gene names

Name:	Srsf9
Synonyms:	Sfrs9

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in constitutive splicing and can modulate the selection of alternative splice sites. Represses the splicing of MAPT/Tau exon 10 By similarity.
Subunit structure	Interacts with NOL3/ARC/NOP30, NSEP1/YB-1/YB1, SAFB/SAFB1, SRSF6/SFRS6, TRA2B/SFRS10 and C1QBP. May also interact with DUSP11/PIR1 By similarity. Interacts with KHDRBS3/SLM-2. Ref.3
Subcellular location	Nucleus By similarity.
Post-translational modification	Extensively phosphorylated on serine residues in the RS domain By similarity.
Sequence similarities	Belongs to the splicing factor SR family. Contains 2 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Domain	Repeat
Ligand	RNA-binding
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of mRNA splicing, via spliceosome Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nucleolus Inferred from electronic annotation. Source: Compara
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 222

222

Serine/arginine-rich splicing factor 9

PRO_0000081936

Regions

Domain

15 – 90

76

RRM 1

Domain

112 – 188

77

RRM 2

Region

189 – 201

13

Interacts with SAFB1 By similarity

Compositional bias

92 – 101

10

Gly-rich (hinge region)

Compositional bias

189 – 201

13

Arg/Ser-rich (RS domain)

Amino acid modifications

Modified residue

190

1

Phosphoserine By similarity

Modified residue

193

1

Phosphotyrosine By similarity

Modified residue

194

1

Phosphoserine By similarity

Modified residue

196

1

Phosphoserine By similarity

Modified residue

205

1

Phosphoserine Ref.6

Modified residue

209

1

Phosphoserine By similarity

Modified residue

212

1

Phosphoserine Ref.4 Ref.5 Ref.6

Modified residue

217

1

Phosphoserine Ref.5 Ref.6

Experimental info

Sequence conflict

209

1

S → N in BAB31986. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

222

Helix Strand Turn

Details...

Beta strand

113 – 117

5

Helix

125 – 132

8

Beta strand

138 – 144

7

Turn

145 – 147

3

Beta strand

148 – 155

8

Helix

156 – 166

11

Beta strand

167 – 172

6

Beta strand

178 – 184

7

Sequences

	Sequence		Length	Mass (Da)
	Q9D0B0 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: DF335337335AED19	FASTA	222	25,661
	10 20 30 40 50 60 MSSGWADERG GEGDGRIYVG NLPSDVREKD LEDLFYKYGR IREIELKNRH GLVPFAFVRF 70 80 90 100 110 120 EDPRDAEDAI YGRNGYDYGQ CRLRVEFPRT YGGRGGWPRG ARNGPPTRRS DFRVLVSGLP 130 140 150 160 170 180 PSGSWQDLKD HMREAGDVCY ADVQKDGMGM VEYLRKEDME YALRKLDDTK FRSHEGETSY 190 200 210 220 IRVYPERSTS YGYSRSRSGS RGRDSPYQSR GSPHYFSPFR PY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo and Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[3]	"The STAR/GSG family protein rSLM-2 regulates the selection of alternative splice sites." Stoss O., Olbrich M., Hartmann A.M., Koenig H., Memmott J., Andreadis A., Stamm S. J. Biol. Chem. 276:8665-8673(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH KHDRBS3.
[4]	"A differential phosphoproteomic analysis of retinoic acid-treated P19 cells." Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D. J. Proteome Res. 6:3174-3186(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-212, MASS SPECTROMETRY. Tissue: Teratocarcinoma.
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-212 AND SER-217, MASS SPECTROMETRY. Tissue: Liver.
[6]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-205; SER-212 AND SER-217, MASS SPECTROMETRY. Tissue: Melanoma.
[7]	"Solution structure of RRM domain in protein BAB31986." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 104-188.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK011621 mRNA. Translation: BAB27740.1.
AK020084 mRNA. Translation: BAB31986.1.
BC012217 mRNA. Translation: AAH12217.1.

IPI

IPI00132340.

RefSeq

NP_079849.1. NM_025573.3.

UniGene

Mm.287826.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WG4	NMR	-	A	104-188	[»]

ProteinModelPortal

Q9D0B0.

SMR

Q9D0B0. Positions 10-98, 105-189.

ModBase

Search...

PTM databases

PhosphoSite

Q9D0B0.

Proteomic databases

PaxDb

Q9D0B0.

PRIDE

Q9D0B0.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000031513; ENSMUSP00000031513; ENSMUSG00000029538.

GeneID

108014.

KEGG

mmu:108014.

UCSC

uc008zdq.1. mouse.

Organism-specific databases

CTD

8683.

MGI

MGI:104896. Srsf9.

Phylogenomic databases

eggNOG

COG0724.

GeneTree

ENSGT00700000104103.

HOVERGEN

HBG002295.

InParanoid

Q9D0B0.

KO

K12890.

OMA

DIREHEI.

Gene expression databases

ArrayExpress

Q9D0B0.

Bgee

Q9D0B0.

CleanEx

MM_SFRS9.

Genevestigator

Q9D0B0.

GermOnline

ENSMUSG00000029538. Mus musculus.

Family and domain databases

Gene3D

3.30.70.330. 2 hits.

InterPro

IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]

Pfam

PF00076. RRM_1. 2 hits.
[Graphical view]

SMART

SM00360. RRM. 2 hits.
[Graphical view]

PROSITE

PS50102. RRM. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9D0B0.

NextBio

359885.

SOURCE

Search...

Entry information

Entry name

SRSF9_MOUSE

Accession

Primary (citable) accession number: Q9D0B0
Secondary accession number(s): Q9CRN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program