ID   ALG14_MOUSE             Reviewed;         217 AA.
AC   Q9D081; Q8CEP2;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   27-MAR-2024, entry version 129.
DE   RecName: Full=UDP-N-acetylglucosamine transferase subunit ALG14 homolog;
GN   Name=Alg14;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Head;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J, and FVB/N; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Kidney, and Pancreas;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Involved in protein N-glycosylation. Essential for the second
CC       step of the dolichol-linked oligosaccharide pathway. Anchors the
CC       catalytic subunit ALG13 to the ER (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer with ALG13 to form a functional enzyme.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:P38242}; Single-pass membrane protein
CC       {ECO:0000255}. Nucleus membrane {ECO:0000250|UniProtKB:P38242}; Single-
CC       pass membrane protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the ALG14 family. {ECO:0000305}.
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DR   EMBL; AK011733; BAB27807.1; -; mRNA.
DR   EMBL; AK017354; BAC25513.1; -; mRNA.
DR   EMBL; BC002278; AAH02278.1; -; mRNA.
DR   CCDS; CCDS17801.1; -.
DR   RefSeq; NP_077140.1; NM_024178.2.
DR   AlphaFoldDB; Q9D081; -.
DR   SMR; Q9D081; -.
DR   BioGRID; 211721; 1.
DR   STRING; 10090.ENSMUSP00000038387; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   iPTMnet; Q9D081; -.
DR   PhosphoSitePlus; Q9D081; -.
DR   MaxQB; Q9D081; -.
DR   PaxDb; 10090-ENSMUSP00000038387; -.
DR   PeptideAtlas; Q9D081; -.
DR   ProteomicsDB; 296193; -.
DR   Pumba; Q9D081; -.
DR   Antibodypedia; 53384; 89 antibodies from 19 providers.
DR   DNASU; 66789; -.
DR   Ensembl; ENSMUST00000039442.12; ENSMUSP00000038387.8; ENSMUSG00000039887.12.
DR   GeneID; 66789; -.
DR   KEGG; mmu:66789; -.
DR   UCSC; uc008rdx.1; mouse.
DR   AGR; MGI:1914039; -.
DR   CTD; 199857; -.
DR   MGI; MGI:1914039; Alg14.
DR   VEuPathDB; HostDB:ENSMUSG00000039887; -.
DR   eggNOG; KOG3339; Eukaryota.
DR   GeneTree; ENSGT00390000002579; -.
DR   HOGENOM; CLU_064541_2_0_1; -.
DR   InParanoid; Q9D081; -.
DR   OMA; SFCRVKS; -.
DR   OrthoDB; 276213at2759; -.
DR   PhylomeDB; Q9D081; -.
DR   TreeFam; TF105628; -.
DR   Reactome; R-MMU-446193; Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
DR   BioGRID-ORCS; 66789; 23 hits in 78 CRISPR screens.
DR   PRO; PR:Q9D081; -.
DR   Proteomes; UP000000589; Chromosome 3.
DR   RNAct; Q9D081; Protein.
DR   Bgee; ENSMUSG00000039887; Expressed in sciatic nerve and 243 other cell types or tissues.
DR   ExpressionAtlas; Q9D081; baseline and differential.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043541; C:UDP-N-acetylglucosamine transferase complex; IBA:GO_Central.
DR   GO; GO:0006488; P:dolichol-linked oligosaccharide biosynthetic process; IBA:GO_Central.
DR   Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 1.
DR   InterPro; IPR013969; Oligosacch_biosynth_Alg14.
DR   PANTHER; PTHR12154; GLYCOSYL TRANSFERASE-RELATED; 1.
DR   PANTHER; PTHR12154:SF4; UDP-N-ACETYLGLUCOSAMINE TRANSFERASE SUBUNIT ALG14 HOMOLOG; 1.
DR   Pfam; PF08660; Alg14; 1.
DR   SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
DR   Genevisible; Q9D081; MM.
PE   1: Evidence at protein level;
KW   Endoplasmic reticulum; Membrane; Nucleus; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..217
FT                   /note="UDP-N-acetylglucosamine transferase subunit ALG14
FT                   homolog"
FT                   /id="PRO_0000265117"
FT   TOPO_DOM        1..3
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250|UniProtKB:P38242"
FT   TRANSMEM        4..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        27..217
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P38242"
SQ   SEQUENCE   217 AA;  24429 MW;  471268EC8AF6BF15 CRC64;
     MLSILILAAT AAGLVILLFQ RLWTVLGPHH VTPRESLRLL IVAGSGGHTT EILRLVGSLS
     NAYSPRHYVI AESDEMSAKK IHSLEELSRA QNDSTTEYPK YHLHRIPRSR EVRQSWLSSV
     FTTFYSMWFS FPLVLRIKPD LVLCNGPGTC VPICVSALLL GILGVKKVII VYVESICRVE
     TLSLSGKILR HLSDYFIVQW PTLKEKYPKS VYLGRIV
//