Q9D032 (SSBP3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Single-stranded DNA-binding protein 3

Alternative name(s):
Lck-associated signal transducer
Sequence-specific single-stranded-DNA-binding protein

Gene names

Name:	Ssbp3
Synonyms:	Last, Ssdp1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in transcription regulation of the alpha 2(I) collagen gene where it binds to the single-stranded polypyrimidine sequences in the promoter region By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Contains 1 LisH domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	head morphogenesis Inferred from mutant phenotype PubMed 15857913. Source: UniProtKB midbrain-hindbrain boundary initiation Inferred from mutant phenotype PubMed 15857913. Source: UniProtKB negative regulation of apoptotic process Inferred from mutant phenotype PubMed 15857913. Source: UniProtKB positive regulation of anterior head development Inferred from mutant phenotype PubMed 15857913. Source: UniProtKB positive regulation of cell proliferation Inferred from mutant phenotype PubMed 15857913. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 15857913. Source: UniProtKB prechordal plate formation Inferred from mutant phenotype PubMed 15857913. Source: UniProtKB protein complex assembly Inferred from mutant phenotype PubMed 15857913. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell protein complex Inferred from direct assay PubMed 15857913. Source: UniProtKB
Molecular_function	single-stranded DNA binding Inferred from sequence alignment PubMed 10524251. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9D032-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9D032-2)

The sequence of this isoform differs from the canonical sequence as follows:
     122-148: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Single-stranded DNA-binding protein 3		PRO_0000123829
Regions
	Domain	16 – 48	33	LisH
	Compositional bias	98 – 319	222	Pro-rich
	Compositional bias	158 – 369	212	Gly-rich
Amino acid modifications
	Modified residue	347	1	Phosphoserine By similarity
	Modified residue	352	1	Phosphoserine By similarity
	Modified residue	360	1	Phosphothreonine By similarity
	Modified residue	381	1	Phosphoserine By similarity
	Modified residue	387	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	122 – 148	27	Missing in isoform 2.		VSP_006262
Experimental info
	Sequence conflict	243	1	A → D in AAG43403. Ref.1
	Sequence conflict	243	1	A → V in BAB27882. Ref.4
	Sequence conflict	335	1	L → V in AAG43403. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 16, 2004. Version 2. Checksum: DBBB169D4EE10536	FASTA	388	40,421
	10 20 30 40 50 60 MFAKGKGSAV PSDGQAREKL ALYVYEYLLH VGAQKSAQTF LSEIRWEKNI TLGEPPGFLH 70 80 90 100 110 120 SWWCVFWDLY CAAPERRDTC EHSSEAKAFH DYSAAAAPSP VLGNIPPNDG MPGGPIPPGF 130 140 150 160 170 180 FQGPPGSQPS PHAQPPPHNP SSMMGPHSQP FMSPRYAGGP RPPIRMGNQP PGGVPGTQPL 190 200 210 220 230 240 LPNSMDPTRQ QGHPNMGGSM QRMNPPRGMG PMGPGPQNYG SGMRPPPNSL GPAMPGINMG 250 260 270 280 290 300 PGAGRPWPNP NSANSIPYSS SSPGTYVGPP GGGGPPGTPI MPSPADSTNS SDNIYTMINP 310 320 330 340 350 360 VPPGGSRSNF PMGPGSDGPM GGMGGMEPHH MNGSLGSGDI DGLPKNSPNN ISGISNPPGT 370 380 PRDDGELGGN FLHSFQNDNY SPSMTMSV
	Isoform 2 [UniParc]. Checksum: CECDA59F18EFC0B2	FASTA	361	37,697
	10 20 30 40 50 60 MFAKGKGSAV PSDGQAREKL ALYVYEYLLH VGAQKSAQTF LSEIRWEKNI TLGEPPGFLH 70 80 90 100 110 120 SWWCVFWDLY CAAPERRDTC EHSSEAKAFH DYSAAAAPSP VLGNIPPNDG MPGGPIPPGF 130 140 150 160 170 180 FQPFMSPRYA GGPRPPIRMG NQPPGGVPGT QPLLPNSMDP TRQQGHPNMG GSMQRMNPPR 190 200 210 220 230 240 GMGPMGPGPQ NYGSGMRPPP NSLGPAMPGI NMGPGAGRPW PNPNSANSIP YSSSSPGTYV 250 260 270 280 290 300 GPPGGGGPPG TPIMPSPADS TNSSDNIYTM INPVPPGGSR SNFPMGPGSD GPMGGMGGME 310 320 330 340 350 360 PHHMNGSLGS GDIDGLPKNS PNNISGISNP PGTPRDDGEL GGNFLHSFQN DNYSPSMTMS V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"LAST, a novel protein binding to the SH2 domain of Lck/Src, mediates signaling events of the Src-type tyrosine kinase." Park C., Choi Y.B., Park D., Hur E.M., Yun Y.D. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: T-cell lymphoma.
[2]	"SSDP1 gene encodes a protein with a conserved N-terminal FORWARD domain." Bayarsaihan D. Biochim. Biophys. Acta 1599:152-155(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"Ssdp proteins bind to LIM-interacting co-factors and regulate the activity of LIM-homeodomain protein complexes in vivo." van Meyel D.J., Thomas J.B., Agulnick A.D. Development 130:1915-1925(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: C3H.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Embryo.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF170906 mRNA. Translation: AAG43403.1. AF500117 mRNA. Translation: AAM22102.1. AY167987 mRNA. Translation: AAN87333.1. AK011853 mRNA. Translation: BAB27882.1. BC003430 mRNA. Translation: AAH03430.1.
IPI	IPI00341944. IPI00623116.
RefSeq	NP_076161.2. NM_023672.2. NP_940840.1. NM_198438.1.
UniGene	Mm.195635. Mm.474861.
3D structure databases
ProteinModelPortal	Q9D032.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-42843N.
MINT	MINT-2568056.
PTM databases
PhosphoSite	Q9D032.
Proteomic databases
PaxDb	Q9D032.
PRIDE	Q9D032.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000030367; ENSMUSP00000030367; ENSMUSG00000061887. ENSMUST00000072753; ENSMUSP00000072536; ENSMUSG00000061887.
GeneID	72475.
KEGG	mmu:72475.
Organism-specific databases
CTD	23648.
MGI	MGI:1919725. Ssbp3.
Phylogenomic databases
eggNOG	NOG245801.
GeneTree	ENSGT00390000009187.
HOGENOM	HOG000037785.
HOVERGEN	HBG068487.
InParanoid	Q9D032.
OMA	HNPNSMM.
OrthoDB	EOG469QW3.
Gene expression databases
ArrayExpress	Q9D032.
Bgee	Q9D032.
CleanEx	MM_SSBP3.
Genevestigator	Q9D032.
GermOnline	ENSMUSG00000061887. Mus musculus.
Family and domain databases
InterPro	IPR006594. LisH_dimerisation. IPR008116. SSDP_DNA-bd. IPR007591. SSDP_ss-bd. [Graphical view]
PANTHER	PTHR12610. PTHR12610. 1 hit.
Pfam	PF04503. SSDP. 1 hit. [Graphical view]
PRINTS	PR01743. SSDNABINDING.
SMART	SM00667. LisH. 1 hit. [Graphical view]
PROSITE	PS50896. LISH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SSBP3. mouse.
NextBio	336302.
SOURCE	Search...

Entry information

Entry name

SSBP3_MOUSE

Accession

Primary (citable) accession number: Q9D032
Secondary accession number(s): Q99LC6, Q9EQP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2003
Last sequence update:	January 16, 2004
Last modified:	April 3, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program