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Q9D030

- TWST2_MOUSE

UniProt

Q9D030 - TWST2_MOUSE

Protein

Twist-related protein 2

Gene

Twist2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Binds to the E-box consensus sequence 5'-CANNTG-3' as a heterodimer and inhibits transcriptional activation by MYOD1, MYOG, MEF2A and MEF2C. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Involved in postnatal glycogen storage and energy metabolism. Inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors By similarity.By similarity

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. DNA binding Source: UniProtKB-KW
    3. protein binding Source: IntAct
    4. protein domain specific binding Source: MGI
    5. RNA polymerase II transcription factor binding transcription factor activity Source: MGI
    6. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. cornea development in camera-type eye Source: MGI
    2. embryonic cranial skeleton morphogenesis Source: MGI
    3. face morphogenesis Source: MGI
    4. negative regulation of cell proliferation Source: MGI
    5. negative regulation of DNA binding Source: MGI
    6. negative regulation of molecular function Source: MGI
    7. negative regulation of myeloid cell differentiation Source: MGI
    8. negative regulation of osteoblast differentiation Source: UniProtKB
    9. negative regulation of transcription, DNA-templated Source: UniProtKB
    10. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    11. negative regulation of tumor necrosis factor production Source: MGI
    12. osteoblast differentiation Source: MGI
    13. positive regulation of keratinocyte proliferation Source: MGI
    14. regulation of transcription from RNA polymerase II promoter Source: MGI
    15. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Developmental protein, Repressor

    Keywords - Biological processi

    Differentiation, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Twist-related protein 2
    Alternative name(s):
    Dermis-expressed protein 1
    Short name:
    Dermo-1
    Gene namesi
    Name:Twist2
    Synonyms:Dermo1
    OrganismiMus musculus (Mouse)Imported
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:104685. Twist2.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation. Cytoplasm 1 Publication
    Note: Mainly nuclear during embryonic development. Cytoplasmic in adult tissues.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi86 – 861F → P: Abrogates transcriptional repression of MYOD1. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 160160Twist-related protein 2PRO_0000127490Add
    BLAST

    Proteomic databases

    PRIDEiQ9D030.

    PTM databases

    PhosphoSiteiQ9D030.

    Expressioni

    Tissue specificityi

    Expressed at low levels in sclerotome and dermatome of somites, and in limb buds at 10.5 dpc. Accumulates predominantly in dermatome, prevertebrae and derivatives of branchial arches by 13 dpc. Also expressed near surface of embryo and in chondrogenic cells. In adult, expressed at low levels in skin, bladder, uterus, aorta and heart.1 Publication

    Developmental stagei

    In the embryo, expression is detected at 10.5 dpc, increases continuously through to 17.5 dpc and is also high in neonates. Down-regulated in adults.1 Publication

    Inductioni

    By TNF-alpha.1 Publication

    Gene expression databases

    CleanExiMM_TWIST2.
    GenevestigatoriQ9D030.

    Interactioni

    Subunit structurei

    Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3/E12. Also interacts with MEF2C.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Runx2Q087752EBI-2903190,EBI-903354

    Protein-protein interaction databases

    BioGridi199209. 1 interaction.
    IntActiQ9D030. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9D030.
    SMRiQ9D030. Positions 67-125.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini66 – 11752bHLHPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The C-terminal and HLH domains are essential for transcriptional repression.1 Publication

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG258515.
    HOGENOMiHOG000261629.
    HOVERGENiHBG019071.
    InParanoidiQ9D030.
    KOiK09069.
    PhylomeDBiQ9D030.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    [Graphical view]
    PfamiPF00010. HLH. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9D030-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEGSSSPVS PVDSLGTSEE ELERQPKRFG RKRRYSKKSS EDGSPTPGKR    50
    GKKGSPSAQS FEELQSQRIL ANVRERQRTQ SLNEAFAALR KIIPTLPSDK 100
    LSKIQTLKLA ARYIDFLYQV LQSDEMDNKM TSCSYVAHER LSYAYSVWRM 150
    EGAWSMSASH 160
    Length:160
    Mass (Da):18,140
    Last modified:June 1, 2001 - v1
    Checksum:i6394750916940C12
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti145 – 1451Y → F in BAB27885. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U36384 mRNA. Translation: AAC52319.1.
    AK011861 mRNA. Translation: BAB27885.1.
    AK011180 mRNA. Translation: BAB27450.1.
    RefSeqiNP_031881.1. NM_007855.2.
    UniGeneiMm.9474.

    Genome annotation databases

    GeneIDi13345.
    KEGGimmu:13345.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U36384 mRNA. Translation: AAC52319.1 .
    AK011861 mRNA. Translation: BAB27885.1 .
    AK011180 mRNA. Translation: BAB27450.1 .
    RefSeqi NP_031881.1. NM_007855.2.
    UniGenei Mm.9474.

    3D structure databases

    ProteinModelPortali Q9D030.
    SMRi Q9D030. Positions 67-125.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199209. 1 interaction.
    IntActi Q9D030. 1 interaction.

    PTM databases

    PhosphoSitei Q9D030.

    Proteomic databases

    PRIDEi Q9D030.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 13345.
    KEGGi mmu:13345.

    Organism-specific databases

    CTDi 117581.
    MGIi MGI:104685. Twist2.

    Phylogenomic databases

    eggNOGi NOG258515.
    HOGENOMi HOG000261629.
    HOVERGENi HBG019071.
    InParanoidi Q9D030.
    KOi K09069.
    PhylomeDBi Q9D030.

    Miscellaneous databases

    NextBioi 283658.
    PROi Q9D030.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_TWIST2.
    Genevestigatori Q9D030.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    [Graphical view ]
    Pfami PF00010. HLH. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Dermo-1: a novel twist-related bHLH protein expressed in the developing dermis."
      Li L., Cserjesi P., Olson E.N.
      Dev. Biol. 172:280-292(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBUNIT.
      Strain: Swiss1 Publication.
      Tissue: Brain1 Publication and Embryonic head1 Publication.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo.
    3. "Dermo-1, a multifunctional basic helix-loop-helix protein, represses MyoD transactivation via the HLH domain, MEF2 interaction, and chromatin deacetylation."
      Gong X.Q., Li L.
      J. Biol. Chem. 277:12310-12317(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF PHE-86.
    4. "Twist regulates cytokine gene expression through a negative feedback loop that represses NF-kappaB activity."
      Sosic D., Richardson J.A., Yu K., Ornitz D.M., Olson E.N.
      Cell 112:169-180(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.

    Entry informationi

    Entry nameiTWST2_MOUSE
    AccessioniPrimary (citable) accession number: Q9D030
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2003
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3