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Q9D030

- TWST2_MOUSE

UniProt

Q9D030 - TWST2_MOUSE

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Protein

Twist-related protein 2

Gene
Twist2, Dermo1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Binds to the E-box consensus sequence 5'-CANNTG-3' as a heterodimer and inhibits transcriptional activation by MYOD1, MYOG, MEF2A and MEF2C. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Involved in postnatal glycogen storage and energy metabolism. Inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors By similarity.3 Publications

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. DNA binding Source: UniProtKB-KW
  3. protein binding Source: IntAct
  4. protein domain specific binding Source: MGI
  5. RNA polymerase II transcription factor binding transcription factor activity Source: MGI
  6. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. cornea development in camera-type eye Source: MGI
  2. embryonic cranial skeleton morphogenesis Source: MGI
  3. face morphogenesis Source: MGI
  4. negative regulation of cell proliferation Source: MGI
  5. negative regulation of DNA binding Source: MGI
  6. negative regulation of molecular function Source: MGI
  7. negative regulation of myeloid cell differentiation Source: MGI
  8. negative regulation of osteoblast differentiation Source: UniProtKB
  9. negative regulation of transcription, DNA-templated Source: UniProtKB
  10. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  11. negative regulation of tumor necrosis factor production Source: MGI
  12. osteoblast differentiation Source: MGI
  13. positive regulation of keratinocyte proliferation Source: MGI
  14. regulation of transcription from RNA polymerase II promoter Source: MGI
  15. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Twist-related protein 2
Alternative name(s):
Dermis-expressed protein 1
Short name:
Dermo-1
Gene namesi
Name:Twist2
Synonyms:Dermo1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:104685. Twist2.

Subcellular locationi

Nucleus. Cytoplasm
Note: Mainly nuclear during embryonic development. Cytoplasmic in adult tissues.1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
  3. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi86 – 861F → P: Abrogates transcriptional repression of MYOD1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 160160Twist-related protein 2PRO_0000127490Add
BLAST

Proteomic databases

PRIDEiQ9D030.

PTM databases

PhosphoSiteiQ9D030.

Expressioni

Tissue specificityi

Expressed at low levels in sclerotome and dermatome of somites, and in limb buds at 10.5 dpc. Accumulates predominantly in dermatome, prevertebrae and derivatives of branchial arches by 13 dpc. Also expressed near surface of embryo and in chondrogenic cells. In adult, expressed at low levels in skin, bladder, uterus, aorta and heart.1 Publication

Developmental stagei

In the embryo, expression is detected at 10.5 dpc, increases continuously through to 17.5 dpc and is also high in neonates. Down-regulated in adults.1 Publication

Inductioni

By TNF-alpha.1 Publication

Gene expression databases

CleanExiMM_TWIST2.
GenevestigatoriQ9D030.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3/E12. Also interacts with MEF2C.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Runx2Q087752EBI-2903190,EBI-903354

Protein-protein interaction databases

BioGridi199209. 1 interaction.
IntActiQ9D030. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9D030.
SMRiQ9D030. Positions 67-125.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini66 – 11752bHLHAdd
BLAST

Domaini

The C-terminal and HLH domains are essential for transcriptional repression.1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG258515.
HOGENOMiHOG000261629.
HOVERGENiHBG019071.
InParanoidiQ9D030.
KOiK09069.
PhylomeDBiQ9D030.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9D030-1 [UniParc]FASTAAdd to Basket

« Hide

MEEGSSSPVS PVDSLGTSEE ELERQPKRFG RKRRYSKKSS EDGSPTPGKR    50
GKKGSPSAQS FEELQSQRIL ANVRERQRTQ SLNEAFAALR KIIPTLPSDK 100
LSKIQTLKLA ARYIDFLYQV LQSDEMDNKM TSCSYVAHER LSYAYSVWRM 150
EGAWSMSASH 160
Length:160
Mass (Da):18,140
Last modified:June 1, 2001 - v1
Checksum:i6394750916940C12
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti145 – 1451Y → F in BAB27885. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U36384 mRNA. Translation: AAC52319.1.
AK011861 mRNA. Translation: BAB27885.1.
AK011180 mRNA. Translation: BAB27450.1.
RefSeqiNP_031881.1. NM_007855.2.
UniGeneiMm.9474.

Genome annotation databases

GeneIDi13345.
KEGGimmu:13345.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U36384 mRNA. Translation: AAC52319.1 .
AK011861 mRNA. Translation: BAB27885.1 .
AK011180 mRNA. Translation: BAB27450.1 .
RefSeqi NP_031881.1. NM_007855.2.
UniGenei Mm.9474.

3D structure databases

ProteinModelPortali Q9D030.
SMRi Q9D030. Positions 67-125.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199209. 1 interaction.
IntActi Q9D030. 1 interaction.

PTM databases

PhosphoSitei Q9D030.

Proteomic databases

PRIDEi Q9D030.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13345.
KEGGi mmu:13345.

Organism-specific databases

CTDi 117581.
MGIi MGI:104685. Twist2.

Phylogenomic databases

eggNOGi NOG258515.
HOGENOMi HOG000261629.
HOVERGENi HBG019071.
InParanoidi Q9D030.
KOi K09069.
PhylomeDBi Q9D030.

Miscellaneous databases

NextBioi 283658.
PROi Q9D030.
SOURCEi Search...

Gene expression databases

CleanExi MM_TWIST2.
Genevestigatori Q9D030.

Family and domain databases

Gene3Di 4.10.280.10. 1 hit.
InterProi IPR011598. bHLH_dom.
[Graphical view ]
Pfami PF00010. HLH. 1 hit.
[Graphical view ]
SMARTi SM00353. HLH. 1 hit.
[Graphical view ]
SUPFAMi SSF47459. SSF47459. 1 hit.
PROSITEi PS50888. BHLH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Dermo-1: a novel twist-related bHLH protein expressed in the developing dermis."
    Li L., Cserjesi P., Olson E.N.
    Dev. Biol. 172:280-292(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBUNIT.
    Strain: Swiss.
    Tissue: Brain and Embryonic head.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  3. "Dermo-1, a multifunctional basic helix-loop-helix protein, represses MyoD transactivation via the HLH domain, MEF2 interaction, and chromatin deacetylation."
    Gong X.Q., Li L.
    J. Biol. Chem. 277:12310-12317(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF PHE-86.
  4. "Twist regulates cytokine gene expression through a negative feedback loop that represses NF-kappaB activity."
    Sosic D., Richardson J.A., Yu K., Ornitz D.M., Olson E.N.
    Cell 112:169-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.

Entry informationi

Entry nameiTWST2_MOUSE
AccessioniPrimary (citable) accession number: Q9D030
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi