ID   MPLKI_MOUSE             Reviewed;         178 AA.
AC   Q9D011;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   24-JAN-2024, entry version 129.
DE   RecName: Full=M-phase-specific PLK1-interacting protein;
DE   AltName: Full=TTD non-photosensitive 1 protein homolog;
GN   Name=Mplkip; Synonyms=Ttdn1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Embryo, and Pituitary;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   METHYLATION [LARGE SCALE ANALYSIS] AT ARG-36; ARG-58; ARG-67 AND ARG-76,
RP   AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, and Embryo;
RX   PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA   Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA   Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA   Bedford M.T., Comb M.J.;
RT   "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT   methylation.";
RL   Mol. Cell. Proteomics 13:372-387(2014).
CC   -!- FUNCTION: May play a role in maintenance of cell cycle integrity by
CC       regulating mitosis or cytokinesis. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with PLK1; phosphorylation-dependent. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Cytoplasm, cytoskeleton,
CC       microtubule organizing center, centrosome {ECO:0000250}. Note=The
CC       subcellular location is regulated during cell cycle. During interphase
CC       located in the nucleus. During mitosis located at the centrosome and
CC       dispersed in the cytoplasm. During telophase located in the midbody.
CC       Colocalizes with PLK1 at the centrosome in M phase.
CC   -!- PTM: Phosphorylated during mitosis in the cell cycle probably by CDK1.
CC       {ECO:0000250}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AK011919; BAB27916.1; -; mRNA.
DR   EMBL; AK030630; BAC27054.1; -; mRNA.
DR   CCDS; CCDS26253.1; -.
DR   RefSeq; NP_079755.1; NM_025479.5.
DR   AlphaFoldDB; Q9D011; -.
DR   STRING; 10090.ENSMUSP00000059154; -.
DR   iPTMnet; Q9D011; -.
DR   PhosphoSitePlus; Q9D011; -.
DR   jPOST; Q9D011; -.
DR   MaxQB; Q9D011; -.
DR   PaxDb; 10090-ENSMUSP00000059154; -.
DR   ProteomicsDB; 291488; -.
DR   Pumba; Q9D011; -.
DR   DNASU; 66308; -.
DR   Ensembl; ENSMUST00000049744.4; ENSMUSP00000059154.4; ENSMUSG00000012429.10.
DR   GeneID; 66308; -.
DR   KEGG; mmu:66308; -.
DR   UCSC; uc007poa.1; mouse.
DR   AGR; MGI:1913558; -.
DR   CTD; 136647; -.
DR   MGI; MGI:1913558; Mplkip.
DR   VEuPathDB; HostDB:ENSMUSG00000012429; -.
DR   eggNOG; ENOG502S6ND; Eukaryota.
DR   GeneTree; ENSGT00390000002582; -.
DR   HOGENOM; CLU_1510143_0_0_1; -.
DR   InParanoid; Q9D011; -.
DR   OMA; QTTCTGK; -.
DR   OrthoDB; 4496402at2759; -.
DR   PhylomeDB; Q9D011; -.
DR   TreeFam; TF335586; -.
DR   BioGRID-ORCS; 66308; 7 hits in 78 CRISPR screens.
DR   PRO; PR:Q9D011; -.
DR   Proteomes; UP000000589; Chromosome 13.
DR   RNAct; Q9D011; Protein.
DR   Bgee; ENSMUSG00000012429; Expressed in ventricular zone and 204 other cell types or tissues.
DR   ExpressionAtlas; Q9D011; baseline and differential.
DR   GO; GO:0005813; C:centrosome; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR   GO; GO:0030496; C:midbody; ISS:UniProtKB.
DR   GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   InterPro; IPR026618; MPLKIP-like_vertebrate.
DR   InterPro; IPR028265; TTDN1/SICKLE.
DR   PANTHER; PTHR22446:SF3; M-PHASE-SPECIFIC PLK1-INTERACTING PROTEIN; 1.
DR   PANTHER; PTHR22446; UNCHARACTERIZED; 1.
DR   Pfam; PF15502; MPLKIP; 1.
DR   Genevisible; Q9D011; MM.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Cytoplasm; Cytoskeleton; Methylation; Mitosis;
KW   Nucleus; Phosphoprotein; Reference proteome.
FT   CHAIN           1..178
FT                   /note="M-phase-specific PLK1-interacting protein"
FT                   /id="PRO_0000065675"
FT   REGION          1..134
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..16
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        90..121
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         36
FT                   /note="Asymmetric dimethylarginine"
FT                   /evidence="ECO:0007744|PubMed:24129315"
FT   MOD_RES         39
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8TAP9"
FT   MOD_RES         46
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8TAP9"
FT   MOD_RES         50
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8TAP9"
FT   MOD_RES         56
FT                   /note="Omega-N-methylarginine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8TAP9"
FT   MOD_RES         58
FT                   /note="Asymmetric dimethylarginine"
FT                   /evidence="ECO:0007744|PubMed:24129315"
FT   MOD_RES         67
FT                   /note="Asymmetric dimethylarginine"
FT                   /evidence="ECO:0007744|PubMed:24129315"
FT   MOD_RES         76
FT                   /note="Asymmetric dimethylarginine"
FT                   /evidence="ECO:0007744|PubMed:24129315"
FT   MOD_RES         79
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8TAP9"
FT   MOD_RES         81
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8TAP9"
FT   MOD_RES         92
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8TAP9"
FT   MOD_RES         103
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8TAP9"
FT   MOD_RES         114
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8TAP9"
FT   MOD_RES         116
FT                   /note="Omega-N-methylarginine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8TAP9"
FT   MOD_RES         119
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8TAP9"
FT   MOD_RES         123
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8TAP9"
FT   MOD_RES         132
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8TAP9"
SQ   SEQUENCE   178 AA;  19060 MW;  0DA799EDEB988986 CRC64;
     MHRPNFRPPT PPYPSPGIGG WGGGNNFRGA LGGGPRPPSP RDGYGSPHHT PPCGPRARPY
     GSSQSPRHGG NFSGARFGSP SPGGYPGSYS RSPAGSQHQF GYSPGQQQTY PQGSPRTSTP
     FGSGRGREKR MSNELESYFK PSMLEDPWAG LEPVSVVDIS QQYSNTQTFT GKKGRYFS
//