Q9CZY3 (UB2V1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 variant 1
Short name=UEV-1

Alternative name(s):
CROC-1

Gene names

Name:	Ube2v1
Synonyms:	Croc1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation By similarity. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Ref.4
Subunit structure	Heterodimer with UBE2N. Interacts (UBE2V2-UBE2N heterodimer) with the E3 ligase STUB1 (via the U-box domain); the complex has a specific 'Lys-63'-linked polyubiquitination activity. Interacts with TRAF6 By similarity. Ref.4 Ref.5
Subcellular location	Nucleus By similarity. Note: Excluded from the nucleolus By similarity.
Tissue specificity	Ubiquitous. Highly expressed in heart, brain, liver, skeletal msucle, kidney and testis. Detected at lower levels in lung and spleen. Ref.4
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	error-free postreplication DNA repair Inferred from genetic interaction Ref.4. Source: MGI protein K63-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	UBC13-UEV1A complex Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell ubiquitin ligase complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	acid-amino acid ligase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction Ref.4. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9CZY3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9CZY3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     58-99: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 147	146	Ubiquitin-conjugating enzyme E2 variant 1		PRO_0000082601
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	146	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	58 – 99	42	Missing in isoform 2.		VSP_011528
Experimental info
	Sequence conflict	59	1	I → M in AAG22085. Ref.4
	Sequence conflict	66	1	S → G in AAG22085. Ref.4
	Sequence conflict	78	1	A → P in AAG22085. Ref.4
	Sequence conflict	100	1	V → M in AAG22085. Ref.4
	Sequence conflict	108	1	L → P in AAH19372. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: E66E83EEE9D08E92	FASTA	147	16,355
	10 20 30 40 50 60 MAATTGSGVK VPRNFRLLEE LEEGQKGVGD GTVSWGLEDD EDMTLTRWTG MIIGPPRTIY 70 80 90 100 110 120 ENRIYSLKIE CGPKYPEAPP SVRFVTRVNM SGVSSSNGVV DPRATAVLAK WQNSHSIKVI 130 140 LQELRRLMMS KENMKLPQPP EGQCYSN
	Isoform 2 [UniParc]. Checksum: CCCF79601969C719	FASTA	105	11,725
	10 20 30 40 50 60 MAATTGSGVK VPRNFRLLEE LEEGQKGVGD GTVSWGLEDD EDMTLTRWTG MIIGPPRVDP 70 80 90 100 RATAVLAKWQ NSHSIKVILQ ELRRLMMSKE NMKLPQPPEG QCYSN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Embryo.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: NMRI. Tissue: Mammary tumor.
[4]	"Molecular cloning and functional characterization of two murine cDNAs which encode Ubc variants involved in DNA repair and mutagenesis." Franko J., Ashley C., Xiao W. Biochim. Biophys. Acta 1519:70-77(2001) [PubMed: 11406273] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-147 (ISOFORM 1), FUNCTION, INTERACTION WITH UBE2N, TISSUE SPECIFICITY.
[5]	"Chaperoned ubiquitylation -- crystal structures of the CHIP U box E3 ubiquitin ligase and a CHIP-Ubc13-Uev1a complex." Zhang M., Windheim M., Roe S.M., Peggie M., Cohen P., Prodromou C., Pearl L.H. Mol. Cell 20:525-538(2005) [PubMed: 16307917] [Abstract] Cited for: INTERACTION WITH STUB1 AND UBE2N.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK012021 mRNA. Translation: BAB27978.1. AL589870 Genomic DNA. Translation: CAM13830.1. BC003449 mRNA. Translation: AAH03449.1. BC019372 mRNA. Translation: AAH19372.1. AF303829 mRNA. Translation: AAG22085.1.
IPI	IPI00113257. IPI00464127.
RefSeq	NP_075719.1. NM_023230.2.
UniGene	Mm.278783. Mm.360108.
3D structure databases
ProteinModelPortal	Q9CZY3.
SMR	Q9CZY3. Positions 8-146.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9CZY3. 1 interaction.
STRING	Q9CZY3.
PTM databases
PhosphoSite	Q9CZY3.
Proteomic databases
PRIDE	Q9CZY3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000060645; ENSMUSP00000053109; ENSMUSG00000078923. ENSMUST00000109207; ENSMUSP00000104830; ENSMUSG00000078923.
GeneID	66589.
KEGG	mmu:66589.
UCSC	uc007yhq.2. mouse.
Organism-specific databases
CTD	7335.
MGI	MGI:1913839. Ube2v1.
Phylogenomic databases
GeneTree	ENSGT00390000009008.
HOVERGEN	HBG054552.
InParanoid	Q9CZY3.
OMA	DETYLCI.
PhylomeDB	Q9CZY3.
Gene expression databases
Bgee	Q9CZY3.
CleanEx	MM_UBE2V1.
Genevestigator	Q9CZY3.
GermOnline	ENSMUSG00000027694. Mus musculus.
Family and domain databases
InterPro	IPR000608. UBQ-conjugat_E2. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
KO	K10704.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	322088.
SOURCE	Search...

Entry information

Entry name

UB2V1_MOUSE

Accession

Primary (citable) accession number: Q9CZY3
Secondary accession number(s): A2A467, Q8VEB5, Q9ERI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	June 1, 2001
Last modified:	November 16, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program