Q9CZW4 (ACSL3_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 85.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Long-chain-fatty-acid--CoA ligase 3 EC=6.2.1.3 Alternative name(s): Long-chain acyl-CoA synthetase 3 Short name=LACS 3 | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 720 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 has mainly an anabolic role in energy metabolism By similarity. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins) By similarity. Mediates hepatic lipogenesis By similarity. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates By similarity. |
| Catalytic activity | ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA. |
| Cofactor | Magnesium By similarity. |
| Subcellular location | Mitochondrion outer membrane; Single-pass type III membrane protein By similarity. Peroxisome membrane; Single-pass type III membrane protein By similarity. Microsome membrane; Single-pass type III membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type III membrane protein By similarity. |
| Induction | High expression in ob/ob mice (obese) and mice fed at high sucrose diet. Ref.4 |
| Sequence similarities | Belongs to the ATP-dependent AMP-binding enzyme family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 720 | 720 | Long-chain-fatty-acid--CoA ligase 3 | PRO_0000193108 | |||||
Regions | |||||||||
| Transmembrane | 21 – 41 | 21 | Helical; Signal-anchor for type III membrane protein; Potential | ||||||
| Topological domain | 42 – 720 | 679 | Cytoplasmic Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 593 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 683 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 688 | 1 | Phosphothreonine By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 223 | 1 | D → E in BAB28022. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo. |
| [2] | Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary tumor. |
| [4] | "Suppression of long chain acyl-CoA synthetase 3 (ACSL3) decreases hepatic de novo fatty acid synthesis through decreased transcriptional activity." Bu S.Y., Mashek M.T., Mashek D.G. J. Biol. Chem. 284:30474-30483(2009) [PubMed: 19737935] [Abstract] Cited for: INDUCTION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AK012088 mRNA. Translation: BAB28022.1. AK161708 mRNA. Translation: BAE36543.1. CH466548 Genomic DNA. Translation: EDL00440.1. CH466548 Genomic DNA. Translation: EDL00441.1. BC031529 mRNA. Translation: AAH31529.1. |
| IPI | IPI00169772. |
| RefSeq | NP_001028778.2. NM_001033606.2. NP_001129694.1. NM_001136222.1. NP_083093.2. NM_028817.3. |
| UniGene | Mm.276016. |
3D structure databases | |
| ProteinModelPortal | Q9CZW4. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q9CZW4. |
PTM databases | |
| PhosphoSite | Q9CZW4. |
Proteomic databases | |
| PRIDE | Q9CZW4. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000035779; ENSMUSP00000045291; ENSMUSG00000032883. ENSMUST00000142704; ENSMUSP00000121695; ENSMUSG00000032883. |
| GeneID | 74205. |
| KEGG | mmu:74205. |
Organism-specific databases | |
| CTD | 2181. |
| MGI | MGI:1921455. Acsl3. |
Phylogenomic databases | |
| GeneTree | ENSGT00600000084142. |
| HOGENOM | HBG721674. |
| HOVERGEN | HBG106947. |
| InParanoid | Q9CZW4. |
| OrthoDB | EOG4Z8XVW. |
| PhylomeDB | Q9CZW4. |
Enzyme and pathway databases | |
| BRENDA | 6.2.1.3. 3474. |
Gene expression databases | |
| ArrayExpress | Q9CZW4. |
| Bgee | Q9CZW4. |
| Genevestigator | Q9CZW4. |
| GermOnline | ENSMUSG00000032883. Mus musculus. |
Family and domain databases | |
| InterPro | IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view] |
| KO | K01897. |
| Pfam | PF00501. AMP-binding. 1 hit. [Graphical view] |
| PROSITE | PS00455. AMP_BINDING. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 340106. |
| SOURCE | Search... |
Entry information
| Entry name | ACSL3_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q9CZW4 Secondary accession number(s): Q8K1J7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with