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Q9CZW4

- ACSL3_MOUSE

UniProt

Q9CZW4 - ACSL3_MOUSE

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Protein

Long-chain-fatty-acid--CoA ligase 3

Gene

Acsl3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 has mainly an anabolic role in energy metabolism (By similarity). Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins) (By similarity). Mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity).By similarity

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Mg2+By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. long-chain fatty acid-CoA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. brain development Source: Ensembl
  2. fatty acid biosynthetic process Source: Ensembl
  3. long-chain fatty acid import Source: Ensembl
  4. positive regulation of Golgi to plasma membrane protein transport Source: Ensembl
  5. positive regulation of phosphatidylcholine biosynthetic process Source: Ensembl
  6. positive regulation of secretion Source: Ensembl
  7. response to nutrient Source: Ensembl
  8. response to organic cyclic compound Source: Ensembl
  9. very-low-density lipoprotein particle assembly Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.2.1.3. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase 3 (EC:6.2.1.3)
Alternative name(s):
Long-chain acyl-CoA synthetase 3
Short name:
LACS 3
Gene namesi
Name:Acsl3
Synonyms:Acs3, Facl3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1921455. Acsl3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei21 – 4121Helical; Signal-anchor for type III membrane proteinSequence AnalysisAdd
BLAST
Topological domaini42 – 720679CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. Golgi apparatus Source: Ensembl
  3. integral component of membrane Source: UniProtKB-KW
  4. lipid particle Source: Ensembl
  5. mitochondrial outer membrane Source: UniProtKB-KW
  6. perinuclear region of cytoplasm Source: Ensembl
  7. peroxisome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 720720Long-chain-fatty-acid--CoA ligase 3PRO_0000193108Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei683 – 6831PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9CZW4.
PaxDbiQ9CZW4.
PRIDEiQ9CZW4.

PTM databases

PhosphoSiteiQ9CZW4.

Expressioni

Inductioni

High expression in ob/ob mice (obese) and mice fed at high sucrose diet.1 Publication

Gene expression databases

BgeeiQ9CZW4.
ExpressionAtlasiQ9CZW4. baseline and differential.
GenevestigatoriQ9CZW4.

Interactioni

Protein-protein interaction databases

IntActiQ9CZW4. 1 interaction.
MINTiMINT-4117014.
STRINGi10090.ENSMUSP00000121695.

Structurei

3D structure databases

ProteinModelPortaliQ9CZW4.
SMRiQ9CZW4. Positions 135-664.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1022.
GeneTreeiENSGT00690000102168.
HOGENOMiHOG000159459.
HOVERGENiHBG106947.
InParanoidiQ9CZW4.
KOiK01897.
OMAiCDRFIFR.
OrthoDBiEOG7P2XRD.
TreeFamiTF314012.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CZW4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNNHVSSTPS TMKLKQTINP ILLYFIHFII SLYTILTYIP FYFLCESKQE
60 70 80 90 100
KPNQIKAKPV SSKPDSAYRS INSVDGLASV LYPGCDTLDK VFMYAKNKFK
110 120 130 140 150
NKRLLGTREI LNEEDEIQPN GKIFKKVILG HYNWLSYEDV FIRALDFGNG
160 170 180 190 200
LQMLGQKPKA NIAIFCETRA EWMIAAQACF MYNFQLVTLY ATLGGPAIVH
210 220 230 240 250
GLNETEVTNI ITSKELLQTK LKDIVSLVPR LRHIITVDGK PPTWSEFPKG
260 270 280 290 300
VIVHTMAAVQ ALGVKANVEK KAHSKPLPSD IAVIMYTSGS TGIPKGVMIS
310 320 330 340 350
HSNIIASITG MARRIPRLGE EDVYIGYLPL AHVLELSAEL VCLSHGCRIG
360 370 380 390 400
YSSPQTLADQ SSKIKKGSKG DTSVLKPTLM AAVPEIMDRI YKNVMNKVNE
410 420 430 440 450
MSAFQRNLFI LAYNYKMEQI SKGCSTPLCD RFVFRNVRRL LGGNIRLLLC
460 470 480 490 500
GGAPLSATTQ RFMNICFCCP VGQGYGLTES TGAGTITEVW DYNTGRVGAP
510 520 530 540 550
LVCCEIKLKN WEEGGYFNTD KPHPRGEILI GGQNVTMGYY KNEAKTKTDF
560 570 580 590 600
FEDENGQRWL CTGDIGEFDP DGCLKIIDRK KDLVKLQAGE YVSLGKVEAA
610 620 630 640 650
LKNLPLIDNI CAYANSYHSY VIGFVVPNQK ELTELARTKG FKGTWEELCN
660 670 680 690 700
SSEMENEVLK VLSEAAISAS LEKFEIPLKI RLSPDPWTPE TGLVTDAFKL
710 720
KRKELKTHYQ ADIERMYGRK
Length:720
Mass (Da):80,492
Last modified:July 27, 2011 - v2
Checksum:i27F9CCA6961B5BA0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti223 – 2231D → E in BAB28022. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012088 mRNA. Translation: BAB28022.1.
AK161708 mRNA. Translation: BAE36543.1.
CH466548 Genomic DNA. Translation: EDL00440.1.
CH466548 Genomic DNA. Translation: EDL00441.1.
BC031529 mRNA. Translation: AAH31529.1.
CCDSiCCDS15087.1.
RefSeqiNP_001028778.2. NM_001033606.2.
NP_001129694.1. NM_001136222.1.
NP_083093.2. NM_028817.3.
UniGeneiMm.276016.

Genome annotation databases

EnsembliENSMUST00000035779; ENSMUSP00000045291; ENSMUSG00000032883.
ENSMUST00000142704; ENSMUSP00000121695; ENSMUSG00000032883.
GeneIDi74205.
KEGGimmu:74205.
UCSCiuc007bqo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012088 mRNA. Translation: BAB28022.1 .
AK161708 mRNA. Translation: BAE36543.1 .
CH466548 Genomic DNA. Translation: EDL00440.1 .
CH466548 Genomic DNA. Translation: EDL00441.1 .
BC031529 mRNA. Translation: AAH31529.1 .
CCDSi CCDS15087.1.
RefSeqi NP_001028778.2. NM_001033606.2.
NP_001129694.1. NM_001136222.1.
NP_083093.2. NM_028817.3.
UniGenei Mm.276016.

3D structure databases

ProteinModelPortali Q9CZW4.
SMRi Q9CZW4. Positions 135-664.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9CZW4. 1 interaction.
MINTi MINT-4117014.
STRINGi 10090.ENSMUSP00000121695.

PTM databases

PhosphoSitei Q9CZW4.

Proteomic databases

MaxQBi Q9CZW4.
PaxDbi Q9CZW4.
PRIDEi Q9CZW4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000035779 ; ENSMUSP00000045291 ; ENSMUSG00000032883 .
ENSMUST00000142704 ; ENSMUSP00000121695 ; ENSMUSG00000032883 .
GeneIDi 74205.
KEGGi mmu:74205.
UCSCi uc007bqo.2. mouse.

Organism-specific databases

CTDi 2181.
MGIi MGI:1921455. Acsl3.

Phylogenomic databases

eggNOGi COG1022.
GeneTreei ENSGT00690000102168.
HOGENOMi HOG000159459.
HOVERGENi HBG106947.
InParanoidi Q9CZW4.
KOi K01897.
OMAi CDRFIFR.
OrthoDBi EOG7P2XRD.
TreeFami TF314012.

Enzyme and pathway databases

BRENDAi 6.2.1.3. 3474.

Miscellaneous databases

NextBioi 340106.
PROi Q9CZW4.
SOURCEi Search...

Gene expression databases

Bgeei Q9CZW4.
ExpressionAtlasi Q9CZW4. baseline and differential.
Genevestigatori Q9CZW4.

Family and domain databases

InterProi IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
[Graphical view ]
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary tumor.
  4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  5. "Suppression of long chain acyl-CoA synthetase 3 (ACSL3) decreases hepatic de novo fatty acid synthesis through decreased transcriptional activity."
    Bu S.Y., Mashek M.T., Mashek D.G.
    J. Biol. Chem. 284:30474-30483(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiACSL3_MOUSE
AccessioniPrimary (citable) accession number: Q9CZW4
Secondary accession number(s): Q8K1J7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3