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Q9CZW2 (CENPN_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Centromere protein N

Short name=CENP-N
Gene names
Name:Cenpn
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length337 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate By similarity.

Subunit structure

Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS By similarity.

Subcellular location

Nucleus By similarity. Chromosomecentromere By similarity. Chromosomecentromerekinetochore By similarity. Note: Localizes exclusively in the kinetochore domain of centromeres By similarity. Kinetochore-bound levels decrease when cells enter mitosis and increase again when cells exit mitosis By similarity.

Ontologies

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9CZW2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9CZW2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-87: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q9CZW2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     231-250: LDSKITHENTEEKVRVHRVT → CMYALSQSWAWGSCAAKHLC
     251-337: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 337337Centromere protein N
PRO_0000249495

Amino acid modifications

Modified residue2261Phosphoserine By similarity

Natural variations

Alternative sequence1 – 8787Missing in isoform 2.
VSP_020443
Alternative sequence231 – 25020LDSKI…VHRVT → CMYALSQSWAWGSCAAKHLC in isoform 3.
VSP_020444
Alternative sequence251 – 33787Missing in isoform 3.
VSP_020445

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: D3EF2CE4DAC900E8

FASTA33739,240
        10         20         30         40         50         60 
MKENVAEFLR RTILKIPLSE MKSILEAWDF LSEDQLQTIN LKQRKDYLAQ EVILLCEDKR 

        70         80         90        100        110        120 
ASLDDVVLLD IVYTQFHRHQ KLWNVFQMSK EPGEDVDLFD MEQFQSSFKR ILQRALKNVT 

       130        140        150        160        170        180 
VSFRVYEKDS VWIRVAWGTQ YSQPNQYKPT FVVYYPQTPY AFISSCHLKN TVPLLHQALK 

       190        200        210        220        230        240 
VASKHHQIVH LDLRSRHLDS LKAIVFREYN QTCENYSSTT SLQEASLSMC LDSKITHENT 

       250        260        270        280        290        300 
EEKVRVHRVT QETFGTYPQP QLEFAQYKLE TKFKSNIGGG LLADRKEPFR CLVKFSSPHL 

       310        320        330 
LEALKSLAPA GIADAPLSPL LTCIPSKKMN YFKIRDK 

« Hide

Isoform 2 [UniParc].

Checksum: D9FD74D936479A52
Show »

FASTA25028,795
Isoform 3 [UniParc].

Checksum: 2F4DD6FCA6118EF0
Show »

FASTA25029,340

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 7-337 (ISOFORM 3).
Strain: FVB/N.
Tissue: Mammary tumor and Olfactory epithelium.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK012097 mRNA. Translation: BAB28028.1.
BC027119 mRNA. Translation: AAH27119.1.
BC058243 mRNA. Translation: AAH58243.1.
RefSeqNP_082407.1. NM_028131.3.
XP_006531450.1. XM_006531387.1.
UniGeneMm.23185.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9CZW2. 1 interaction.
MINTMINT-5198930.

PTM databases

PhosphoSiteQ9CZW2.

Proteomic databases

PRIDEQ9CZW2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034205; ENSMUSP00000034205; ENSMUSG00000031756. [Q9CZW2-1]
GeneID72155.
KEGGmmu:72155.
UCSCuc009noo.1. mouse. [Q9CZW2-1]

Organism-specific databases

CTD55839.
MGIMGI:1919405. Cenpn.

Phylogenomic databases

eggNOGNOG17730.
GeneTreeENSGT00390000004738.
HOGENOMHOG000015409.
HOVERGENHBG058868.
InParanoidQ9CZW2.
KOK11506.
OMAMTILKAW.
OrthoDBEOG7P8PB4.
PhylomeDBQ9CZW2.
TreeFamTF329714.

Gene expression databases

BgeeQ9CZW2.
CleanExMM_CENPN.
GenevestigatorQ9CZW2.

Family and domain databases

InterProIPR027715. CENP-N.
IPR007902. Chl4/mis15/CENP-N.
[Graphical view]
PANTHERPTHR32250:SF0. PTHR32250:SF0. 1 hit.
PfamPF05238. CENP-N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio335584.
PROQ9CZW2.
SOURCESearch...

Entry information

Entry nameCENPN_MOUSE
AccessionPrimary (citable) accession number: Q9CZW2
Secondary accession number(s): Q6PE73, Q8R2W7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot