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Q9CZW2

- CENPN_MOUSE

UniProt

Q9CZW2 - CENPN_MOUSE

Protein

Centromere protein N

Gene

Cenpn

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate By similarity.By similarity

    GO - Biological processi

    1. centromere complex assembly Source: InterPro
    2. chromosome segregation Source: InterPro
    3. mitotic nuclear division Source: InterPro

    Enzyme and pathway databases

    ReactomeiREACT_198597. Deposition of new CENPA-containing nucleosomes at the centromere.
    REACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_207679. Separation of Sister Chromatids.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Centromere protein N
    Short name:
    CENP-N
    Gene namesi
    Name:Cenpn
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1919405. Cenpn.

    Subcellular locationi

    Nucleus By similarity. Chromosomecentromere By similarity. Chromosomecentromerekinetochore By similarity
    Note: Localizes exclusively in the kinetochore domain of centromeres. Kinetochore-bound levels decrease when cells enter mitosis and increase again when cells exit mitosis.By similarity

    GO - Cellular componenti

    1. condensed chromosome kinetochore Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Centromere, Chromosome, Kinetochore, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 337337Centromere protein NPRO_0000249495Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei226 – 2261PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ9CZW2.

    PTM databases

    PhosphoSiteiQ9CZW2.

    Expressioni

    Gene expression databases

    BgeeiQ9CZW2.
    CleanExiMM_CENPN.
    GenevestigatoriQ9CZW2.

    Interactioni

    Subunit structurei

    Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ9CZW2. 1 interaction.
    MINTiMINT-5198930.

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG17730.
    GeneTreeiENSGT00390000004738.
    HOGENOMiHOG000015409.
    HOVERGENiHBG058868.
    InParanoidiQ9CZW2.
    KOiK11506.
    OMAiMTILKAW.
    OrthoDBiEOG7P8PB4.
    PhylomeDBiQ9CZW2.
    TreeFamiTF329714.

    Family and domain databases

    InterProiIPR027715. CENP-N.
    IPR007902. Chl4/mis15/CENP-N.
    [Graphical view]
    PANTHERiPTHR32250. PTHR32250. 1 hit.
    PfamiPF05238. CENP-N. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9CZW2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKENVAEFLR RTILKIPLSE MKSILEAWDF LSEDQLQTIN LKQRKDYLAQ    50
    EVILLCEDKR ASLDDVVLLD IVYTQFHRHQ KLWNVFQMSK EPGEDVDLFD 100
    MEQFQSSFKR ILQRALKNVT VSFRVYEKDS VWIRVAWGTQ YSQPNQYKPT 150
    FVVYYPQTPY AFISSCHLKN TVPLLHQALK VASKHHQIVH LDLRSRHLDS 200
    LKAIVFREYN QTCENYSSTT SLQEASLSMC LDSKITHENT EEKVRVHRVT 250
    QETFGTYPQP QLEFAQYKLE TKFKSNIGGG LLADRKEPFR CLVKFSSPHL 300
    LEALKSLAPA GIADAPLSPL LTCIPSKKMN YFKIRDK 337
    Length:337
    Mass (Da):39,240
    Last modified:June 1, 2001 - v1
    Checksum:iD3EF2CE4DAC900E8
    GO
    Isoform 2 (identifier: Q9CZW2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-87: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:250
    Mass (Da):28,795
    Checksum:iD9FD74D936479A52
    GO
    Isoform 3 (identifier: Q9CZW2-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         231-250: LDSKITHENTEEKVRVHRVT → CMYALSQSWAWGSCAAKHLC
         251-337: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:250
    Mass (Da):29,340
    Checksum:i2F4DD6FCA6118EF0
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 8787Missing in isoform 2. 1 PublicationVSP_020443Add
    BLAST
    Alternative sequencei231 – 25020LDSKI…VHRVT → CMYALSQSWAWGSCAAKHLC in isoform 3. 1 PublicationVSP_020444Add
    BLAST
    Alternative sequencei251 – 33787Missing in isoform 3. 1 PublicationVSP_020445Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK012097 mRNA. Translation: BAB28028.1.
    BC027119 mRNA. Translation: AAH27119.1.
    BC058243 mRNA. Translation: AAH58243.1.
    CCDSiCCDS22693.1. [Q9CZW2-1]
    RefSeqiNP_082407.1. NM_028131.3. [Q9CZW2-1]
    XP_006531450.1. XM_006531387.1. [Q9CZW2-1]
    UniGeneiMm.23185.

    Genome annotation databases

    EnsembliENSMUST00000034205; ENSMUSP00000034205; ENSMUSG00000031756. [Q9CZW2-1]
    GeneIDi72155.
    KEGGimmu:72155.
    UCSCiuc009noo.1. mouse. [Q9CZW2-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK012097 mRNA. Translation: BAB28028.1 .
    BC027119 mRNA. Translation: AAH27119.1 .
    BC058243 mRNA. Translation: AAH58243.1 .
    CCDSi CCDS22693.1. [Q9CZW2-1 ]
    RefSeqi NP_082407.1. NM_028131.3. [Q9CZW2-1 ]
    XP_006531450.1. XM_006531387.1. [Q9CZW2-1 ]
    UniGenei Mm.23185.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9CZW2. 1 interaction.
    MINTi MINT-5198930.

    PTM databases

    PhosphoSitei Q9CZW2.

    Proteomic databases

    PRIDEi Q9CZW2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034205 ; ENSMUSP00000034205 ; ENSMUSG00000031756 . [Q9CZW2-1 ]
    GeneIDi 72155.
    KEGGi mmu:72155.
    UCSCi uc009noo.1. mouse. [Q9CZW2-1 ]

    Organism-specific databases

    CTDi 55839.
    MGIi MGI:1919405. Cenpn.

    Phylogenomic databases

    eggNOGi NOG17730.
    GeneTreei ENSGT00390000004738.
    HOGENOMi HOG000015409.
    HOVERGENi HBG058868.
    InParanoidi Q9CZW2.
    KOi K11506.
    OMAi MTILKAW.
    OrthoDBi EOG7P8PB4.
    PhylomeDBi Q9CZW2.
    TreeFami TF329714.

    Enzyme and pathway databases

    Reactomei REACT_198597. Deposition of new CENPA-containing nucleosomes at the centromere.
    REACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_207679. Separation of Sister Chromatids.

    Miscellaneous databases

    NextBioi 335584.
    PROi Q9CZW2.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9CZW2.
    CleanExi MM_CENPN.
    Genevestigatori Q9CZW2.

    Family and domain databases

    InterProi IPR027715. CENP-N.
    IPR007902. Chl4/mis15/CENP-N.
    [Graphical view ]
    PANTHERi PTHR32250. PTHR32250. 1 hit.
    Pfami PF05238. CENP-N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 7-337 (ISOFORM 3).
      Strain: FVB/N.
      Tissue: Mammary tumor and Olfactory epithelium.

    Entry informationi

    Entry nameiCENPN_MOUSE
    AccessioniPrimary (citable) accession number: Q9CZW2
    Secondary accession number(s): Q6PE73, Q8R2W7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2006
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3