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Protein

Centromere protein N

Gene

Cenpn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.By similarity

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-MMU-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein N
Short name:
CENP-N
Gene namesi
Name:Cenpn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1919405 Cenpn

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002494951 – 337Centromere protein NAdd BLAST337

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei226PhosphoserineBy similarity1
Modified residuei233PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9CZW2
PRIDEiQ9CZW2

PTM databases

iPTMnetiQ9CZW2
PhosphoSitePlusiQ9CZW2

Expressioni

Gene expression databases

BgeeiENSMUSG00000031756
CleanExiMM_CENPN
ExpressionAtlasiQ9CZW2 baseline and differential
GenevisibleiQ9CZW2 MM

Interactioni

Subunit structurei

Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts directly with CENPA. Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1.By similarity

Protein-protein interaction databases

IntActiQ9CZW2, 1 interactor
MINTiQ9CZW2
STRINGi10090.ENSMUSP00000034205

Structurei

3D structure databases

SMRiQ9CZW2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CENP-N/CHL4 family.Curated

Phylogenomic databases

eggNOGiENOG410IKJC Eukaryota
ENOG4111H2Z LUCA
GeneTreeiENSGT00390000004738
HOGENOMiHOG000015409
HOVERGENiHBG058868
InParanoidiQ9CZW2
KOiK11506
OMAiETFGDYP
OrthoDBiEOG091G0YMK
PhylomeDBiQ9CZW2
TreeFamiTF329714

Family and domain databases

InterProiView protein in InterPro
IPR007902 Chl4/mis15/CENP-N
PANTHERiPTHR12309:SF12 PTHR12309:SF12, 1 hit
PfamiView protein in Pfam
PF05238 CENP-N, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CZW2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKENVAEFLR RTILKIPLSE MKSILEAWDF LSEDQLQTIN LKQRKDYLAQ
60 70 80 90 100
EVILLCEDKR ASLDDVVLLD IVYTQFHRHQ KLWNVFQMSK EPGEDVDLFD
110 120 130 140 150
MEQFQSSFKR ILQRALKNVT VSFRVYEKDS VWIRVAWGTQ YSQPNQYKPT
160 170 180 190 200
FVVYYPQTPY AFISSCHLKN TVPLLHQALK VASKHHQIVH LDLRSRHLDS
210 220 230 240 250
LKAIVFREYN QTCENYSSTT SLQEASLSMC LDSKITHENT EEKVRVHRVT
260 270 280 290 300
QETFGTYPQP QLEFAQYKLE TKFKSNIGGG LLADRKEPFR CLVKFSSPHL
310 320 330
LEALKSLAPA GIADAPLSPL LTCIPSKKMN YFKIRDK
Length:337
Mass (Da):39,240
Last modified:June 1, 2001 - v1
Checksum:iD3EF2CE4DAC900E8
GO
Isoform 2 (identifier: Q9CZW2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-87: Missing.

Note: No experimental confirmation available.
Show »
Length:250
Mass (Da):28,795
Checksum:iD9FD74D936479A52
GO
Isoform 3 (identifier: Q9CZW2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     231-250: LDSKITHENTEEKVRVHRVT → CMYALSQSWAWGSCAAKHLC
     251-337: Missing.

Note: No experimental confirmation available.
Show »
Length:250
Mass (Da):29,340
Checksum:i2F4DD6FCA6118EF0
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0204431 – 87Missing in isoform 2. 1 PublicationAdd BLAST87
Alternative sequenceiVSP_020444231 – 250LDSKI…VHRVT → CMYALSQSWAWGSCAAKHLC in isoform 3. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_020445251 – 337Missing in isoform 3. 1 PublicationAdd BLAST87

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012097 mRNA Translation: BAB28028.1
BC027119 mRNA Translation: AAH27119.1
BC058243 mRNA Translation: AAH58243.1
CCDSiCCDS22693.1 [Q9CZW2-1]
RefSeqiNP_082407.1, NM_028131.3 [Q9CZW2-1]
XP_006531450.1, XM_006531387.3 [Q9CZW2-1]
UniGeneiMm.23185

Genome annotation databases

EnsembliENSMUST00000034205; ENSMUSP00000034205; ENSMUSG00000031756 [Q9CZW2-1]
ENSMUST00000212263; ENSMUSP00000148413; ENSMUSG00000031756 [Q9CZW2-2]
GeneIDi72155
KEGGimmu:72155
UCSCiuc009noo.1 mouse [Q9CZW2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCENPN_MOUSE
AccessioniPrimary (citable) accession number: Q9CZW2
Secondary accession number(s): Q6PE73, Q8R2W7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health