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Protein

Centromere protein N

Gene

Cenpn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate (By similarity).By similarity

GO - Biological processi

  1. centromere complex assembly Source: InterPro
  2. chromosome segregation Source: InterPro
  3. mitotic nuclear division Source: InterPro
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_286371. Deposition of new CENPA-containing nucleosomes at the centromere.
REACT_306375. Mitotic Prometaphase.
REACT_321346. Separation of Sister Chromatids.
REACT_329805. Resolution of Sister Chromatid Cohesion.

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein N
Short name:
CENP-N
Gene namesi
Name:Cenpn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1919405. Cenpn.

Subcellular locationi

Nucleus By similarity. Chromosomecentromere By similarity. Chromosomecentromerekinetochore By similarity
Note: Localizes exclusively in the kinetochore domain of centromeres. Kinetochore-bound levels decrease when cells enter mitosis and increase again when cells exit mitosis.By similarity

GO - Cellular componenti

  1. condensed chromosome kinetochore Source: UniProtKB-SubCell
  2. nucleoplasm Source: MGI
  3. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 337337Centromere protein NPRO_0000249495Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei226 – 2261PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9CZW2.

PTM databases

PhosphoSiteiQ9CZW2.

Expressioni

Gene expression databases

BgeeiQ9CZW2.
CleanExiMM_CENPN.
GenevestigatoriQ9CZW2.

Interactioni

Subunit structurei

Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9CZW2. 1 interaction.
MINTiMINT-5198930.

Family & Domainsi

Phylogenomic databases

eggNOGiNOG17730.
GeneTreeiENSGT00390000004738.
HOGENOMiHOG000015409.
HOVERGENiHBG058868.
InParanoidiQ9CZW2.
KOiK11506.
OMAiMTILKAW.
OrthoDBiEOG7P8PB4.
PhylomeDBiQ9CZW2.
TreeFamiTF329714.

Family and domain databases

InterProiIPR027715. CENP-N.
IPR007902. Chl4/mis15/CENP-N.
[Graphical view]
PANTHERiPTHR32250. PTHR32250. 1 hit.
PfamiPF05238. CENP-N. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CZW2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKENVAEFLR RTILKIPLSE MKSILEAWDF LSEDQLQTIN LKQRKDYLAQ
60 70 80 90 100
EVILLCEDKR ASLDDVVLLD IVYTQFHRHQ KLWNVFQMSK EPGEDVDLFD
110 120 130 140 150
MEQFQSSFKR ILQRALKNVT VSFRVYEKDS VWIRVAWGTQ YSQPNQYKPT
160 170 180 190 200
FVVYYPQTPY AFISSCHLKN TVPLLHQALK VASKHHQIVH LDLRSRHLDS
210 220 230 240 250
LKAIVFREYN QTCENYSSTT SLQEASLSMC LDSKITHENT EEKVRVHRVT
260 270 280 290 300
QETFGTYPQP QLEFAQYKLE TKFKSNIGGG LLADRKEPFR CLVKFSSPHL
310 320 330
LEALKSLAPA GIADAPLSPL LTCIPSKKMN YFKIRDK
Length:337
Mass (Da):39,240
Last modified:June 1, 2001 - v1
Checksum:iD3EF2CE4DAC900E8
GO
Isoform 2 (identifier: Q9CZW2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-87: Missing.

Note: No experimental confirmation available.

Show »
Length:250
Mass (Da):28,795
Checksum:iD9FD74D936479A52
GO
Isoform 3 (identifier: Q9CZW2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     231-250: LDSKITHENTEEKVRVHRVT → CMYALSQSWAWGSCAAKHLC
     251-337: Missing.

Note: No experimental confirmation available.

Show »
Length:250
Mass (Da):29,340
Checksum:i2F4DD6FCA6118EF0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8787Missing in isoform 2. 1 PublicationVSP_020443Add
BLAST
Alternative sequencei231 – 25020LDSKI…VHRVT → CMYALSQSWAWGSCAAKHLC in isoform 3. 1 PublicationVSP_020444Add
BLAST
Alternative sequencei251 – 33787Missing in isoform 3. 1 PublicationVSP_020445Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012097 mRNA. Translation: BAB28028.1.
BC027119 mRNA. Translation: AAH27119.1.
BC058243 mRNA. Translation: AAH58243.1.
CCDSiCCDS22693.1. [Q9CZW2-1]
RefSeqiNP_082407.1. NM_028131.3. [Q9CZW2-1]
XP_006531450.1. XM_006531387.2. [Q9CZW2-1]
UniGeneiMm.23185.

Genome annotation databases

EnsembliENSMUST00000034205; ENSMUSP00000034205; ENSMUSG00000031756. [Q9CZW2-1]
GeneIDi72155.
KEGGimmu:72155.
UCSCiuc009noo.1. mouse. [Q9CZW2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012097 mRNA. Translation: BAB28028.1.
BC027119 mRNA. Translation: AAH27119.1.
BC058243 mRNA. Translation: AAH58243.1.
CCDSiCCDS22693.1. [Q9CZW2-1]
RefSeqiNP_082407.1. NM_028131.3. [Q9CZW2-1]
XP_006531450.1. XM_006531387.2. [Q9CZW2-1]
UniGeneiMm.23185.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9CZW2. 1 interaction.
MINTiMINT-5198930.

PTM databases

PhosphoSiteiQ9CZW2.

Proteomic databases

PRIDEiQ9CZW2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034205; ENSMUSP00000034205; ENSMUSG00000031756. [Q9CZW2-1]
GeneIDi72155.
KEGGimmu:72155.
UCSCiuc009noo.1. mouse. [Q9CZW2-1]

Organism-specific databases

CTDi55839.
MGIiMGI:1919405. Cenpn.

Phylogenomic databases

eggNOGiNOG17730.
GeneTreeiENSGT00390000004738.
HOGENOMiHOG000015409.
HOVERGENiHBG058868.
InParanoidiQ9CZW2.
KOiK11506.
OMAiMTILKAW.
OrthoDBiEOG7P8PB4.
PhylomeDBiQ9CZW2.
TreeFamiTF329714.

Enzyme and pathway databases

ReactomeiREACT_286371. Deposition of new CENPA-containing nucleosomes at the centromere.
REACT_306375. Mitotic Prometaphase.
REACT_321346. Separation of Sister Chromatids.
REACT_329805. Resolution of Sister Chromatid Cohesion.

Miscellaneous databases

NextBioi335584.
PROiQ9CZW2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CZW2.
CleanExiMM_CENPN.
GenevestigatoriQ9CZW2.

Family and domain databases

InterProiIPR027715. CENP-N.
IPR007902. Chl4/mis15/CENP-N.
[Graphical view]
PANTHERiPTHR32250. PTHR32250. 1 hit.
PfamiPF05238. CENP-N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 7-337 (ISOFORM 3).
    Strain: FVB/N.
    Tissue: Mammary tumor and Olfactory epithelium.

Entry informationi

Entry nameiCENPN_MOUSE
AccessioniPrimary (citable) accession number: Q9CZW2
Secondary accession number(s): Q6PE73, Q8R2W7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: June 1, 2001
Last modified: April 1, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.