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Protein

F-box/LRR-repeat protein 20

Gene

Fbxl20

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Isoform 3 regulates neural transmission by binding and ubiquitinating RIMS1, a modulator of presynaptic plasticity.1 Publication

GO - Biological processi

  • behavioral fear response Source: MGI
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box/LRR-repeat protein 20
Alternative name(s):
F-box and leucine-rich repeat protein 20
F-box/LRR-repeat protein 2-like
Gene namesi
Name:Fbxl20
Synonyms:Fbl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1919444. Fbxl20.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Altered electrophysiological synaptic activity, with increased frequency of miniature excitatory postsynaptic currents.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001198711 – 436F-box/LRR-repeat protein 20Add BLAST436

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei417PhosphothreonineCombined sources1
Modified residuei421PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9CZV8.
PeptideAtlasiQ9CZV8.
PRIDEiQ9CZV8.

PTM databases

iPTMnetiQ9CZV8.
PhosphoSitePlusiQ9CZV8.

Expressioni

Tissue specificityi

Highly expressed in brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000020883.
CleanExiMM_FBXL20.
ExpressionAtlasiQ9CZV8. baseline and differential.
GenevisibleiQ9CZV8. MM.

Interactioni

Subunit structurei

Interacts with SKP1 and CUL1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Rims1Q99NE54EBI-1551033,EBI-775541

Protein-protein interaction databases

BioGridi215210. 4 interactors.
IntActiQ9CZV8. 5 interactors.
STRINGi10090.ENSMUSP00000099432.

Structurei

3D structure databases

ProteinModelPortaliQ9CZV8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 68F-boxPROSITE-ProRule annotationAdd BLAST47
Repeati74 – 100LRR 1Add BLAST27
Repeati101 – 126LRR 2Add BLAST26
Repeati127 – 152LRR 3Add BLAST26
Repeati153 – 178LRR 4Add BLAST26
Repeati179 – 204LRR 5Add BLAST26
Repeati205 – 230LRR 6Add BLAST26
Repeati231 – 256LRR 7Add BLAST26
Repeati257 – 282LRR 8Add BLAST26
Repeati283 – 308LRR 9Add BLAST26
Repeati309 – 334LRR 10Add BLAST26
Repeati335 – 363LRR 11Add BLAST29
Repeati364 – 388LRR 12Add BLAST25
Repeati389 – 414LRR 13Add BLAST26

Sequence similaritiesi

Contains 1 F-box domain.PROSITE-ProRule annotation
Contains 13 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG4341. Eukaryota.
ENOG410XQ54. LUCA.
GeneTreeiENSGT00760000119059.
HOGENOMiHOG000230659.
HOVERGENiHBG051586.
InParanoidiQ9CZV8.
KOiK10268.
OMAiIWRSATF.
OrthoDBiEOG091G060Y.
PhylomeDBiQ9CZV8.
TreeFamiTF313434.

Family and domain databases

Gene3Di3.80.10.10. 2 hits.
InterProiIPR001810. F-box_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR006553. Leu-rich_rpt_Cys-con_subtyp.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF13516. LRR_6. 4 hits.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
SM00367. LRR_CC. 12 hits.
[Graphical view]
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CZV8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRDVNGVTK SRFEMFSNSD EAVINKKLPK ELLLRIFSFL DVVTLCRCAQ
60 70 80 90 100
VSRAWNVLAL DGSNWQRIDL FDFQRDIEGR VVENISKRCG GFLRKLSLRG
110 120 130 140 150
CLGVGDNALR TFAQNCRNIE VLSLNGCTKT TDATCTSLSK FCSKLRHLDL
160 170 180 190 200
ASCTSITNMS LKALSEGCPL LEQLNISWCD QVTKDGIQAL VRGCGGLKAL
210 220 230 240 250
FLKGCTQLED EALKYIGAHC PELVTLNLQT CLQITDEGLI TICRGCHKLQ
260 270 280 290 300
SLCASGCSNI TDAILNALGQ NCPRLRILEV ARCSQLTDVG FTTLARNCHE
310 320 330 340 350
LEKMDLEECV QITDSTLIQL SIHCPRLQVL SLSHCELITD DGIRHLGNGA
360 370 380 390 400
CAHDQLEVIE LDNCPLITDA SLEHLKSCHS LERIELYDCQ QITRAGIKRL
410 420 430
RTHLPNIKVH AYFAPVTPPP SVGGSRQRFC RCCIIL
Length:436
Mass (Da):48,396
Last modified:February 5, 2008 - v3
Checksum:i3218F38C02EA1BCE
GO
Isoform 2 (identifier: Q9CZV8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-14: Missing.

Note: No experimental confirmation available.
Show »
Length:422
Mass (Da):46,719
Checksum:iD6E65241BB8191EB
GO
Isoform 3 (identifier: Q9CZV8-3) [UniParc]FASTAAdd to basket
Also known as: Scrapper

The sequence of this isoform differs from the canonical sequence as follows:
     1-14: MRRDVNGVTKSRFE → MAPSRDRLLHFGFKAT

Show »
Length:438
Mass (Da):48,548
Checksum:i305466A743427E36
GO

Sequence cautioni

The sequence BAC29349 differs from that shown. Intron retention.Curated
The sequence BAD90157 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40L → P in BAB28039 (PubMed:16141072).Curated1
Sequence conflicti379 – 381HSL → PSF in BAB28039 (PubMed:16141072).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0383541 – 14Missing in isoform 2. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_0307701 – 14MRRDV…KSRFE → MAPSRDRLLHFGFKAT in isoform 3. 1 PublicationAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF649694 mRNA. Translation: ABU95014.1.
AK012109 mRNA. Translation: BAB28039.1.
AK036217 mRNA. Translation: BAC29349.1. Sequence problems.
AK144786 mRNA. Translation: BAE26066.1.
AK220232 mRNA. Translation: BAD90157.1. Different initiation.
AL591205, AL591209 Genomic DNA. Translation: CAM21260.1.
AL591209, AL591205 Genomic DNA. Translation: CAM20406.1.
CCDSiCCDS25339.1. [Q9CZV8-1]
RefSeqiNP_082425.1. NM_028149.1. [Q9CZV8-1]
XP_006534361.1. XM_006534298.3. [Q9CZV8-3]
XP_006534362.1. XM_006534299.3. [Q9CZV8-4]
UniGeneiMm.218350.

Genome annotation databases

EnsembliENSMUST00000103143; ENSMUSP00000099432; ENSMUSG00000020883. [Q9CZV8-1]
GeneIDi72194.
KEGGimmu:72194.
UCSCiuc007lfm.1. mouse. [Q9CZV8-1]
uc009vah.1. mouse. [Q9CZV8-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF649694 mRNA. Translation: ABU95014.1.
AK012109 mRNA. Translation: BAB28039.1.
AK036217 mRNA. Translation: BAC29349.1. Sequence problems.
AK144786 mRNA. Translation: BAE26066.1.
AK220232 mRNA. Translation: BAD90157.1. Different initiation.
AL591205, AL591209 Genomic DNA. Translation: CAM21260.1.
AL591209, AL591205 Genomic DNA. Translation: CAM20406.1.
CCDSiCCDS25339.1. [Q9CZV8-1]
RefSeqiNP_082425.1. NM_028149.1. [Q9CZV8-1]
XP_006534361.1. XM_006534298.3. [Q9CZV8-3]
XP_006534362.1. XM_006534299.3. [Q9CZV8-4]
UniGeneiMm.218350.

3D structure databases

ProteinModelPortaliQ9CZV8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215210. 4 interactors.
IntActiQ9CZV8. 5 interactors.
STRINGi10090.ENSMUSP00000099432.

PTM databases

iPTMnetiQ9CZV8.
PhosphoSitePlusiQ9CZV8.

Proteomic databases

PaxDbiQ9CZV8.
PeptideAtlasiQ9CZV8.
PRIDEiQ9CZV8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103143; ENSMUSP00000099432; ENSMUSG00000020883. [Q9CZV8-1]
GeneIDi72194.
KEGGimmu:72194.
UCSCiuc007lfm.1. mouse. [Q9CZV8-1]
uc009vah.1. mouse. [Q9CZV8-3]

Organism-specific databases

CTDi84961.
MGIiMGI:1919444. Fbxl20.

Phylogenomic databases

eggNOGiKOG4341. Eukaryota.
ENOG410XQ54. LUCA.
GeneTreeiENSGT00760000119059.
HOGENOMiHOG000230659.
HOVERGENiHBG051586.
InParanoidiQ9CZV8.
KOiK10268.
OMAiIWRSATF.
OrthoDBiEOG091G060Y.
PhylomeDBiQ9CZV8.
TreeFamiTF313434.

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiFbxl20. mouse.
PROiQ9CZV8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020883.
CleanExiMM_FBXL20.
ExpressionAtlasiQ9CZV8. baseline and differential.
GenevisibleiQ9CZV8. MM.

Family and domain databases

Gene3Di3.80.10.10. 2 hits.
InterProiIPR001810. F-box_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR006553. Leu-rich_rpt_Cys-con_subtyp.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF13516. LRR_6. 4 hits.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
SM00367. LRR_CC. 12 hits.
[Graphical view]
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFXL20_MOUSE
AccessioniPrimary (citable) accession number: Q9CZV8
Secondary accession number(s): A7YE80
, Q3UMN2, Q571F7, Q8BZ95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: February 5, 2008
Last modified: November 2, 2016
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.