Reviewed, UniProtKB/Swiss-Prot Q9CZV8 (FXL20_MOUSE)

Names and origin

Protein names

Recommended name:
    F-box/LRR-repeat protein 20
Alternative name(s):
    F-box and leucine-rich repeat protein 20
    F-box/LRR-repeat protein 2-like

Gene names

Name:	Fbxl20
Synonyms:	Fbl2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Isoform 3 regulates neural transmission by binding and ubiquitinating RIMS1, a modulator of presynaptic plasticity. Ref.1
Subunit structure	Interacts with SKP1A and CUL1. Ref.1
Subcellular location	Cytoplasm. Note: Isoform 3 is present at the presynaptic membrane. Ref.1
Tissue specificity	Highly expressed in brain. Ref.1
Disruption phenotype	Altered electrophysiological synaptic activity, with increased frequency of miniature excitatory postsynaptic currents. Ref.1
Sequence similarities	Contains 1 F-box domain. Contains 12 LRR (leucine-rich) repeats.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Leucine-rich repeat Repeat
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Ref.1 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Rims1	Q99NE5	2	EBI-1551033,EBI-775541
RPS27A	P62988	1	EBI-1551033,EBI-413034	From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9CZV8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9CZV8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     277-436: Missing.

Isoform 3 (identifier: Q9CZV8-3)

Also known as: Scrapper;

The sequence of this isoform differs from the canonical sequence as follows:
     1-14: MRRDVNGVTKSRFE → MAPSRDRLLHFGFKAT

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	F-box/LRR-repeat protein 20		PRO_0000119871
Regions
	Domain	22 – 68	47	F-box
	Repeat	90 – 115	26	LRR 1
	Repeat	116 – 141	26	LRR 2
	Repeat	142 – 167	26	LRR 3
	Repeat	168 – 193	26	LRR 4
	Repeat	194 – 219	26	LRR 5
	Repeat	220 – 245	26	LRR 6
	Repeat	246 – 271	26	LRR 7
	Repeat	272 – 297	26	LRR 8
	Repeat	298 – 323	26	LRR 9
	Repeat	324 – 349	26	LRR 10
	Repeat	353 – 377	25	LRR 11
	Repeat	378 – 403	26	LRR 12
Amino acid modifications
	Modified residue	417	1	Phosphothreonine Ref.6 Ref.7
	Modified residue	421	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	1 – 14	14	MRRDV…KSRFE → MAPSRDRLLHFGFKAT in isoform 3.		VSP_030770
	Alternative sequence	277 – 436	160	Missing in isoform 2.		VSP_008968
Experimental info
	Sequence conflict	40	1	L → P in BAB28039. Ref.2
	Sequence conflict	379 – 381	3	HSL → PSF in ABU95014. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 5, 2008. Version 3. Checksum: 3218F38C02EA1BCE	FASTA	436	48,396
	10 20 30 40 50 60 MRRDVNGVTK SRFEMFSNSD EAVINKKLPK ELLLRIFSFL DVVTLCRCAQ VSRAWNVLAL 70 80 90 100 110 120 DGSNWQRIDL FDFQRDIEGR VVENISKRCG GFLRKLSLRG CLGVGDNALR TFAQNCRNIE 130 140 150 160 170 180 VLSLNGCTKT TDATCTSLSK FCSKLRHLDL ASCTSITNMS LKALSEGCPL LEQLNISWCD 190 200 210 220 230 240 QVTKDGIQAL VRGCGGLKAL FLKGCTQLED EALKYIGAHC PELVTLNLQT CLQITDEGLI 250 260 270 280 290 300 TICRGCHKLQ SLCASGCSNI TDAILNALGQ NCPRLRILEV ARCSQLTDVG FTTLARNCHE 310 320 330 340 350 360 LEKMDLEECV QITDSTLIQL SIHCPRLQVL SLSHCELITD DGIRHLGNGA CAHDQLEVIE 370 380 390 400 410 420 LDNCPLITDA SLEHLKSCHS LERIELYDCQ QITRAGIKRL RTHLPNIKVH AYFAPVTPPP 430 SVGGSRQRFC RCCIIL
	Isoform 2. Checksum: 982994F91A265E1E	FASTA	276	30,460
	10 20 30 40 50 60 MRRDVNGVTK SRFEMFSNSD EAVINKKLPK ELLLRIFSFL DVVTLCRCAQ VSRAWNVLAL 70 80 90 100 110 120 DGSNWQRIDL FDFQRDIEGR VVENISKRCG GFLRKLSLRG CLGVGDNALR TFAQNCRNIE 130 140 150 160 170 180 VLSLNGCTKT TDATCTSLSK FCSKLRHLDL ASCTSITNMS LKALSEGCPL LEQLNISWCD 190 200 210 220 230 240 QVTKDGIQAL VRGCGGLKAL FLKGCTQLED EALKYIGAHC PELVTLNLQT CLQITDEGLI 250 260 270 TICRGCHKLQ SLCASGCSNI TDAILNALGQ NCPRLR
	Isoform 3 (Scrapper). Checksum: 305466A743427E36	FASTA	438	48,548
	10 20 30 40 50 60 MAPSRDRLLH FGFKATMFSN SDEAVINKKL PKELLLRIFS FLDVVTLCRC AQVSRAWNVL 70 80 90 100 110 120 ALDGSNWQRI DLFDFQRDIE GRVVENISKR CGGFLRKLSL RGCLGVGDNA LRTFAQNCRN 130 140 150 160 170 180 IEVLSLNGCT KTTDATCTSL SKFCSKLRHL DLASCTSITN MSLKALSEGC PLLEQLNISW 190 200 210 220 230 240 CDQVTKDGIQ ALVRGCGGLK ALFLKGCTQL EDEALKYIGA HCPELVTLNL QTCLQITDEG 250 260 270 280 290 300 LITICRGCHK LQSLCASGCS NITDAILNAL GQNCPRLRIL EVARCSQLTD VGFTTLARNC 310 320 330 340 350 360 HELEKMDLEE CVQITDSTLI QLSIHCPRLQ VLSLSHCELI TDDGIRHLGN GACAHDQLEV 370 380 390 400 410 420 IELDNCPLIT DASLEHLKSC HSLERIELYD CQQITRAGIK RLRTHLPNIK VHAYFAPVTP 430 PPSVGGSRQR FCRCCIIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SCRAPPER-dependent ubiquitination of active zone protein RIM1 regulates synaptic vesicle release." Yao I., Takagi H., Ageta H., Kahyo T., Sato S., Hatanaka K., Fukuda Y., Chiba T., Morone N., Yuasa S., Inokuchi K., Ohtsuka T., Macgregor G.R., Tanaka K., Setou M. Cell 130:943-957(2007) [PubMed: 17803915] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH RIMS1, SKP1 AND CUL1, DISRUPTION PHENOTYPE. Strain: C57BL/6J.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Cerebellum, Embryo and Lung.
[3]	"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Natural killer cell.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-417 AND SER-421, MASS SPECTROMETRY. Tissue: Liver.
[7]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-417, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	EF649694 mRNA. Translation: ABU95014.1. AK012109 mRNA. Translation: BAB28039.1. Different initiation. AK036217 mRNA. Translation: BAC29349.1. AK144786 mRNA. Translation: BAE26066.1. Different initiation. AK220232 mRNA. Translation: BAD90157.1. Different initiation. AL591205, AL591209 Genomic DNA. Translation: CAM21260.1. AL591209, AL591205 Genomic DNA. Translation: CAM20406.1. BC116713 mRNA. Translation: AAI16714.1.
IPI	IPI00406404. IPI00605645. IPI00874641.
RefSeq	NP_082425.1.
UniGene	Mm.218350
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9CZV8. 5 interactions.
STRING	Q9CZV8.
PTM databases
PhosphoSite	Q9CZV8.
Proteomic databases
PRIDE	Q9CZV8.
Genome annotation databases
Ensembl	ENSMUST00000075071; ENSMUSP00000074580; ENSMUSG00000020883; Mus musculus. [Genome view] ENSMUST00000103143; ENSMUSP00000099432; ENSMUSG00000020883; Mus musculus. [Genome view]
GeneID	72194.
KEGG	mmu:72194.
UCSC	uc007lfm.1. mouse.
Organism-specific databases
CTD	72194.
MGI	MGI:1919444. Fbxl20.
Phylogenomic databases
HOVERGEN	Q9CZV8.
Gene expression databases
ArrayExpress	Q9CZV8.
Bgee	Q9CZV8.
CleanEx	MM_FBXL20.
Genevestigator	Q9CZV8.
GermOnline	ENSMUSG00000020883. Mus musculus.
Family and domain databases
InterPro	IPR001810. F-box. IPR006553. Leu-rich_rpt_Cys-con_subtyp. [Graphical view]
Pfam	PF00646. F-box. 1 hit. [Graphical view]
SMART	SM00256. FBOX. 1 hit. SM00367. LRR_CC. 2 hits. [Graphical view]
PROSITE	PS50181. FBOX. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	335667.
SOURCE	Search...

Entry information

Entry name

FXL20_MOUSE

Accession

Primary (citable) accession number: Q9CZV8
Secondary accession number(s): A7YE80 , Q3UMN2, Q571F7, Q8BZ95

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
Last sequence update:	February 5, 2008
Last modified:	October 13, 2009
This is version 70 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)