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Protein

F-box/LRR-repeat protein 20

Gene

Fbxl20

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Isoform 3 regulates neural transmission by binding and ubiquitinating RIMS1, a modulator of presynaptic plasticity.1 Publication

GO - Biological processi

  • behavioral fear response Source: MGI
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
F-box/LRR-repeat protein 20
Alternative name(s):
F-box and leucine-rich repeat protein 20
F-box/LRR-repeat protein 2-like
Gene namesi
Name:Fbxl20
Synonyms:Fbl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1919444. Fbxl20.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Altered electrophysiological synaptic activity, with increased frequency of miniature excitatory postsynaptic currents.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 436436F-box/LRR-repeat protein 20PRO_0000119871Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei417 – 4171PhosphothreonineCombined sources
Modified residuei421 – 4211PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9CZV8.
PaxDbiQ9CZV8.
PeptideAtlasiQ9CZV8.
PRIDEiQ9CZV8.

PTM databases

iPTMnetiQ9CZV8.
PhosphoSiteiQ9CZV8.

Expressioni

Tissue specificityi

Highly expressed in brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000020883.
CleanExiMM_FBXL20.
ExpressionAtlasiQ9CZV8. baseline and differential.
GenevisibleiQ9CZV8. MM.

Interactioni

Subunit structurei

Interacts with SKP1 and CUL1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Rims1Q99NE54EBI-1551033,EBI-775541

Protein-protein interaction databases

BioGridi215210. 4 interactions.
IntActiQ9CZV8. 5 interactions.
STRINGi10090.ENSMUSP00000099432.

Structurei

3D structure databases

ProteinModelPortaliQ9CZV8.
SMRiQ9CZV8. Positions 28-389.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 6847F-boxPROSITE-ProRule annotationAdd
BLAST
Repeati74 – 10027LRR 1Add
BLAST
Repeati101 – 12626LRR 2Add
BLAST
Repeati127 – 15226LRR 3Add
BLAST
Repeati153 – 17826LRR 4Add
BLAST
Repeati179 – 20426LRR 5Add
BLAST
Repeati205 – 23026LRR 6Add
BLAST
Repeati231 – 25626LRR 7Add
BLAST
Repeati257 – 28226LRR 8Add
BLAST
Repeati283 – 30826LRR 9Add
BLAST
Repeati309 – 33426LRR 10Add
BLAST
Repeati335 – 36329LRR 11Add
BLAST
Repeati364 – 38825LRR 12Add
BLAST
Repeati389 – 41426LRR 13Add
BLAST

Sequence similaritiesi

Contains 1 F-box domain.PROSITE-ProRule annotation
Contains 13 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG4341. Eukaryota.
ENOG410XQ54. LUCA.
GeneTreeiENSGT00760000119059.
HOGENOMiHOG000230659.
HOVERGENiHBG051586.
InParanoidiQ9CZV8.
KOiK10268.
OMAiIWRSATF.
OrthoDBiEOG091G060Y.
PhylomeDBiQ9CZV8.
TreeFamiTF313434.

Family and domain databases

Gene3Di3.80.10.10. 2 hits.
InterProiIPR001810. F-box_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR006553. Leu-rich_rpt_Cys-con_subtyp.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF13516. LRR_6. 4 hits.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
SM00367. LRR_CC. 12 hits.
[Graphical view]
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CZV8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRDVNGVTK SRFEMFSNSD EAVINKKLPK ELLLRIFSFL DVVTLCRCAQ
60 70 80 90 100
VSRAWNVLAL DGSNWQRIDL FDFQRDIEGR VVENISKRCG GFLRKLSLRG
110 120 130 140 150
CLGVGDNALR TFAQNCRNIE VLSLNGCTKT TDATCTSLSK FCSKLRHLDL
160 170 180 190 200
ASCTSITNMS LKALSEGCPL LEQLNISWCD QVTKDGIQAL VRGCGGLKAL
210 220 230 240 250
FLKGCTQLED EALKYIGAHC PELVTLNLQT CLQITDEGLI TICRGCHKLQ
260 270 280 290 300
SLCASGCSNI TDAILNALGQ NCPRLRILEV ARCSQLTDVG FTTLARNCHE
310 320 330 340 350
LEKMDLEECV QITDSTLIQL SIHCPRLQVL SLSHCELITD DGIRHLGNGA
360 370 380 390 400
CAHDQLEVIE LDNCPLITDA SLEHLKSCHS LERIELYDCQ QITRAGIKRL
410 420 430
RTHLPNIKVH AYFAPVTPPP SVGGSRQRFC RCCIIL
Length:436
Mass (Da):48,396
Last modified:February 5, 2008 - v3
Checksum:i3218F38C02EA1BCE
GO
Isoform 2 (identifier: Q9CZV8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-14: Missing.

Note: No experimental confirmation available.
Show »
Length:422
Mass (Da):46,719
Checksum:iD6E65241BB8191EB
GO
Isoform 3 (identifier: Q9CZV8-3) [UniParc]FASTAAdd to basket
Also known as: Scrapper

The sequence of this isoform differs from the canonical sequence as follows:
     1-14: MRRDVNGVTKSRFE → MAPSRDRLLHFGFKAT

Show »
Length:438
Mass (Da):48,548
Checksum:i305466A743427E36
GO

Sequence cautioni

The sequence BAC29349 differs from that shown.Intron retention.Curated
The sequence BAD90157 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401L → P in BAB28039 (PubMed:16141072).Curated
Sequence conflicti379 – 3813HSL → PSF in BAB28039 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1414Missing in isoform 2. 1 PublicationVSP_038354Add
BLAST
Alternative sequencei1 – 1414MRRDV…KSRFE → MAPSRDRLLHFGFKAT in isoform 3. 1 PublicationVSP_030770Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF649694 mRNA. Translation: ABU95014.1.
AK012109 mRNA. Translation: BAB28039.1.
AK036217 mRNA. Translation: BAC29349.1. Sequence problems.
AK144786 mRNA. Translation: BAE26066.1.
AK220232 mRNA. Translation: BAD90157.1. Different initiation.
AL591205, AL591209 Genomic DNA. Translation: CAM21260.1.
AL591209, AL591205 Genomic DNA. Translation: CAM20406.1.
CCDSiCCDS25339.1. [Q9CZV8-1]
RefSeqiNP_082425.1. NM_028149.1. [Q9CZV8-1]
XP_006534361.1. XM_006534298.2. [Q9CZV8-3]
XP_006534362.1. XM_006534299.2. [Q9CZV8-4]
UniGeneiMm.218350.

Genome annotation databases

EnsembliENSMUST00000103143; ENSMUSP00000099432; ENSMUSG00000020883. [Q9CZV8-1]
GeneIDi72194.
KEGGimmu:72194.
UCSCiuc007lfm.1. mouse. [Q9CZV8-1]
uc009vah.1. mouse. [Q9CZV8-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF649694 mRNA. Translation: ABU95014.1.
AK012109 mRNA. Translation: BAB28039.1.
AK036217 mRNA. Translation: BAC29349.1. Sequence problems.
AK144786 mRNA. Translation: BAE26066.1.
AK220232 mRNA. Translation: BAD90157.1. Different initiation.
AL591205, AL591209 Genomic DNA. Translation: CAM21260.1.
AL591209, AL591205 Genomic DNA. Translation: CAM20406.1.
CCDSiCCDS25339.1. [Q9CZV8-1]
RefSeqiNP_082425.1. NM_028149.1. [Q9CZV8-1]
XP_006534361.1. XM_006534298.2. [Q9CZV8-3]
XP_006534362.1. XM_006534299.2. [Q9CZV8-4]
UniGeneiMm.218350.

3D structure databases

ProteinModelPortaliQ9CZV8.
SMRiQ9CZV8. Positions 28-389.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215210. 4 interactions.
IntActiQ9CZV8. 5 interactions.
STRINGi10090.ENSMUSP00000099432.

PTM databases

iPTMnetiQ9CZV8.
PhosphoSiteiQ9CZV8.

Proteomic databases

MaxQBiQ9CZV8.
PaxDbiQ9CZV8.
PeptideAtlasiQ9CZV8.
PRIDEiQ9CZV8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103143; ENSMUSP00000099432; ENSMUSG00000020883. [Q9CZV8-1]
GeneIDi72194.
KEGGimmu:72194.
UCSCiuc007lfm.1. mouse. [Q9CZV8-1]
uc009vah.1. mouse. [Q9CZV8-3]

Organism-specific databases

CTDi84961.
MGIiMGI:1919444. Fbxl20.

Phylogenomic databases

eggNOGiKOG4341. Eukaryota.
ENOG410XQ54. LUCA.
GeneTreeiENSGT00760000119059.
HOGENOMiHOG000230659.
HOVERGENiHBG051586.
InParanoidiQ9CZV8.
KOiK10268.
OMAiIWRSATF.
OrthoDBiEOG091G060Y.
PhylomeDBiQ9CZV8.
TreeFamiTF313434.

Miscellaneous databases

ChiTaRSiFbxl20. mouse.
PROiQ9CZV8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020883.
CleanExiMM_FBXL20.
ExpressionAtlasiQ9CZV8. baseline and differential.
GenevisibleiQ9CZV8. MM.

Family and domain databases

Gene3Di3.80.10.10. 2 hits.
InterProiIPR001810. F-box_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR006553. Leu-rich_rpt_Cys-con_subtyp.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF13516. LRR_6. 4 hits.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
SM00367. LRR_CC. 12 hits.
[Graphical view]
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFXL20_MOUSE
AccessioniPrimary (citable) accession number: Q9CZV8
Secondary accession number(s): A7YE80
, Q3UMN2, Q571F7, Q8BZ95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: February 5, 2008
Last modified: September 7, 2016
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.