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Protein

Citrate synthase, mitochondrial

Gene

Cs

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase, mitochondrial (Cs)
  2. Aconitate hydratase, mitochondrial (Aco2)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei301PROSITE-ProRule annotation1
Active sitei347PROSITE-ProRule annotation1
Active sitei402PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

ReactomeiR-MMU-71403. Citric acid cycle (TCA cycle).
UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase, mitochondrial (EC:2.3.3.1)
Alternative name(s):
Citrate (Si)-synthase
Gene namesi
Name:Cs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:88529. Cs.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • mitochondrial matrix Source: UniProtKB
  • mitochondrion Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176798.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 27MitochondrionBy similarityAdd BLAST27
ChainiPRO_000000547228 – 464Citrate synthase, mitochondrialAdd BLAST437

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei57N6-succinyllysineCombined sources1
Modified residuei76N6-acetyllysine; alternateBy similarity1
Modified residuei76N6-succinyllysine; alternateBy similarity1
Modified residuei103N6-succinyllysineCombined sources1
Modified residuei193N6-succinyllysineCombined sources1
Modified residuei226PhosphoserineCombined sources1
Modified residuei321N6-acetyllysine; alternateCombined sources1
Modified residuei321N6-succinyllysine; alternateCombined sources1
Modified residuei327N6-acetyllysine; alternateCombined sources1
Modified residuei327N6-succinyllysine; alternateCombined sources1
Modified residuei375N6-acetyllysine; alternateCombined sources1
Modified residuei375N6-succinyllysine; alternateCombined sources1
Modified residuei382N6-acetyllysineCombined sources1
Modified residuei393N6-acetyllysine; alternateCombined sources1
Modified residuei393N6-succinyllysine; alternateCombined sources1
Modified residuei395N6,N6,N6-trimethyllysineBy similarity1
Modified residuei450N6-succinyllysineCombined sources1
Modified residuei459N6-acetyllysine; alternateCombined sources1
Modified residuei459N6-succinyllysine; alternateCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ9CZU6.
MaxQBiQ9CZU6.
PaxDbiQ9CZU6.
PeptideAtlasiQ9CZU6.
PRIDEiQ9CZU6.

PTM databases

iPTMnetiQ9CZU6.
PhosphoSitePlusiQ9CZU6.
SwissPalmiQ9CZU6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000005683.
CleanExiMM_CS.
GenevisibleiQ9CZU6. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi198926. 2 interactors.
IntActiQ9CZU6. 7 interactors.
MINTiMINT-1844265.
STRINGi10090.ENSMUSP00000005826.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NZIX-ray2.75B371-377[»]
ProteinModelPortaliQ9CZU6.
SMRiQ9CZU6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2617. Eukaryota.
COG0372. LUCA.
GeneTreeiENSGT00390000006813.
HOGENOMiHOG000130831.
HOVERGENiHBG005336.
InParanoidiQ9CZU6.
KOiK01647.
OMAiDYIWKTL.
OrthoDBiEOG091G068F.
PhylomeDBiQ9CZU6.
TreeFamiTF300398.

Family and domain databases

Gene3Di1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01793. cit_synth_euk. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CZU6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLTAATRL LGAKNSSCLV LAARHASASS TNLKDVLSNL IPKEQARIKT
60 70 80 90 100
FKQQHGKTVV GQITVDMMYG GMRGMKGLVY ETSVLDPDEG IRFRGYSIPE
110 120 130 140 150
CQKMLPKAKG GEEPLPEGLF WLLVTGQMPT EEQVSWLSRE WAKRAALPSH
160 170 180 190 200
VVTMLDNFPT NLHPMSQLSA AITALNSESN FARAYAEGMN RAKYWELIYE
210 220 230 240 250
DCMDLIAKLP CVAAKIYRNL YREGSSIGAI DSRLDWSHNF TNMLGYTDPQ
260 270 280 290 300
FTELMRLYLT IHSDHEGGNV SAHTSHLVGS ALSDPYLSFA AAMNGLAGPL
310 320 330 340 350
HGLANQEVLV WLTQLQKEVG KDVSDEKLRD YIWNTLNSGR VVPGYGHAVL
360 370 380 390 400
RKTDPRYSCQ REFALKHLPK DPMFKLVAQL YKIVPNILLE QGKAKNPWPN
410 420 430 440 450
VDAHSGVLLQ YYGMTEMNYY TVLFGVSRAL GVLAQLIWSR ALGFPLERPK
460
SMSTDGLMKF VDSK
Length:464
Mass (Da):51,737
Last modified:June 1, 2001 - v1
Checksum:i710639871C31EED5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056479 mRNA. Translation: BAB63945.1.
AK012151 mRNA. Translation: BAB28063.1.
AK145643 mRNA. Translation: BAE26560.1.
AK149990 mRNA. Translation: BAE29218.1.
AK163273 mRNA. Translation: BAE37268.1.
AK166814 mRNA. Translation: BAE39041.1.
AK167125 mRNA. Translation: BAE39273.1.
BC013554 mRNA. Translation: AAH13554.1.
BC029754 mRNA. Translation: AAH29754.1.
CCDSiCCDS24273.1.
RefSeqiNP_080720.1. NM_026444.4.
UniGeneiMm.58836.

Genome annotation databases

EnsembliENSMUST00000005826; ENSMUSP00000005826; ENSMUSG00000005683.
GeneIDi12974.
KEGGimmu:12974.
UCSCiuc007hmj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056479 mRNA. Translation: BAB63945.1.
AK012151 mRNA. Translation: BAB28063.1.
AK145643 mRNA. Translation: BAE26560.1.
AK149990 mRNA. Translation: BAE29218.1.
AK163273 mRNA. Translation: BAE37268.1.
AK166814 mRNA. Translation: BAE39041.1.
AK167125 mRNA. Translation: BAE39273.1.
BC013554 mRNA. Translation: AAH13554.1.
BC029754 mRNA. Translation: AAH29754.1.
CCDSiCCDS24273.1.
RefSeqiNP_080720.1. NM_026444.4.
UniGeneiMm.58836.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NZIX-ray2.75B371-377[»]
ProteinModelPortaliQ9CZU6.
SMRiQ9CZU6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198926. 2 interactors.
IntActiQ9CZU6. 7 interactors.
MINTiMINT-1844265.
STRINGi10090.ENSMUSP00000005826.

Chemistry databases

ChEMBLiCHEMBL2176798.

PTM databases

iPTMnetiQ9CZU6.
PhosphoSitePlusiQ9CZU6.
SwissPalmiQ9CZU6.

Proteomic databases

EPDiQ9CZU6.
MaxQBiQ9CZU6.
PaxDbiQ9CZU6.
PeptideAtlasiQ9CZU6.
PRIDEiQ9CZU6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005826; ENSMUSP00000005826; ENSMUSG00000005683.
GeneIDi12974.
KEGGimmu:12974.
UCSCiuc007hmj.1. mouse.

Organism-specific databases

CTDi1431.
MGIiMGI:88529. Cs.

Phylogenomic databases

eggNOGiKOG2617. Eukaryota.
COG0372. LUCA.
GeneTreeiENSGT00390000006813.
HOGENOMiHOG000130831.
HOVERGENiHBG005336.
InParanoidiQ9CZU6.
KOiK01647.
OMAiDYIWKTL.
OrthoDBiEOG091G068F.
PhylomeDBiQ9CZU6.
TreeFamiTF300398.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.
ReactomeiR-MMU-71403. Citric acid cycle (TCA cycle).

Miscellaneous databases

ChiTaRSiCs. mouse.
PROiQ9CZU6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005683.
CleanExiMM_CS.
GenevisibleiQ9CZU6. MM.

Family and domain databases

Gene3Di1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01793. cit_synth_euk. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCISY_MOUSE
AccessioniPrimary (citable) accession number: Q9CZU6
Secondary accession number(s): Q3UDP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.