Q9CZN4 (SHSA9_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein shisa-9

Alternative name(s):
Cystine-knot AMPAR modulating protein of 44 kDa
Short name=CKAMP44

Gene names

Name:

Shisa9

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulator of short-term neuronal synaptic plasticity in the dentate gyrus. Associates with AMPA receptors (ionotropic glutamate receptors) in synaptic spines and promotes AMPA receptor desensitization at excitatory synapses. Ref.1
Subunit structure	Component of some AMPA receptors (ionotropic glutamate receptors) complex, at least composed of some AMPA receptor (GRIA1, GRIA2 and/or GRIA3), CACNG2 and SHISA9, as well as low level of DLG4. Ref.1
Subcellular location	Cell projection › dendritic spine membrane; Single-pass type I membrane protein. Cell junction › synapse Ref.1.
Tissue specificity	Brain-specific. Mainly expressed in neurons, including in hippocampus, cerebral cortex, striatum, thalamus, olfactory bulb and cerebellum. Expressed in most brain structures during embryonic and postnatal development. Ref.1
Domain	The extracellular domain contains a pattern of cysteine similar to the snail conotoxin Con-ikot-ikot (AC P0CB20), a toxin known to disrupt AMPA receptors (ionotropic glutamate receptor) desensitization (Ref.1).
Disruption phenotype	Induces an increase in the paired-pulse ratio of AMPA currents in lateral and medial perforant path-granule cell synapses. Ref.1
Sequence similarities	Belongs to the shisa family. SHISA9 subfamily.
Sequence caution	The sequence BAB28201.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Cell projection Membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of short-term neuronal synaptic plasticity Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex Inferred from direct assay PubMed 22632720. Source: MGI cell junction Inferred from electronic annotation. Source: UniProtKB-KW dendritic spine membrane Inferred from direct assay Ref.1. Source: UniProtKB synapse Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9CZN4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9CZN4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     189-194: ALADVM → RIQRSF
     195-424: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Isoform 3 (identifier: Q9CZN4-3)

Also known as: Ckamp44a;

The sequence of this isoform differs from the canonical sequence as follows:
     231-231: Missing.

Note: Produced by aberrant splicing sites.

Isoform 4 (identifier: Q9CZN4-4)

Also known as: Ckamp44b;

The sequence of this isoform differs from the canonical sequence as follows:
     231-231: Missing.
     283-298: Missing.

Note: Produced by aberrant splicing sites.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 424	401	Protein shisa-9		PRO_0000394254
Regions
	Topological domain	24 – 149	126	Extracellular Potential
	Transmembrane	150 – 170	21	Helical; Potential
	Topological domain	171 – 424	254	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	45	1	N-linked (GlcNAc...) Potential
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Glycosylation	116	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	189 – 194	6	ALADVM → RIQRSF in isoform 2.		VSP_039229
	Alternative sequence	195 – 424	230	Missing in isoform 2.		VSP_039230
	Alternative sequence	231	1	Missing in isoform 3 and isoform 4.		VSP_039231
	Alternative sequence	283 – 298	16	Missing in isoform 4.		VSP_039232

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 18, 2010. Version 2. Checksum: 0DE039C1C29E9947	FASTA	424	46,842
	10 20 30 40 50 60 MRRVLRLLLG CFLTELCARM CRAQERSGHG QLAQLGGVLL LTGGNRSGAA SGEAGEGVGG 70 80 90 100 110 120 SDAPPTRAPT PDSCRGYFDV MGQWDPPFNC SSGDFIFCCG TCGFRFCCTF KKRRLNQSTC 130 140 150 160 170 180 TNYDTPLWLN TGKPPARKDD PLHDPTKDKT NLIVYIICGV VAVMVLVGIF TKLGLEKAHR 190 200 210 220 230 240 PQREHMSRAL ADVMRPQGHC NTDHMERDLN IVVHVQHYEN MDSRTPINNL HTTQMNNAVP 250 260 270 280 290 300 TSPLLQQMGH PHSYPNLGQI SNPYEQQPPG KELNKYASLK AVGNSDGDWA VATLKSPKAD 310 320 330 340 350 360 KVNDDFYAKR RHLAELAVKG NLPLHPVRVE DEPRAFSPEH GPAQQNGQKS RTNKMPPHPL 370 380 390 400 410 420 AYNSTANFKT WDPSDQSLRR QAYGNKGKLG IAESGSCDPL GTRTQHFPPT QPYFITNSKT EVTV
	Isoform 2 [UniParc]. Checksum: B800170BC7194735	FASTA	194	21,346
	10 20 30 40 50 60 MRRVLRLLLG CFLTELCARM CRAQERSGHG QLAQLGGVLL LTGGNRSGAA SGEAGEGVGG 70 80 90 100 110 120 SDAPPTRAPT PDSCRGYFDV MGQWDPPFNC SSGDFIFCCG TCGFRFCCTF KKRRLNQSTC 130 140 150 160 170 180 TNYDTPLWLN TGKPPARKDD PLHDPTKDKT NLIVYIICGV VAVMVLVGIF TKLGLEKAHR 190 PQREHMSRRI QRSF
	Isoform 3 (Ckamp44a) [UniParc]. Checksum: 3D3C975C132B6317	FASTA	423	46,705
	10 20 30 40 50 60 MRRVLRLLLG CFLTELCARM CRAQERSGHG QLAQLGGVLL LTGGNRSGAA SGEAGEGVGG 70 80 90 100 110 120 SDAPPTRAPT PDSCRGYFDV MGQWDPPFNC SSGDFIFCCG TCGFRFCCTF KKRRLNQSTC 130 140 150 160 170 180 TNYDTPLWLN TGKPPARKDD PLHDPTKDKT NLIVYIICGV VAVMVLVGIF TKLGLEKAHR 190 200 210 220 230 240 PQREHMSRAL ADVMRPQGHC NTDHMERDLN IVVHVQHYEN MDSRTPINNL TTQMNNAVPT 250 260 270 280 290 300 SPLLQQMGHP HSYPNLGQIS NPYEQQPPGK ELNKYASLKA VGNSDGDWAV ATLKSPKADK 310 320 330 340 350 360 VNDDFYAKRR HLAELAVKGN LPLHPVRVED EPRAFSPEHG PAQQNGQKSR TNKMPPHPLA 370 380 390 400 410 420 YNSTANFKTW DPSDQSLRRQ AYGNKGKLGI AESGSCDPLG TRTQHFPPTQ PYFITNSKTE VTV
	Isoform 4 (Ckamp44b) [UniParc]. Checksum: 1ED919C302DF845C	FASTA	407	45,077
	10 20 30 40 50 60 MRRVLRLLLG CFLTELCARM CRAQERSGHG QLAQLGGVLL LTGGNRSGAA SGEAGEGVGG 70 80 90 100 110 120 SDAPPTRAPT PDSCRGYFDV MGQWDPPFNC SSGDFIFCCG TCGFRFCCTF KKRRLNQSTC 130 140 150 160 170 180 TNYDTPLWLN TGKPPARKDD PLHDPTKDKT NLIVYIICGV VAVMVLVGIF TKLGLEKAHR 190 200 210 220 230 240 PQREHMSRAL ADVMRPQGHC NTDHMERDLN IVVHVQHYEN MDSRTPINNL TTQMNNAVPT 250 260 270 280 290 300 SPLLQQMGHP HSYPNLGQIS NPYEQQPPGK ELNKYASLKA VADKVNDDFY AKRRHLAELA 310 320 330 340 350 360 VKGNLPLHPV RVEDEPRAFS PEHGPAQQNG QKSRTNKMPP HPLAYNSTAN FKTWDPSDQS 370 380 390 400 LRRQAYGNKG KLGIAESGSC DPLGTRTQHF PPTQPYFITN SKTEVTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CKAMP44: A brain-specific protein attenuating short-term synaptic plasticity in the dentate gyrus." von Engelhardt J., Mack V., Sprengel R., Kavenstock N., Li K.W., Stern-Bach Y., Smit A.B., Seeburg P.H., Monyer H. Science 327:1518-1522(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4), FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A AMPA RECEPTOR COMPLEX, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	GU479981 mRNA. Translation: ADD64956.1. GU479982 mRNA. Translation: ADD64957.1. AC154289 Genomic DNA. No translation available. AC129020 Genomic DNA. No translation available. AK012380 mRNA. Translation: BAB28201.1. Different initiation.
IPI	IPI00808044. IPI00956857. IPI00968411. IPI00975182.
RefSeq	NP_082553.2. NM_028277.2.
UniGene	Mm.310139.
3D structure databases
ProteinModelPortal	Q9CZN4.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000023138.
PTM databases
PhosphoSite	Q9CZN4.
Proteomic databases
PaxDb	Q9CZN4.
PRIDE	Q9CZN4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000170672; ENSMUSP00000132646; ENSMUSG00000022494.
GeneID	72555.
KEGG	mmu:72555.
UCSC	uc007yfr.1. mouse. uc007yfs.2. mouse.
Organism-specific databases
CTD	729993.
MGI	MGI:1919805. Shisa9.
Phylogenomic databases
eggNOG	NOG26961.
GeneTree	ENSGT00510000047247.
HOGENOM	HOG000169786.
InParanoid	Q9CZN4.
OMA	GSYLYCC.
OrthoDB	EOG4VHK6X.
Gene expression databases
ArrayExpress	Q9CZN4.
Bgee	Q9CZN4.
Genevestigator	Q9CZN4.
Family and domain databases
InterPro	IPR026910. Shisa. [Graphical view]
Pfam	PF13908. Shisa. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	336479.
SOURCE	Search...

Entry information

Entry name

SHSA9_MOUSE

Accession

Primary (citable) accession number: Q9CZN4
Secondary accession number(s): D4NZG2, D4NZG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 18, 2010
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program