Q9CZM9 (KCMB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calcium-activated potassium channel subunit beta-2

Alternative name(s):
BK channel subunit beta-2
Short name=BKbeta2
Calcium-activated potassium channel, subfamily M subunit beta-2
Charybdotoxin receptor subunit beta-2
K(VCA)beta-2
Maxi K channel subunit beta-2
Slo-beta-2

Gene names

Name:

Kcnmb2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Acts as a negative regulator that confers rapid and complete inactivation of KCNMA1 channel complex By similarity.
Subunit structure	Interacts with KCNMA1 tetramer. There are probably 4 molecules of KCMNB2 per KCNMA1 tetramer By similarity.
Subcellular location	Membrane; Multi-pass membrane protein By similarity.
Domain	The ball and chain domain mediates the inactivation of KCNMA1. It occludes the conduction pathway of KCNMA1 channels, and comprises the pore-blocking ball domain (residues 1-17) and the chain domain (residues 20-45) linking it to the transmembrane segment. The ball domain is made up of a flexible N-terminus anchored at a well ordered loop-helix motif. The chain domain consists of a 4-turn helix with an unfolded linker at its C-terminus By similarity.
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB2 subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ion channel
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	detection of calcium ion Inferred from electronic annotation. Source: Compara regulation of action potential in neuron Inferred from electronic annotation. Source: Compara
Cellular_component	voltage-gated potassium channel complex Inferred from electronic annotation. Source: Compara
Molecular_function	calcium-activated potassium channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	Calcium-activated potassium channel subunit beta-2		PRO_0000187052
Regions
	Topological domain	1 – 46	46	Cytoplasmic Potential
	Transmembrane	47 – 67	21	Helical; Name=1; Potential
	Topological domain	68 – 194	127	Extracellular Potential
	Transmembrane	195 – 215	21	Helical; Name=2; Potential
	Topological domain	216 – 235	20	Cytoplasmic Potential
	Region	1 – 45	45	Ball and chain
Amino acid modifications
	Glycosylation	88	1	N-linked (GlcNAc...) Potential
	Glycosylation	96	1	N-linked (GlcNAc...) Potential
	Glycosylation	119	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9CZM9 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: F87D3D91C22BBDF2	FASTA	235	27,120
	10 20 30 40 50 60 MFIWTSGRTS SSYRQDEKRN IYQKIRDHDL LDKRKTVTAL KAGEDRAILL GLAMMVCSIM 70 80 90 100 110 120 MYFLLGITLL RSYMQSVWTE EAQCALLNVS ITETFNCSFS CGPDCWKLSQ YPCLQVYVNL 130 140 150 160 170 180 TSSGERLLLY HTEETMKINQ KCSYIPKCGN NFEESMSLVS VVMENFRRHQ HFPCYSDPEG 190 200 210 220 230 NQKSVILTKL YSSNVLFHSL FWPTCMMAGG VAIVAMVKLT QYLSLLCERI QRINR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse kcnmb2 subunit of the large conductance calcium-activated K channel (MaxiK, BK)." Garcia-Valdes J., Eghbali M., Stefani E., Toro L. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Kidney.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY062429 mRNA. Translation: AAL38982.1. AK012400 mRNA. Translation: BAB28216.1. BC046227 mRNA. Translation: AAH46227.1. BC058957 mRNA. Translation: AAH58957.1.
IPI	IPI00112892.
RefSeq	NP_082507.1. NM_028231.2.
UniGene	Mm.64790.
3D structure databases
ProteinModelPortal	Q9CZM9.
SMR	Q9CZM9. Positions 1-45.
ModBase	Search...
PTM databases
PhosphoSite	Q9CZM9.
Proteomic databases
PRIDE	Q9CZM9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000119310; ENSMUSP00000112531; ENSMUSG00000037610. ENSMUST00000119970; ENSMUSP00000113234; ENSMUSG00000037610. ENSMUST00000178668; ENSMUSP00000136596; ENSMUSG00000037610.
GeneID	72413.
KEGG	mmu:72413.
UCSC	uc008owa.1. mouse.
Organism-specific databases
CTD	10242.
MGI	MGI:1919663. Kcnmb2.
Phylogenomic databases
eggNOG	NOG44064.
GeneTree	ENSGT00390000015997.
HOGENOM	HOG000290180.
HOVERGEN	HBG052223.
InParanoid	Q9CZM9.
KO	K04938.
OMA	VWTEEAQ.
OrthoDB	EOG4V438P.
Gene expression databases
Bgee	Q9CZM9.
Genevestigator	Q9CZM9.
GermOnline	ENSMUSG00000037610. Mus musculus.
Family and domain databases
Gene3D	4.10.81.20. 1 hit.
InterPro	IPR003930. K_chnl_Ca-activ_BK_bsu. IPR015382. KCNMB2_ball_chain_dom. [Graphical view]
PANTHER	PTHR10258. PTHR10258. 1 hit.
Pfam	PF03185. CaKB. 1 hit. PF09303. KcnmB2_inactiv. 1 hit. [Graphical view]
PRINTS	PR01450. BKCHANNELB.
ProtoNet	Search...
Other
NextBio	336214.
SOURCE	Search...

Entry information

Entry name

KCMB2_MOUSE

Accession

Primary (citable) accession number: Q9CZM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program