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Protein

Protein Mis18-alpha

Gene

Mis18a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561ZincPROSITE-ProRule annotation
Metal bindingi59 – 591ZincPROSITE-ProRule annotation
Metal bindingi112 – 1121ZincPROSITE-ProRule annotation
Metal bindingi115 – 1151ZincPROSITE-ProRule annotation

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • CENP-A containing nucleosome assembly Source: MGI
  • chromosome segregation Source: MGI
  • mitotic nuclear division Source: UniProtKB-KW
  • positive regulation of protein targeting to mitochondrion Source: MGI
  • regulation of DNA methylation Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-606279. Deposition of new CENPA-containing nucleosomes at the centromere.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Mis18-alpha
Gene namesi
Name:Mis18a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1913828. Mis18a.

Subcellular locationi

  • Nucleus By similarity
  • Chromosome By similarity
  • Chromosomecentromere By similarity

  • Note: Associated with centromeres in interphase cells, from late anaphase to the G1 phase. Not detected on centromeres during earlier phases of mitosis. Associated with chromatin.By similarity

GO - Cellular componenti

  • chromosome, centromeric region Source: MGI
  • cytoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204Protein Mis18-alphaPRO_0000278473Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei13 – 131PhosphoserineBy similarity
Modified residuei204 – 2041PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9CZJ6.
PaxDbiQ9CZJ6.
PRIDEiQ9CZJ6.

PTM databases

iPTMnetiQ9CZJ6.
PhosphoSiteiQ9CZJ6.

Expressioni

Gene expression databases

BgeeiQ9CZJ6.
CleanExiMM_2610039C10RIK.
GenevisibleiQ9CZJ6. MM.

Interactioni

Subunit structurei

Homodimer, and heterodimer with OIP5/MIS18B. Identified in a complex containing MIS18A, OIP5/MIS18B, MIS18BP1, RBBP7 and RBBP4.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000097150.

Structurei

3D structure databases

ProteinModelPortaliQ9CZJ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini51 – 14999Mis18PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the mis18 family.PROSITE-ProRule annotation
Contains 1 Mis18 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IKYV. Eukaryota.
ENOG41125JV. LUCA.
GeneTreeiENSGT00390000009777.
HOGENOMiHOG000059535.
HOVERGENiHBG108147.
InParanoidiQ9CZJ6.
KOiK11564.
OMAiEDSSRHQ.
OrthoDBiEOG70KGSQ.
PhylomeDBiQ9CZJ6.
TreeFamiTF333200.

Family and domain databases

InterProiIPR004910. Yippee/Mis18/Cereblon.
[Graphical view]
PfamiPF03226. Yippee-Mis18. 1 hit.
[Graphical view]
PROSITEiPS51793. MIS18. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CZJ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSESPLLEK RLSEDSSRYL RLQKWANMSS ADALGLEKER PEEKAAAAEN
60 70 80 90 100
PLVFLCARCR RPLGDSLTWV ASQEDTNCIL LRSVSCNVSV DKEPKLSKCR
110 120 130 140 150
DEDGCILEAL YCTGCSLSLG YVYRCTPKNL DYKRDLFCLS VEAVESYTLG
160 170 180 190 200
SSEKQIVSED KELFNLESRV EIEKSIKQME EVLTALQKKL REVESKLSLA

SRGS
Length:204
Mass (Da):22,949
Last modified:June 1, 2001 - v1
Checksum:iBBD667250FDF9B0F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012533 mRNA. Translation: BAB28302.1.
AK163310 mRNA. Translation: BAE37292.1.
BC079900 mRNA. Translation: AAH79900.1.
CCDSiCCDS28316.1.
RefSeqiNP_079918.1. NM_025642.1.
UniGeneiMm.180363.

Genome annotation databases

EnsembliENSMUST00000099554; ENSMUSP00000097150; ENSMUSG00000022978.
GeneIDi66578.
KEGGimmu:66578.
UCSCiuc007zwj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012533 mRNA. Translation: BAB28302.1.
AK163310 mRNA. Translation: BAE37292.1.
BC079900 mRNA. Translation: AAH79900.1.
CCDSiCCDS28316.1.
RefSeqiNP_079918.1. NM_025642.1.
UniGeneiMm.180363.

3D structure databases

ProteinModelPortaliQ9CZJ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000097150.

PTM databases

iPTMnetiQ9CZJ6.
PhosphoSiteiQ9CZJ6.

Proteomic databases

MaxQBiQ9CZJ6.
PaxDbiQ9CZJ6.
PRIDEiQ9CZJ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000099554; ENSMUSP00000097150; ENSMUSG00000022978.
GeneIDi66578.
KEGGimmu:66578.
UCSCiuc007zwj.1. mouse.

Organism-specific databases

CTDi54069.
MGIiMGI:1913828. Mis18a.

Phylogenomic databases

eggNOGiENOG410IKYV. Eukaryota.
ENOG41125JV. LUCA.
GeneTreeiENSGT00390000009777.
HOGENOMiHOG000059535.
HOVERGENiHBG108147.
InParanoidiQ9CZJ6.
KOiK11564.
OMAiEDSSRHQ.
OrthoDBiEOG70KGSQ.
PhylomeDBiQ9CZJ6.
TreeFamiTF333200.

Enzyme and pathway databases

ReactomeiR-MMU-606279. Deposition of new CENPA-containing nucleosomes at the centromere.

Miscellaneous databases

PROiQ9CZJ6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CZJ6.
CleanExiMM_2610039C10RIK.
GenevisibleiQ9CZJ6. MM.

Family and domain databases

InterProiIPR004910. Yippee/Mis18/Cereblon.
[Graphical view]
PfamiPF03226. Yippee-Mis18. 1 hit.
[Graphical view]
PROSITEiPS51793. MIS18. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Egg.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Eye.

Entry informationi

Entry nameiMS18A_MOUSE
AccessioniPrimary (citable) accession number: Q9CZJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: June 1, 2001
Last modified: June 8, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.