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Protein

Apoptosis-enhancing nuclease

Gene

Aen

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Exonuclease with activity against single- and double-stranded DNA and RNA. Mediates p53-induced apoptosis. When induced by p53 following DNA damage, digests double-stranded DNA to form single-stranded DNA and amplifies DNA damage signals, leading to enhancement of apoptosis (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Apoptosis, DNA damage

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptosis-enhancing nuclease (EC:3.1.-.-)
Alternative name(s):
Interferon-stimulated 20 kDa exonuclease-like 1
Gene namesi
Name:Aen
Synonyms:Isg20l1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1915298. Aen.

Subcellular locationi

  • Nucleus By similarity
  • Nucleusnucleolus By similarity

  • Note: Localized predomintly in the nucleolus. Translocates from the nucleolus to the nucleoplasm upon apoptosis induction (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 336336Apoptosis-enhancing nucleasePRO_0000324089Add
BLAST

Proteomic databases

EPDiQ9CZI9.
MaxQBiQ9CZI9.
PaxDbiQ9CZI9.
PRIDEiQ9CZI9.

PTM databases

iPTMnetiQ9CZI9.
PhosphoSiteiQ9CZI9.

Expressioni

Gene expression databases

BgeeiQ9CZI9.
ExpressionAtlasiQ9CZI9. baseline and differential.
GenevisibleiQ9CZI9. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000103046.

Structurei

3D structure databases

ProteinModelPortaliQ9CZI9.
SMRiQ9CZI9. Positions 110-269.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini109 – 265157ExonucleaseAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi25 – 339Nucleolar localization signalBy similarity
Motifi164 – 18724Nuclear localization signalBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 exonuclease domain.Curated

Phylogenomic databases

eggNOGiKOG2249. Eukaryota.
COG0847. LUCA.
GeneTreeiENSGT00520000055542.
HOGENOMiHOG000182422.
HOVERGENiHBG100435.
InParanoidiQ9CZI9.
KOiK18340.
OMAiGPLPSKC.
OrthoDBiEOG7WHHB0.
PhylomeDBiQ9CZI9.
TreeFamiTF354340.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00929. RNase_T. 1 hit.
[Graphical view]
SMARTiSM00479. EXOIII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CZI9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVAGEVPEST QCPSLTSLNT KDVVRRRHKR RSRQHQRFMA RKALLQEQEL
60 70 80 90 100
LSTAPGPGLC LLPSPSQMPA VTEASDSRRQ RPKARSGSNG LCSKKSVPRE
110 120 130 140 150
APRPGPIKCV AIDCEMVGTG PQGRVSELAR CSVVSYSGDV LYDKYIRPEM
160 170 180 190 200
PIVDYRTRWS GITRQHMHKA IPFQVAQKEI LKLLKGKVVV GHALHNDFQA
210 220 230 240 250
LKYVHPRSQT RDTTYVPNLL SQPSSLIRTR VSLKDLALNL LHKKIQVGHQ
260 270 280 290 300
GHSSVEDAMT AMELYQLVEV QWEQQVASSA QAPAEDRGPD SSTDVEQYMD
310 320 330
DQYWPEDLTQ STGAETNGGP DRQEGEEGQG ARSAPP
Length:336
Mass (Da):37,309
Last modified:June 1, 2001 - v1
Checksum:i0BE9173A65D7F018
GO
Isoform 2 (identifier: Q9CZI9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.

Show »
Length:298
Mass (Da):32,835
Checksum:i144200BF23D7AF68
GO

Sequence cautioni

The sequence BAC27632.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti62 – 621L → P in BAC41071 (PubMed:16141072).Curated
Sequence conflicti109 – 1091C → R in BAC41071 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3838Missing in isoform 2. 1 PublicationVSP_032133Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK090056 mRNA. Translation: BAC41071.1.
AK012555 mRNA. Translation: BAB28314.1.
AK031972 mRNA. Translation: BAC27632.1. Different initiation.
AK165708 mRNA. Translation: BAE38348.1.
AK166806 mRNA. Translation: BAE39034.1.
AK167042 mRNA. Translation: BAE39210.1.
AK167658 mRNA. Translation: BAE39708.1.
BC022614 mRNA. Translation: AAH22614.1.
CCDSiCCDS39988.2. [Q9CZI9-1]
CCDS52277.1. [Q9CZI9-2]
RefSeqiNP_001156411.1. NM_001162939.1. [Q9CZI9-2]
NP_080807.3. NM_026531.4. [Q9CZI9-1]
XP_006541200.1. XM_006541137.1. [Q9CZI9-1]
UniGeneiMm.34109.

Genome annotation databases

EnsembliENSMUST00000107421; ENSMUSP00000103044; ENSMUSG00000030609. [Q9CZI9-2]
ENSMUST00000107423; ENSMUSP00000103046; ENSMUSG00000030609. [Q9CZI9-1]
ENSMUST00000107425; ENSMUSP00000103048; ENSMUSG00000030609. [Q9CZI9-1]
GeneIDi68048.
KEGGimmu:68048.
UCSCiuc009hxp.1. mouse. [Q9CZI9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK090056 mRNA. Translation: BAC41071.1.
AK012555 mRNA. Translation: BAB28314.1.
AK031972 mRNA. Translation: BAC27632.1. Different initiation.
AK165708 mRNA. Translation: BAE38348.1.
AK166806 mRNA. Translation: BAE39034.1.
AK167042 mRNA. Translation: BAE39210.1.
AK167658 mRNA. Translation: BAE39708.1.
BC022614 mRNA. Translation: AAH22614.1.
CCDSiCCDS39988.2. [Q9CZI9-1]
CCDS52277.1. [Q9CZI9-2]
RefSeqiNP_001156411.1. NM_001162939.1. [Q9CZI9-2]
NP_080807.3. NM_026531.4. [Q9CZI9-1]
XP_006541200.1. XM_006541137.1. [Q9CZI9-1]
UniGeneiMm.34109.

3D structure databases

ProteinModelPortaliQ9CZI9.
SMRiQ9CZI9. Positions 110-269.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000103046.

PTM databases

iPTMnetiQ9CZI9.
PhosphoSiteiQ9CZI9.

Proteomic databases

EPDiQ9CZI9.
MaxQBiQ9CZI9.
PaxDbiQ9CZI9.
PRIDEiQ9CZI9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000107421; ENSMUSP00000103044; ENSMUSG00000030609. [Q9CZI9-2]
ENSMUST00000107423; ENSMUSP00000103046; ENSMUSG00000030609. [Q9CZI9-1]
ENSMUST00000107425; ENSMUSP00000103048; ENSMUSG00000030609. [Q9CZI9-1]
GeneIDi68048.
KEGGimmu:68048.
UCSCiuc009hxp.1. mouse. [Q9CZI9-1]

Organism-specific databases

CTDi64782.
MGIiMGI:1915298. Aen.

Phylogenomic databases

eggNOGiKOG2249. Eukaryota.
COG0847. LUCA.
GeneTreeiENSGT00520000055542.
HOGENOMiHOG000182422.
HOVERGENiHBG100435.
InParanoidiQ9CZI9.
KOiK18340.
OMAiGPLPSKC.
OrthoDBiEOG7WHHB0.
PhylomeDBiQ9CZI9.
TreeFamiTF354340.

Miscellaneous databases

PROiQ9CZI9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CZI9.
ExpressionAtlasiQ9CZI9. baseline and differential.
GenevisibleiQ9CZI9. MM.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00929. RNase_T. 1 hit.
[Graphical view]
SMARTiSM00479. EXOIII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Salivary gland.

Entry informationi

Entry nameiAEN_MOUSE
AccessioniPrimary (citable) accession number: Q9CZI9
Secondary accession number(s): Q3TKV9, Q8BTJ8, Q8C095
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 1, 2001
Last modified: June 8, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.